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The map label for this gene is livK [C]

Identifier: 225686207

GI number: 225686207

Start: 353872

End: 355083

Strand: Direct

Name: livK [C]

Synonym: BMEA_B0373

Alternate gene names: 225686207

Gene position: 353872-355083 (Clockwise)

Preceding gene: 225686206

Following gene: 225686208

Centisome position: 29.85

GC content: 56.85

Gene sequence:

>1212_bases
ATGAGACTGTCCAGGCTCCTTATCGGCGCATCGCTCGGCGTTGCGCTTTCGTCCACTGTCTTCACCGCGGCACTCGCCGA
TGTGAAATTCGGCTCGCTTTATCCGATCAGCGGTTCGCTTGCGCTTCTGGGTGAAGAAAGCGCACGCGGTCTGGAACTTG
CCGTAGACGAGGTCAATGCGGCTGGCGGCATCAAGGGTGAAAAAGTCGTGCTTGAGCGCGGCGATGCCGTTGACAACAAC
CAGGCTACCGGTGAGGCTCGCCGCCTGATCTCGCTTGTGGGTGTGAAGGCGATTTTCGGGACTTATTCTTCGGCGCGGGT
TATTGCCGCCTCGCAGGTGAGCGAGCTTGCAGGCATACCCTATTTCGAAATGGGTGCCGTTGCCGACGAGGTGACGGGGC
GTGGCTTGCAATATCTTTTCCGCACCAATCCGACCGCGGAAAACATGGCCAAGGACTCTGTAGATATGCTCATCAAGGGC
ATTGCTCCGGGCCTCGGCAAAGACCCCAAGGATATGAAAATCGGCATTATTTATGAGGACTCTTCCTATGGCACATCCGT
TGCCGGGCATCAGGAAGATAACGCAAAGGCAGCCGGTCTCACTGTCGTTCTGAAATCCGGCTATCCATCCAACACAGTCG
ACATGTCTTCGCTGGTTCTGGAGCTGCGCGAGAAGGGTGCGGATGTCGTCATGCAGACGTCCTACCAGAACGATTCCGTC
CTGTTCCTGCAACAGGCCAACGAAGCGGGCTATAAGCCCCTTGCCATCATCGGCGGTGGCGGCGGTTATTCCATGCAGCC
AACGGCGGACGCCGTGGGACATGACCTGATCGACGGCGTTTTCGATGCCGACTATACGCAATATGCAGTCAACACCTCCG
CCACGCCGGGCCTGACCGAATTTGTGGAAGCCTATAAGGCCAAATATGGCTCGCAACCGCGTTCCGGCCATTCGCTGACC
AATTATGTCGGGGCGAAGGCGATTTTTCAGGCTTTGAACGCTGGGGAAGGCTTTGAACCGGACCAGATCGTAAGCGCAGT
CAAGGCACTGGACATTCCGGACGGCCAGACGGCAGCAGGCTATGGGGTCAAGTTTGGCAAGAACAACCAGAATGAGCGTG
CGACGATGATGGGGATGCAGTGGCAGGATGGCAAGCTCGTTACCGTCTATCCGGAAAATGCCGCTATCGCCAAGATGCGC
TTCAAGAAATGA

Upstream 100 bases:

>100_bases
CTTGTGATGCAGCGTGACGCGAGGTTGGGCGGGCTGCGCGCAATACGGCTCAATGCGGAAAGCGGCCCGAAACACAAATA
ATTCTTTGAGGGAGAAAAGA

Downstream 100 bases:

>100_bases
TCCTGTGTGGGCGGGCGGCCGTTGTGCTGCCCAGCTCGGGGAGCTGATATGACTACCTTTCTGCAAATTCTTCTGAACGG
CCTGATGCTCGGCGGGCTAT

Product: Leu/Ile/Val-binding protein family protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MRLSRLLIGASLGVALSSTVFTAALADVKFGSLYPISGSLALLGEESARGLELAVDEVNAAGGIKGEKVVLERGDAVDNN
QATGEARRLISLVGVKAIFGTYSSARVIAASQVSELAGIPYFEMGAVADEVTGRGLQYLFRTNPTAENMAKDSVDMLIKG
IAPGLGKDPKDMKIGIIYEDSSYGTSVAGHQEDNAKAAGLTVVLKSGYPSNTVDMSSLVLELREKGADVVMQTSYQNDSV
LFLQQANEAGYKPLAIIGGGGGYSMQPTADAVGHDLIDGVFDADYTQYAVNTSATPGLTEFVEAYKAKYGSQPRSGHSLT
NYVGAKAIFQALNAGEGFEPDQIVSAVKALDIPDGQTAAGYGVKFGKNNQNERATMMGMQWQDGKLVTVYPENAAIAKMR
FKK

Sequences:

>Translated_403_residues
MRLSRLLIGASLGVALSSTVFTAALADVKFGSLYPISGSLALLGEESARGLELAVDEVNAAGGIKGEKVVLERGDAVDNN
QATGEARRLISLVGVKAIFGTYSSARVIAASQVSELAGIPYFEMGAVADEVTGRGLQYLFRTNPTAENMAKDSVDMLIKG
IAPGLGKDPKDMKIGIIYEDSSYGTSVAGHQEDNAKAAGLTVVLKSGYPSNTVDMSSLVLELREKGADVVMQTSYQNDSV
LFLQQANEAGYKPLAIIGGGGGYSMQPTADAVGHDLIDGVFDADYTQYAVNTSATPGLTEFVEAYKAKYGSQPRSGHSLT
NYVGAKAIFQALNAGEGFEPDQIVSAVKALDIPDGQTAAGYGVKFGKNNQNERATMMGMQWQDGKLVTVYPENAAIAKMR
FKK
>Mature_403_residues
MRLSRLLIGASLGVALSSTVFTAALADVKFGSLYPISGSLALLGEESARGLELAVDEVNAAGGIKGEKVVLERGDAVDNN
QATGEARRLISLVGVKAIFGTYSSARVIAASQVSELAGIPYFEMGAVADEVTGRGLQYLFRTNPTAENMAKDSVDMLIKG
IAPGLGKDPKDMKIGIIYEDSSYGTSVAGHQEDNAKAAGLTVVLKSGYPSNTVDMSSLVLELREKGADVVMQTSYQNDSV
LFLQQANEAGYKPLAIIGGGGGYSMQPTADAVGHDLIDGVFDADYTQYAVNTSATPGLTEFVEAYKAKYGSQPRSGHSLT
NYVGAKAIFQALNAGEGFEPDQIVSAVKALDIPDGQTAAGYGVKFGKNNQNERATMMGMQWQDGKLVTVYPENAAIAKMR
FKK

Specific function: Component of an amino-acid transport system (Potential)

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family

Homologues:

Organism=Escherichia coli, GI1789867, Length=252, Percent_Identity=29.3650793650794, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI48994941, Length=250, Percent_Identity=29.2, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LIVB8_BRUA2 (Q2YK50)

Other databases:

- EMBL:   AM040265
- RefSeq:   YP_418984.1
- ProteinModelPortal:   Q2YK50
- STRING:   Q2YK50
- GeneID:   3827100
- GenomeReviews:   AM040265_GR
- KEGG:   bmf:BAB2_0830
- eggNOG:   COG0683
- HOGENOM:   HBG754289
- OMA:   FGALYPF
- ProtClustDB:   CLSK898977
- BioCyc:   BMEL359391:BAB2_0830-MONOMER
- InterPro:   IPR001828
- InterPro:   IPR000709
- PRINTS:   PR00337

Pfam domain/function: PF01094 ANF_receptor

EC number: NA

Molecular weight: Translated: 42457; Mature: 42457

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSRLLIGASLGVALSSTVFTAALADVKFGSLYPISGSLALLGEESARGLELAVDEVNA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEEECCCCCCEEEEEEHHHC
AGGIKGEKVVLERGDAVDNNQATGEARRLISLVGVKAIFGTYSSARVIAASQVSELAGIP
CCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHCCC
YFEMGAVADEVTGRGLQYLFRTNPTAENMAKDSVDMLIKGIAPGLGKDPKDMKIGIIYED
CHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEC
SSYGTSVAGHQEDNAKAAGLTVVLKSGYPSNTVDMSSLVLELREKGADVVMQTSYQNDSV
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCE
LFLQQANEAGYKPLAIIGGGGGYSMQPTADAVGHDLIDGVFDADYTQYAVNTSATPGLTE
EEEEECCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHH
FVEAYKAKYGSQPRSGHSLTNYVGAKAIFQALNAGEGFEPDQIVSAVKALDIPDGQTAAG
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCC
YGVKFGKNNQNERATMMGMQWQDGKLVTVYPENAAIAKMRFKK
CCEEECCCCCCCCEEEECEEECCCEEEEEECCCCEEEEEECCC
>Mature Secondary Structure
MRLSRLLIGASLGVALSSTVFTAALADVKFGSLYPISGSLALLGEESARGLELAVDEVNA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEEECCCCCCEEEEEEHHHC
AGGIKGEKVVLERGDAVDNNQATGEARRLISLVGVKAIFGTYSSARVIAASQVSELAGIP
CCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHCCC
YFEMGAVADEVTGRGLQYLFRTNPTAENMAKDSVDMLIKGIAPGLGKDPKDMKIGIIYED
CHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEC
SSYGTSVAGHQEDNAKAAGLTVVLKSGYPSNTVDMSSLVLELREKGADVVMQTSYQNDSV
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCE
LFLQQANEAGYKPLAIIGGGGGYSMQPTADAVGHDLIDGVFDADYTQYAVNTSATPGLTE
EEEEECCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHH
FVEAYKAKYGSQPRSGHSLTNYVGAKAIFQALNAGEGFEPDQIVSAVKALDIPDGQTAAG
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCC
YGVKFGKNNQNERATMMGMQWQDGKLVTVYPENAAIAKMRFKK
CCEEECCCCCCCCEEEECEEECCCEEEEEECCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA