Click here to switch to the map view.

The map label for this gene is dapE [H]

Identifier: 225686785

GI number: 225686785

Start: 996299

End: 997486

Strand: Direct

Name: dapE [H]

Synonym: BMEA_B1016

Alternate gene names: 225686785

Gene position: 996299-997486 (Clockwise)

Preceding gene: 225686783

Following gene: 225686786

Centisome position: 84.04

GC content: 58.84

Gene sequence:

>1188_bases
ATGACCCTACCTGTCAATCCCGTCGATAATCTTGCCGCCCTCATCCGCTGCCCATCCGTCACACCTGCCGAAGGGGGGGC
GCTGACTGCACTGGAAAAAATGCTGAAGCTCATGGGCTTTTCAGCAAACCGCCCCGTTTTCAGCGATGACAACACGCCCG
ATATTGAAAATCTCTATGCCCGCAAATCCGGCAATGGGCCACATCTGATGTTCGCAGGCCACACCGATGTCGTGCCGCCC
GGCGATGAAAAGGACTGGAAGCACCCGCCCTTTGCCGCAGCAATCGAGGACGGCGTGATGTATGGCCGTGGCGCGGTGGA
CATGAAAGGCGGCATCGCCTGTTTCGTGGCCGCGGTTGCCCGCCACATCGAAAAGCATGGCAACATCAAGGGCAGCATTT
CCTTTCTCATCACCGGCGATGAGGAAGGCCCGGCCGTCAACGGCACGGTGAAACTGCTCGAATGGGCAAAACAACGCGGC
GAAAGCTGGGATGCATCGATCGTCGGTGAACCAACCAACCCGAACGCGCTTGGCGACATGATCAAGATCGGGCGGCGCGG
TTCGCTCTCCGGCACGATTACCGTTCATGGCGTGCAGGGCCATGCCGCCTATCCGCATCTGGCTGAAAATCCGGTCCGTG
GCATCGTCACGCTGGTTGACAGCCTGCTTTACCCCGCCTTCGATGAAGGCACGGCAAATTTCCAGGCCAGCAATCTGGAA
GTCACCACCATTGATGTGGGCAACAAGGCCACCAATGTCATCCCGAACAAGGCGACCGCCTCGTTCAACATCCGCTTCAA
TGACACATGGACGGCCGAAAGCCTTCAAGCGGAAATCATCTCCCGGCTGGAAAGGGCCGCGCGCGACAACCGCCTGCGCC
AGGGGCGCGAAACGCCGATCAAATACGAACTGACCTGGCGTGAGCGGCCAAGCCATGTCTTCCTCACCCATGATGAAAAA
CTGATCGGCACGCTCACCGCATCGGTAGAGGCCGTAACCGGCAAACGCCCGGAACTGTCCACTTCAGGCGGCACGTCCGA
TGCCCGCTTCATCAAGGATTATTGCCCGGTGGTGGAGTTTGGCCTTACGGGCCAGACCATGCATATGGTTGATGAGCGCG
TGGCCCTTGCCGACCTCGAAGGCCTGACGCAGATTTATGAACGCTTCATCGCGGATTTCTTTGGGTAA

Upstream 100 bases:

>100_bases
GAAAATTGCGGGTTACACGCCAGACGGGATTGTGGTTTCCTCCGGCACTTTCCCGATCTAATCTTACGGCAGTCGATTCC
CTTTACAGAAGCCGCACCCC

Downstream 100 bases:

>100_bases
AGCTTGGCGGCGGGAAACTGCACGGCTTTCGGGAAATTAACGATACAAAAATTGGGAACCTCATGCCAAGTCTGGATGAT
ATTTTCAGATATTTCCGGGG

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MTLPVNPVDNLAALIRCPSVTPAEGGALTALEKMLKLMGFSANRPVFSDDNTPDIENLYARKSGNGPHLMFAGHTDVVPP
GDEKDWKHPPFAAAIEDGVMYGRGAVDMKGGIACFVAAVARHIEKHGNIKGSISFLITGDEEGPAVNGTVKLLEWAKQRG
ESWDASIVGEPTNPNALGDMIKIGRRGSLSGTITVHGVQGHAAYPHLAENPVRGIVTLVDSLLYPAFDEGTANFQASNLE
VTTIDVGNKATNVIPNKATASFNIRFNDTWTAESLQAEIISRLERAARDNRLRQGRETPIKYELTWRERPSHVFLTHDEK
LIGTLTASVEAVTGKRPELSTSGGTSDARFIKDYCPVVEFGLTGQTMHMVDERVALADLEGLTQIYERFIADFFG

Sequences:

>Translated_395_residues
MTLPVNPVDNLAALIRCPSVTPAEGGALTALEKMLKLMGFSANRPVFSDDNTPDIENLYARKSGNGPHLMFAGHTDVVPP
GDEKDWKHPPFAAAIEDGVMYGRGAVDMKGGIACFVAAVARHIEKHGNIKGSISFLITGDEEGPAVNGTVKLLEWAKQRG
ESWDASIVGEPTNPNALGDMIKIGRRGSLSGTITVHGVQGHAAYPHLAENPVRGIVTLVDSLLYPAFDEGTANFQASNLE
VTTIDVGNKATNVIPNKATASFNIRFNDTWTAESLQAEIISRLERAARDNRLRQGRETPIKYELTWRERPSHVFLTHDEK
LIGTLTASVEAVTGKRPELSTSGGTSDARFIKDYCPVVEFGLTGQTMHMVDERVALADLEGLTQIYERFIADFFG
>Mature_394_residues
TLPVNPVDNLAALIRCPSVTPAEGGALTALEKMLKLMGFSANRPVFSDDNTPDIENLYARKSGNGPHLMFAGHTDVVPPG
DEKDWKHPPFAAAIEDGVMYGRGAVDMKGGIACFVAAVARHIEKHGNIKGSISFLITGDEEGPAVNGTVKLLEWAKQRGE
SWDASIVGEPTNPNALGDMIKIGRRGSLSGTITVHGVQGHAAYPHLAENPVRGIVTLVDSLLYPAFDEGTANFQASNLEV
TTIDVGNKATNVIPNKATASFNIRFNDTWTAESLQAEIISRLERAARDNRLRQGRETPIKYELTWRERPSHVFLTHDEKL
IGTLTASVEAVTGKRPELSTSGGTSDARFIKDYCPVVEFGLTGQTMHMVDERVALADLEGLTQIYERFIADFFG

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=391, Percent_Identity=39.8976982097187, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI1790395, Length=408, Percent_Identity=26.7156862745098, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 42838; Mature: 42707

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00758 ARGE_DAPE_CPG2_1 ; PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPVNPVDNLAALIRCPSVTPAEGGALTALEKMLKLMGFSANRPVFSDDNTPDIENLYA
CCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
RKSGNGPHLMFAGHTDVVPPGDEKDWKHPPFAAAIEDGVMYGRGAVDMKGGIACFVAAVA
HCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCEEECCCCCCCCCCHHHHHHHHH
RHIEKHGNIKGSISFLITGDEEGPAVNGTVKLLEWAKQRGESWDASIVGEPTNPNALGDM
HHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHH
IKIGRRGSLSGTITVHGVQGHAAYPHLAENPVRGIVTLVDSLLYPAFDEGTANFQASNLE
HHHCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCEE
VTTIDVGNKATNVIPNKATASFNIRFNDTWTAESLQAEIISRLERAARDNRLRQGRETPI
EEEEECCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
KYELTWRERPSHVFLTHDEKLIGTLTASVEAVTGKRPELSTSGGTSDARFIKDYCPVVEF
EEEEEECCCCCEEEEECCCCEEHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCEEE
GLTGQTMHMVDERVALADLEGLTQIYERFIADFFG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLPVNPVDNLAALIRCPSVTPAEGGALTALEKMLKLMGFSANRPVFSDDNTPDIENLYA
CCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
RKSGNGPHLMFAGHTDVVPPGDEKDWKHPPFAAAIEDGVMYGRGAVDMKGGIACFVAAVA
HCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCEEECCCCCCCCCCHHHHHHHHH
RHIEKHGNIKGSISFLITGDEEGPAVNGTVKLLEWAKQRGESWDASIVGEPTNPNALGDM
HHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHH
IKIGRRGSLSGTITVHGVQGHAAYPHLAENPVRGIVTLVDSLLYPAFDEGTANFQASNLE
HHHCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCEE
VTTIDVGNKATNVIPNKATASFNIRFNDTWTAESLQAEIISRLERAARDNRLRQGRETPI
EEEEECCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
KYELTWRERPSHVFLTHDEKLIGTLTASVEAVTGKRPELSTSGGTSDARFIKDYCPVVEF
EEEEEECCCCCEEEEECCCCEEHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCEEE
GLTGQTMHMVDERVALADLEGLTQIYERFIADFFG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA