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The map label for this gene is oppA [H]

Identifier: 225686329

GI number: 225686329

Start: 498564

End: 500141

Strand: Reverse

Name: oppA [H]

Synonym: BMEA_B0511

Alternate gene names: 225686329

Gene position: 500141-498564 (Counterclockwise)

Preceding gene: 225686330

Following gene: 225686328

Centisome position: 42.19

GC content: 53.23

Gene sequence:

>1578_bases
ATGGTTCGCGAAATTTTGATGACAGGCATTTGCCTTCTGGCGTTGAACAGTGCCGCATCGGCACAAACAGTGCTCAACCG
GGGTAACGATACCGATCCCGCGACACTGGATCAGCACCATACTTCGACCGTTTCCGAAAACCGCGTTCTCCGCGACCTTT
ATGAAGGTCTTCTGGCGCAAAATGCCAAGGGCGAAGCCATTCCGGGCGCTGCCTCCTCTTGGGATATTTCGGAAGACGGC
AAGGTCTATACCTTTCATATGCGCGACGGCGCAAAATGGTCAAACGGTGATCCGGTGACGGCTGGCGACTTCGAGTTCTC
GTTCCGCCGCATCATGGACCCGAAGACGGCAGCCGGTTATGCAAGCGTTCTTTATGCCATCCAGAATGGCGAAGAAGTTG
CAACCGGAAAAATGCCGGTGGACCAGCTTGGCGTGAAGGCGCTGGACGACAAGACACTCCAGATCACGTTGAAAAGCCCT
GCCCCTTATTTTCTTGAGCTTCTGACGCACCAGACCGGCTTTCCGGTCAACAAGAAAGCTGTCGAGAAGTTTGGTGACAA
GTTCACCCTTCCCGGCAATCTCGTGACCAATGGCCCTTACCAGCTTGTCAGCTTCGTACCGAATGACAAGATCGTCATGA
AAAAGAACCCGAACTACTGGGATGCCGAGAACGTCAAGATCGACACGGTCAACTGGATGCCCTTTGAAGACCGGTCGAGC
TGTATGCGCCGCTTTGAGGCAAAGGAAGTGGATATCTGCTCCGATGTTTCTGCCGAGCAGATGGACTATGTGAAGAAGAA
CCTTTCCAAGGAATTCCGCCTCGCGCCCTATCTTGGCATTTATTACGTGGACATTAAGGGCGAGCCTTCCAGCAAACTGC
GCGATCCGCGTGTGCGCCGCGCCATTTCTCTGGCCGCGGACCGGGAATTCATGGCGGATGAGGTCTGGCGTGGCGTCATG
CTGCCCGCCTATTCCATGGTGCCGCCCGGCATCGCCAATTACGTGAAGGATGCACCGAAGCTCGACTTTGCCAATGAAGA
CATTCTCGACCGCGAAGACAAGGCGAAGGAGCTTTTGAAGGAAGCAGGCGTGGAGCCCAATACGCTGTCGGTGACGCTGC
GCTACAACACATCGGAAAATCACAAGAATACGATGGCAGCCTTTGCCAACATGCTGAAAAACATCGGCATCAATGCCAAG
CTGGATGAGGTCGAAGGCACGACCTATTTCAACTATCTTCAGGAAAAAGGCATGTTCGACCTGACCCGTGACGGCTGGAT
CGGAGATTATAACGATCCGAATTCGTTCCTGGAACTTTATACAACCGGCAATTATTTCAATTATGCCGAATGGTCGAACA
AGGATTACGATGCGCTGATGGATAAATCTGCTGTCACGACCGATCTCGCCGAACGTGCGAAGATCCTGGCTGAGGCAGAA
GAGATTCTCCTGAAGGAAGGGGCTATCGTGCCATTGATGTATTATTCGTCGACCGCATTGGTTGCCGACAGGGTTCAAGG
TTACGACGATAACCTCATGAACTCGCACGGCACGCGCTGGCTATCCGTCAAGAACTAA

Upstream 100 bases:

>100_bases
TTGGGCTGCGCTTATCAGCGCGGCCCTTGTCCTTCCGGGGGGGCCCGAAGGGGCCGCGTTTTGACAAGCATTTTCCAAGA
AAAGCATATGGGAGTTTTCG

Downstream 100 bases:

>100_bases
CAAAACGAAACCCCTTAGGGGTTTGCCGGGCCGTTTGGAACGCTGCATGAGAGCATTTTCGAGCCAAAAGTGTGAAACGC
CTACGCGGGGAAATCAGTCC

Product: oligopeptide-binding protein oppA

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MVREILMTGICLLALNSAASAQTVLNRGNDTDPATLDQHHTSTVSENRVLRDLYEGLLAQNAKGEAIPGAASSWDISEDG
KVYTFHMRDGAKWSNGDPVTAGDFEFSFRRIMDPKTAAGYASVLYAIQNGEEVATGKMPVDQLGVKALDDKTLQITLKSP
APYFLELLTHQTGFPVNKKAVEKFGDKFTLPGNLVTNGPYQLVSFVPNDKIVMKKNPNYWDAENVKIDTVNWMPFEDRSS
CMRRFEAKEVDICSDVSAEQMDYVKKNLSKEFRLAPYLGIYYVDIKGEPSSKLRDPRVRRAISLAADREFMADEVWRGVM
LPAYSMVPPGIANYVKDAPKLDFANEDILDREDKAKELLKEAGVEPNTLSVTLRYNTSENHKNTMAAFANMLKNIGINAK
LDEVEGTTYFNYLQEKGMFDLTRDGWIGDYNDPNSFLELYTTGNYFNYAEWSNKDYDALMDKSAVTTDLAERAKILAEAE
EILLKEGAIVPLMYYSSTALVADRVQGYDDNLMNSHGTRWLSVKN

Sequences:

>Translated_525_residues
MVREILMTGICLLALNSAASAQTVLNRGNDTDPATLDQHHTSTVSENRVLRDLYEGLLAQNAKGEAIPGAASSWDISEDG
KVYTFHMRDGAKWSNGDPVTAGDFEFSFRRIMDPKTAAGYASVLYAIQNGEEVATGKMPVDQLGVKALDDKTLQITLKSP
APYFLELLTHQTGFPVNKKAVEKFGDKFTLPGNLVTNGPYQLVSFVPNDKIVMKKNPNYWDAENVKIDTVNWMPFEDRSS
CMRRFEAKEVDICSDVSAEQMDYVKKNLSKEFRLAPYLGIYYVDIKGEPSSKLRDPRVRRAISLAADREFMADEVWRGVM
LPAYSMVPPGIANYVKDAPKLDFANEDILDREDKAKELLKEAGVEPNTLSVTLRYNTSENHKNTMAAFANMLKNIGINAK
LDEVEGTTYFNYLQEKGMFDLTRDGWIGDYNDPNSFLELYTTGNYFNYAEWSNKDYDALMDKSAVTTDLAERAKILAEAE
EILLKEGAIVPLMYYSSTALVADRVQGYDDNLMNSHGTRWLSVKN
>Mature_525_residues
MVREILMTGICLLALNSAASAQTVLNRGNDTDPATLDQHHTSTVSENRVLRDLYEGLLAQNAKGEAIPGAASSWDISEDG
KVYTFHMRDGAKWSNGDPVTAGDFEFSFRRIMDPKTAAGYASVLYAIQNGEEVATGKMPVDQLGVKALDDKTLQITLKSP
APYFLELLTHQTGFPVNKKAVEKFGDKFTLPGNLVTNGPYQLVSFVPNDKIVMKKNPNYWDAENVKIDTVNWMPFEDRSS
CMRRFEAKEVDICSDVSAEQMDYVKKNLSKEFRLAPYLGIYYVDIKGEPSSKLRDPRVRRAISLAADREFMADEVWRGVM
LPAYSMVPPGIANYVKDAPKLDFANEDILDREDKAKELLKEAGVEPNTLSVTLRYNTSENHKNTMAAFANMLKNIGINAK
LDEVEGTTYFNYLQEKGMFDLTRDGWIGDYNDPNSFLELYTTGNYFNYAEWSNKDYDALMDKSAVTTDLAERAKILAEAE
EILLKEGAIVPLMYYSSTALVADRVQGYDDNLMNSHGTRWLSVKN

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=513, Percent_Identity=38.7914230019493, Blast_Score=349, Evalue=3e-97,
Organism=Escherichia coli, GI87081878, Length=491, Percent_Identity=35.8452138492872, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1789397, Length=497, Percent_Identity=34.20523138833, Blast_Score=294, Evalue=8e-81,
Organism=Escherichia coli, GI1787052, Length=512, Percent_Identity=25.1953125, Blast_Score=148, Evalue=7e-37,
Organism=Escherichia coli, GI1789966, Length=548, Percent_Identity=25.7299270072993, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1787762, Length=511, Percent_Identity=25.6360078277886, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1787551, Length=448, Percent_Identity=23.8839285714286, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1789887, Length=473, Percent_Identity=24.9471458773784, Blast_Score=85, Evalue=9e-18,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58900; Mature: 58900

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVREILMTGICLLALNSAASAQTVLNRGNDTDPATLDQHHTSTVSENRVLRDLYEGLLAQ
CHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
NAKGEAIPGAASSWDISEDGKVYTFHMRDGAKWSNGDPVTAGDFEFSFRRIMDPKTAAGY
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHH
ASVLYAIQNGEEVATGKMPVDQLGVKALDDKTLQITLKSPAPYFLELLTHQTGFPVNKKA
HHHHHHHCCCCEEECCCCCHHHHCCEEECCCEEEEEECCCCHHHHHHHHHCCCCCCCHHH
VEKFGDKFTLPGNLVTNGPYQLVSFVPNDKIVMKKNPNYWDAENVKIDTVNWMPFEDRSS
HHHCCCEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCEEEEEEECCCCCCHHH
CMRRFEAKEVDICSDVSAEQMDYVKKNLSKEFRLAPYLGIYYVDIKGEPSSKLRDPRVRR
HHHHHCCCCCCHHCCCCHHHHHHHHHHCCCHHCCCCEEEEEEEEECCCCCHHCCCHHHHH
AISLAADREFMADEVWRGVMLPAYSMVPPGIANYVKDAPKLDFANEDILDREDKAKELLK
HHHHHHHHHHHHHHHHCCHHCCCHHCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
EAGVEPNTLSVTLRYNTSENHKNTMAAFANMLKNIGINAKLDEVEGTTYFNYLQEKGMFD
HCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHCCCEE
LTRDGWIGDYNDPNSFLELYTTGNYFNYAEWSNKDYDALMDKSAVTTDLAERAKILAEAE
ECCCCCCCCCCCCHHEEEEEECCCEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHH
EILLKEGAIVPLMYYSSTALVADRVQGYDDNLMNSHGTRWLSVKN
HHHHHCCCEEEEEEECCHHHHHHHHCCCCCHHHCCCCCEEEEECC
>Mature Secondary Structure
MVREILMTGICLLALNSAASAQTVLNRGNDTDPATLDQHHTSTVSENRVLRDLYEGLLAQ
CHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
NAKGEAIPGAASSWDISEDGKVYTFHMRDGAKWSNGDPVTAGDFEFSFRRIMDPKTAAGY
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHH
ASVLYAIQNGEEVATGKMPVDQLGVKALDDKTLQITLKSPAPYFLELLTHQTGFPVNKKA
HHHHHHHCCCCEEECCCCCHHHHCCEEECCCEEEEEECCCCHHHHHHHHHCCCCCCCHHH
VEKFGDKFTLPGNLVTNGPYQLVSFVPNDKIVMKKNPNYWDAENVKIDTVNWMPFEDRSS
HHHCCCEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCEEEEEEECCCCCCHHH
CMRRFEAKEVDICSDVSAEQMDYVKKNLSKEFRLAPYLGIYYVDIKGEPSSKLRDPRVRR
HHHHHCCCCCCHHCCCCHHHHHHHHHHCCCHHCCCCEEEEEEEEECCCCCHHCCCHHHHH
AISLAADREFMADEVWRGVMLPAYSMVPPGIANYVKDAPKLDFANEDILDREDKAKELLK
HHHHHHHHHHHHHHHHCCHHCCCHHCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
EAGVEPNTLSVTLRYNTSENHKNTMAAFANMLKNIGINAKLDEVEGTTYFNYLQEKGMFD
HCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHCCCEE
LTRDGWIGDYNDPNSFLELYTTGNYFNYAEWSNKDYDALMDKSAVTTDLAERAKILAEAE
ECCCCCCCCCCCCHHEEEEEECCCEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHH
EILLKEGAIVPLMYYSSTALVADRVQGYDDNLMNSHGTRWLSVKN
HHHHHCCCEEEEEEECCHHHHHHHHCCCCCHHHCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2821267; 3525163; 11677609; 8202710; 8747465; 10422831; 10438628 [H]