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The map label for this gene is potH [H]

Identifier: 225686167

GI number: 225686167

Start: 307548

End: 308786

Strand: Direct

Name: potH [H]

Synonym: BMEA_B0328

Alternate gene names: 225686167

Gene position: 307548-308786 (Clockwise)

Preceding gene: 225686166

Following gene: 225686168

Centisome position: 25.94

GC content: 59.48

Gene sequence:

>1239_bases
ATGGCAGCCGGGTGCGGACGTGGCCGCAAGCTTCAAGGCCGCCGATTGCTGGACCATCCTCGTATGACGGCGCCCTCATC
CAGAACGCTCCGCGCAGGGCTTTTGGTTACCCCATTGCTCATCTTCATCGTCGTGTTTTTCCTGTCGCCGCTTGGCGTGA
TGATGAAGCAGGCGGTATCCGACACGGCTGTCCTGCATGTGCTGCCGCAAACGGCGGAAGCCATCAGCCAATGGGATGGC
CAGTCTGGTCCGACCCCGGCGATGAAAGAGGCGCTGGTGGCGGATTTGAAAGGCTCTTCCGATCAGCAGGCCATTGGTGA
CATGGTGCGCAGGCTCAACAGCGCCCAGGCGGGTTTCAGAACCTTGATGAGCAAGACCTTGAGAGCCATCGACAAGGAGG
GGCCGCCGCCTGATCTGGAAGCGGTGGACAAGCGGTGGAGCAGGCCGGAGTTCTGGCTGGCTATTGCCAATGCCCTGTCA
CCCCTGACGGATCGCAATCTTCTGGCGGCGGTGGATATGGAGCGCGACACTTCCGGTGAAATCGTCTCCATGGCGCCCGG
ATCTTCGGCCAACCGCGCAATCATGATCCGCACATTCTGGATTGCAGCACTCGTCACGCTTGCTTGTGTGGCTATAGGCT
ATCCTTATGCCGTCCTGCTGGTTTCGTCCTCCGGATGGGTGAGAAACGTGCTTTTTGCCGCTGTTTTGTTGCCGCTCTGG
ACCTCGTTGCTGGTTAGAACCGCCGCCTGGTTCATTCTCTTGCAGGACAAGGGCCTCATCAATGACAGCCTGATCTGGCT
CGGAATAACAGACAGGCCTTTTCCGCTCATCTTCAACCGGACGGGTGTTGTCATTGCCATGACGCATGTGCTTTTGCCCT
TCATGGTGCTGCCGATCTATAGTGTGCTGGTTTCCATTCCCGGCAACCTCATGCCTGCGGCCGCGTCGCTTGGGGCGCCG
CCATGGCGGGCTTTCCTGCGCGTCCTGCTGCCGCTCAGCATGCGCGGCATCCTGTCGGGTATGCTGCTCGTCTTCATGAG
CGCCATCGGTTATTATATTACACCTGCGCTGATTGGCGGACCGGGCGACCAGATGATCAGCTCGATCATCGCCTATTATG
CTACAGGTTCGGCGAACTGGGGCATGGCCGGTGCTTTGGGGCTTGTGCTGCTCGTCGCCTGTCTCATCCTTTATGCCGTT
TACGGACGCTTGACCGCCGATGACGAGGGGAGGGCGTGA

Upstream 100 bases:

>100_bases
CGCGCGTGATGGACAGTGTTTATCAGGGCGACCATCTGCGTATCCAGCTTGAGGCCAAGGCGCATAATTTTGTGGTTCGC
CTTGATCGCAAGGCGCGCGA

Downstream 100 bases:

>100_bases
CAATGGGGCGTGTTGCAAAAATTCTTTTCGGTGTTGCGGCAGTCGTTTTTCTGGTCGCACCGCTTTTCGCCATCCTGCCG
CTCGCCTTCACCTCCAGCGT

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MAAGCGRGRKLQGRRLLDHPRMTAPSSRTLRAGLLVTPLLIFIVVFFLSPLGVMMKQAVSDTAVLHVLPQTAEAISQWDG
QSGPTPAMKEALVADLKGSSDQQAIGDMVRRLNSAQAGFRTLMSKTLRAIDKEGPPPDLEAVDKRWSRPEFWLAIANALS
PLTDRNLLAAVDMERDTSGEIVSMAPGSSANRAIMIRTFWIAALVTLACVAIGYPYAVLLVSSSGWVRNVLFAAVLLPLW
TSLLVRTAAWFILLQDKGLINDSLIWLGITDRPFPLIFNRTGVVIAMTHVLLPFMVLPIYSVLVSIPGNLMPAAASLGAP
PWRAFLRVLLPLSMRGILSGMLLVFMSAIGYYITPALIGGPGDQMISSIIAYYATGSANWGMAGALGLVLLVACLILYAV
YGRLTADDEGRA

Sequences:

>Translated_412_residues
MAAGCGRGRKLQGRRLLDHPRMTAPSSRTLRAGLLVTPLLIFIVVFFLSPLGVMMKQAVSDTAVLHVLPQTAEAISQWDG
QSGPTPAMKEALVADLKGSSDQQAIGDMVRRLNSAQAGFRTLMSKTLRAIDKEGPPPDLEAVDKRWSRPEFWLAIANALS
PLTDRNLLAAVDMERDTSGEIVSMAPGSSANRAIMIRTFWIAALVTLACVAIGYPYAVLLVSSSGWVRNVLFAAVLLPLW
TSLLVRTAAWFILLQDKGLINDSLIWLGITDRPFPLIFNRTGVVIAMTHVLLPFMVLPIYSVLVSIPGNLMPAAASLGAP
PWRAFLRVLLPLSMRGILSGMLLVFMSAIGYYITPALIGGPGDQMISSIIAYYATGSANWGMAGALGLVLLVACLILYAV
YGRLTADDEGRA
>Mature_411_residues
AAGCGRGRKLQGRRLLDHPRMTAPSSRTLRAGLLVTPLLIFIVVFFLSPLGVMMKQAVSDTAVLHVLPQTAEAISQWDGQ
SGPTPAMKEALVADLKGSSDQQAIGDMVRRLNSAQAGFRTLMSKTLRAIDKEGPPPDLEAVDKRWSRPEFWLAIANALSP
LTDRNLLAAVDMERDTSGEIVSMAPGSSANRAIMIRTFWIAALVTLACVAIGYPYAVLLVSSSGWVRNVLFAAVLLPLWT
SLLVRTAAWFILLQDKGLINDSLIWLGITDRPFPLIFNRTGVVIAMTHVLLPFMVLPIYSVLVSIPGNLMPAAASLGAPP
WRAFLRVLLPLSMRGILSGMLLVFMSAIGYYITPALIGGPGDQMISSIIAYYATGSANWGMAGALGLVLLVACLILYAVY
GRLTADDEGRA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=201, Percent_Identity=36.81592039801, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1787369, Length=210, Percent_Identity=33.8095238095238, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1787713, Length=233, Percent_Identity=31.7596566523605, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 44358; Mature: 44227

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAGCGRGRKLQGRRLLDHPRMTAPSSRTLRAGLLVTPLLIFIVVFFLSPLGVMMKQAVS
CCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DTAVLHVLPQTAEAISQWDGQSGPTPAMKEALVADLKGSSDQQAIGDMVRRLNSAQAGFR
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
TLMSKTLRAIDKEGPPPDLEAVDKRWSRPEFWLAIANALSPLTDRNLLAAVDMERDTSGE
HHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCC
IVSMAPGSSANRAIMIRTFWIAALVTLACVAIGYPYAVLLVSSSGWVRNVLFAAVLLPLW
EEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHH
TSLLVRTAAWFILLQDKGLINDSLIWLGITDRPFPLIFNRTGVVIAMTHVLLPFMVLPIY
HHHHHHHHHHHEEEECCCCCCCCEEEEEECCCCCCEEECCCCHHHHHHHHHHHHHHHHHH
SVLVSIPGNLMPAAASLGAPPWRAFLRVLLPLSMRGILSGMLLVFMSAIGYYITPALIGG
HHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PGDQMISSIIAYYATGSANWGMAGALGLVLLVACLILYAVYGRLTADDEGRA
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
AAGCGRGRKLQGRRLLDHPRMTAPSSRTLRAGLLVTPLLIFIVVFFLSPLGVMMKQAVS
CCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DTAVLHVLPQTAEAISQWDGQSGPTPAMKEALVADLKGSSDQQAIGDMVRRLNSAQAGFR
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
TLMSKTLRAIDKEGPPPDLEAVDKRWSRPEFWLAIANALSPLTDRNLLAAVDMERDTSGE
HHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCC
IVSMAPGSSANRAIMIRTFWIAALVTLACVAIGYPYAVLLVSSSGWVRNVLFAAVLLPLW
EEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHH
TSLLVRTAAWFILLQDKGLINDSLIWLGITDRPFPLIFNRTGVVIAMTHVLLPFMVLPIY
HHHHHHHHHHHEEEECCCCCCCCEEEEEECCCCCCEEECCCCHHHHHHHHHHHHHHHHHH
SVLVSIPGNLMPAAASLGAPPWRAFLRVLLPLSMRGILSGMLLVFMSAIGYYITPALIGG
HHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PGDQMISSIIAYYATGSANWGMAGALGLVLLVACLILYAVYGRLTADDEGRA
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]