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The map label for this gene is rsmE [H]

Identifier: 225859537

GI number: 225859537

Start: 1701283

End: 1702026

Strand: Reverse

Name: rsmE [H]

Synonym: SP70585_1827

Alternate gene names: 225859537

Gene position: 1702026-1701283 (Counterclockwise)

Preceding gene: 225859538

Following gene: 225859536

Centisome position: 77.91

GC content: 44.89

Gene sequence:

>744_bases
ATGCAACAGTATTTTGTCAAAGGCAGTGCTATCTCTCCTGTCACCATCGAGGACAAGGAAACCAGTAAGCATATGTTTCA
GGTTATGCGCTTGAAAGAAGAGGATGAGGTTACCTTAGTCTTTGATGATGGCATCAAGCGCTTGGCGCGCGTGCTGGATA
TGGAAAATCGTCAGTTTGAGTTGGTCCAAGAATTAAATGACAATGTGGAACTACCAGTCCAAGTGACCATCGCATCTGGA
TTTCCCAAGGGAGACAAGCTGGAGTTCATCGCTCAAAAAGTAACCGAACTGGGTGCTAGCCAAATCTGGGCCTTTCCTGC
AGACTGGTCAGTTGCCAAGTGGGATGGCAAGAAATTGGGTAAAAAAGTTGAAAAACTAGAAAAAATTGCCCTTGGAGCAG
CCGAGCAAAGCAAGCGTAATATTGTACCAAGTATTAAACTTTTCGAGAAAAAAGCAGATTTTCTAGCTCAGCTGGACCAG
TTTGACTCTATCATAGTAGCCTATGAAGAATCAGCTAAAGAAGGAGAAGCCGCTGCGCTCTTACAAGCGGTCTCTGGTCT
TGAAACAGGAGCCAAACTGCTCTTTATCTTTGGACCAGAAGGCGGTCTCTCACCTGCAGAAATCGAAAGTTTTGAAGCTA
AGGGAGCCGTTTTGGTAGGCCTAGGTCCTCGTATTTTGCGAGCAGAAACAGCACCGCTTTACGCCTTAACAGCCCTTAGT
GTTTTAGTAGAATTAGAGAAATAA

Upstream 100 bases:

>100_bases
TCAGCTGGATTTTTCCTTGAAACCCATATGATTCAAGGGGAATGGAATGCCTGCGTCTTCAAGAAGACCAAGGATATTTC
AGGTGTGATTGGAGGCTAGG

Downstream 100 bases:

>100_bases
GAGGAAGAAAATGGAACAAAAACACCGTTCAGAATTTCCAGAGAAGGAACTCTGGGACTTAACAGCCCTATACCAAGACC
GTGAGGATTTCTTGCGTGCA

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDGIKRLARVLDMENRQFELVQELNDNVELPVQVTIASG
FPKGDKLEFIAQKVTELGASQIWAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
FDSIIVAYEESAKEGEAAALLQAVSGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVLVGLGPRILRAETAPLYALTALS
VLVELEK

Sequences:

>Translated_247_residues
MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDGIKRLARVLDMENRQFELVQELNDNVELPVQVTIASG
FPKGDKLEFIAQKVTELGASQIWAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
FDSIIVAYEESAKEGEAAALLQAVSGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVLVGLGPRILRAETAPLYALTALS
VLVELEK
>Mature_247_residues
MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDGIKRLARVLDMENRQFELVQELNDNVELPVQVTIASG
FPKGDKLEFIAQKVTELGASQIWAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
FDSIIVAYEESAKEGEAAALLQAVSGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVLVGLGPRILRAETAPLYALTALS
VLVELEK

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=176, Percent_Identity=32.3863636363636, Blast_Score=84, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27184; Mature: 27184

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDGIKRLARVLDMENRQFE
CCCEEECCCCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCCCCCHHH
LVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQIWAFPADWSVAKWDGKKLG
HHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCHHEEECCCCCCEEECCHHHHH
KKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQFDSIIVAYEESAKEGEAAAL
HHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHH
LQAVSGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVLVGLGPRILRAETAPLYALTALS
HHHHHCCCCCCEEEEEECCCCCCCHHHHHHHCCCCEEEEECCCCEEECCCCCHHHHHHHH
VLVELEK
HHHHCCC
>Mature Secondary Structure
MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDGIKRLARVLDMENRQFE
CCCEEECCCCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCCCCCHHH
LVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQIWAFPADWSVAKWDGKKLG
HHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCHHEEECCCCCCEEECCHHHHH
KKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQFDSIIVAYEESAKEGEAAAL
HHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHH
LQAVSGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVLVGLGPRILRAETAPLYALTALS
HHHHHCCCCCCEEEEEECCCCCCCHHHHHHHCCCCEEEEECCCCEEECCCCCHHHHHHHH
VLVELEK
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9023197; 9384377 [H]