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The map label for this gene is dpnA

Identifier: 225859611

GI number: 225859611

Start: 1769102

End: 1769872

Strand: Reverse

Name: dpnA

Synonym: SP70585_1904

Alternate gene names: 225859611

Gene position: 1769872-1769102 (Counterclockwise)

Preceding gene: 225859612

Following gene: 225859610

Centisome position: 81.01

GC content: 34.37

Gene sequence:

>771_bases
ATGACAAAACCATACTACAATAAAAATAAGATGATTCTTGTTCATTCAGATACGTTCAAGTTCTTATCAAAAATGAAACC
AGAAAGTATGGATATGATTTTTGCTGATCCACCTTATTTTTTAAGTAATGGTGGAATATCTAATTCTGGGGGACAAGTTG
TTTCTGTTGATAAAGGAGATTGGGATAAAATTTCTTCATTCGAAGAAAAACATGAGTTTAATCGTAAATGGATTCGCCTA
GCAAAAGAAGTTCTGAAGCCTAATGGGACGGTATGGATTTCAGGTAGTTTGCACAACATATACTCAGTTGGAATGGCATT
AGAACAAGAAGGTTTTAAAATTCTGAATAATATTACTTGGCAGAAAACAAACCCTGCCCCCAATTTATCTTGTCGTTATT
TTACCCATTCTACTGAAACCATTTTATGGGCCAGAAAAAATGATAAAAAAGCTCGTCATTACTACAATTATGATTTAATG
AAAGAATTGAATGATGGAAAACAAATGAAAGATGTCTGGACGGGTTCTTTAACAAAGAAAGTTGAAAAATGGGCTGGGAA
ACATCCAACTCAAAAACCAGAGTATTTGTTAGAACGCATTATTTTAGCCTCTACTAAAGAGGGTGACTATATTCTAGACC
CATTTGTTGGTAGTGGCACTACGGGTGTTGTTGCGAAGCGGTTAGGTAGAAGATTTATAGGTATCGATGCTGAAAAAGAA
TATTTAAAAATTGCAAGGAAGAGGTTGGAGGCAGAAAATGAAACAAACTAG

Upstream 100 bases:

>100_bases
ACCCGAACTAATGGAGCAAAATCTTCAAGTCGAGGAAAAATTTCTGAAATTATAGTCACAAATTATGAAAAATAACGAAT
ATAAGTATGGAGGTGTTCTT

Downstream 100 bases:

>100_bases
AAATTTTGATGAGTGGTTGTCAACGATGACCGACACAGTAGCTGACTGGACTTATTATACAGATTTTCCCAAGGTTTATA
AAAATGTATCTAGTATAAAA

Product: DNA methylase

Products: NA

Alternate protein names: M.DpnIIB; Adenine-specific methyltransferase DpnIIB; M.DpnII 2

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MTKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNGGISNSGGQVVSVDKGDWDKISSFEEKHEFNRKWIRL
AKEVLKPNGTVWISGSLHNIYSVGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLM
KELNDGKQMKDVWTGSLTKKVEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGSGTTGVVAKRLGRRFIGIDAEKE
YLKIARKRLEAENETN

Sequences:

>Translated_256_residues
MTKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNGGISNSGGQVVSVDKGDWDKISSFEEKHEFNRKWIRL
AKEVLKPNGTVWISGSLHNIYSVGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLM
KELNDGKQMKDVWTGSLTKKVEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGSGTTGVVAKRLGRRFIGIDAEKE
YLKIARKRLEAENETN
>Mature_255_residues
TKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNGGISNSGGQVVSVDKGDWDKISSFEEKHEFNRKWIRLA
KEVLKPNGTVWISGSLHNIYSVGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLMK
ELNDGKQMKDVWTGSLTKKVEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGSGTTGVVAKRLGRRFIGIDAEKEY
LKIARKRLEAENETN

Specific function: Essential for establishment of a previously unmethylated plasmid transformed into the cell as single stranded DNA. Recognizes the single- or double-stranded sequence GATC, causes specific methylation on A-2 on one or both strands (respectively), and prote

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family

Homologues:

Organism=Escherichia coli, GI87082238, Length=258, Percent_Identity=31.7829457364341, Blast_Score=109, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTD22_STRPN (P09358)

Other databases:

- EMBL:   M14339
- PIR:   A24372
- ProteinModelPortal:   P09358
- SMR:   P09358
- REBASE:   3637
- BRENDA:   2.1.1.72
- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091
- PRINTS:   PR00508

Pfam domain/function: PF01555 N6_N4_Mtase

EC number: =2.1.1.72

Molecular weight: Translated: 29525; Mature: 29394

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNGGISNSGGQVVSVDKGD
CCCCEECCCCEEEEECHHHHHHHHCCCCCCCEEECCCCCEEECCCCCCCCCEEEEECCCC
WDKISSFEEKHEFNRKWIRLAKEVLKPNGTVWISGSLHNIYSVGMALEQEGFKILNNITW
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCC
QKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLMKELNDGKQMKDVWTGSLTKK
CCCCCCCCCEEEEEECCCCEEEEEECCCCCHHHHCCHHHHHHHCCCHHHHHHHHHHHHHH
VEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGSGTTGVVAKRLGRRFIGIDAEKE
HHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHCCCEECCCCHHH
YLKIARKRLEAENETN
HHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNGGISNSGGQVVSVDKGD
CCCEECCCCEEEEECHHHHHHHHCCCCCCCEEECCCCCEEECCCCCCCCCEEEEECCCC
WDKISSFEEKHEFNRKWIRLAKEVLKPNGTVWISGSLHNIYSVGMALEQEGFKILNNITW
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCC
QKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLMKELNDGKQMKDVWTGSLTKK
CCCCCCCCCEEEEEECCCCEEEEEECCCCCHHHHCCHHHHHHHCCCHHHHHHHHHHHHHH
VEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGSGTTGVVAKRLGRRFIGIDAEKE
HHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHCCCEECCCCHHH
YLKIARKRLEAENETN
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3019562; 10712690; 2824782