Click here to switch to the map view.

The map label for this gene is braE [H]

Identifier: 225686936

GI number: 225686936

Start: 1176156

End: 1177103

Strand: Direct

Name: braE [H]

Synonym: BMEA_B1190

Alternate gene names: 225686936

Gene position: 1176156-1177103 (Clockwise)

Preceding gene: 225686935

Following gene: 225686937

Centisome position: 99.21

GC content: 57.28

Gene sequence:

>948_bases
ATGAAGTATCTTCCAGCCCTTCTCGCCATCATTGCTGCTGTTCTGGCGCTAGGCGCGCCGCTGTTCGCCTCCCCGGTCAT
GATGCAGTTGATGTTCGATGCGCTCATAATCGCAACACTGGCCCAGGCCTGGAACATCATCGGTGGTTTCACCGGCTACG
CCTCCTTCGGCAATTCGGTCTTCTATGGTCTGGGTGCCTACGGCGCCGGCATCGCCATGGTGCAGTTCAACCTGCCGTTC
TTCGCAGGGGCCGCCATCGGCATCGTCGCAGCCGTTGTCTTTGCAATGCTCATCGGTTTGCCGATCCTGCGTCTCAAGGG
CCACTATTTCGCCATCGCCACGCTGGCGCTTCCGCAGGTCATGGCGGCCATCACGGCCAATGTCCCCGTAGCCGGGGAGA
ATATCGGCCTCGTCCTTCCCCTCCTCAACAACGACACTTTCTTCTATGAATCGGCGCTGATCCTCCTCATCATCGCCACC
TGTGTAGTGGCGCTGCTGACACGCTCGCGCTTCGGATTCGGGCTGATCGCCATTCGTGAAAATGAGGAAGGTGCCGCTGC
AATGGGCATCAACACCACATTGTACAAAATATTGGCTTTGATGCTGAGTGGTGCTTTCTGCGCCATGGCGGGTGCGCTCC
AGGCGTACAAGATCACCTTTCTGGACCCGGAAGCAGCATATCAGGTGCAGCTCAACGTACAGATGATTATCATGGCGGTG
TTCGGTGGCGCAGGCACAATTTTCGGGCCGGTCTTCGGTGCGCTTTTCCTGCAATGGGTGTCCAACTGGCTCTCCACTGC
CTATACCGACGTGGCAGGCATGTTCTTTGGCATAGTCATCGTTGTCGCGGTGGTTTTGATGCCACGCGGCCTTGCCGATC
TGATGCGCAATCTGCCGCGGCAGGGCTGGCGCTATTTCATCAACAACATGAAGGCGAATAAACTATGA

Upstream 100 bases:

>100_bases
CCGTGCTCTATCTCGGCGACACTTACCGCAACGTGGTCAGCTTCAGTATTCTCGTTCTCGTACTGATCATTCGGCCCGGT
GGGCTTTTGGGGAAAACGGC

Downstream 100 bases:

>100_bases
CCGCCCTGCTGGAAGTCAGGAACCTTACCAAACGCTTTGGGGGGCTGACAGCAGTCAACAATGTCTCTTTCACGTTGAAC
AAGGGAGAAATATTGGGCTT

Product: high-affinity branched-chain amino acid transporter membrane protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MKYLPALLAIIAAVLALGAPLFASPVMMQLMFDALIIATLAQAWNIIGGFTGYASFGNSVFYGLGAYGAGIAMVQFNLPF
FAGAAIGIVAAVVFAMLIGLPILRLKGHYFAIATLALPQVMAAITANVPVAGENIGLVLPLLNNDTFFYESALILLIIAT
CVVALLTRSRFGFGLIAIRENEEGAAAMGINTTLYKILALMLSGAFCAMAGALQAYKITFLDPEAAYQVQLNVQMIIMAV
FGGAGTIFGPVFGALFLQWVSNWLSTAYTDVAGMFFGIVIVVAVVLMPRGLADLMRNLPRQGWRYFINNMKANKL

Sequences:

>Translated_315_residues
MKYLPALLAIIAAVLALGAPLFASPVMMQLMFDALIIATLAQAWNIIGGFTGYASFGNSVFYGLGAYGAGIAMVQFNLPF
FAGAAIGIVAAVVFAMLIGLPILRLKGHYFAIATLALPQVMAAITANVPVAGENIGLVLPLLNNDTFFYESALILLIIAT
CVVALLTRSRFGFGLIAIRENEEGAAAMGINTTLYKILALMLSGAFCAMAGALQAYKITFLDPEAAYQVQLNVQMIIMAV
FGGAGTIFGPVFGALFLQWVSNWLSTAYTDVAGMFFGIVIVVAVVLMPRGLADLMRNLPRQGWRYFINNMKANKL
>Mature_315_residues
MKYLPALLAIIAAVLALGAPLFASPVMMQLMFDALIIATLAQAWNIIGGFTGYASFGNSVFYGLGAYGAGIAMVQFNLPF
FAGAAIGIVAAVVFAMLIGLPILRLKGHYFAIATLALPQVMAAITANVPVAGENIGLVLPLLNNDTFFYESALILLIIAT
CVVALLTRSRFGFGLIAIRENEEGAAAMGINTTLYKILALMLSGAFCAMAGALQAYKITFLDPEAAYQVQLNVQMIIMAV
FGGAGTIFGPVFGALFLQWVSNWLSTAYTDVAGMFFGIVIVVAVVLMPRGLADLMRNLPRQGWRYFINNMKANKL

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=284, Percent_Identity=25.3521126760563, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 33561; Mature: 33561

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYLPALLAIIAAVLALGAPLFASPVMMQLMFDALIIATLAQAWNIIGGFTGYASFGNSV
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCHH
FYGLGAYGAGIAMVQFNLPFFAGAAIGIVAAVVFAMLIGLPILRLKGHYFAIATLALPQV
HHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHH
MAAITANVPVAGENIGLVLPLLNNDTFFYESALILLIIATCVVALLTRSRFGFGLIAIRE
HHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
NEEGAAAMGINTTLYKILALMLSGAFCAMAGALQAYKITFLDPEAAYQVQLNVQMIIMAV
CCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEEHHHHHHHHH
FGGAGTIFGPVFGALFLQWVSNWLSTAYTDVAGMFFGIVIVVAVVLMPRGLADLMRNLPR
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCH
QGWRYFINNMKANKL
HHHHHHHHCCCCCCC
>Mature Secondary Structure
MKYLPALLAIIAAVLALGAPLFASPVMMQLMFDALIIATLAQAWNIIGGFTGYASFGNSV
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCHH
FYGLGAYGAGIAMVQFNLPFFAGAAIGIVAAVVFAMLIGLPILRLKGHYFAIATLALPQV
HHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHH
MAAITANVPVAGENIGLVLPLLNNDTFFYESALILLIIATCVVALLTRSRFGFGLIAIRE
HHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
NEEGAAAMGINTTLYKILALMLSGAFCAMAGALQAYKITFLDPEAAYQVQLNVQMIIMAV
CCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEEHHHHHHHHH
FGGAGTIFGPVFGALFLQWVSNWLSTAYTDVAGMFFGIVIVVAVVLMPRGLADLMRNLPR
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCH
QGWRYFINNMKANKL
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]