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The map label for this gene is 225686531

Identifier: 225686531

GI number: 225686531

Start: 722641

End: 724014

Strand: Direct

Name: 225686531

Synonym: BMEA_B0741

Alternate gene names: NA

Gene position: 722641-724014 (Clockwise)

Preceding gene: 225686530

Following gene: 225686533

Centisome position: 60.96

GC content: 58.81

Gene sequence:

>1374_bases
ATGGCGCGCGATACGACAGACGAAACGGCACTGACGAGCGTTGAGGAACTCGCGACCTATCTGGCCGGCGGCTGCAAACC
GAAAGACGCCTGGCGCATTGGCACCGAGCACGAAAAATTTCCCTTCTATACGGCCAATAACAGCCCCGTGCCCTATGGCG
GTCCGCGCGGCATCAAGGCCATTCTGGAAGGCATGCAGGCCAGACTCGGCTGGGAACCGATCATGGACGAAGGCAACATT
ATCGGCCTCGTGGAGCCAACGGGACAAGGCGCCATTTCACTGGAGCCGGGCGGACAGTTCGAGCTTTCCGGCGCGCCGCT
TTCCACCATTCACCAGACCTGCCGCGAAGTGAATGCGCATCTAAGCCAGGTGCGCGAAATCGCCGAGCCTCTGGGTATCC
GTTTCCTTGGCGTTGGCGGCAGCCCGAAATGGACGCTGGCCGAAACGCCCGCCATGCCGAAATCGCGTTACAAGATCATG
ACCAACTACATGCCGAAGGTGGGGCATGAAGGGCTGGACATGATGTACCGCACCTCGACCATTCAGGTTAATCTCGACTT
CAGCTCGGAACAGGACATGCGCCGCAAGATGCAGGTTTCCATGAAGCTGCAATCGGTCGCGACCGCGCTTTTCGCAAGCT
CGCCCTTCACCGAAGGAAAGCCGAACGGCCTTCTCTCGTGGCGCTCCAGCATCTGGCGCGACACGGACAACCAGCGTTCG
GGCGTGCTGCCCTTCGTCTTTTCCGAGAATTTCGGCTTTGCGGATTATGTGAACTGGGCGCTCGACGTGCCAATGTATTT
CATCCTGCGTGACGGCCATTATCATGATTGCACGCATGTTAGCTTCCGCCAGTTCATGAACGGCGCATTGAAGGGCGAAG
TCAGCGATCCGGTTCCCAATATGGGCGACTGGACCAACCATCTTTCAACGCTTTTTCCCGAAGTGCGCCTGAAGCGCTTC
CTCGAAATGCGCGGCGCCGATGGCGGCCCGTGGCGGCGCATCTGCGCCCTGCCTGCCTATTGGGTGGGGCTGCTTTATGA
TGAAGAGGCGCTGGCCGCCGCCGAACTTCTGACGAAAGACTGGACCTATGAAGAAGTGCTGGCACTTCGCAACGAGGTGC
CCACCAAGGCGCTCAAGACCGATTTTCACGGCAAGCCGCTGGAACTGATTGCCCGCGAAACACTGAAAATCTCCCGCCTC
GGCCTTCTGAACCGCAAGAAGCTCAACAGTGACGGTTATGACGAGACGCATTTCCTTGCCCCGCTGGAGGAAATCGTCGC
CGCAGCTACAACCGATGCCGAGCGCATGTTGAACGCCTATCACAGTGTCTGGGCCGGCTCGGTGGATCCGATCTTCCTCG
AATATGCCTATTGA

Upstream 100 bases:

>100_bases
AAGTTTTCCAAATTTCGAGGTTCAATGGAATTGACTTGCGTGATTTGACAAATTTGTCCAATCACGCTGACAAACTGAAA
TACGACCAGGGATAAGTTGC

Downstream 100 bases:

>100_bases
AGAAGCGTTCCGATGTCACAAAATGGAAAAGGGCGCGATGATCGCGCCTTTTTTCAGCTTGAGACTTACTGGCCTTTGGT
CGGATCGGGGATTGTGAGCC

Product: glutamate--cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Gamma-Glutamylcysteine Synthetase; Glutamate/Cysteine Ligase Family Protein; Glutamate-Cysteine Ligase GshA; Glutamylcysteine Synthetase; Glutamate-Cysteine Ligase Family 2 Protein; Glutamate-Cysteine Ligase Protein; Glutathione Synthetase; Glutamate-Cysteine Ligase Gsha; Glutamate-Cysteine Ligase Protein; Glutamate-Cysteine Ligase Family Protein; Glutamate Cystein Ligase Protein

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MARDTTDETALTSVEELATYLAGGCKPKDAWRIGTEHEKFPFYTANNSPVPYGGPRGIKAILEGMQARLGWEPIMDEGNI
IGLVEPTGQGAISLEPGGQFELSGAPLSTIHQTCREVNAHLSQVREIAEPLGIRFLGVGGSPKWTLAETPAMPKSRYKIM
TNYMPKVGHEGLDMMYRTSTIQVNLDFSSEQDMRRKMQVSMKLQSVATALFASSPFTEGKPNGLLSWRSSIWRDTDNQRS
GVLPFVFSENFGFADYVNWALDVPMYFILRDGHYHDCTHVSFRQFMNGALKGEVSDPVPNMGDWTNHLSTLFPEVRLKRF
LEMRGADGGPWRRICALPAYWVGLLYDEEALAAAELLTKDWTYEEVLALRNEVPTKALKTDFHGKPLELIARETLKISRL
GLLNRKKLNSDGYDETHFLAPLEEIVAAATTDAERMLNAYHSVWAGSVDPIFLEYAY

Sequences:

>Translated_457_residues
MARDTTDETALTSVEELATYLAGGCKPKDAWRIGTEHEKFPFYTANNSPVPYGGPRGIKAILEGMQARLGWEPIMDEGNI
IGLVEPTGQGAISLEPGGQFELSGAPLSTIHQTCREVNAHLSQVREIAEPLGIRFLGVGGSPKWTLAETPAMPKSRYKIM
TNYMPKVGHEGLDMMYRTSTIQVNLDFSSEQDMRRKMQVSMKLQSVATALFASSPFTEGKPNGLLSWRSSIWRDTDNQRS
GVLPFVFSENFGFADYVNWALDVPMYFILRDGHYHDCTHVSFRQFMNGALKGEVSDPVPNMGDWTNHLSTLFPEVRLKRF
LEMRGADGGPWRRICALPAYWVGLLYDEEALAAAELLTKDWTYEEVLALRNEVPTKALKTDFHGKPLELIARETLKISRL
GLLNRKKLNSDGYDETHFLAPLEEIVAAATTDAERMLNAYHSVWAGSVDPIFLEYAY
>Mature_456_residues
ARDTTDETALTSVEELATYLAGGCKPKDAWRIGTEHEKFPFYTANNSPVPYGGPRGIKAILEGMQARLGWEPIMDEGNII
GLVEPTGQGAISLEPGGQFELSGAPLSTIHQTCREVNAHLSQVREIAEPLGIRFLGVGGSPKWTLAETPAMPKSRYKIMT
NYMPKVGHEGLDMMYRTSTIQVNLDFSSEQDMRRKMQVSMKLQSVATALFASSPFTEGKPNGLLSWRSSIWRDTDNQRSG
VLPFVFSENFGFADYVNWALDVPMYFILRDGHYHDCTHVSFRQFMNGALKGEVSDPVPNMGDWTNHLSTLFPEVRLKRFL
EMRGADGGPWRRICALPAYWVGLLYDEEALAAAELLTKDWTYEEVLALRNEVPTKALKTDFHGKPLELIARETLKISRLG
LLNRKKLNSDGYDETHFLAPLEEIVAAATTDAERMLNAYHSVWAGSVDPIFLEYAY

Specific function: Unknown

COG id: COG3572

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 51199; Mature: 51068

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDTTDETALTSVEELATYLAGGCKPKDAWRIGTEHEKFPFYTANNSPVPYGGPRGIKA
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCEEECCCCCCCCCCCHHHHH
ILEGMQARLGWEPIMDEGNIIGLVEPTGQGAISLEPGGQFELSGAPLSTIHQTCREVNAH
HHHHHHHHCCCCCCCCCCCEEEEECCCCCCEEEECCCCCEEECCCCHHHHHHHHHHHHHH
LSQVREIAEPLGIRFLGVGGSPKWTLAETPAMPKSRYKIMTNYMPKVGHEGLDMMYRTST
HHHHHHHHHHHCEEEEECCCCCCEEECCCCCCCHHHHHHHHHHCHHCCCCCCCEEEEEEE
IQVNLDFSSEQDMRRKMQVSMKLQSVATALFASSPFTEGKPNGLLSWRSSIWRDTDNQRS
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCC
GVLPFVFSENFGFADYVNWALDVPMYFILRDGHYHDCTHVSFRQFMNGALKGEVSDPVPN
CCCEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
MGDWTNHLSTLFPEVRLKRFLEMRGADGGPWRRICALPAYWVGLLYDEEALAAAELLTKD
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCC
WTYEEVLALRNEVPTKALKTDFHGKPLELIARETLKISRLGLLNRKKLNSDGYDETHFLA
CCHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHH
PLEEIVAAATTDAERMLNAYHSVWAGSVDPIFLEYAY
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECC
>Mature Secondary Structure 
ARDTTDETALTSVEELATYLAGGCKPKDAWRIGTEHEKFPFYTANNSPVPYGGPRGIKA
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCEEECCCCCCCCCCCHHHHH
ILEGMQARLGWEPIMDEGNIIGLVEPTGQGAISLEPGGQFELSGAPLSTIHQTCREVNAH
HHHHHHHHCCCCCCCCCCCEEEEECCCCCCEEEECCCCCEEECCCCHHHHHHHHHHHHHH
LSQVREIAEPLGIRFLGVGGSPKWTLAETPAMPKSRYKIMTNYMPKVGHEGLDMMYRTST
HHHHHHHHHHHCEEEEECCCCCCEEECCCCCCCHHHHHHHHHHCHHCCCCCCCEEEEEEE
IQVNLDFSSEQDMRRKMQVSMKLQSVATALFASSPFTEGKPNGLLSWRSSIWRDTDNQRS
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCC
GVLPFVFSENFGFADYVNWALDVPMYFILRDGHYHDCTHVSFRQFMNGALKGEVSDPVPN
CCCEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
MGDWTNHLSTLFPEVRLKRFLEMRGADGGPWRRICALPAYWVGLLYDEEALAAAELLTKD
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCC
WTYEEVLALRNEVPTKALKTDFHGKPLELIARETLKISRLGLLNRKKLNSDGYDETHFLA
CCHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHH
PLEEIVAAATTDAERMLNAYHSVWAGSVDPIFLEYAY
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA