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The map label for this gene is recN

Identifier: 225858987

GI number: 225858987

Start: 1162515

End: 1164182

Strand: Reverse

Name: recN

Synonym: SP70585_1252

Alternate gene names: 225858987

Gene position: 1164182-1162515 (Counterclockwise)

Preceding gene: 225858988

Following gene: 225858986

Centisome position: 53.29

GC content: 41.73

Gene sequence:

>1668_bases
ATGTTACTTGAAATTTCGATAAAAAACTTTGCCATTATTGAGGCTATTTCCCTCAATTTTGAAAAGGGGATGACTGTCCT
GACTGGTGAAACGGGTGCAGGGAAGTCGATTATCATTGATGCCATGAATATGATGTTGGGAGCTCGTGCGACGACAGATG
TTATTCGTCATGGTGCACCAAAGGCAGAGATTGAGGGGCTTTTCTCAGTTGAGAATAGTCGTCTTTTACAGGAAATTTTT
GATGAGCAAGGTTTAGAATTAGGTGATGAAATTATCATCCGTCGGGAAATCTTGCAAAATGGTCGTAGTATCAGCCGTGT
AAATGGTCAGATGGTTAATCTGTCTGTTTTGCGAACTATTGGGCAACATCTTGTAGATATTCATGGTCAGCATGACCATG
AGGAGTTAATGCGTCCCCAACTGCATATCCAGATGTTGGATGAATTTGGTGATGCCGCTTTTTGGGACTTGAAAGAAACC
TATCAAACGAGTTTTGATGCCTATCGGAAAATGCGCAAGCAGGTTCTGGAAGTCAAGAAAAACCAACAGGAACACAAGGC
ACGTATCGAAATGTTGGAATTTCAAATGGCAGAGATTGAGGCAGCAAACTTGCAGGCTGGAGAAGACTTGGATCTCAATC
AAGAGCGAGATAAACTCCTCAACCACAAAAATATTGCGGATACACTGACCAATGCCTACAGTATGTTGGACAATGAAGAT
TTTTCAAGTCTGGCCAATGTTCGTTCAGCCATGAATGACATGGAAAGTGTCGAAGAGTATGACCCTGAATACCGTGAAAT
TTCAAGCTCTCTGTCTGAGACCTACTATGTTTTAGAAGACATTAGCAAACGTTTGGAAGCTATCATTGAGGACCTTGATT
TTGATGGCAATCGCCTCATGCAGGTTGAGAATCGTTTGGACCTCCTTCATACTATTACTCGTAAGTATGGTGGGACTGTT
GATGATGTTTTGCTTTATTTTGCCAAGATTACGGAAGAATACAATCTCTTGACAGGCAATAATCTTTCGTCTGAGGACAT
GGAAGCAGAGCTTAAGAAGTTGGAAGTCAATCTTGTCAATTTGGCAGGTCAACTTGCTTCTGCTCGTCATAATTTGGCTC
AGCAACTCGAAGCTGAGATTAAACAAGAACTGCAAGATCTTTATATGGAAAAAGCCCAGTTTCAGGTTCGTTTTAGTAAG
GGAAAATTCAGTCGTGAGGGAAATAAAATGGTTGAGTTTTATATTTCAACCAACCCTGGAGAAGACTTTAAACCCTTGGT
TAAGGTTGCTTCTGGAGGGGAATTATCTCGTCTCATGTTAGCCATTAAGTCTGCCTTTTCACGTAAAGAAGGCAAGACTA
GCATTGTCTTTGATGAGGTGGATACGGGAGTTTCAGGTCGTGTTGCTCAAGCTATTGCTCAGAAAATTCATAAAATTGGT
CAGCATGGTCAGGTTTTGGCTATCTCCCATTTGCCACAAGTAATTGCGATTGCAGATTATCAATTCTTTATTGAGAAGAT
TAGTAATGACCATTCAACGGTTTCGACTGTTCGTCTCTTGACGGTCGAAGAGCGAGTGGAGGAAGTTGCCAAGATGTTGG
CAGGTGATGATGTGACAGAAGCAGCCCTGACGCAAGCCAGAGAATTGTTGAGAAACAGGGAGAAATAA

Upstream 100 bases:

>100_bases
TTTTAGCTGTTTGACTGATGATAGCTCGATTTTAGTCATTGCCAGAAGTGGAAGTCTAGCAGAGGAAATCTTTGAACAAG
TAAAAAATTGGTAGGTCTAT

Downstream 100 bases:

>100_bases
GATGACAGATTATTATGTAATTGGAGATGTTCACGGAAAAGCTGGGATGCTGGAAGACCTTCTTAAAACCTGGGATGGTC
AGACCCAGTTGCTCTTTCTA

Product: DNA repair protein RecN

Products: NA

Alternate protein names: Recombination protein N [H]

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNMMLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIF
DEQGLELGDEIIIRREILQNGRSISRVNGQMVNLSVLRTIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLDLNQERDKLLNHKNIADTLTNAYSMLDNED
FSSLANVRSAMNDMESVEEYDPEYREISSSLSETYYVLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTV
DDVLLYFAKITEEYNLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKAQFQVRFSK
GKFSREGNKMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKEGKTSIVFDEVDTGVSGRVAQAIAQKIHKIG
QHGQVLAISHLPQVIAIADYQFFIEKISNDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK

Sequences:

>Translated_555_residues
MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNMMLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIF
DEQGLELGDEIIIRREILQNGRSISRVNGQMVNLSVLRTIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLDLNQERDKLLNHKNIADTLTNAYSMLDNED
FSSLANVRSAMNDMESVEEYDPEYREISSSLSETYYVLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTV
DDVLLYFAKITEEYNLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKAQFQVRFSK
GKFSREGNKMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKEGKTSIVFDEVDTGVSGRVAQAIAQKIHKIG
QHGQVLAISHLPQVIAIADYQFFIEKISNDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK
>Mature_555_residues
MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNMMLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIF
DEQGLELGDEIIIRREILQNGRSISRVNGQMVNLSVLRTIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLDLNQERDKLLNHKNIADTLTNAYSMLDNED
FSSLANVRSAMNDMESVEEYDPEYREISSSLSETYYVLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTV
DDVLLYFAKITEEYNLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKAQFQVRFSK
GKFSREGNKMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKEGKTSIVFDEVDTGVSGRVAQAIAQKIHKIG
QHGQVLAISHLPQVIAIADYQFFIEKISNDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK

Specific function: Involved in recombinational repair of damaged DNA. Seems to be the first protein recruited to repair centers, foci that are the site of double-strand DNA break(s), followed by recO and then recF [H]

COG id: COG0497

COG function: function code L; ATPase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recN family [H]

Homologues:

Organism=Escherichia coli, GI48994901, Length=555, Percent_Identity=32.972972972973, Blast_Score=309, Evalue=2e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004604
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 62866; Mature: 62866

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNMMLGARATTDVIRHGAP
CEEEEEECCEEEHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCC
KAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGRSISRVNGQMVNLSVLRTI
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHH
GQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKETYQTSFDAYRKMRKQVLEVKK
HHHHHHHCCCCCHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NQQEHKARIEMLEFQMAEIEAANLQAGEDLDLNQERDKLLNHKNIADTLTNAYSMLDNED
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCH
FSSLANVRSAMNDMESVEEYDPEYREISSSLSETYYVLEDISKRLEAIIEDLDFDGNRLM
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
QVENRLDLLHTITRKYGGTVDDVLLYFAKITEEYNLLTGNNLSSEDMEAELKKLEVNLVN
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
LAGQLASARHNLAQQLEAEIKQELQDLYMEKAQFQVRFSKGKFSREGNKMVEFYISTNPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEEEEEECCCC
EDFKPLVKVASGGELSRLMLAIKSAFSRKEGKTSIVFDEVDTGVSGRVAQAIAQKIHKIG
CCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHC
QHGQVLAISHLPQVIAIADYQFFIEKISNDHSTVSTVRLLTVEERVEEVAKMLAGDDVTE
CCCCEEEHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AALTQARELLRNREK
HHHHHHHHHHHCCCC
>Mature Secondary Structure
MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNMMLGARATTDVIRHGAP
CEEEEEECCEEEHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCC
KAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGRSISRVNGQMVNLSVLRTI
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHH
GQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKETYQTSFDAYRKMRKQVLEVKK
HHHHHHHCCCCCHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NQQEHKARIEMLEFQMAEIEAANLQAGEDLDLNQERDKLLNHKNIADTLTNAYSMLDNED
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCH
FSSLANVRSAMNDMESVEEYDPEYREISSSLSETYYVLEDISKRLEAIIEDLDFDGNRLM
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
QVENRLDLLHTITRKYGGTVDDVLLYFAKITEEYNLLTGNNLSSEDMEAELKKLEVNLVN
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
LAGQLASARHNLAQQLEAEIKQELQDLYMEKAQFQVRFSKGKFSREGNKMVEFYISTNPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEEEEEECCCC
EDFKPLVKVASGGELSRLMLAIKSAFSRKEGKTSIVFDEVDTGVSGRVAQAIAQKIHKIG
CCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHC
QHGQVLAISHLPQVIAIADYQFFIEKISNDHSTVSTVRLLTVEERVEEVAKMLAGDDVTE
CCCCEEEHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AALTQARELLRNREK
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 2507400; 9044256 [H]