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The map label for this gene is rimO [H]

Identifier: 225686353

GI number: 225686353

Start: 523349

End: 524662

Strand: Direct

Name: rimO [H]

Synonym: BMEA_B0539

Alternate gene names: 225686353

Gene position: 523349-524662 (Clockwise)

Preceding gene: 225686352

Following gene: 225686358

Centisome position: 44.15

GC content: 57.61

Gene sequence:

>1314_bases
ATGAGCGCACCGCGCGTCAGTTTTGTTTCACTTGGCTGCCCGAAGGCGCTTGTCGATTCCGAACGCATCATCACCGGCCT
CCGCTCTGAAGGCTATGAAATCTCCCGCAAACATGACGGTGCTGATCTAGTGATCGTTAATACCTGCGGCTTTCTCGATT
CTGCGCGTGACGAGTCGCTGGAAGCGATCGGCCTTGCACTGAACGAGAATGGCAAGGTGATCGTCACCGGCTGTCTTGGC
GCAGAGCCGGACGTTATCCGCGAGCGGCATCCGAATGTGCTCGCCATCACCGGCCCGCAAGCCTATGAAAGCGTGATGAA
TGCGGTGCACGAGGTTGCCCCGCCCGCCCATGACCCATTTGTCGATCTTGTGCCGCCGCAGGGCGTGAAGCTCACCCCGC
GCCATTATGCCTATCTGAAGATTTCCGAAGGCTGCTCCAACCGTTGTTCCTTCTGCATCATTCCGGCACTGCGCGGCGAT
CTGGTCTCGCGTCCGATCAACGAAGTGCTGCGCGAAGCTGAAAAACTGGTTCAGGCGGGTGTGAAGGAAATTCTCGTCAT
CTCGCAGGATACGAGCGCCTATGGCCTCGATATCAAGTATCAGGAAGCCATGTGGCAGGACCGCACGGTGCGTACGAAGT
TTCTCGACCTCTCGCGGGAACTGGGCGAGATGGGCGTTTGGGTGCGGATGCATTACGTCTATCCCTACCCTCACGTCGAT
GAAGTCATTCCGCTGATGGCCGAGGGCAAGATCCTGCCTTATCTGGATATTCCCTTCCAGCATGCGTCGCCCGCGGTTCT
GAAGAACATGCGTCGGCCTGCGCATCAGGAAAAAACCTCGCGCCGCATTCAGGCCTGGCGCGAAACCTGCCCCGATCTTG
CCGTGCGTTCCACCTTCATCGTCGGCTATCCGGGTGAAACGGAAGAAGATTTCCAGATGCTCCTCGATTGGCTGGATGAA
GCCAAGATCGAGCGTGCCGGTTGCTTTAAATATGAGGCGGTCAAGGGCGCGAAGGCTAATGATCTTGGCCTTGAACAGGT
GCCGGAAGAGGTGAAGGAAGCCCGCTGGCATCGCTTCATGGCAAAGCAGCAACAGATTTCAACCAACCTCTTGAAAAAGA
AGGTTGGCAAGCGTCTGCCGGTCATCATCGATGAAGCCAACGGCACCATTGGCAAGGGCCGCACCCGCTACGACGCGCCG
GAAATTGATGGCAGCGTTCATATCAGCTCGCGCCGCCCGCTGCGCGTGGGCGATATTGTGACGGTGAAGATCGAAGCAAG
TGATGCCTACGATCTGCACGGCACTGCTGTCTGA

Upstream 100 bases:

>100_bases
TCAAACCGCTGCGACACCGCAAAAGCGCCATCCAATCTTTACCTCCGCCCCCTTGTTGTGATAAGGCGCGCGCAATTTTC
CCAATTCGTAAGGTATTACA

Downstream 100 bases:

>100_bases
TAAGAGGTGAACCGCTTTTCGGACATGATGCGCGCTTCATTCATGAAGATCGAAACACGCTGCATAAGCAGTCCCATCAC
ATTGCAGGATAATAAAAAGG

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MSAPRVSFVSLGCPKALVDSERIITGLRSEGYEISRKHDGADLVIVNTCGFLDSARDESLEAIGLALNENGKVIVTGCLG
AEPDVIRERHPNVLAITGPQAYESVMNAVHEVAPPAHDPFVDLVPPQGVKLTPRHYAYLKISEGCSNRCSFCIIPALRGD
LVSRPINEVLREAEKLVQAGVKEILVISQDTSAYGLDIKYQEAMWQDRTVRTKFLDLSRELGEMGVWVRMHYVYPYPHVD
EVIPLMAEGKILPYLDIPFQHASPAVLKNMRRPAHQEKTSRRIQAWRETCPDLAVRSTFIVGYPGETEEDFQMLLDWLDE
AKIERAGCFKYEAVKGAKANDLGLEQVPEEVKEARWHRFMAKQQQISTNLLKKKVGKRLPVIIDEANGTIGKGRTRYDAP
EIDGSVHISSRRPLRVGDIVTVKIEASDAYDLHGTAV

Sequences:

>Translated_437_residues
MSAPRVSFVSLGCPKALVDSERIITGLRSEGYEISRKHDGADLVIVNTCGFLDSARDESLEAIGLALNENGKVIVTGCLG
AEPDVIRERHPNVLAITGPQAYESVMNAVHEVAPPAHDPFVDLVPPQGVKLTPRHYAYLKISEGCSNRCSFCIIPALRGD
LVSRPINEVLREAEKLVQAGVKEILVISQDTSAYGLDIKYQEAMWQDRTVRTKFLDLSRELGEMGVWVRMHYVYPYPHVD
EVIPLMAEGKILPYLDIPFQHASPAVLKNMRRPAHQEKTSRRIQAWRETCPDLAVRSTFIVGYPGETEEDFQMLLDWLDE
AKIERAGCFKYEAVKGAKANDLGLEQVPEEVKEARWHRFMAKQQQISTNLLKKKVGKRLPVIIDEANGTIGKGRTRYDAP
EIDGSVHISSRRPLRVGDIVTVKIEASDAYDLHGTAV
>Mature_436_residues
SAPRVSFVSLGCPKALVDSERIITGLRSEGYEISRKHDGADLVIVNTCGFLDSARDESLEAIGLALNENGKVIVTGCLGA
EPDVIRERHPNVLAITGPQAYESVMNAVHEVAPPAHDPFVDLVPPQGVKLTPRHYAYLKISEGCSNRCSFCIIPALRGDL
VSRPINEVLREAEKLVQAGVKEILVISQDTSAYGLDIKYQEAMWQDRTVRTKFLDLSRELGEMGVWVRMHYVYPYPHVDE
VIPLMAEGKILPYLDIPFQHASPAVLKNMRRPAHQEKTSRRIQAWRETCPDLAVRSTFIVGYPGETEEDFQMLLDWLDEA
KIERAGCFKYEAVKGAKANDLGLEQVPEEVKEARWHRFMAKQQQISTNLLKKKVGKRLPVIIDEANGTIGKGRTRYDAPE
IDGSVHISSRRPLRVGDIVTVKIEASDAYDLHGTAV

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI93277076, Length=391, Percent_Identity=27.8772378516624, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI28872784, Length=474, Percent_Identity=25.5274261603376, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI28872782, Length=474, Percent_Identity=25.5274261603376, Blast_Score=132, Evalue=5e-31,
Organism=Escherichia coli, GI1787057, Length=435, Percent_Identity=64.5977011494253, Blast_Score=586, Evalue=1e-169,
Organism=Escherichia coli, GI1786882, Length=445, Percent_Identity=26.2921348314607, Blast_Score=139, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17553146, Length=438, Percent_Identity=24.4292237442922, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71996771, Length=211, Percent_Identity=28.9099526066351, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21356207, Length=476, Percent_Identity=24.5798319327731, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI19922432, Length=325, Percent_Identity=27.6923076923077, Blast_Score=109, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 48775; Mature: 48643

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPRVSFVSLGCPKALVDSERIITGLRSEGYEISRKHDGADLVIVNTCGFLDSARDESL
CCCCCEEEEECCCCHHHHCCHHHHHHHHCCCEEECCCCCCCCEEEEEECCCCCCCCCCCC
EAIGLALNENGKVIVTGCLGAEPDVIRERHPNVLAITGPQAYESVMNAVHEVAPPAHDPF
EEEEEEECCCCCEEEEECCCCCCHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCE
VDLVPPQGVKLTPRHYAYLKISEGCSNRCSFCIIPALRGDLVSRPINEVLREAEKLVQAG
EECCCCCCCEECCCEEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHC
VKEILVISQDTSAYGLDIKYQEAMWQDRTVRTKFLDLSRELGEMGVWVRMHYVYPYPHVD
HHEEEEEECCCCCEEEEEEHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEEEEECCCCCHH
EVIPLMAEGKILPYLDIPFQHASPAVLKNMRRPAHQEKTSRRIQAWRETCPDLAVRSTFI
HHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHCEEE
VGYPGETEEDFQMLLDWLDEAKIERAGCFKYEAVKGAKANDLGLEQVPEEVKEARWHRFM
EECCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
AKQQQISTNLLKKKVGKRLPVIIDEANGTIGKGRTRYDAPEIDGSVHISSRRPLRVGDIV
HHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCEEECCEE
TVKIEASDAYDLHGTAV
EEEEECCCCCCCCCCCC
>Mature Secondary Structure 
SAPRVSFVSLGCPKALVDSERIITGLRSEGYEISRKHDGADLVIVNTCGFLDSARDESL
CCCCEEEEECCCCHHHHCCHHHHHHHHCCCEEECCCCCCCCEEEEEECCCCCCCCCCCC
EAIGLALNENGKVIVTGCLGAEPDVIRERHPNVLAITGPQAYESVMNAVHEVAPPAHDPF
EEEEEEECCCCCEEEEECCCCCCHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCE
VDLVPPQGVKLTPRHYAYLKISEGCSNRCSFCIIPALRGDLVSRPINEVLREAEKLVQAG
EECCCCCCCEECCCEEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHC
VKEILVISQDTSAYGLDIKYQEAMWQDRTVRTKFLDLSRELGEMGVWVRMHYVYPYPHVD
HHEEEEEECCCCCEEEEEEHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEEEEECCCCCHH
EVIPLMAEGKILPYLDIPFQHASPAVLKNMRRPAHQEKTSRRIQAWRETCPDLAVRSTFI
HHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHCEEE
VGYPGETEEDFQMLLDWLDEAKIERAGCFKYEAVKGAKANDLGLEQVPEEVKEARWHRFM
EECCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
AKQQQISTNLLKKKVGKRLPVIIDEANGTIGKGRTRYDAPEIDGSVHISSRRPLRVGDIV
HHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCEEECCEE
TVKIEASDAYDLHGTAV
EEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA