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The map label for this gene is pcaJ [H]

Identifier: 225686417

GI number: 225686417

Start: 598017

End: 598706

Strand: Reverse

Name: pcaJ [H]

Synonym: BMEA_B0610

Alternate gene names: 225686417

Gene position: 598706-598017 (Counterclockwise)

Preceding gene: 225686418

Following gene: 225686416

Centisome position: 50.5

GC content: 57.83

Gene sequence:

>690_bases
ATGACCATCGATACGCTTGAAGACATCAAGCTTTCCAATGCCCAGATTGCCTGGCGCGCGGCGCAGGATATTGCCGACGG
CGCCTATGTGAACCTTGGGATCGGTTTTCCCGAAATGGTTGCCCGCTATCAGCCGGAAGGCCGCGAGGCGATCTTCCACA
CCGAAAACGGCATTCTTGATTTTGGCGAAGCGCCGCCGCCGGGTGAGGAAGACTGGGATCTGATCAATGCGGGCAAGAAA
GCTGTGACGCTGAAGCCGGGAGCGGCCTTCTTCCACCATGCAGACAGTTTTTCCATGGTGCGCGGCGGCCATCTCGATGG
GGCGATCCTCGGCGCCTATCAGGTTGCGCAAAATGGCGATCTTGCCAATTGGCGCGTGGGCGCAAAGGGCGTTCCGGCGG
TTGGCGGCGCGATGGATCTCGTTCATGGCGCAAAACAGGTGGTCGTCATCACCGAACATGTCACCAAAAAGGGGGAACCC
AAGCTGGTGGAACAATGCACTTTCCCGTTGACGGGTGTGAAGTGCATTACGCGCATTTATACGAGCCACGCGGTCGTGGA
TGTTTCCGAAGGCCGTTTTGTGCTGCGTGAAAAGCTTGCCGCGATGACGCTGGAAGAGCTTCAGGCTATGACCGGCGCCA
AGCTGCATATTGAAGGCCCCGTGGCCGATCTTGTCGTTCCTGAATTGTGA

Upstream 100 bases:

>100_bases
CGGAAAATGTCATCACCCCCGGCATTTTCGTGGACCGTGTGGTGGAAATCGCCAACCCGCAGCAGGAAGAAGAGCTTATT
CGGGCAGGAGTGGCCTACAT

Downstream 100 bases:

>100_bases
GAAGCATTATGACTGAAGCCTATATCTGCGACTATATCCGCACGCCGATCGGCCGTTACGGCGGCGCGCTTTCCTCCGTT
CGCGCGGATGATCTGGGTGC

Product: 3-oxoacid CoA-transferase, B subunit

Products: NA

Alternate protein names: Beta-ketoadipate:succinyl-CoA transferase subunit B [H]

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MTIDTLEDIKLSNAQIAWRAAQDIADGAYVNLGIGFPEMVARYQPEGREAIFHTENGILDFGEAPPPGEEDWDLINAGKK
AVTLKPGAAFFHHADSFSMVRGGHLDGAILGAYQVAQNGDLANWRVGAKGVPAVGGAMDLVHGAKQVVVITEHVTKKGEP
KLVEQCTFPLTGVKCITRIYTSHAVVDVSEGRFVLREKLAAMTLEELQAMTGAKLHIEGPVADLVVPEL

Sequences:

>Translated_229_residues
MTIDTLEDIKLSNAQIAWRAAQDIADGAYVNLGIGFPEMVARYQPEGREAIFHTENGILDFGEAPPPGEEDWDLINAGKK
AVTLKPGAAFFHHADSFSMVRGGHLDGAILGAYQVAQNGDLANWRVGAKGVPAVGGAMDLVHGAKQVVVITEHVTKKGEP
KLVEQCTFPLTGVKCITRIYTSHAVVDVSEGRFVLREKLAAMTLEELQAMTGAKLHIEGPVADLVVPEL
>Mature_228_residues
TIDTLEDIKLSNAQIAWRAAQDIADGAYVNLGIGFPEMVARYQPEGREAIFHTENGILDFGEAPPPGEEDWDLINAGKKA
VTLKPGAAFFHHADSFSMVRGGHLDGAILGAYQVAQNGDLANWRVGAKGVPAVGGAMDLVHGAKQVVVITEHVTKKGEPK
LVEQCTFPLTGVKCITRIYTSHAVVDVSEGRFVLREKLAAMTLEELQAMTGAKLHIEGPVADLVVPEL

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI11545841, Length=209, Percent_Identity=44.9760765550239, Blast_Score=184, Evalue=5e-47,
Organism=Homo sapiens, GI4557817, Length=205, Percent_Identity=45.3658536585366, Blast_Score=176, Evalue=1e-44,
Organism=Escherichia coli, GI1788552, Length=203, Percent_Identity=42.3645320197044, Blast_Score=163, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17531517, Length=205, Percent_Identity=45.8536585365854, Blast_Score=178, Evalue=2e-45,
Organism=Drosophila melanogaster, GI21358325, Length=206, Percent_Identity=45.1456310679612, Blast_Score=174, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24655794, Length=206, Percent_Identity=45.1456310679612, Blast_Score=174, Evalue=6e-44,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.6 [H]

Molecular weight: Translated: 24564; Mature: 24433

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIDTLEDIKLSNAQIAWRAAQDIADGAYVNLGIGFPEMVARYQPEGREAIFHTENGILD
CCCCCCCCCCCCCCEEEEEHHHHHCCCCEEEECCCCHHHHHHCCCCCCEEEEECCCCEEE
FGEAPPPGEEDWDLINAGKKAVTLKPGAAFFHHADSFSMVRGGHLDGAILGAYQVAQNGD
CCCCCCCCCCCCHHHHCCCEEEEECCCCCEEECCCCCCEEECCCCCCCEEEEEEHHCCCC
LANWRVGAKGVPAVGGAMDLVHGAKQVVVITEHVTKKGEPKLVEQCTFPLTGVKCITRIY
CCEEEECCCCCCCCCHHHHHHCCCCEEEEEEEHHCCCCCCHHHHHCCCCCHHHHHHHHHH
TSHAVVDVSEGRFVLREKLAAMTLEELQAMTGAKLHIEGPVADLVVPEL
HCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHCCCCC
>Mature Secondary Structure 
TIDTLEDIKLSNAQIAWRAAQDIADGAYVNLGIGFPEMVARYQPEGREAIFHTENGILD
CCCCCCCCCCCCCEEEEEHHHHHCCCCEEEECCCCHHHHHHCCCCCCEEEEECCCCEEE
FGEAPPPGEEDWDLINAGKKAVTLKPGAAFFHHADSFSMVRGGHLDGAILGAYQVAQNGD
CCCCCCCCCCCCHHHHCCCEEEEECCCCCEEECCCCCCEEECCCCCCCEEEEEEHHCCCC
LANWRVGAKGVPAVGGAMDLVHGAKQVVVITEHVTKKGEPKLVEQCTFPLTGVKCITRIY
CCEEEECCCCCCCCCHHHHHHCCCCEEEEEEEHHCCCCCCHHHHHCCCCCHHHHHHHHHH
TSHAVVDVSEGRFVLREKLAAMTLEELQAMTGAKLHIEGPVADLVVPEL
HCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8063101; 8125318 [H]