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The map label for this gene is mcbR [H]

Identifier: 225686861

GI number: 225686861

Start: 1079950

End: 1080681

Strand: Reverse

Name: mcbR [H]

Synonym: BMEA_B1102

Alternate gene names: 225686861

Gene position: 1080681-1079950 (Counterclockwise)

Preceding gene: 225686867

Following gene: 225686860

Centisome position: 91.16

GC content: 55.46

Gene sequence:

>732_bases
ATGATGAGCGATATTGAACAGCCATCCCGTGAGAACGACAAGGGTGTTGGACCTGTTGCCCGAGACAGCTTAACCGGGCT
TGTTTATAACAACCTCCGACAAGCGCTGATGGAAGGGCGCTTTTGGCCCGGTCACAGGTTCAAGATTCGGGAACTAGCAG
CGGCAATGAATGTTTCCGAAACGCCGATACGCGAGGCTTTGATGCAGCTGGTGCGGGCGCGCGCGCTGGAAATGCAGGCT
GGCAAATCCATCATCGTCGCTCACATGACTGCCAAACAGTATATTGAGCTTCGCACCGTGCGGCTTTTTCTGGAGGGACT
TGCGGCTGAACATGCCACAACGCGCATTTCCGAAGCCGACATAGATCGGATTGAGGTCGTTCATGAAGAGTTGATCCGCG
CCGAGCAGGAGGGGCGTTGGCCGGACGCCGTCAGGGCTAACTGGCAATTTCACCGCGGTTTGTATGACGCATCCGAACTG
CCCGAAGTGCTGGCCATTCTGGATGACATATGGATGCGGAACGGCCCGCTTTTGAACTTTCATTATCCAGACGCCCCACC
AACCTATCCGAAAGAACACCAGCATCTGGCGGTCTTGAAATATCTTCGGCAACGCCGACCGGATAAGGTCCGGGAGTCAA
TTCAGGCCGATATGATGGAAGGCGGCCAAAACCTCGTGCGCCTTTTGGAAAAATCCGGCGGTACTCGCTTCATGCCGCAG
CGCGCAGAATGA

Upstream 100 bases:

>100_bases
TCTCCTGTAATCCGCGCAGAAGATCGCCTTTTTAGGTTACGGTGGGTGGTTACCTTGTCTATATGATATGCGAAAATTAA
TTGGCTTGAACTGAGATTGA

Downstream 100 bases:

>100_bases
AAAGCGAAACGGAACCACAGTTTCAGGCCCCTGCTTTCTGGCAGGGGGTAATAATTCTTTGGGAAAACATCAATCTCCTG
CTTGAAGCGATGTTTTGCTG

Product: transcriptional regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MMSDIEQPSRENDKGVGPVARDSLTGLVYNNLRQALMEGRFWPGHRFKIRELAAAMNVSETPIREALMQLVRARALEMQA
GKSIIVAHMTAKQYIELRTVRLFLEGLAAEHATTRISEADIDRIEVVHEELIRAEQEGRWPDAVRANWQFHRGLYDASEL
PEVLAILDDIWMRNGPLLNFHYPDAPPTYPKEHQHLAVLKYLRQRRPDKVRESIQADMMEGGQNLVRLLEKSGGTRFMPQ
RAE

Sequences:

>Translated_243_residues
MMSDIEQPSRENDKGVGPVARDSLTGLVYNNLRQALMEGRFWPGHRFKIRELAAAMNVSETPIREALMQLVRARALEMQA
GKSIIVAHMTAKQYIELRTVRLFLEGLAAEHATTRISEADIDRIEVVHEELIRAEQEGRWPDAVRANWQFHRGLYDASEL
PEVLAILDDIWMRNGPLLNFHYPDAPPTYPKEHQHLAVLKYLRQRRPDKVRESIQADMMEGGQNLVRLLEKSGGTRFMPQ
RAE
>Mature_243_residues
MMSDIEQPSRENDKGVGPVARDSLTGLVYNNLRQALMEGRFWPGHRFKIRELAAAMNVSETPIREALMQLVRARALEMQA
GKSIIVAHMTAKQYIELRTVRLFLEGLAAEHATTRISEADIDRIEVVHEELIRAEQEGRWPDAVRANWQFHRGLYDASEL
PEVLAILDDIWMRNGPLLNFHYPDAPPTYPKEHQHLAVLKYLRQRRPDKVRESIQADMMEGGQNLVRLLEKSGGTRFMPQ
RAE

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: COG1802

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=220, Percent_Identity=28.6363636363636, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 27944; Mature: 27944

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSDIEQPSRENDKGVGPVARDSLTGLVYNNLRQALMEGRFWPGHRFKIRELAAAMNVSE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCC
TPIREALMQLVRARALEMQAGKSIIVAHMTAKQYIELRTVRLFLEGLAAEHATTRISEAD
CHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDRIEVVHEELIRAEQEGRWPDAVRANWQFHRGLYDASELPEVLAILDDIWMRNGPLLNF
HHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEE
HYPDAPPTYPKEHQHLAVLKYLRQRRPDKVRESIQADMMEGGQNLVRLLEKSGGTRFMPQ
ECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RAE
CCC
>Mature Secondary Structure
MMSDIEQPSRENDKGVGPVARDSLTGLVYNNLRQALMEGRFWPGHRFKIRELAAAMNVSE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCC
TPIREALMQLVRARALEMQAGKSIIVAHMTAKQYIELRTVRLFLEGLAAEHATTRISEAD
CHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDRIEVVHEELIRAEQEGRWPDAVRANWQFHRGLYDASELPEVLAILDDIWMRNGPLLNF
HHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEE
HYPDAPPTYPKEHQHLAVLKYLRQRRPDKVRESIQADMMEGGQNLVRLLEKSGGTRFMPQ
ECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RAE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]