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The map label for this gene is nisR [H]

Identifier: 225859022

GI number: 225859022

Start: 1193715

End: 1194398

Strand: Reverse

Name: nisR [H]

Synonym: SP70585_1290

Alternate gene names: 225859022

Gene position: 1194398-1193715 (Counterclockwise)

Preceding gene: 225859023

Following gene: 225859021

Centisome position: 54.67

GC content: 32.31

Gene sequence:

>684_bases
ATGAAAGAAAAAAAAGTATATCAAATATTAGCAATTGATGATGATGAAAGCATTCTTCGTTTGATAAAGAATTCTTTAGA
ACGCTCGGAGTTTGAAGTTACGACGAGGAGAGAAGTAGGTAACATAGATATTTGTTCATTCATTGGCTTTGATTTAATTT
TATTAGATATTATGATGTCGATTGATGGTTTAGAAATTTGTAAATCCATTCGAGAACAAATCGATGTACCGATAATTTTT
GTAACAGCCAAGCAATTGGATAGTGACTTGGCTAGTGGAGTAAAAGCTGGTGCGGATGATTATATAAAAAAACCATTTAG
TATTACAGAATTAGTTGCTCGTGTTAGGATGCATATCCAAAGGGAGGAAAGAAATCGTAAAAATAATAAAGAAATATGCA
CAAAGAATTTGATAATTAATGTCAGCAATCAAGAAATCTTAATTGAAGAAGAGACTATATCATTGACAAAGCGAGAATTT
ACAATTTTATATACTTTAGCAAGCAACCCGAAGAGAGTATATTCCGTAGAGGAGCTCTATGAGAGAATATATCCGTCGGA
ATCAGATGCACAATTTCGTTCTATTGCTGAGTATATTTATCAAATTCGTTCAAAATTAAAACCATATTCAATTAATCCGA
TCAAGACACAGTGGGGAGGCGGATATAGTTGGAACGAAAATTAG

Upstream 100 bases:

>100_bases
TCAATTTTTATGGGGGATTTTGTTGTTATCTTTCTCTATTTTGCTATTTTGGAAGAGAATAGTACGATAAAAATTTTGTA
TTTTGTGATATTATATAATT

Downstream 100 bases:

>100_bases
ATTTTAAGACTTTAGTAAAAAAGACGAAGTGGACGATTGTTAAACAATTTTGTTTGAATATAATTGTAGCTTATATTTTT
CCTATATCAACTATTTCTAT

Product: NisR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MKEKKVYQILAIDDDESILRLIKNSLERSEFEVTTRREVGNIDICSFIGFDLILLDIMMSIDGLEICKSIREQIDVPIIF
VTAKQLDSDLASGVKAGADDYIKKPFSITELVARVRMHIQREERNRKNNKEICTKNLIINVSNQEILIEEETISLTKREF
TILYTLASNPKRVYSVEELYERIYPSESDAQFRSIAEYIYQIRSKLKPYSINPIKTQWGGGYSWNEN

Sequences:

>Translated_227_residues
MKEKKVYQILAIDDDESILRLIKNSLERSEFEVTTRREVGNIDICSFIGFDLILLDIMMSIDGLEICKSIREQIDVPIIF
VTAKQLDSDLASGVKAGADDYIKKPFSITELVARVRMHIQREERNRKNNKEICTKNLIINVSNQEILIEEETISLTKREF
TILYTLASNPKRVYSVEELYERIYPSESDAQFRSIAEYIYQIRSKLKPYSINPIKTQWGGGYSWNEN
>Mature_227_residues
MKEKKVYQILAIDDDESILRLIKNSLERSEFEVTTRREVGNIDICSFIGFDLILLDIMMSIDGLEICKSIREQIDVPIIF
VTAKQLDSDLASGVKAGADDYIKKPFSITELVARVRMHIQREERNRKNNKEICTKNLIINVSNQEILIEEETISLTKREF
TILYTLASNPKRVYSVEELYERIYPSESDAQFRSIAEYIYQIRSKLKPYSINPIKTQWGGGYSWNEN

Specific function: Member of the two-component regulatory system nisK/nisR involved in the regulation of the biosynthesis of lantibiotic nisin. NisR may function as a regulatory protein [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789809, Length=228, Percent_Identity=33.3333333333333, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1788394, Length=180, Percent_Identity=36.1111111111111, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1790860, Length=177, Percent_Identity=31.638418079096, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI2367329, Length=228, Percent_Identity=32.0175438596491, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1786599, Length=224, Percent_Identity=28.125, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1786911, Length=232, Percent_Identity=26.7241379310345, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1789402, Length=223, Percent_Identity=27.8026905829596, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87082012, Length=177, Percent_Identity=29.9435028248588, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790552, Length=175, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787229, Length=179, Percent_Identity=32.4022346368715, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787375, Length=163, Percent_Identity=31.2883435582822, Blast_Score=76, Evalue=1e-15,
Organism=Escherichia coli, GI1790863, Length=205, Percent_Identity=29.7560975609756, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786784, Length=176, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26386; Mature: 26386

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKKVYQILAIDDDESILRLIKNSLERSEFEVTTRREVGNIDICSFIGFDLILLDIMMS
CCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
IDGLEICKSIREQIDVPIIFVTAKQLDSDLASGVKAGADDYIKKPFSITELVARVRMHIQ
CCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHH
REERNRKNNKEICTKNLIINVSNQEILIEEETISLTKREFTILYTLASNPKRVYSVEELY
HHHHCCCCCHHHHHHEEEEEECCCEEEEEHHHHHHHHEEEEEEEEECCCCHHHHHHHHHH
ERIYPSESDAQFRSIAEYIYQIRSKLKPYSINPIKTQWGGGYSWNEN
HHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKEKKVYQILAIDDDESILRLIKNSLERSEFEVTTRREVGNIDICSFIGFDLILLDIMMS
CCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
IDGLEICKSIREQIDVPIIFVTAKQLDSDLASGVKAGADDYIKKPFSITELVARVRMHIQ
CCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHH
REERNRKNNKEICTKNLIINVSNQEILIEEETISLTKREFTILYTLASNPKRVYSVEELY
HHHHCCCCCHHHHHHEEEEEECCCEEEEEHHHHHHHHEEEEEEEEECCCCHHHHHHHHHH
ERIYPSESDAQFRSIAEYIYQIRSKLKPYSINPIKTQWGGGYSWNEN
HHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8478324; 8161176 [H]