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The map label for this gene is bkdB [H]

Identifier: 225686318

GI number: 225686318

Start: 484332

End: 485627

Strand: Direct

Name: bkdB [H]

Synonym: BMEA_B0499

Alternate gene names: 225686318

Gene position: 484332-485627 (Clockwise)

Preceding gene: 225686317

Following gene: 225686319

Centisome position: 40.85

GC content: 60.26

Gene sequence:

>1296_bases
ATGGCTCATTTTGCAATCAAGCTCCCTGATGTTGGCGAAGGCGTTGCGGAAGCAGAACTTGTCGAATGGCATGTGAAAGT
GGGCGACATCGTGCGCGAGGACGATCTTCTCGCTGCTGTCATGACCGACAAGGCGACGGTGGAAATTCCCTCCTCACGCG
CAGGCAAGGTGATCGCCATCAACGGCGAAGTGGGCGAGAAGATCGCTGTTGGCTCCGAACTCGTGCGCCTTGAGATTGAA
GGCGGTGCGACGGAAAAAGCCGAAGAACCGCAACCGCAGGCGGCTGAGCCGACCGCGCCTGCTGCTGCAGTCCCGGAAGC
GCCGGTTCTTCTGCAAACGCCTGTGCCGGAAAAACCTGCCGCGCCGAAGCGTGAAAATGCTGGTCGCCCGTTCACCGGTG
CTGGCCCATTGCGCCCCGAAGGCGAAAAGCCGCTCGCCACGCCGTCGGTGAGGCTACGCGCGCGTGATGCGGGCATCGAT
CTGCGCCGCGTTCGCGGCACCGGACCGGCCGGGCGCATCACGCATGAAGACTTGGATGCGTTTTTCCAGATGGAAAGCGG
TGCCGCTCCGGCACTCTCCGGCTATGCAGCCGATACATCCATCAATGAAATCAAGGTCATCGGCCTCAGGCGCAAGATTG
CGGAGCGCATGGCGGAAGCCAAGCGCCACATTCCGCATATCACCATTGTCGAGGAAGTGGACGTCACCCAGCTTGAAGAG
CTGCGCAACGGCCTCAACCATGAAAAGAAGGAGGGGCACCCACGCCTCACACTTCTGCCGTTCATCATCCGCACAATCGT
AAAGGCGGTGAAGGAACAGCCGGGCCTCAACGCGCATTTTGACGATGAGGCGGATATTATCCGCCAGTTTGGCGGCGTGC
ATGTCGGCATCGCCACGCAGACGCCGAATGGCCTCATTGTGCCCGTCGTGCGCCATGCCGAAAGCATGAGCGTGTTTGCG
GCGGCGACCGAGCTTTCCCGTGTTACCGAGGCCGCGCGCAACGGAACAGCCAAGCGTGAGGAGCTGACCGGTTCCACCAT
CACCATCACATCCCTTGGGCCTCTTGGCGCCATTGCCACGACGCCGATCATCAACCGGCCTGAAGTGGCAATTGTCGGCA
TCAACAAGATGGCCGTTCGTCCGATGTGGGATGGCACGCAATTCGTGCCGCGCAAGATGATGAACCTTTCATGCAGTTTC
GATCACCGGGTCATCGACGGCTGGGACGCTGCCGTCTTCGTGCAGAAGCTGAAAAGCCTGCTCGAAACGCCTGCCATGAT
TTTTGTAGAGGGATGA

Upstream 100 bases:

>100_bases
CACCGGTTGGGGCACGCCTTATCCCCATGCACAGGAATGGGCCTATTTCCCCGGCCCGGACCGCGTCGGTCGCGCGCTCG
TATCGATCATGGAGGCTTAA

Downstream 100 bases:

>100_bases
CCAGACGAGCGAGATTTCCTGCAAACTTCTGATTATCGGCGGCGGTCCCGGCGGCTATGTATGCGGCATTCGCGCAGGCC
AGCTTGGCATCGATACGGTG

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MAHFAIKLPDVGEGVAEAELVEWHVKVGDIVREDDLLAAVMTDKATVEIPSSRAGKVIAINGEVGEKIAVGSELVRLEIE
GGATEKAEEPQPQAAEPTAPAAAVPEAPVLLQTPVPEKPAAPKRENAGRPFTGAGPLRPEGEKPLATPSVRLRARDAGID
LRRVRGTGPAGRITHEDLDAFFQMESGAAPALSGYAADTSINEIKVIGLRRKIAERMAEAKRHIPHITIVEEVDVTQLEE
LRNGLNHEKKEGHPRLTLLPFIIRTIVKAVKEQPGLNAHFDDEADIIRQFGGVHVGIATQTPNGLIVPVVRHAESMSVFA
AATELSRVTEAARNGTAKREELTGSTITITSLGPLGAIATTPIINRPEVAIVGINKMAVRPMWDGTQFVPRKMMNLSCSF
DHRVIDGWDAAVFVQKLKSLLETPAMIFVEG

Sequences:

>Translated_431_residues
MAHFAIKLPDVGEGVAEAELVEWHVKVGDIVREDDLLAAVMTDKATVEIPSSRAGKVIAINGEVGEKIAVGSELVRLEIE
GGATEKAEEPQPQAAEPTAPAAAVPEAPVLLQTPVPEKPAAPKRENAGRPFTGAGPLRPEGEKPLATPSVRLRARDAGID
LRRVRGTGPAGRITHEDLDAFFQMESGAAPALSGYAADTSINEIKVIGLRRKIAERMAEAKRHIPHITIVEEVDVTQLEE
LRNGLNHEKKEGHPRLTLLPFIIRTIVKAVKEQPGLNAHFDDEADIIRQFGGVHVGIATQTPNGLIVPVVRHAESMSVFA
AATELSRVTEAARNGTAKREELTGSTITITSLGPLGAIATTPIINRPEVAIVGINKMAVRPMWDGTQFVPRKMMNLSCSF
DHRVIDGWDAAVFVQKLKSLLETPAMIFVEG
>Mature_430_residues
AHFAIKLPDVGEGVAEAELVEWHVKVGDIVREDDLLAAVMTDKATVEIPSSRAGKVIAINGEVGEKIAVGSELVRLEIEG
GATEKAEEPQPQAAEPTAPAAAVPEAPVLLQTPVPEKPAAPKRENAGRPFTGAGPLRPEGEKPLATPSVRLRARDAGIDL
RRVRGTGPAGRITHEDLDAFFQMESGAAPALSGYAADTSINEIKVIGLRRKIAERMAEAKRHIPHITIVEEVDVTQLEEL
RNGLNHEKKEGHPRLTLLPFIIRTIVKAVKEQPGLNAHFDDEADIIRQFGGVHVGIATQTPNGLIVPVVRHAESMSVFAA
ATELSRVTEAARNGTAKREELTGSTITITSLGPLGAIATTPIINRPEVAIVGINKMAVRPMWDGTQFVPRKMMNLSCSFD
HRVIDGWDAAVFVQKLKSLLETPAMIFVEG

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=449, Percent_Identity=29.6213808463252, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI31711992, Length=443, Percent_Identity=29.5711060948081, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI19923748, Length=225, Percent_Identity=31.1111111111111, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI203098753, Length=463, Percent_Identity=24.1900647948164, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI203098816, Length=463, Percent_Identity=24.1900647948164, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI260898739, Length=166, Percent_Identity=25.9036144578313, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1786305, Length=294, Percent_Identity=33.6734693877551, Blast_Score=184, Evalue=7e-48,
Organism=Escherichia coli, GI1786946, Length=439, Percent_Identity=29.6127562642369, Blast_Score=175, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17537937, Length=443, Percent_Identity=32.5056433408578, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI25146366, Length=425, Percent_Identity=29.6470588235294, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17560088, Length=448, Percent_Identity=28.125, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17538894, Length=308, Percent_Identity=26.2987012987013, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320352, Length=427, Percent_Identity=28.1030444964871, Blast_Score=179, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6324258, Length=461, Percent_Identity=27.114967462039, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI18859875, Length=447, Percent_Identity=30.4250559284116, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24645909, Length=225, Percent_Identity=31.5555555555556, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24582497, Length=226, Percent_Identity=28.3185840707965, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI20129315, Length=226, Percent_Identity=28.3185840707965, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 46331; Mature: 46200

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHFAIKLPDVGEGVAEAELVEWHVKVGDIVREDDLLAAVMTDKATVEIPSSRAGKVIAI
CCEEEEECCCCCCCCHHHHHHEEEEHHCCEECCCCEEEEEECCCEEEECCCCCCCEEEEE
NGEVGEKIAVGSELVRLEIEGGATEKAEEPQPQAAEPTAPAAAVPEAPVLLQTPVPEKPA
ECCCCCEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCC
APKRENAGRPFTGAGPLRPEGEKPLATPSVRLRARDAGIDLRRVRGTGPAGRITHEDLDA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCHHHHHH
FFQMESGAAPALSGYAADTSINEIKVIGLRRKIAERMAEAKRHIPHITIVEEVDVTQLEE
HHHHCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHH
LRNGLNHEKKEGHPRLTLLPFIIRTIVKAVKEQPGLNAHFDDEADIIRQFGGVHVGIATQ
HHHCCCCCHHCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCEEEEEEEE
TPNGLIVPVVRHAESMSVFAAATELSRVTEAARNGTAKREELTGSTITITSLGPLGAIAT
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEECCCCCHHHC
TPIINRPEVAIVGINKMAVRPMWDGTQFVPRKMMNLSCSFDHRVIDGWDAAVFVQKLKSL
CCCCCCCCEEEEECCCEEECCCCCCCHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHH
LETPAMIFVEG
HCCCCEEEEEC
>Mature Secondary Structure 
AHFAIKLPDVGEGVAEAELVEWHVKVGDIVREDDLLAAVMTDKATVEIPSSRAGKVIAI
CEEEEECCCCCCCCHHHHHHEEEEHHCCEECCCCEEEEEECCCEEEECCCCCCCEEEEE
NGEVGEKIAVGSELVRLEIEGGATEKAEEPQPQAAEPTAPAAAVPEAPVLLQTPVPEKPA
ECCCCCEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCC
APKRENAGRPFTGAGPLRPEGEKPLATPSVRLRARDAGIDLRRVRGTGPAGRITHEDLDA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCHHHHHH
FFQMESGAAPALSGYAADTSINEIKVIGLRRKIAERMAEAKRHIPHITIVEEVDVTQLEE
HHHHCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHH
LRNGLNHEKKEGHPRLTLLPFIIRTIVKAVKEQPGLNAHFDDEADIIRQFGGVHVGIATQ
HHHCCCCCHHCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCEEEEEEEE
TPNGLIVPVVRHAESMSVFAAATELSRVTEAARNGTAKREELTGSTITITSLGPLGAIAT
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEECCCCCHHHC
TPIINRPEVAIVGINKMAVRPMWDGTQFVPRKMMNLSCSFDHRVIDGWDAAVFVQKLKSL
CCCCCCCCEEEEECCCEEECCCCCCCHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHH
LETPAMIFVEG
HCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3046941 [H]