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The map label for this gene is bkdA2 [H]

Identifier: 225686317

GI number: 225686317

Start: 483318

End: 484331

Strand: Direct

Name: bkdA2 [H]

Synonym: BMEA_B0498

Alternate gene names: 225686317

Gene position: 483318-484331 (Clockwise)

Preceding gene: 225686316

Following gene: 225686318

Centisome position: 40.77

GC content: 59.57

Gene sequence:

>1014_bases
ATGACGAAAATGACCATGATCGAGGCGATCCAGAACGCCCACGACATTGCCATGGAACGCGATCAAAAAGTGGTCGTGTT
CGGCGAGGATGTCGGTTATTTCGGCGGCGTCTTCCGCTGCACCGCCGGATTGCAGAAGAAATACGGCAAGGAACGCTGCT
TCGATGCGCCGATCAGCGAGCTTGGCATCGTCGGCACGGCAATCGGCATGGCCGTTTACGGCCTGCGGCCCTGCATCGAA
GTGCAGTTCGCCGATTATGTCTATCCGGCCTATGACCAGATCGTTTCGGAAGCCGCGCGCCTTCGCTATCGTTCGGCAGG
CGAATTTACCTGCCCGATCGTGATCCGTATGCCTTCCGGCGGCGGTATTTACGGCGGACAGACCCATAGCCAGAGCCCTG
AGGCGCTATTTACCCATGTTTCCGGCCTCAAGACCGTTATGCCGTCAACGCCTGCCGACGCTAAGGGCCTGCTGCTCGCA
GCTATCGAAGATCCCGATCCGGTCATCATGTTCGAGCCGAAGCGCCTTTATAACGGCCCGTTCGATGGCCATCATGACAA
GCCGGTCACGTCATGGAAGAAGCATGATCTGGGCGAGGTGCCGGAAGGCTACTATACCGTGCCGCTCGGCAAGGCCGCGA
TCCGCCGCGAAGGCAGCGATGTGACGGTGCTGGCCTATGGCACCATGGTTCATGTGGCTTTGGCGGCTGCCGAAGAAACC
GGCGTCGATGCGGAAGTCATCGATCTGCGCACGTTGCTGCCGCTCGACACTGAAACGATCATGGCTTCGGTGAAGAAGAT
CGGGCGATGCATTATCGTGCATGAGGCGACGCTGACCTGTGGTTATGGCGCGGAGCTTGCCGCACTCGTTCAGCGCGATT
GCTTCTATCATCTGGAAGCGCCGATCATGCGCGTCACCGGTTGGGGCACGCCTTATCCCCATGCACAGGAATGGGCCTAT
TTCCCCGGCCCGGACCGCGTCGGTCGCGCGCTCGTATCGATCATGGAGGCTTAA

Upstream 100 bases:

>100_bases
TTTCCAGGAAAAGTGTGAAGCGCCTACGTAGGGAAACCAGTCCACTGGACTGGTTTCTGATCCTGCTTCGATCCGTTCGG
AAATGCGGAGGAAGTAATCT

Downstream 100 bases:

>100_bases
ATGGCTCATTTTGCAATCAAGCTCCCTGATGTTGGCGAAGGCGTTGCGGAAGCAGAACTTGTCGAATGGCATGTGAAAGT
GGGCGACATCGTGCGCGAGG

Product: 2-oxoisovalerate dehydrogenase subunit beta

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; BCKDH E1-beta [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MTKMTMIEAIQNAHDIAMERDQKVVVFGEDVGYFGGVFRCTAGLQKKYGKERCFDAPISELGIVGTAIGMAVYGLRPCIE
VQFADYVYPAYDQIVSEAARLRYRSAGEFTCPIVIRMPSGGGIYGGQTHSQSPEALFTHVSGLKTVMPSTPADAKGLLLA
AIEDPDPVIMFEPKRLYNGPFDGHHDKPVTSWKKHDLGEVPEGYYTVPLGKAAIRREGSDVTVLAYGTMVHVALAAAEET
GVDAEVIDLRTLLPLDTETIMASVKKIGRCIIVHEATLTCGYGAELAALVQRDCFYHLEAPIMRVTGWGTPYPHAQEWAY
FPGPDRVGRALVSIMEA

Sequences:

>Translated_337_residues
MTKMTMIEAIQNAHDIAMERDQKVVVFGEDVGYFGGVFRCTAGLQKKYGKERCFDAPISELGIVGTAIGMAVYGLRPCIE
VQFADYVYPAYDQIVSEAARLRYRSAGEFTCPIVIRMPSGGGIYGGQTHSQSPEALFTHVSGLKTVMPSTPADAKGLLLA
AIEDPDPVIMFEPKRLYNGPFDGHHDKPVTSWKKHDLGEVPEGYYTVPLGKAAIRREGSDVTVLAYGTMVHVALAAAEET
GVDAEVIDLRTLLPLDTETIMASVKKIGRCIIVHEATLTCGYGAELAALVQRDCFYHLEAPIMRVTGWGTPYPHAQEWAY
FPGPDRVGRALVSIMEA
>Mature_336_residues
TKMTMIEAIQNAHDIAMERDQKVVVFGEDVGYFGGVFRCTAGLQKKYGKERCFDAPISELGIVGTAIGMAVYGLRPCIEV
QFADYVYPAYDQIVSEAARLRYRSAGEFTCPIVIRMPSGGGIYGGQTHSQSPEALFTHVSGLKTVMPSTPADAKGLLLAA
IEDPDPVIMFEPKRLYNGPFDGHHDKPVTSWKKHDLGEVPEGYYTVPLGKAAIRREGSDVTVLAYGTMVHVALAAAEETG
VDAEVIDLRTLLPLDTETIMASVKKIGRCIIVHEATLTCGYGAELAALVQRDCFYHLEAPIMRVTGWGTPYPHAQEWAYF
PGPDRVGRALVSIMEA

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4557353, Length=339, Percent_Identity=48.377581120944, Blast_Score=309, Evalue=2e-84,
Organism=Homo sapiens, GI34101272, Length=339, Percent_Identity=48.377581120944, Blast_Score=309, Evalue=2e-84,
Organism=Homo sapiens, GI156564403, Length=318, Percent_Identity=38.0503144654088, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI291084858, Length=318, Percent_Identity=36.1635220125786, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17506935, Length=335, Percent_Identity=48.6567164179104, Blast_Score=303, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI17538422, Length=336, Percent_Identity=39.8809523809524, Blast_Score=221, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6319698, Length=327, Percent_Identity=37.9204892966361, Blast_Score=219, Evalue=4e-58,
Organism=Drosophila melanogaster, GI160714832, Length=339, Percent_Identity=49.2625368731563, Blast_Score=324, Evalue=6e-89,
Organism=Drosophila melanogaster, GI160714828, Length=339, Percent_Identity=49.2625368731563, Blast_Score=324, Evalue=6e-89,
Organism=Drosophila melanogaster, GI21358145, Length=318, Percent_Identity=38.6792452830189, Blast_Score=222, Evalue=3e-58,
Organism=Drosophila melanogaster, GI24650940, Length=318, Percent_Identity=38.6792452830189, Blast_Score=222, Evalue=3e-58,
Organism=Drosophila melanogaster, GI24650943, Length=90, Percent_Identity=38.8888888888889, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24650945, Length=90, Percent_Identity=38.8888888888889, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI160714826, Length=66, Percent_Identity=50, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 36779; Mature: 36648

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKMTMIEAIQNAHDIAMERDQKVVVFGEDVGYFGGVFRCTAGLQKKYGKERCFDAPISE
CCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCHHHHCCCCHHH
LGIVGTAIGMAVYGLRPCIEVQFADYVYPAYDQIVSEAARLRYRSAGEFTCPIVIRMPSG
HHHHHHHHHHHHHCCCCCEEEEEHHHCCHHHHHHHHHHHHHHHCCCCCEECEEEEEECCC
GGIYGGQTHSQSPEALFTHVSGLKTVMPSTPADAKGLLLAAIEDPDPVIMFEPKRLYNGP
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEECCHHHHCCC
FDGHHDKPVTSWKKHDLGEVPEGYYTVPLGKAAIRREGSDVTVLAYGTMVHVALAAAEET
CCCCCCCCCCCCHHCCCCCCCCCEEEECCCHHHHHCCCCCEEEEEEHHHHHHHHHHHHHC
GVDAEVIDLRTLLPLDTETIMASVKKIGRCIIVHEATLTCGYGAELAALVQRDCFYHLEA
CCCCEEEEHHHHCCCCHHHHHHHHHHHCCEEEEEECEEECCCCHHHHHHHHHHHHHCCCC
PIMRVTGWGTPYPHAQEWAYFPGPDRVGRALVSIMEA
CEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKMTMIEAIQNAHDIAMERDQKVVVFGEDVGYFGGVFRCTAGLQKKYGKERCFDAPISE
CCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCHHHHCCCCHHH
LGIVGTAIGMAVYGLRPCIEVQFADYVYPAYDQIVSEAARLRYRSAGEFTCPIVIRMPSG
HHHHHHHHHHHHHCCCCCEEEEEHHHCCHHHHHHHHHHHHHHHCCCCCEECEEEEEECCC
GGIYGGQTHSQSPEALFTHVSGLKTVMPSTPADAKGLLLAAIEDPDPVIMFEPKRLYNGP
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEECCHHHHCCC
FDGHHDKPVTSWKKHDLGEVPEGYYTVPLGKAAIRREGSDVTVLAYGTMVHVALAAAEET
CCCCCCCCCCCCHHCCCCCCCCCEEEECCCHHHHHCCCCCEEEEEEHHHHHHHHHHHHHC
GVDAEVIDLRTLLPLDTETIMASVKKIGRCIIVHEATLTCGYGAELAALVQRDCFYHLEA
CCCCEEEEHHHHCCCCHHHHHHHHHHHCCEEEEEECEEECCCCHHHHHHHHHHHHHCCCC
PIMRVTGWGTPYPHAQEWAYFPGPDRVGRALVSIMEA
CEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]