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The map label for this gene is bigR [H]

Identifier: 225686260

GI number: 225686260

Start: 425899

End: 426222

Strand: Direct

Name: bigR [H]

Synonym: BMEA_B0435

Alternate gene names: 225686260

Gene position: 425899-426222 (Clockwise)

Preceding gene: 225686257

Following gene: 225686261

Centisome position: 35.93

GC content: 54.94

Gene sequence:

>324_bases
ATGGATACCCGGCTTGTTACGCGCGAGCAGATGGAGGCTCGCGCAAACGAGGTTGCAGAACTGCTCAAGACGCTGTCGCA
TCCGGCGCGGCTGATGCTGGCCTGTACGCTGGCCGAAGGGGAATATTCAGTTGGTGAACTGGAAGAAATGCTCGGCATCC
GCCAGCCAACCTTGTCGCAGCAGCTTGGTGTGTTGCGGGAAGCAGATATTGTCGAGACCCGCCGCGAGGCCAAGCAGATT
TACTATCGGCTGGTTGAAACAAAAGCAGCGAAGTTGATTGACGCGCTTTATGGAATTTTCTGCAAGGAAGGAACGGATTC
ATGA

Upstream 100 bases:

>100_bases
TGCTTGAATTTATAAAAACATATAATATTTAAATATATAATGATGCCATGGCAGGGGCGGCTGTCCGGTTTTGTCTCAAG
CTTCTGGATCGAGGTTGGTT

Downstream 100 bases:

>100_bases
TGGCGCTCTATATGCAGGCGCTTCTCGGCGGCATGTTGATCGGGCTTTCCGCAGTGCTGCTTCTGCTTCTTAACGGCAGG
GTCGCCGGGATCAGCGGCAT

Product: regulatory protein ArsR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 107; Mature: 107

Protein sequence:

>107_residues
MDTRLVTREQMEARANEVAELLKTLSHPARLMLACTLAEGEYSVGELEEMLGIRQPTLSQQLGVLREADIVETRREAKQI
YYRLVETKAAKLIDALYGIFCKEGTDS

Sequences:

>Translated_107_residues
MDTRLVTREQMEARANEVAELLKTLSHPARLMLACTLAEGEYSVGELEEMLGIRQPTLSQQLGVLREADIVETRREAKQI
YYRLVETKAAKLIDALYGIFCKEGTDS
>Mature_107_residues
MDTRLVTREQMEARANEVAELLKTLSHPARLMLACTLAEGEYSVGELEEMLGIRQPTLSQQLGVLREADIVETRREAKQI
YYRLVETKAAKLIDALYGIFCKEGTDS

Specific function: Represses an operon that comprises itself, XF_0764, XF_0765, XF_0766 and blh. Binds to a palindromic AT-rich sequence spanning the -10 region of the blh promoter and blocks transcription of the operon [H]

COG id: COG0640

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH arsR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789021, Length=93, Percent_Identity=32.258064516129, Blast_Score=62, Evalue=8e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001845
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01022 HTH_5 [H]

EC number: NA

Molecular weight: Translated: 12089; Mature: 12089

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS50987 HTH_ARSR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTRLVTREQMEARANEVAELLKTLSHPARLMLACTLAEGEYSVGELEEMLGIRQPTLSQ
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHH
QLGVLREADIVETRREAKQIYYRLVETKAAKLIDALYGIFCKEGTDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDTRLVTREQMEARANEVAELLKTLSHPARLMLACTLAEGEYSVGELEEMLGIRQPTLSQ
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHH
QLGVLREADIVETRREAKQIYYRLVETKAAKLIDALYGIFCKEGTDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]