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The map label for this gene is dpnB [H]

Identifier: 225859610

GI number: 225859610

Start: 1768249

End: 1769115

Strand: Reverse

Name: dpnB [H]

Synonym: SP70585_1903

Alternate gene names: 225859610

Gene position: 1769115-1768249 (Counterclockwise)

Preceding gene: 225859611

Following gene: 225859606

Centisome position: 80.98

GC content: 29.07

Gene sequence:

>867_bases
ATGAAACAAACTAGAAATTTTGATGAGTGGTTGTCAACGATGACCGACACAGTAGCTGACTGGACTTATTATACAGATTT
TCCCAAGGTTTATAAAAATGTATCTAGTATAAAAGTTGCTCTAAATATTATGAATAGTCTTATTGGTAGTAAAAATATAC
AAGAGGATTTTTTAGACTTATATCAAAATTATCCAGAAATTTTGAAAGTTGTGCCTCTACTTATTGCTAAACGTTTGAGA
GATACGATTATTGTTAAGGATCCGATAAAAGATTTCTATTTTGATTTTAGTAAACGAAACTATAGTATTGAAGAATATAC
TATGTTTTTGGAAAAATCAGGTATATTTGATTTATTGCAAAATCATTTAGTATCAAATTTAGTTGACTACGTTACAGGTG
TTGAGGTTGGTATGGATACTAATGGGCGCAAAAATCGGACTGGAGATGCGATGGAAAATATTGTTCAAAGTTATCTTGAA
GCTGAAGGGTATATTTTAGGAGAGAATTTATTTAAAGAGATTGAACAAAATGAAATAGAGGAAATTTTTTCTGTCGATTT
ATCTGCTATTACGAATGATGGGAATACTGTGAAACGTTTTGATTTTGTCATAAAAAATGAACAAGTGCTTTATCTGATTG
AGGTTAATTTTTATTCAGGAAGTGGTTCAAAATTAAATGAGACGGCTAGATCTTACAAAATGATTGCTGAAGAAATTAAA
GCTATTCCTAATGTTGAGTTTATGTGGATTACTGATGGACAAGGTTGGTATAAGGCTAAGAATAATTTACGAGAAACATT
TGATATTTTACCATTCTTATATAATATTAACGATTTGGAACATAATATTTTAAAGAATTTGAAATAG

Upstream 100 bases:

>100_bases
ACTACGGGTGTTGTTGCGAAGCGGTTAGGTAGAAGATTTATAGGTATCGATGCTGAAAAAGAATATTTAAAAATTGCAAG
GAAGAGGTTGGAGGCAGAAA

Downstream 100 bases:

>100_bases
GTCCAATTCAATTAGATGGGAATTGGGATTGATTGTATTTATAATCAATTTGTGATAAACAAAAAAGAGGCTCGCACCTC
TTTTTCTTATTTCTTTTTAT

Product: type II restriction enzyme

Products: NA

Alternate protein names: R.DpnII; Endonuclease DpnII; Type II restriction enzyme DpnII [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVALNIMNSLIGSKNIQEDFLDLYQNYPEILKVVPLLIAKRLR
DTIIVKDPIKDFYFDFSKRNYSIEEYTMFLEKSGIFDLLQNHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMENIVQSYLE
AEGYILGENLFKEIEQNEIEEIFSVDLSAITNDGNTVKRFDFVIKNEQVLYLIEVNFYSGSGSKLNETARSYKMIAEEIK
AIPNVEFMWITDGQGWYKAKNNLRETFDILPFLYNINDLEHNILKNLK

Sequences:

>Translated_288_residues
MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVALNIMNSLIGSKNIQEDFLDLYQNYPEILKVVPLLIAKRLR
DTIIVKDPIKDFYFDFSKRNYSIEEYTMFLEKSGIFDLLQNHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMENIVQSYLE
AEGYILGENLFKEIEQNEIEEIFSVDLSAITNDGNTVKRFDFVIKNEQVLYLIEVNFYSGSGSKLNETARSYKMIAEEIK
AIPNVEFMWITDGQGWYKAKNNLRETFDILPFLYNINDLEHNILKNLK
>Mature_288_residues
MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVALNIMNSLIGSKNIQEDFLDLYQNYPEILKVVPLLIAKRLR
DTIIVKDPIKDFYFDFSKRNYSIEEYTMFLEKSGIFDLLQNHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMENIVQSYLE
AEGYILGENLFKEIEQNEIEEIFSVDLSAITNDGNTVKRFDFVIKNEQVLYLIEVNFYSGSGSKLNETARSYKMIAEEIK
AIPNVEFMWITDGQGWYKAKNNLRETFDILPFLYNINDLEHNILKNLK

Specific function: Recognizes the double-stranded unmethylated sequence GATC and cleaves before G-1 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II restriction enzyme DpnII family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021191
- InterPro:   IPR007637 [H]

Pfam domain/function: PF04556 DpmII [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 33598; Mature: 33598

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVALNIMNSLIGSKNIQEDFLDL
CCCCCCHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
YQNYPEILKVVPLLIAKRLRDTIIVKDPIKDFYFDFSKRNYSIEEYTMFLEKSGIFDLLQ
HHCHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHH
NHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMENIVQSYLEAEGYILGENLFKEIEQNEIE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEECHHHHHHHHHHHHH
EIFSVDLSAITNDGNTVKRFDFVIKNEQVLYLIEVNFYSGSGSKLNETARSYKMIAEEIK
HHHHHHHHHHCCCCCHHHHHHEEEECCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHH
AIPNVEFMWITDGQGWYKAKNNLRETFDILPFLYNINDLEHNILKNLK
HCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure
MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVALNIMNSLIGSKNIQEDFLDL
CCCCCCHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
YQNYPEILKVVPLLIAKRLRDTIIVKDPIKDFYFDFSKRNYSIEEYTMFLEKSGIFDLLQ
HHCHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHH
NHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMENIVQSYLEAEGYILGENLFKEIEQNEIE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEECHHHHHHHHHHHHH
EIFSVDLSAITNDGNTVKRFDFVIKNEQVLYLIEVNFYSGSGSKLNETARSYKMIAEEIK
HHHHHHHHHHCCCCCHHHHHHEEEECCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHH
AIPNVEFMWITDGQGWYKAKNNLRETFDILPFLYNINDLEHNILKNLK
HCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3019562; 2824782 [H]