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The map label for this gene is nisK [H]

Identifier: 225859021

GI number: 225859021

Start: 1192633

End: 1193730

Strand: Reverse

Name: nisK [H]

Synonym: SP70585_1289

Alternate gene names: 225859021

Gene position: 1193730-1192633 (Counterclockwise)

Preceding gene: 225859022

Following gene: 225859020

Centisome position: 54.64

GC content: 29.87

Gene sequence:

>1098_bases
TTGGAACGAAAATTAGATTTTAAGACTTTAGTAAAAAAGACGAAGTGGACGATTGTTAAACAATTTTGTTTGAATATAAT
TGTAGCTTATATTTTTCCTATATCAACTATTTCTATTGATATTCATTCAGAAACTCAGTCAATCGCTCGTGACTTGTTAG
GGGTATTATTTAATGTTTTTTCATGGGTTTATATTTGTATATCTTTGATTATTATAGTTTCTATTAATATTAAAGCCTTG
TTGACAAGAGTAAAGCAAGAAATGGAGATTGTTTATCATAGCAGTATATGGCTAACCTCTGTAAACGATTCATCTAATTT
AACAATTCTTGAATTCATAGAGACAAATAGACACATTGAATCAATGCAACAAAGAATAAAAAATATGATTCAGGTAGAGA
AAGACCAAAAGGCGGATATTCTCTTTAAAGTTTCAGCGATGGCACATGACTTAAAAACACCTTTGACTGTGATTAAAGGA
AACTCGGAATTGTTGCAATTGGCACCTTTATCAGGTATTGATGTTCAATGTCTCAAGGATATTGAAAGAGCAAGTAACCA
ATTAAATAGCTATTTTAATCAGTTAATCAACTACTCTAAAACTTTTTACTCTGATCAGATTTCTTTTCATCAGTATCGTA
TCAGTAATTTATCCAAAATACTGGAACAAGAGTGTACATACCTTATTGGAGATAGCATTGACTACAAATATAGTACTAAG
ATAGACCGAGACTGTAATATTGAATTGGATTTAGATTTAATTATCCGTTCGGTGGCAAATATTGTTAATAATGCTATAGC
TTATGCAGATGATGCTAAAAAGAAAATCAGAGTTACCTTTGAGTTAGCAGATAGCAATATTACCTTAAGTATTTGGAATA
ATGGTTCCAAATTTTCGGATGATGTTTTAGAAAATTTTGGAAAATTATTTTATCGAGAAGATACTAGCAGAAATTCACAA
TTTGAACATTTTGGAATAGGTTTGGCATTTGTAAAACAAGTAATGAGCATTCATTCAGGTGATATACAATTGCAAAATAA
GGAAAATGGAGCTATGGTTAAATTAATTATTCCGATGAAAAGTAGTGACTATGTCTAG

Upstream 100 bases:

>100_bases
CACAATTTCGTTCTATTGCTGAGTATATTTATCAAATTCGTTCAAAATTAAAACCATATTCAATTAATCCGATCAAGACA
CAGTGGGGAGGCGGATATAG

Downstream 100 bases:

>100_bases
GCTTGAATATCGTAATTTTGAATACTATGAAGTTCAGAAAGTAAGTATATTAATAAAATTTATTTTAAAATTTATTTTAA
AATTTCAACAATTAGCACTC

Product: NisK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MERKLDFKTLVKKTKWTIVKQFCLNIIVAYIFPISTISIDIHSETQSIARDLLGVLFNVFSWVYICISLIIIVSINIKAL
LTRVKQEMEIVYHSSIWLTSVNDSSNLTILEFIETNRHIESMQQRIKNMIQVEKDQKADILFKVSAMAHDLKTPLTVIKG
NSELLQLAPLSGIDVQCLKDIERASNQLNSYFNQLINYSKTFYSDQISFHQYRISNLSKILEQECTYLIGDSIDYKYSTK
IDRDCNIELDLDLIIRSVANIVNNAIAYADDAKKKIRVTFELADSNITLSIWNNGSKFSDDVLENFGKLFYREDTSRNSQ
FEHFGIGLAFVKQVMSIHSGDIQLQNKENGAMVKLIIPMKSSDYV

Sequences:

>Translated_365_residues
MERKLDFKTLVKKTKWTIVKQFCLNIIVAYIFPISTISIDIHSETQSIARDLLGVLFNVFSWVYICISLIIIVSINIKAL
LTRVKQEMEIVYHSSIWLTSVNDSSNLTILEFIETNRHIESMQQRIKNMIQVEKDQKADILFKVSAMAHDLKTPLTVIKG
NSELLQLAPLSGIDVQCLKDIERASNQLNSYFNQLINYSKTFYSDQISFHQYRISNLSKILEQECTYLIGDSIDYKYSTK
IDRDCNIELDLDLIIRSVANIVNNAIAYADDAKKKIRVTFELADSNITLSIWNNGSKFSDDVLENFGKLFYREDTSRNSQ
FEHFGIGLAFVKQVMSIHSGDIQLQNKENGAMVKLIIPMKSSDYV
>Mature_365_residues
MERKLDFKTLVKKTKWTIVKQFCLNIIVAYIFPISTISIDIHSETQSIARDLLGVLFNVFSWVYICISLIIIVSINIKAL
LTRVKQEMEIVYHSSIWLTSVNDSSNLTILEFIETNRHIESMQQRIKNMIQVEKDQKADILFKVSAMAHDLKTPLTVIKG
NSELLQLAPLSGIDVQCLKDIERASNQLNSYFNQLINYSKTFYSDQISFHQYRISNLSKILEQECTYLIGDSIDYKYSTK
IDRDCNIELDLDLIIRSVANIVNNAIAYADDAKKKIRVTFELADSNITLSIWNNGSKFSDDVLENFGKLFYREDTSRNSQ
FEHFGIGLAFVKQVMSIHSGDIQLQNKENGAMVKLIIPMKSSDYV

Specific function: Member of the two-component regulatory system nisK/nisR involved in the regulation of the biosynthesis of lantibiotic nisin. NisK may function as a membrane-associated protein kinase that phosphorylates nisR in response to environmental signals [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786600, Length=260, Percent_Identity=22.3076923076923, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790861, Length=254, Percent_Identity=20.8661417322835, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788549, Length=238, Percent_Identity=23.9495798319328, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR008358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 41916; Mature: 41916

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERKLDFKTLVKKTKWTIVKQFCLNIIVAYIFPISTISIDIHSETQSIARDLLGVLFNVF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHH
SWVYICISLIIIVSINIKALLTRVKQEMEIVYHSSIWLTSVNDSSNLTILEFIETNRHIE
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEECEEEEEEECCCCCEEEEEEHHHCHHHH
SMQQRIKNMIQVEKDQKADILFKVSAMAHDLKTPLTVIKGNSELLQLAPLSGIDVQCLKD
HHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHCCCEEEEECCCCEEEECCCCCCCHHHHHH
IERASNQLNSYFNQLINYSKTFYSDQISFHQYRISNLSKILEQECTYLIGDSIDYKYSTK
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCEECCC
IDRDCNIELDLDLIIRSVANIVNNAIAYADDAKKKIRVTFELADSNITLSIWNNGSKFSD
CCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCEEEEEEECCCCCCHH
DVLENFGKLFYREDTSRNSQFEHFGIGLAFVKQVMSIHSGDIQLQNKENGAMVKLIIPMK
HHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECC
SSDYV
CCCCC
>Mature Secondary Structure
MERKLDFKTLVKKTKWTIVKQFCLNIIVAYIFPISTISIDIHSETQSIARDLLGVLFNVF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHH
SWVYICISLIIIVSINIKALLTRVKQEMEIVYHSSIWLTSVNDSSNLTILEFIETNRHIE
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEECEEEEEEECCCCCEEEEEEHHHCHHHH
SMQQRIKNMIQVEKDQKADILFKVSAMAHDLKTPLTVIKGNSELLQLAPLSGIDVQCLKD
HHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHCCCEEEEECCCCEEEECCCCCCCHHHHHH
IERASNQLNSYFNQLINYSKTFYSDQISFHQYRISNLSKILEQECTYLIGDSIDYKYSTK
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCEECCC
IDRDCNIELDLDLIIRSVANIVNNAIAYADDAKKKIRVTFELADSNITLSIWNNGSKFSD
CCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCEEEEEEECCCCCCHH
DVLENFGKLFYREDTSRNSQFEHFGIGLAFVKQVMSIHSGDIQLQNKENGAMVKLIIPMK
HHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECC
SSDYV
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8161176 [H]