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The map label for this gene is tpiA [H]

Identifier: 225686631

GI number: 225686631

Start: 833726

End: 834496

Strand: Direct

Name: tpiA [H]

Synonym: BMEA_B0848

Alternate gene names: 225686631

Gene position: 833726-834496 (Clockwise)

Preceding gene: 225686630

Following gene: 225686632

Centisome position: 70.33

GC content: 59.92

Gene sequence:

>771_bases
ATGACCAAATTCTGGATTGGCACAAGCTGGAAAATGAACAAGACGCTGGCCGAAGCCCGCCTTTTCGCGGAAGCGCTGAA
AGCCGCCGATGCAGGCCGCTCGCCAGACATCCAGCGTTTTGTCATCCCGCCTTTCACCGCCGTGCGTGAGGTGAAGGAAA
TCCTCTCCGGCACATCCGTGAAGGTCGGCGCGCAGAACATGCACTGGGCCGATCAGGGTACATGGACCGGCGAAATCTCC
CCGCTGATGCTGAAAGATTGCAATCTCGACATTGTTGAACTGGGCCATTCGGAGCGCCGCGAACATTTCGGCGAGACCAA
CGAGACGGTCGGCTTGAAAGTGGAAGCCGCCGTGCGCCACGGCCTGATCCCGCTCATCTGCATCGGTGAAACGCTGGAAG
ACCGTGAAAGCGGACGCGCAGCCGCGGTGCTTGAAGAAGAAGTGCGCGGCGCGCTTTCCAAGCTTTCCGAGGCGCAGAAG
CAGGCCGAAATCCTCTTCGCCTATGAGCCAGTCTGGGCCATCGGCGAAAACGGCATTCCCGCTTCCGCCGACTATGCCGA
TGCGCGACAGGCCGAAATCATCGCGGTGGCACAAAGCGTGCTCGCTCGCCGCGTGCCGTGCCTTTATGGCGGCTCGGTCA
ATCCGGGCAATTGCGAAGAACTGATCGCCTGCCCGCATATCGATGGGCTTTTCATCGGCCGTTCGGCGTGGAACGTCGAA
GGCTATCTCGACATTCTGGCCAGGTGCGCCACCAAGGTTCAAGCGAATTAA

Upstream 100 bases:

>100_bases
CGCGATCATCACCGATGCATCCCCCGGCACGGCGGTTTTGAACGGACTGAAACAGGCCGGGATCAAACTGACAATAGCAG
CAGACTAGGAACAGTTACGC

Downstream 100 bases:

>100_bases
GGAGTAAAATCATGAAAGTAGCAGTTGCAGGCGACAGCGCCGGCGAAGGCCTGGCCAAGGTTCTCGCCGATCACCTCAAG
GACCGCTTCGAGGTTTCGGA

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM 2; Triose-phosphate isomerase 2 [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MTKFWIGTSWKMNKTLAEARLFAEALKAADAGRSPDIQRFVIPPFTAVREVKEILSGTSVKVGAQNMHWADQGTWTGEIS
PLMLKDCNLDIVELGHSERREHFGETNETVGLKVEAAVRHGLIPLICIGETLEDRESGRAAAVLEEEVRGALSKLSEAQK
QAEILFAYEPVWAIGENGIPASADYADARQAEIIAVAQSVLARRVPCLYGGSVNPGNCEELIACPHIDGLFIGRSAWNVE
GYLDILARCATKVQAN

Sequences:

>Translated_256_residues
MTKFWIGTSWKMNKTLAEARLFAEALKAADAGRSPDIQRFVIPPFTAVREVKEILSGTSVKVGAQNMHWADQGTWTGEIS
PLMLKDCNLDIVELGHSERREHFGETNETVGLKVEAAVRHGLIPLICIGETLEDRESGRAAAVLEEEVRGALSKLSEAQK
QAEILFAYEPVWAIGENGIPASADYADARQAEIIAVAQSVLARRVPCLYGGSVNPGNCEELIACPHIDGLFIGRSAWNVE
GYLDILARCATKVQAN
>Mature_255_residues
TKFWIGTSWKMNKTLAEARLFAEALKAADAGRSPDIQRFVIPPFTAVREVKEILSGTSVKVGAQNMHWADQGTWTGEISP
LMLKDCNLDIVELGHSERREHFGETNETVGLKVEAAVRHGLIPLICIGETLEDRESGRAAAVLEEEVRGALSKLSEAQKQ
AEILFAYEPVWAIGENGIPASADYADARQAEIIAVAQSVLARRVPCLYGGSVNPGNCEELIACPHIDGLFIGRSAWNVEG
YLDILARCATKVQAN

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4507645, Length=247, Percent_Identity=34.412955465587, Blast_Score=138, Evalue=4e-33,
Organism=Homo sapiens, GI226529917, Length=247, Percent_Identity=34.412955465587, Blast_Score=138, Evalue=5e-33,
Organism=Escherichia coli, GI1790353, Length=217, Percent_Identity=33.6405529953917, Blast_Score=121, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17536593, Length=235, Percent_Identity=37.4468085106383, Blast_Score=126, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6320255, Length=247, Percent_Identity=31.5789473684211, Blast_Score=118, Evalue=8e-28,
Organism=Drosophila melanogaster, GI28572008, Length=251, Percent_Identity=35.4581673306773, Blast_Score=128, Evalue=3e-30,
Organism=Drosophila melanogaster, GI28572006, Length=251, Percent_Identity=35.4581673306773, Blast_Score=128, Evalue=3e-30,
Organism=Drosophila melanogaster, GI28572004, Length=249, Percent_Identity=34.9397590361446, Blast_Score=128, Evalue=4e-30,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861 [H]

Pfam domain/function: PF00121 TIM [H]

EC number: =5.3.1.1 [H]

Molecular weight: Translated: 27901; Mature: 27770

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00889 CNMP_BINDING_2 ; PS00171 TIM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKFWIGTSWKMNKTLAEARLFAEALKAADAGRSPDIQRFVIPPFTAVREVKEILSGTSV
CCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHCCCCE
KVGAQNMHWADQGTWTGEISPLMLKDCNLDIVELGHSERREHFGETNETVGLKVEAAVRH
EECCCCCCCCCCCCCCCCCCCEEEECCCEEHEECCCHHHHHHCCCCCCEEEEEEHHHHHH
GLIPLICIGETLEDRESGRAAAVLEEEVRGALSKLSEAQKQAEILFAYEPVWAIGENGIP
CCEEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEECCCCCC
ASADYADARQAEIIAVAQSVLARRVPCLYGGSVNPGNCEELIACPHIDGLFIGRSAWNVE
CCCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCCCCCHHHEEECCCCCCEEECCCCCCHH
GYLDILARCATKVQAN
HHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKFWIGTSWKMNKTLAEARLFAEALKAADAGRSPDIQRFVIPPFTAVREVKEILSGTSV
CEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHCCCCE
KVGAQNMHWADQGTWTGEISPLMLKDCNLDIVELGHSERREHFGETNETVGLKVEAAVRH
EECCCCCCCCCCCCCCCCCCCEEEECCCEEHEECCCHHHHHHCCCCCCEEEEEEHHHHHH
GLIPLICIGETLEDRESGRAAAVLEEEVRGALSKLSEAQKQAEILFAYEPVWAIGENGIP
CCEEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEECCCCCC
ASADYADARQAEIIAVAQSVLARRVPCLYGGSVNPGNCEELIACPHIDGLFIGRSAWNVE
CCCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCCCCCHHHEEECCCCCCEEECCCCCCHH
GYLDILARCATKVQAN
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10708387 [H]