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The map label for this gene is oppA [H]

Identifier: 225686330

GI number: 225686330

Start: 500252

End: 501829

Strand: Reverse

Name: oppA [H]

Synonym: BMEA_B0512

Alternate gene names: 225686330

Gene position: 501829-500252 (Counterclockwise)

Preceding gene: 225686331

Following gene: 225686329

Centisome position: 42.33

GC content: 54.63

Gene sequence:

>1578_bases
ATGTATCGCAAATTTCTACTGATGGGCGTGTGTCTTCTGGCGCTCGCGGGCGCAGCCTCGGCAGAGGCGGTGCTGAACCG
CGGCAATGACACCGATCCGGCCACGCTCGATCATCATCACACGTCCACCGTTGCCGAAGGCAATGTGTTGCGCGATCTTT
ATGATGGCCTGACCATTCAGGATGCTGACGGCAAGGCCATTCCCGGCGTAGCGAAATCCTGGGATATTTCCGAGGATGGC
ACGGTCTATACCTTCCATCTGCGCGACAACGCGAAATGGTCGAACGGCGATCCGGTTACCGCTGGCGACTTCCAGTTCAC
CTTCCGCCGTCTGATGGACCCGAAAACGGCTGCCGGTTATGCCAGCATGTTGTTCATCATCAAGAATGCCGAAGATGCTG
CCGCTGGCAAGAAGCCTGTCGATCAGCTTGGCGTCGAGGCGGTTGACGATCATACGCTCAAGATCACGTTGAACTCCCCT
GCCCCTTATTTCCTTGAGCTTCTGACCCACCAGACAGGCTTCCCGCTGCATCAAAAAAGCGTTGAGGCCAATGGCGACAA
GTTCACCACGCCGGGCAAGATGGTTACGAACGGCGCCTATATGCTGGAAAGCTTCACGCCGAACGACAAGATCGTGATGA
AGAAGAATCCGTATTATTATGAAGCGGATCAGGTGAAGATCGATAAGGTCAACTGGATACCTTTCGAGGACCGCGCAAGC
TGCATGCGCCGCTTCGAGGCGAAAGAAGTACAGATCTGCTCCGATGTTCCGGCCGAGCAGATGGACTATGTGAAGAAGAA
CCTTTCCAAGGAATTCCGCATGGCGCCCTATCTCGGCGTCTATTACCTGCCGGTCAAGGGCAAGACTGCGGACAGCAAGC
TGAAAGATCCGCGCGTCCGCCAGGCGATTTCCATGGCTATCGACCGCGATTTCCTCGCCGATCAGGTCTGGCAGGGTACG
ATGATCCCAGGTTATTCCATGGTTCCGCCCGGCATCGCCAATTATGTGAAGGACGCGCCGAAGCTCGACTATTCCGACGA
CATGCTGGAGCGTGAGGACAAGGCCAAGGAATTGTTGAAGGAAGCAGGCGTGGAGCCGAATACGCTTTCCATCGAGCTTC
TCTACAATACGTCTGAAAACAACAAGAACACGATGGCAGCCATTGCAGACCAGCTTGGCAATATCGGCATCAAGGCCAGA
CTGAACGAAACCGAAGGCGCCAGCTATTTCAATTTCCTGCGCGAAGACGGCCCCTTCGATATCGCCCGCGCGAGCTGGAT
CGGCGATTATAATGATCCGCAGAACTTCCTGTACATCAGCCAGGGCAATGTCAGCTTCAACTATGCCAAGTGGAAGAACG
CCGATTATGATGCGCTGATGGCCAAGGCGGAAAAGACACAGGACCTGGGTGAACGAGCCAATATTCTGGCCGACGCCGCA
AAGATCCAGCTCAACGAATTGGGCGCTATTCCAATCCTTTATTATTCGTCGCTCGCGCTCGTTTCCAGCGATGTGGATGG
CTGGAACGACAACCTTATGAACAGCCGCAAGACGCGTTGGCTTTCCCTTAAAAAATAA

Upstream 100 bases:

>100_bases
TATTTGAATGAATTCAATACGTTGATAAGTTTGCTTGCAAGTACCACGCTTATCCATAATATGCGGGCCATTTCAAAAAT
TTCAACGGAGGGGTTCTTCG

Downstream 100 bases:

>100_bases
AATTTTCAGCTTGGGCTGCGCTTATCAGCGCGGCCCTTGTCCTTCCGGGGGGGCCCGAAGGGGCCGCGTTTTGACAAGCA
TTTTCCAAGAAAAGCATATG

Product: oligopeptide-binding protein oppA

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MYRKFLLMGVCLLALAGAASAEAVLNRGNDTDPATLDHHHTSTVAEGNVLRDLYDGLTIQDADGKAIPGVAKSWDISEDG
TVYTFHLRDNAKWSNGDPVTAGDFQFTFRRLMDPKTAAGYASMLFIIKNAEDAAAGKKPVDQLGVEAVDDHTLKITLNSP
APYFLELLTHQTGFPLHQKSVEANGDKFTTPGKMVTNGAYMLESFTPNDKIVMKKNPYYYEADQVKIDKVNWIPFEDRAS
CMRRFEAKEVQICSDVPAEQMDYVKKNLSKEFRMAPYLGVYYLPVKGKTADSKLKDPRVRQAISMAIDRDFLADQVWQGT
MIPGYSMVPPGIANYVKDAPKLDYSDDMLEREDKAKELLKEAGVEPNTLSIELLYNTSENNKNTMAAIADQLGNIGIKAR
LNETEGASYFNFLREDGPFDIARASWIGDYNDPQNFLYISQGNVSFNYAKWKNADYDALMAKAEKTQDLGERANILADAA
KIQLNELGAIPILYYSSLALVSSDVDGWNDNLMNSRKTRWLSLKK

Sequences:

>Translated_525_residues
MYRKFLLMGVCLLALAGAASAEAVLNRGNDTDPATLDHHHTSTVAEGNVLRDLYDGLTIQDADGKAIPGVAKSWDISEDG
TVYTFHLRDNAKWSNGDPVTAGDFQFTFRRLMDPKTAAGYASMLFIIKNAEDAAAGKKPVDQLGVEAVDDHTLKITLNSP
APYFLELLTHQTGFPLHQKSVEANGDKFTTPGKMVTNGAYMLESFTPNDKIVMKKNPYYYEADQVKIDKVNWIPFEDRAS
CMRRFEAKEVQICSDVPAEQMDYVKKNLSKEFRMAPYLGVYYLPVKGKTADSKLKDPRVRQAISMAIDRDFLADQVWQGT
MIPGYSMVPPGIANYVKDAPKLDYSDDMLEREDKAKELLKEAGVEPNTLSIELLYNTSENNKNTMAAIADQLGNIGIKAR
LNETEGASYFNFLREDGPFDIARASWIGDYNDPQNFLYISQGNVSFNYAKWKNADYDALMAKAEKTQDLGERANILADAA
KIQLNELGAIPILYYSSLALVSSDVDGWNDNLMNSRKTRWLSLKK
>Mature_525_residues
MYRKFLLMGVCLLALAGAASAEAVLNRGNDTDPATLDHHHTSTVAEGNVLRDLYDGLTIQDADGKAIPGVAKSWDISEDG
TVYTFHLRDNAKWSNGDPVTAGDFQFTFRRLMDPKTAAGYASMLFIIKNAEDAAAGKKPVDQLGVEAVDDHTLKITLNSP
APYFLELLTHQTGFPLHQKSVEANGDKFTTPGKMVTNGAYMLESFTPNDKIVMKKNPYYYEADQVKIDKVNWIPFEDRAS
CMRRFEAKEVQICSDVPAEQMDYVKKNLSKEFRMAPYLGVYYLPVKGKTADSKLKDPRVRQAISMAIDRDFLADQVWQGT
MIPGYSMVPPGIANYVKDAPKLDYSDDMLEREDKAKELLKEAGVEPNTLSIELLYNTSENNKNTMAAIADQLGNIGIKAR
LNETEGASYFNFLREDGPFDIARASWIGDYNDPQNFLYISQGNVSFNYAKWKNADYDALMAKAEKTQDLGERANILADAA
KIQLNELGAIPILYYSSLALVSSDVDGWNDNLMNSRKTRWLSLKK

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=517, Percent_Identity=37.330754352031, Blast_Score=327, Evalue=2e-90,
Organism=Escherichia coli, GI87081878, Length=495, Percent_Identity=35.5555555555556, Blast_Score=288, Evalue=8e-79,
Organism=Escherichia coli, GI1789397, Length=523, Percent_Identity=33.2695984703633, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1787052, Length=520, Percent_Identity=26.5384615384615, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1789966, Length=533, Percent_Identity=25.140712945591, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI1787762, Length=531, Percent_Identity=26.3653483992467, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1787551, Length=469, Percent_Identity=22.8144989339019, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1789887, Length=482, Percent_Identity=24.896265560166, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58622; Mature: 58622

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRKFLLMGVCLLALAGAASAEAVLNRGNDTDPATLDHHHTSTVAEGNVLRDLYDGLTIQ
CCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCHHHCCCHHHHHHCCCEEE
DADGKAIPGVAKSWDISEDGTVYTFHLRDNAKWSNGDPVTAGDFQFTFRRLMDPKTAAGY
CCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCHHCCC
ASMLFIIKNAEDAAAGKKPVDQLGVEAVDDHTLKITLNSPAPYFLELLTHQTGFPLHQKS
EEEEEEEECCCHHCCCCCCHHHHCCEEECCCEEEEEECCCCHHHHHHHHHCCCCCCCCCC
VEANGDKFTTPGKMVTNGAYMLESFTPNDKIVMKKNPYYYEADQVKIDKVNWIPFEDRAS
CCCCCCEECCCCCEEECCEEEEEECCCCCEEEEECCCCEEECCCEEEEEEEECCCHHHHH
CMRRFEAKEVQICSDVPAEQMDYVKKNLSKEFRMAPYLGVYYLPVKGKTADSKLKDPRVR
HHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHH
QAISMAIDRDFLADQVWQGTMIPGYSMVPPGIANYVKDAPKLDYSDDMLEREDKAKELLK
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
EAGVEPNTLSIELLYNTSENNKNTMAAIADQLGNIGIKARLNETEGASYFNFLREDGPFD
HCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHCCCCCC
IARASWIGDYNDPQNFLYISQGNVSFNYAKWKNADYDALMAKAEKTQDLGERANILADAA
EEHHHCCCCCCCCCCEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KIQLNELGAIPILYYSSLALVSSDVDGWNDNLMNSRKTRWLSLKK
HEEHHHCCCCHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEECCC
>Mature Secondary Structure
MYRKFLLMGVCLLALAGAASAEAVLNRGNDTDPATLDHHHTSTVAEGNVLRDLYDGLTIQ
CCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCHHHCCCHHHHHHCCCEEE
DADGKAIPGVAKSWDISEDGTVYTFHLRDNAKWSNGDPVTAGDFQFTFRRLMDPKTAAGY
CCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCHHCCC
ASMLFIIKNAEDAAAGKKPVDQLGVEAVDDHTLKITLNSPAPYFLELLTHQTGFPLHQKS
EEEEEEEECCCHHCCCCCCHHHHCCEEECCCEEEEEECCCCHHHHHHHHHCCCCCCCCCC
VEANGDKFTTPGKMVTNGAYMLESFTPNDKIVMKKNPYYYEADQVKIDKVNWIPFEDRAS
CCCCCCEECCCCCEEECCEEEEEECCCCCEEEEECCCCEEECCCEEEEEEEECCCHHHHH
CMRRFEAKEVQICSDVPAEQMDYVKKNLSKEFRMAPYLGVYYLPVKGKTADSKLKDPRVR
HHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHH
QAISMAIDRDFLADQVWQGTMIPGYSMVPPGIANYVKDAPKLDYSDDMLEREDKAKELLK
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
EAGVEPNTLSIELLYNTSENNKNTMAAIADQLGNIGIKARLNETEGASYFNFLREDGPFD
HCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHCCCCCC
IARASWIGDYNDPQNFLYISQGNVSFNYAKWKNADYDALMAKAEKTQDLGERANILADAA
EEHHHCCCCCCCCCCEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KIQLNELGAIPILYYSSLALVSSDVDGWNDNLMNSRKTRWLSLKK
HEEHHHCCCCHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2821267; 3525163; 11677609; 8202710; 8747465; 10422831; 10438628 [H]