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The map label for this gene is rfbF [H]

Identifier: 225686247

GI number: 225686247

Start: 408444

End: 409253

Strand: Direct

Name: rfbF [H]

Synonym: BMEA_B0418

Alternate gene names: 225686247

Gene position: 408444-409253 (Clockwise)

Preceding gene: 225686246

Following gene: 225686248

Centisome position: 34.45

GC content: 53.83

Gene sequence:

>810_bases
ATGAAAGTCGTTCTGTTCTGCGGTGGTCTTGGCACGCGAATCCGCGAATATTCGGAAAATATTCCAAAGCCCATGATCCC
GCTCGGTCATCAGCCGATCCTGCATCATGTCATGGAATATTACAGCGATTATGGCCACAATGATTTCGTCCTGTGCCTTG
GATACAAGGCGAATGTCGTCAAGGATTTCTTCCTCACTATTCGCCCGCAGACCTTCGCAGATTGCGTGGTTTCGGATGGC
GGGCGCAATGTGCAGCTTCTGGAGGAGGTGGATCGCGACTGGAGCGTCACACTGCTTGACACCGGCATCTGGCGCAATAT
TGGTGAGCGGCTATGGAGTGCACGTTCCCACGTAATGAACGAGGATATATTTCTCGCCAATTACAGTGACGGCCTCAGCG
ATGTCGATCTTGATAATATGACGCAGCGGTTTCGCGCCAGCGGCAAGGTTGCCTGTTTCCTCGCGGTGCGCCCGCCTTTG
ACCTATCATCTGGCCGATATTGCCGAGGGCGGCGATGTGCGCGCGTTTCGTACCTCCAATACGTCCGATATCTGGATCAA
TGGCGGCTATTTCCTCTTCCTCAAGGAAATCTTCGACTATATGCGCGAAGGCGAGGAACTGGTGCTGGAACCGTTCAGCC
GCCTGATCGCCGAAAACCAGTTGATGGCCTATAAATACGACGGATTCTGGCGGTCTATGGATACGCTGCGCGACTGGCAG
ACGCTGGAGGATATGGTGGAGAAAGGCGATATGCCCTGGAAGAAACACAAGGCGGAACAGCGGGCCGCCAATATTGCGAT
AGCACTATGA

Upstream 100 bases:

>100_bases
GGCAGATGCAGCATGTCGTGGAATGGGGCGCGAAATTCATCATTCCCATTCCCCATGTCACCTTGATCGATCCGGCACTC
GTTACGGAGGAGCGCTAATC

Downstream 100 bases:

>100_bases
TCGACCTGTGCGCGCTGGCAAATCCGGGCCGTCCGCTTGAACTTTTGTGCCTTGGCGCACATTCCGACGATATCGAGATC
GGTTGCGGCGGTACGCTTCT

Product: glucose-1-phosphate cytidylyltransferase

Products: NA

Alternate protein names: CDP-glucose pyrophosphorylase [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MKVVLFCGGLGTRIREYSENIPKPMIPLGHQPILHHVMEYYSDYGHNDFVLCLGYKANVVKDFFLTIRPQTFADCVVSDG
GRNVQLLEEVDRDWSVTLLDTGIWRNIGERLWSARSHVMNEDIFLANYSDGLSDVDLDNMTQRFRASGKVACFLAVRPPL
TYHLADIAEGGDVRAFRTSNTSDIWINGGYFLFLKEIFDYMREGEELVLEPFSRLIAENQLMAYKYDGFWRSMDTLRDWQ
TLEDMVEKGDMPWKKHKAEQRAANIAIAL

Sequences:

>Translated_269_residues
MKVVLFCGGLGTRIREYSENIPKPMIPLGHQPILHHVMEYYSDYGHNDFVLCLGYKANVVKDFFLTIRPQTFADCVVSDG
GRNVQLLEEVDRDWSVTLLDTGIWRNIGERLWSARSHVMNEDIFLANYSDGLSDVDLDNMTQRFRASGKVACFLAVRPPL
TYHLADIAEGGDVRAFRTSNTSDIWINGGYFLFLKEIFDYMREGEELVLEPFSRLIAENQLMAYKYDGFWRSMDTLRDWQ
TLEDMVEKGDMPWKKHKAEQRAANIAIAL
>Mature_269_residues
MKVVLFCGGLGTRIREYSENIPKPMIPLGHQPILHHVMEYYSDYGHNDFVLCLGYKANVVKDFFLTIRPQTFADCVVSDG
GRNVQLLEEVDRDWSVTLLDTGIWRNIGERLWSARSHVMNEDIFLANYSDGLSDVDLDNMTQRFRASGKVACFLAVRPPL
TYHLADIAEGGDVRAFRTSNTSDIWINGGYFLFLKEIFDYMREGEELVLEPFSRLIAENQLMAYKYDGFWRSMDTLRDWQ
TLEDMVEKGDMPWKKHKAEQRAANIAIAL

Specific function: Involved in the biosynthesis of the tyvelose, a 3,6- dideoxyhexose found in the O-antigen of the surface lipopolysaccharides. It catalyzes the transfer of a CMP moiety from CTP to glucose 1-phosphate. This enzyme can utilize either CTP or UTP as the nucle

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate cytidylyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013446
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.33 [H]

Molecular weight: Translated: 31024; Mature: 31024

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVLFCGGLGTRIREYSENIPKPMIPLGHQPILHHVMEYYSDYGHNDFVLCLGYKANVV
CEEEEEECCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHH
KDFFLTIRPQTFADCVVSDGGRNVQLLEEVDRDWSVTLLDTGIWRNIGERLWSARSHVMN
HHHHHEECCCHHHHHHHCCCCCCCHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHCC
EDIFLANYSDGLSDVDLDNMTQRFRASGKVACFLAVRPPLTYHLADIAEGGDVRAFRTSN
CCEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCEEHHHHHCCCCCEEEEECCC
TSDIWINGGYFLFLKEIFDYMREGEELVLEPFSRLIAENQLMAYKYDGFWRSMDTLRDWQ
CCEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHH
TLEDMVEKGDMPWKKHKAEQRAANIAIAL
HHHHHHHHCCCCHHHHHHHHHHHCEEEEC
>Mature Secondary Structure
MKVVLFCGGLGTRIREYSENIPKPMIPLGHQPILHHVMEYYSDYGHNDFVLCLGYKANVV
CEEEEEECCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHH
KDFFLTIRPQTFADCVVSDGGRNVQLLEEVDRDWSVTLLDTGIWRNIGERLWSARSHVMN
HHHHHEECCCHHHHHHHCCCCCCCHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHCC
EDIFLANYSDGLSDVDLDNMTQRFRASGKVACFLAVRPPLTYHLADIAEGGDVRAFRTSN
CCEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCEEHHHHHCCCCCEEEEECCC
TSDIWINGGYFLFLKEIFDYMREGEELVLEPFSRLIAENQLMAYKYDGFWRSMDTLRDWQ
CCEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHH
TLEDMVEKGDMPWKKHKAEQRAANIAIAL
HHHHHHHHCCCCHHHHHHHHHHHCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]