Click here to switch to the map view.

The map label for this gene is gsiB [H]

Identifier: 225686852

GI number: 225686852

Start: 1069212

End: 1070831

Strand: Direct

Name: gsiB [H]

Synonym: BMEA_B1092

Alternate gene names: 225686852

Gene position: 1069212-1070831 (Clockwise)

Preceding gene: 225686851

Following gene: 225686855

Centisome position: 90.19

GC content: 53.02

Gene sequence:

>1620_bases
ATGCTATTCAAGACAACGGGAACAAGGTCTGGATCATTGAAAACATTGCGAAGCCTGATGATGGCGGCAACCTTGCTGAC
GGCATTCTCGGGGATTGCCTCGGCACAAAGTGCCAATTCACTGGTTATCGCGCGAAATATGGATATCAACTCGCTCGATC
CGCACCGGACCTTCTGCGATACCTGCCAGATCTACGATTCTGCCGTCTATGAGGGTTTGCTTTCGCTGGATAAGGACAAC
AAGGTTGTGCCAGTGCTTGCCGAAAGCTATGCAGCCAATGGCGACCAGACCGAGTTTACGTTCAAAATCAATCCAAAGGC
CGTATTCTCGGATGGTTCACGGGTGGAAGCAAAAGACGTCAAATGGTCGTGGGAACGGCTTAAAAACGTCAAGGGCAGCC
CGTCGTTCCTGGCCGATACTATTGCTTCGATAGAAACGCCCGACGAAGCAACAGTCGTGGTCAAAACCCGGGCACCGAAT
TCTGAATTCTTCAATGTTGTAGCCTCGCCTTATTTCGGTATCGTGAACAGCGATGTGGCCACGACCGAGGCTAAGGCGGA
AAATGGGGCGGACGCAGCGGAGAAAGATAATGCGGAAGCGTGGTTTCTCGCGCATTCGGCTGGCAGCGGTCCCTTCGTCC
TCAGCGCTTATGAGCCAAATTCTGAACTGCGTCTAAAGCGAAACGAAAAATACTGGCGGGAAGCGCCGAAGGTGGATGAG
GTCATCTTCCGCCAAGTGAAGGACGCTGTGGCACAGGCACAAATGCTGGAGAATGGCACTGCAGACATCGCGATGCAGAT
TGATCCCGAGACGGCCAAGACATTCCGTAACAAGGACATCAAGGTCGATTCCATCCATTCCTACAACTTCATCTATCTGG
CGCTTTCGCCGGGTGCAAAATCGAACGCCGTGCCGCTGACTGCAGACATTCGAGAGGCCATTTCTATTGCGATCGACCGT
GAATCGCTGCTCGACTTCATGCTTGGAGACAAAGGTCAGTTGATCGGAACGGCAATTCCGATAGGTTTTCCGGGCGGAAC
GGGGCATAAAATTCCCGAATATAATCCCGAAAAAGCCAAGGAGCTGCTTGCCAAGGCCGGTCATCCTAATGGTTTCACGC
TGGAAGCCGTTTATCCGAACCTGAACGTCTATGGCGTAGATTTCACCCAGATGATGCAAAAGATCCAGCAGGATCTTTCT
CAGATCAATGTCAAGGTCGAGCTGCAGCCGGTGCCGTTTGCAAGCTGGCGTGAAAAGGCTAATGGCGACGGAATCCCGAT
GACAGCCGTTTATTACGCGCCGGATTTCTTCGGCACATCCCAGTATGTGGATGCATTTGGATTGGCGAAGGGCAGTCTCT
GGGCAAAACGCGCCGGGGAGGCTCGCGATCCATCATTCGTAAAGACTGAAATTCCGCAGATCATGGCAGATGCCCTCAGA
GCACCGCTAGATGAGGCGGACAAGGTCTGGTTCAAGGCGGGCGAGGCCATGGCCGCGTCGAATGTCATCATTCCGATGCT
GAGCCCAGACGTTATTCTGGCATATAACAACAGGGTGAACGGCGTGCGCTATTCCGCCTGCTGCAATCTGCCATTGCAGG
AGCTTTCCGTATCCAGATAG

Upstream 100 bases:

>100_bases
TAAACAAGGCCGCCCTCGGATCGGCCGACAAGGTCCGGTCGGCCCTTGAGCAGATTACGCATTCCGCATGACAAAAAACG
TAAAATACGAGGAGGACAAA

Downstream 100 bases:

>100_bases
GCATGACGGGAACCTGTTTTTGAAAAGAAAAAGCCACCGATCCCGCATCGGTGGCTTTTTGCTATGGCAGGCTTTTGTGT
CGCTCAGCCTGCCGCGGAGG

Product: glutathione-binding protein GsiB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MLFKTTGTRSGSLKTLRSLMMAATLLTAFSGIASAQSANSLVIARNMDINSLDPHRTFCDTCQIYDSAVYEGLLSLDKDN
KVVPVLAESYAANGDQTEFTFKINPKAVFSDGSRVEAKDVKWSWERLKNVKGSPSFLADTIASIETPDEATVVVKTRAPN
SEFFNVVASPYFGIVNSDVATTEAKAENGADAAEKDNAEAWFLAHSAGSGPFVLSAYEPNSELRLKRNEKYWREAPKVDE
VIFRQVKDAVAQAQMLENGTADIAMQIDPETAKTFRNKDIKVDSIHSYNFIYLALSPGAKSNAVPLTADIREAISIAIDR
ESLLDFMLGDKGQLIGTAIPIGFPGGTGHKIPEYNPEKAKELLAKAGHPNGFTLEAVYPNLNVYGVDFTQMMQKIQQDLS
QINVKVELQPVPFASWREKANGDGIPMTAVYYAPDFFGTSQYVDAFGLAKGSLWAKRAGEARDPSFVKTEIPQIMADALR
APLDEADKVWFKAGEAMAASNVIIPMLSPDVILAYNNRVNGVRYSACCNLPLQELSVSR

Sequences:

>Translated_539_residues
MLFKTTGTRSGSLKTLRSLMMAATLLTAFSGIASAQSANSLVIARNMDINSLDPHRTFCDTCQIYDSAVYEGLLSLDKDN
KVVPVLAESYAANGDQTEFTFKINPKAVFSDGSRVEAKDVKWSWERLKNVKGSPSFLADTIASIETPDEATVVVKTRAPN
SEFFNVVASPYFGIVNSDVATTEAKAENGADAAEKDNAEAWFLAHSAGSGPFVLSAYEPNSELRLKRNEKYWREAPKVDE
VIFRQVKDAVAQAQMLENGTADIAMQIDPETAKTFRNKDIKVDSIHSYNFIYLALSPGAKSNAVPLTADIREAISIAIDR
ESLLDFMLGDKGQLIGTAIPIGFPGGTGHKIPEYNPEKAKELLAKAGHPNGFTLEAVYPNLNVYGVDFTQMMQKIQQDLS
QINVKVELQPVPFASWREKANGDGIPMTAVYYAPDFFGTSQYVDAFGLAKGSLWAKRAGEARDPSFVKTEIPQIMADALR
APLDEADKVWFKAGEAMAASNVIIPMLSPDVILAYNNRVNGVRYSACCNLPLQELSVSR
>Mature_539_residues
MLFKTTGTRSGSLKTLRSLMMAATLLTAFSGIASAQSANSLVIARNMDINSLDPHRTFCDTCQIYDSAVYEGLLSLDKDN
KVVPVLAESYAANGDQTEFTFKINPKAVFSDGSRVEAKDVKWSWERLKNVKGSPSFLADTIASIETPDEATVVVKTRAPN
SEFFNVVASPYFGIVNSDVATTEAKAENGADAAEKDNAEAWFLAHSAGSGPFVLSAYEPNSELRLKRNEKYWREAPKVDE
VIFRQVKDAVAQAQMLENGTADIAMQIDPETAKTFRNKDIKVDSIHSYNFIYLALSPGAKSNAVPLTADIREAISIAIDR
ESLLDFMLGDKGQLIGTAIPIGFPGGTGHKIPEYNPEKAKELLAKAGHPNGFTLEAVYPNLNVYGVDFTQMMQKIQQDLS
QINVKVELQPVPFASWREKANGDGIPMTAVYYAPDFFGTSQYVDAFGLAKGSLWAKRAGEARDPSFVKTEIPQIMADALR
APLDEADKVWFKAGEAMAASNVIIPMLSPDVILAYNNRVNGVRYSACCNLPLQELSVSR

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=396, Percent_Identity=28.7878787878788, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1787762, Length=433, Percent_Identity=26.0969976905312, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1789887, Length=416, Percent_Identity=25.7211538461538, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789966, Length=446, Percent_Identity=23.3183856502242, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787551, Length=431, Percent_Identity=22.737819025522, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1787495, Length=394, Percent_Identity=24.6192893401015, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58876; Mature: 58876

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFKTTGTRSGSLKTLRSLMMAATLLTAFSGIASAQSANSLVIARNMDINSLDPHRTFCD
CCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHH
TCQIYDSAVYEGLLSLDKDNKVVPVLAESYAANGDQTEFTFKINPKAVFSDGSRVEAKDV
HHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCCCEEEEEEECCEEEECCCCEEECCCC
KWSWERLKNVKGSPSFLADTIASIETPDEATVVVKTRAPNSEFFNVVASPYFGIVNSDVA
CCHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCEECCCCC
TTEAKAENGADAAEKDNAEAWFLAHSAGSGPFVLSAYEPNSELRLKRNEKYWREAPKVDE
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCEEEECCCHHHHHCCCHHH
VIFRQVKDAVAQAQMLENGTADIAMQIDPETAKTFRNKDIKVDSIHSYNFIYLALSPGAK
HHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHCCCCCEEEEEECCEEEEEEECCCCC
SNAVPLTADIREAISIAIDRESLLDFMLGDKGQLIGTAIPIGFPGGTGHKIPEYNPEKAK
CCCCEEHHHHHHHHHEEECHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHH
ELLAKAGHPNGFTLEAVYPNLNVYGVDFTQMMQKIQQDLSQINVKVELQPVPFASWREKA
HHHHHCCCCCCEEEEEECCCCEEEECCHHHHHHHHHHHHHHCEEEEEEECCCCHHHHHCC
NGDGIPMTAVYYAPDFFGTSQYVDAFGLAKGSLWAKRAGEARDPSFVKTEIPQIMADALR
CCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHH
APLDEADKVWFKAGEAMAASNVIIPMLSPDVILAYNNRVNGVRYSACCNLPLQELSVSR
CCCCHHHHHHHHCCCHHHCCCEEEEECCCCEEEEECCCCCCEEEEEECCCCHHHHCCCC
>Mature Secondary Structure
MLFKTTGTRSGSLKTLRSLMMAATLLTAFSGIASAQSANSLVIARNMDINSLDPHRTFCD
CCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHH
TCQIYDSAVYEGLLSLDKDNKVVPVLAESYAANGDQTEFTFKINPKAVFSDGSRVEAKDV
HHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCCCEEEEEEECCEEEECCCCEEECCCC
KWSWERLKNVKGSPSFLADTIASIETPDEATVVVKTRAPNSEFFNVVASPYFGIVNSDVA
CCHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCEECCCCC
TTEAKAENGADAAEKDNAEAWFLAHSAGSGPFVLSAYEPNSELRLKRNEKYWREAPKVDE
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCEEEECCCHHHHHCCCHHH
VIFRQVKDAVAQAQMLENGTADIAMQIDPETAKTFRNKDIKVDSIHSYNFIYLALSPGAK
HHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHCCCCCEEEEEECCEEEEEEECCCCC
SNAVPLTADIREAISIAIDRESLLDFMLGDKGQLIGTAIPIGFPGGTGHKIPEYNPEKAK
CCCCEEHHHHHHHHHEEECHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHH
ELLAKAGHPNGFTLEAVYPNLNVYGVDFTQMMQKIQQDLSQINVKVELQPVPFASWREKA
HHHHHCCCCCCEEEEEECCCCEEEECCHHHHHHHHHHHHHHCEEEEEEECCCCHHHHHCC
NGDGIPMTAVYYAPDFFGTSQYVDAFGLAKGSLWAKRAGEARDPSFVKTEIPQIMADALR
CCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHH
APLDEADKVWFKAGEAMAASNVIIPMLSPDVILAYNNRVNGVRYSACCNLPLQELSVSR
CCCCHHHHHHHHCCCHHHCCCEEEEECCCCEEEEECCCCCCEEEEEECCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA