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The map label for this gene is hisH [H]

Identifier: 225853514

GI number: 225853514

Start: 2024726

End: 2025376

Strand: Direct

Name: hisH [H]

Synonym: BMEA_A2145

Alternate gene names: 225853514

Gene position: 2024726-2025376 (Clockwise)

Preceding gene: 225853513

Following gene: 225853515

Centisome position: 95.25

GC content: 57.6

Gene sequence:

>651_bases
ATGCGTGTTGCTATTATTGACTATGGTTCCGGCAATCTTCGCTCGGCCACCAAGGCTTTCGAGCGCGCCGCCCATGAAAG
CGGCATATCGGCGGAAATCGACCTGACTTGCGATGCGCAGCGGGTTGCCAGCGCAGACCGCATCGTCCTGCCCGGTGTTG
GCGCCTATGCGGATTGCCGCCGTGGGCTCGATGCCGTACCCGGCATGGTCGAGGCACTGAACGATACCGTATTGAAGAAA
GCGCGTCCGTTCCTTGGCATCTGCGTGGGTATGCAGCTTATGTCGGAACGCGGACTGGAAAAAACCGTCACGAACGGCCT
TGGCTGGATTGCGGGCGATGTGCGTGAAATGGTGCCGTCCGATGCATCGCTGAAAATTCCGCAGATCGGCTGGAATCGGA
TTCACGTGAAACATTCGCATCCGATCTTCGACGGAATCCCCACCGGCGACGACGGCCTGCATGCCTATTTTGTCCACTCC
TATATGCTGGATGCGAAAAATGCATCCGATGTTCTGGCTGTGACCGATTATGGCGGCGACGTTACCGCCGCCGTCGGGCG
CGACAACATGGTGGGCACCCAGTTCCATCCCGAAAAGAGCCAGTTGCTCGGCCTTTCGTTCATTGCCAATTTCCTGAAAT
GGAAGCCTTGA

Upstream 100 bases:

>100_bases
ATATCCCCGTTCCGCAATGGGGCCAGCAAGTGTCCCGCCCTGCTCCTTCCACCTTTGGCGCGACTATAGGATTTGTCGGC
CATCGCGGAGAAAACTGAAA

Downstream 100 bases:

>100_bases
AATCATGATCCTTTTTCCCGCCATCGATTTGAAAGACGGTCAATGCGTGCGCCTCAAGCTCGGCGACATGGATCAGGCGA
CCATCTACAACGAAGACCCC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MRVAIIDYGSGNLRSATKAFERAAHESGISAEIDLTCDAQRVASADRIVLPGVGAYADCRRGLDAVPGMVEALNDTVLKK
ARPFLGICVGMQLMSERGLEKTVTNGLGWIAGDVREMVPSDASLKIPQIGWNRIHVKHSHPIFDGIPTGDDGLHAYFVHS
YMLDAKNASDVLAVTDYGGDVTAAVGRDNMVGTQFHPEKSQLLGLSFIANFLKWKP

Sequences:

>Translated_216_residues
MRVAIIDYGSGNLRSATKAFERAAHESGISAEIDLTCDAQRVASADRIVLPGVGAYADCRRGLDAVPGMVEALNDTVLKK
ARPFLGICVGMQLMSERGLEKTVTNGLGWIAGDVREMVPSDASLKIPQIGWNRIHVKHSHPIFDGIPTGDDGLHAYFVHS
YMLDAKNASDVLAVTDYGGDVTAAVGRDNMVGTQFHPEKSQLLGLSFIANFLKWKP
>Mature_216_residues
MRVAIIDYGSGNLRSATKAFERAAHESGISAEIDLTCDAQRVASADRIVLPGVGAYADCRRGLDAVPGMVEALNDTVLKK
ARPFLGICVGMQLMSERGLEKTVTNGLGWIAGDVREMVPSDASLKIPQIGWNRIHVKHSHPIFDGIPTGDDGLHAYFVHS
YMLDAKNASDVLAVTDYGGDVTAAVGRDNMVGTQFHPEKSQLLGLSFIANFLKWKP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=213, Percent_Identity=37.5586854460094, Blast_Score=129, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6319725, Length=222, Percent_Identity=31.981981981982, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 23294; Mature: 23294

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVAIIDYGSGNLRSATKAFERAAHESGISAEIDLTCDAQRVASADRIVLPGVGAYADCR
CEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEECHHHHCCCCEEEECCCCCHHHHH
RGLDAVPGMVEALNDTVLKKARPFLGICVGMQLMSERGLEKTVTNGLGWIAGDVREMVPS
CCHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHCCC
DASLKIPQIGWNRIHVKHSHPIFDGIPTGDDGLHAYFVHSYMLDAKNASDVLAVTDYGGD
CCCEECCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCC
VTAAVGRDNMVGTQFHPEKSQLLGLSFIANFLKWKP
EEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRVAIIDYGSGNLRSATKAFERAAHESGISAEIDLTCDAQRVASADRIVLPGVGAYADCR
CEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEECHHHHCCCCEEEECCCCCHHHHH
RGLDAVPGMVEALNDTVLKKARPFLGICVGMQLMSERGLEKTVTNGLGWIAGDVREMVPS
CCHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHCCC
DASLKIPQIGWNRIHVKHSHPIFDGIPTGDDGLHAYFVHSYMLDAKNASDVLAVTDYGGD
CCCEECCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCC
VTAAVGRDNMVGTQFHPEKSQLLGLSFIANFLKWKP
EEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA