| Definition | Brucella melitensis ATCC 23457 chromosome chromosome II, complete sequence. |
|---|---|
| Accession | NC_012442 |
| Length | 1,185,518 |
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The map label for this gene is topA [H]
Identifier: 225686390
GI number: 225686390
Start: 569358
End: 571991
Strand: Direct
Name: topA [H]
Synonym: BMEA_B0579
Alternate gene names: 225686390
Gene position: 569358-571991 (Clockwise)
Preceding gene: 225686388
Following gene: 225686391
Centisome position: 48.03
GC content: 59.72
Gene sequence:
>2634_bases ATGAACGTCGTCGTCGTCGAATCGCCTGCCAAGGCCAAAACCATCAACAAATATCTGGGCTCGGACTATAAGGTTCTGGC CTCCTTTGGCCATGTGCGGGACCTGCCAGCCAAGGATGGCTCGGTGCGCCCGGACGAAGATTTTGCAATGTCCTGGGAAG TGGACAGCAATTCCGCCAAGCGCCTGGCGGATATTGTAAAGGCACTGAAGGACAGCGACGGCCTCATTCTCGCAACCGAC CCTGATCGCGAAGGGGAAGCCATTTCCTGGCATGTGCTGGAAGTGCTGCGGCAGAAAAAGGCGCTCAACGGCAAAACGGT GAAGCGCGTGGCCTTCAACGCCATCACCAAAAAGGCCGTTCTGGATGCAATTGCCCATCCACGCGATATTGATGAGCCGC TGGTGGACGCCTATCTCGCCCGCCGTGCACTCGATTATCTGGTCGGCTTCACCCTTTCGCCGGTGCTCTGGCGCAAGCTT CCCGGCGCCCGCTCGGCCGGTCGCGTGCAATCGGTAGCGCTGCGTCTGGTCTGCGACCGCGAAGCGGAAATCGAACGCTT TGTCCGCGAGGAATACTGGAATATCGCAGCCCTTCTCAAGACACCGCGCAATGACAGTTTCACTGCCCGCCTCACCGCGC TTGATGGCAAGAAGCTTGGAAAGCTCGATATCAAGAACGGCGAACAGGCCGGTGCCATCCGCACCATGCTGGAAAGCGCG AACTTCAAGGCTGTTTCCGTCGAGGCAAAGCCTACCAAGCGCAATCCGGGCCCGCCATTCACCACCTCCACCTTGCAGCA GGCCGCCTCGGGGCAATTGGGCTTTTCTGCTTCGCGCACCATGCAGGTGGCGCAGCGCCTTTATGAAGGCGTGGACATTG GCGGCGAAACAGCCGGTCTCATAACCTATATGCGTACCGATGGTGTGCAGATTGCTCCCGAAGCGATCAATGCCGCCCGA GACGCTATCGCCAAGAGTTTCGGCGCGAAATATGTGCCCGAAAAGCCGCGGTATTATTCGAGCAAGGCCAAGAATGCGCA GGAAGCGCACGAAGCAATCCGCCCCACTGATTTTTCGCGTCATCCAAAGGATGTGCGCCGCTTTCTCGATGAGGATCAGG CCCGCCTTTACGAACTGATCTGGAAGCGCGCCATTGCAAGCCAGATGCAGGCCGCCGATATCGAGCGCACCACGGTCGAT ATCGAGGCCACCAACGGTTCACGCGCCGCCATGCTGCGCGCCAATGGCTCGGTGACGAAATTCGACGGCTTCCTTGCCGC CTATATGGATCATCGCGAGGAGGAGGACGACGACGAAGAGAGTGCAAAGCTGCCGGAAATTCGCTCCGGCGAGGCGCTTG CCCGCGAAAAGATCGATGCGACCCAGCATTCGACCGAACCGCCGCCGCGCTATTCGGAAGCAACCCTTATCAAGAAGATG GAAGAGTTGGGCATTGGCCGTCCTTCCACCTATGCCGCAACACTCGCCACGCTGCGCGACCGCGAATATATCATCATCGA CAAGCGCAAGCTGATTCCCGAAGCCAAGGGGCGCCTTGTGACGGCGTTTCTTGAAAGCTTCTTCGAGCGCTATGTGGAAT ATGATTTCACGGCAGACCTGGAGGAAAAGCTTGATCTTATTTCCGATGGCAAATTGTCGTGGAAAGAGATTCTGCGCGAT TTCTGGCGTGCATTCTCCGGCTCCGTCGAGGACATCAAGGAACTGCGCGTCACCAATGTTCTGGATGCGCTGAATGAGGA ACTGGCTCCGCTGGTCTTTCCTGCGCGCGAGGATGGCAGCGACCCACGCTCCTGCCCCACCTGCGGCACCGGCACGCTGT CGCTGAAGCTTGGCCGCTATGGCGCTTTTGTCGGCTGCTCCAACTATCCCGAATGCAAATATACCCGCCAGCTTGGTGGC GAGGCCAATGGCGAGACGGCGGCTTCCGACGAGCCGAAGCCGCTCGGCAAGGATCCGTTCACCGGCGAGGAAATCACCGC ACGCACCGGGCGCTTCGGCCCCTATATCCAGCGCGGCGAAGGCAAGGAAGCCAAGCGCGCCAGCCTCCCCAAGGGCTGGA CGCTCGACACGGTGGATCATGAAAAGGCGATAGCACTCCTGTCGCTGCCGCGCGATATCGGCCTGCACCCGGAAACCGGC AAGATGATTTCGGCGGGCATCGGGCGCTACGGGCCCTATGTCAGCCATGACGGCACATTCGCCAATCTGGAAAATGTCGA GGAAGTCTTCTCGGTCGGCCTCAACCGTGCAGTTTCCGTGCTTGCCGACAAGCAATCGAAGGGCGGCGGTCGGGGACGGT CCACACCGGCCGCGCTCGCCACCCTCGGCGATCATCCCGATGGCGGCGCTATCACCGTTCGCGACGGGCGCTACGGGCCT TATGTAAACTGGGGCAAGGTCAATGCGACACTGCCCAAGGGCAAGGATCCGGCATCGGTGACGCTGGAAGAGGCGCTGGC GCTGATTACTGAAAAAGCCGGTTCGTCAAAGGGCAGAAAGGCCCCCGCCCGCAAGTCGGCGGCAAGTTCTGAAAAGAAGA CAACCGCGAAAAAGACCGCTACAAAAAAAGCCCCGGCCAAAGCCAAGAGCAAGGCCAAGGCGGCAGAGGAGTAA
Upstream 100 bases:
>100_bases TCTTGACCAATCGCTGCCCGCTGTTCATGTGGAAGGCAACTTATTTCATGCGGCAAGGAGGTTTAGAGCCTCCGTGGGAC GCGAATTTAGGCTGCTTTAC
Downstream 100 bases:
>100_bases CAATTGGCACGCCGTTTTTCCAAATCCGATACCGTACGATCCGCACGGACCGAGCGGCGTGCAGCCACAGCCCAATCGAA AGATTCCGCAGCCTTTTTGC
Product: DNA topoisomerase I
Products: NA
Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]
Number of amino acids: Translated: 877; Mature: 877
Protein sequence:
>877_residues MNVVVVESPAKAKTINKYLGSDYKVLASFGHVRDLPAKDGSVRPDEDFAMSWEVDSNSAKRLADIVKALKDSDGLILATD PDREGEAISWHVLEVLRQKKALNGKTVKRVAFNAITKKAVLDAIAHPRDIDEPLVDAYLARRALDYLVGFTLSPVLWRKL PGARSAGRVQSVALRLVCDREAEIERFVREEYWNIAALLKTPRNDSFTARLTALDGKKLGKLDIKNGEQAGAIRTMLESA NFKAVSVEAKPTKRNPGPPFTTSTLQQAASGQLGFSASRTMQVAQRLYEGVDIGGETAGLITYMRTDGVQIAPEAINAAR DAIAKSFGAKYVPEKPRYYSSKAKNAQEAHEAIRPTDFSRHPKDVRRFLDEDQARLYELIWKRAIASQMQAADIERTTVD IEATNGSRAAMLRANGSVTKFDGFLAAYMDHREEEDDDEESAKLPEIRSGEALAREKIDATQHSTEPPPRYSEATLIKKM EELGIGRPSTYAATLATLRDREYIIIDKRKLIPEAKGRLVTAFLESFFERYVEYDFTADLEEKLDLISDGKLSWKEILRD FWRAFSGSVEDIKELRVTNVLDALNEELAPLVFPAREDGSDPRSCPTCGTGTLSLKLGRYGAFVGCSNYPECKYTRQLGG EANGETAASDEPKPLGKDPFTGEEITARTGRFGPYIQRGEGKEAKRASLPKGWTLDTVDHEKAIALLSLPRDIGLHPETG KMISAGIGRYGPYVSHDGTFANLENVEEVFSVGLNRAVSVLADKQSKGGGRGRSTPAALATLGDHPDGGAITVRDGRYGP YVNWGKVNATLPKGKDPASVTLEEALALITEKAGSSKGRKAPARKSAASSEKKTTAKKTATKKAPAKAKSKAKAAEE
Sequences:
>Translated_877_residues MNVVVVESPAKAKTINKYLGSDYKVLASFGHVRDLPAKDGSVRPDEDFAMSWEVDSNSAKRLADIVKALKDSDGLILATD PDREGEAISWHVLEVLRQKKALNGKTVKRVAFNAITKKAVLDAIAHPRDIDEPLVDAYLARRALDYLVGFTLSPVLWRKL PGARSAGRVQSVALRLVCDREAEIERFVREEYWNIAALLKTPRNDSFTARLTALDGKKLGKLDIKNGEQAGAIRTMLESA NFKAVSVEAKPTKRNPGPPFTTSTLQQAASGQLGFSASRTMQVAQRLYEGVDIGGETAGLITYMRTDGVQIAPEAINAAR DAIAKSFGAKYVPEKPRYYSSKAKNAQEAHEAIRPTDFSRHPKDVRRFLDEDQARLYELIWKRAIASQMQAADIERTTVD IEATNGSRAAMLRANGSVTKFDGFLAAYMDHREEEDDDEESAKLPEIRSGEALAREKIDATQHSTEPPPRYSEATLIKKM EELGIGRPSTYAATLATLRDREYIIIDKRKLIPEAKGRLVTAFLESFFERYVEYDFTADLEEKLDLISDGKLSWKEILRD FWRAFSGSVEDIKELRVTNVLDALNEELAPLVFPAREDGSDPRSCPTCGTGTLSLKLGRYGAFVGCSNYPECKYTRQLGG EANGETAASDEPKPLGKDPFTGEEITARTGRFGPYIQRGEGKEAKRASLPKGWTLDTVDHEKAIALLSLPRDIGLHPETG KMISAGIGRYGPYVSHDGTFANLENVEEVFSVGLNRAVSVLADKQSKGGGRGRSTPAALATLGDHPDGGAITVRDGRYGP YVNWGKVNATLPKGKDPASVTLEEALALITEKAGSSKGRKAPARKSAASSEKKTTAKKTATKKAPAKAKSKAKAAEE >Mature_877_residues MNVVVVESPAKAKTINKYLGSDYKVLASFGHVRDLPAKDGSVRPDEDFAMSWEVDSNSAKRLADIVKALKDSDGLILATD PDREGEAISWHVLEVLRQKKALNGKTVKRVAFNAITKKAVLDAIAHPRDIDEPLVDAYLARRALDYLVGFTLSPVLWRKL PGARSAGRVQSVALRLVCDREAEIERFVREEYWNIAALLKTPRNDSFTARLTALDGKKLGKLDIKNGEQAGAIRTMLESA NFKAVSVEAKPTKRNPGPPFTTSTLQQAASGQLGFSASRTMQVAQRLYEGVDIGGETAGLITYMRTDGVQIAPEAINAAR DAIAKSFGAKYVPEKPRYYSSKAKNAQEAHEAIRPTDFSRHPKDVRRFLDEDQARLYELIWKRAIASQMQAADIERTTVD IEATNGSRAAMLRANGSVTKFDGFLAAYMDHREEEDDDEESAKLPEIRSGEALAREKIDATQHSTEPPPRYSEATLIKKM EELGIGRPSTYAATLATLRDREYIIIDKRKLIPEAKGRLVTAFLESFFERYVEYDFTADLEEKLDLISDGKLSWKEILRD FWRAFSGSVEDIKELRVTNVLDALNEELAPLVFPAREDGSDPRSCPTCGTGTLSLKLGRYGAFVGCSNYPECKYTRQLGG EANGETAASDEPKPLGKDPFTGEEITARTGRFGPYIQRGEGKEAKRASLPKGWTLDTVDHEKAIALLSLPRDIGLHPETG KMISAGIGRYGPYVSHDGTFANLENVEEVFSVGLNRAVSVLADKQSKGGGRGRSTPAALATLGDHPDGGAITVRDGRYGP YVNWGKVNATLPKGKDPASVTLEEALALITEKAGSSKGRKAPARKSAASSEKKTTAKKTATKKAPAKAKSKAKAAEE
Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]
COG id: COG0550
COG function: function code L; Topoisomerase IA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 Toprim domain [H]
Homologues:
Organism=Homo sapiens, GI10835218, Length=618, Percent_Identity=25.2427184466019, Blast_Score=130, Evalue=4e-30, Organism=Homo sapiens, GI4507635, Length=594, Percent_Identity=23.7373737373737, Blast_Score=108, Evalue=2e-23, Organism=Escherichia coli, GI1787529, Length=677, Percent_Identity=38.4047267355982, Blast_Score=440, Evalue=1e-124, Organism=Escherichia coli, GI1788061, Length=561, Percent_Identity=25.668449197861, Blast_Score=126, Evalue=7e-30, Organism=Caenorhabditis elegans, GI17555378, Length=513, Percent_Identity=28.0701754385965, Blast_Score=113, Evalue=3e-25, Organism=Caenorhabditis elegans, GI32563869, Length=574, Percent_Identity=25.609756097561, Blast_Score=112, Evalue=1e-24, Organism=Saccharomyces cerevisiae, GI6323263, Length=567, Percent_Identity=24.1622574955908, Blast_Score=97, Evalue=8e-21, Organism=Drosophila melanogaster, GI24585251, Length=633, Percent_Identity=26.8562401263823, Blast_Score=149, Evalue=7e-36, Organism=Drosophila melanogaster, GI24640096, Length=568, Percent_Identity=22.7112676056338, Blast_Score=101, Evalue=3e-21,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003601 - InterPro: IPR013497 - InterPro: IPR013824 - InterPro: IPR013825 - InterPro: IPR000380 - InterPro: IPR003602 - InterPro: IPR013498 - InterPro: IPR005733 - InterPro: IPR006171 [H]
Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]
EC number: =5.99.1.2 [H]
Molecular weight: Translated: 95955; Mature: 95955
Theoretical pI: Translated: 9.06; Mature: 9.06
Prosite motif: PS00396 TOPOISOMERASE_I_PROK
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.6 %Cys (Translated Protein) 1.1 %Met (Translated Protein) 1.7 %Cys+Met (Translated Protein) 0.6 %Cys (Mature Protein) 1.1 %Met (Mature Protein) 1.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNVVVVESPAKAKTINKYLGSDYKVLASFGHVRDLPAKDGSVRPDEDFAMSWEVDSNSAK CCEEEECCCHHHHHHHHHHCCCHHHHHHHCCHHCCCCCCCCCCCCCCCEEEEEECCCHHH RLADIVKALKDSDGLILATDPDREGEAISWHVLEVLRQKKALNGKTVKRVAFNAITKKAV HHHHHHHHHCCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH LDAIAHPRDIDEPLVDAYLARRALDYLVGFTLSPVLWRKLPGARSAGRVQSVALRLVCDR HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC EAEIERFVREEYWNIAALLKTPRNDSFTARLTALDGKKLGKLDIKNGEQAGAIRTMLESA HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCEECCCCCHHHHHHHHHHCC NFKAVSVEAKPTKRNPGPPFTTSTLQQAASGQLGFSASRTMQVAQRLYEGVDIGGETAGL CCEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCE ITYMRTDGVQIAPEAINAARDAIAKSFGAKYVPEKPRYYSSKAKNAQEAHEAIRPTDFSR EEEEECCCCEECHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHCCCCCCCC HPKDVRRFLDEDQARLYELIWKRAIASQMQAADIERTTVDIEATNGSRAAMLRANGSVTK CHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEECCCCEEH FDGFLAAYMDHREEEDDDEESAKLPEIRSGEALAREKIDATQHSTEPPPRYSEATLIKKM HHHHHHHHHHHCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH EELGIGRPSTYAATLATLRDREYIIIDKRKLIPEAKGRLVTAFLESFFERYVEYDFTADL HHHCCCCCCHHHHHHHHHCCCCEEEEECHHHCHHHCCHHHHHHHHHHHHHHHCCCCCCCH EEKLDLISDGKLSWKEILRDFWRAFSGSVEDIKELRVTNVLDALNEELAPLVFPAREDGS HHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCC DPRSCPTCGTGTLSLKLGRYGAFVGCSNYPECKYTRQLGGEANGETAASDEPKPLGKDPF CCCCCCCCCCCEEEEEECCCEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCC TGEEITARTGRFGPYIQRGEGKEAKRASLPKGWTLDTVDHEKAIALLSLPRDIGLHPETG CCCHHHHCCCCCCCCEECCCCCCHHHHCCCCCCEECCCCHHHHHHHEECCHHCCCCCCCC KMISAGIGRYGPYVSHDGTFANLENVEEVFSVGLNRAVSVLADKQSKGGGRGRSTPAALA CHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH TLGDHPDGGAITVRDGRYGPYVNWGKVNATLPKGKDPASVTLEEALALITEKAGSSKGRK HCCCCCCCCEEEEECCCCCCCEECCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCC APARKSAASSEKKTTAKKTATKKAPAKAKSKAKAAEE CCCHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHCCCC >Mature Secondary Structure MNVVVVESPAKAKTINKYLGSDYKVLASFGHVRDLPAKDGSVRPDEDFAMSWEVDSNSAK CCEEEECCCHHHHHHHHHHCCCHHHHHHHCCHHCCCCCCCCCCCCCCCEEEEEECCCHHH RLADIVKALKDSDGLILATDPDREGEAISWHVLEVLRQKKALNGKTVKRVAFNAITKKAV HHHHHHHHHCCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH LDAIAHPRDIDEPLVDAYLARRALDYLVGFTLSPVLWRKLPGARSAGRVQSVALRLVCDR HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC EAEIERFVREEYWNIAALLKTPRNDSFTARLTALDGKKLGKLDIKNGEQAGAIRTMLESA HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCEECCCCCHHHHHHHHHHCC NFKAVSVEAKPTKRNPGPPFTTSTLQQAASGQLGFSASRTMQVAQRLYEGVDIGGETAGL CCEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCE ITYMRTDGVQIAPEAINAARDAIAKSFGAKYVPEKPRYYSSKAKNAQEAHEAIRPTDFSR EEEEECCCCEECHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHCCCCCCCC HPKDVRRFLDEDQARLYELIWKRAIASQMQAADIERTTVDIEATNGSRAAMLRANGSVTK CHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEECCCCEEH FDGFLAAYMDHREEEDDDEESAKLPEIRSGEALAREKIDATQHSTEPPPRYSEATLIKKM HHHHHHHHHHHCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH EELGIGRPSTYAATLATLRDREYIIIDKRKLIPEAKGRLVTAFLESFFERYVEYDFTADL HHHCCCCCCHHHHHHHHHCCCCEEEEECHHHCHHHCCHHHHHHHHHHHHHHHCCCCCCCH EEKLDLISDGKLSWKEILRDFWRAFSGSVEDIKELRVTNVLDALNEELAPLVFPAREDGS HHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCC DPRSCPTCGTGTLSLKLGRYGAFVGCSNYPECKYTRQLGGEANGETAASDEPKPLGKDPF CCCCCCCCCCCEEEEEECCCEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCC TGEEITARTGRFGPYIQRGEGKEAKRASLPKGWTLDTVDHEKAIALLSLPRDIGLHPETG CCCHHHHCCCCCCCCEECCCCCCHHHHCCCCCCEECCCCHHHHHHHEECCHHCCCCCCCC KMISAGIGRYGPYVSHDGTFANLENVEEVFSVGLNRAVSVLADKQSKGGGRGRSTPAALA CHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH TLGDHPDGGAITVRDGRYGPYVNWGKVNATLPKGKDPASVTLEEALALITEKAGSSKGRK HCCCCCCCCEEEEECCCCCCCEECCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCC APARKSAASSEKKTTAKKTATKKAPAKAKSKAKAAEE CCCHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA