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The map label for this gene is hisA

Identifier: 225853515

GI number: 225853515

Start: 2025381

End: 2026112

Strand: Direct

Name: hisA

Synonym: BMEA_A2146

Alternate gene names: 225853515

Gene position: 2025381-2026112 (Clockwise)

Preceding gene: 225853514

Following gene: 225853516

Centisome position: 95.28

GC content: 59.84

Gene sequence:

>732_bases
ATGATCCTTTTTCCCGCCATCGATTTGAAAGACGGTCAATGCGTGCGCCTCAAGCTCGGCGACATGGATCAGGCGACCAT
CTACAACGAAGACCCCGCCGCGCAGGCAAAAGCCTTTGAGGATCAGGGCTTTGAATGGCTGCATGTGGTCGATCTCAACG
GCGCCTTCGCAGGCGAGAGCGTCAATGGCACAGCTGTTGAAGCCATTTTGAAGGCGACGAAGAACCCGGTGCAGCTTGGC
GGCGGCATCCGCACGCTCGCCCATATTGAAAACTGGCTTTCCAGGGGCCTGCGCCGCGTCATTCTGGGGACTGTCGCCGT
GCGCGATCCAGCCCTCGTCATGGAAGCCTGCAAGGCCTTTCCAGGGCAGGTCGCCGTTGGCATCGATGCCAAGGGCGGCA
AGGTTGCCGTGGAAGGCTGGGCCGAAGCTTCAAGGCTGGGCGTTATCGAACTTGCGAAGAAATTTGAAGGCGCAGGCGTC
GCAGCCATCATTTATACCGATATCGACCGTGATGGCGTGCTGGCGGGGATCAACTGGGATTCGACGCTGGCGCTTGCCGA
AGCCGTTTCCATTCCGGTGATTGCCTCCGGCGGCCTTGCCTCGATGGAGGATATCAGGCGTCTGGCCACCCCTGAAATGC
GCAAGCTTGAAGGGGCGATTTCCGGCCGTGCGCTTTACGATGGCCGCATCGACCCGGCGGAAGCATTATCAGTATTGAGG
GCTGCGGCATGA

Upstream 100 bases:

>100_bases
GGGCGCGACAACATGGTGGGCACCCAGTTCCATCCCGAAAAGAGCCAGTTGCTCGGCCTTTCGTTCATTGCCAATTTCCT
GAAATGGAAGCCTTGAAATC

Downstream 100 bases:

>100_bases
CTTTGAAAGCAAGAGTGATTCCCTGTCTCGATGTGAAAGATGGCCGCGTCGTCAAAGGTGTGAACTTCGTTGATCTGATC
GATGCCGGTGATCCGGTGGA

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MILFPAIDLKDGQCVRLKLGDMDQATIYNEDPAAQAKAFEDQGFEWLHVVDLNGAFAGESVNGTAVEAILKATKNPVQLG
GGIRTLAHIENWLSRGLRRVILGTVAVRDPALVMEACKAFPGQVAVGIDAKGGKVAVEGWAEASRLGVIELAKKFEGAGV
AAIIYTDIDRDGVLAGINWDSTLALAEAVSIPVIASGGLASMEDIRRLATPEMRKLEGAISGRALYDGRIDPAEALSVLR
AAA

Sequences:

>Translated_243_residues
MILFPAIDLKDGQCVRLKLGDMDQATIYNEDPAAQAKAFEDQGFEWLHVVDLNGAFAGESVNGTAVEAILKATKNPVQLG
GGIRTLAHIENWLSRGLRRVILGTVAVRDPALVMEACKAFPGQVAVGIDAKGGKVAVEGWAEASRLGVIELAKKFEGAGV
AAIIYTDIDRDGVLAGINWDSTLALAEAVSIPVIASGGLASMEDIRRLATPEMRKLEGAISGRALYDGRIDPAEALSVLR
AAA
>Mature_243_residues
MILFPAIDLKDGQCVRLKLGDMDQATIYNEDPAAQAKAFEDQGFEWLHVVDLNGAFAGESVNGTAVEAILKATKNPVQLG
GGIRTLAHIENWLSRGLRRVILGTVAVRDPALVMEACKAFPGQVAVGIDAKGGKVAVEGWAEASRLGVIELAKKFEGAGV
AAIIYTDIDRDGVLAGINWDSTLALAEAVSIPVIASGGLASMEDIRRLATPEMRKLEGAISGRALYDGRIDPAEALSVLR
AAA

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=252, Percent_Identity=33.7301587301587, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1788336, Length=212, Percent_Identity=26.4150943396226, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_BRUA1 (B2S983)

Other databases:

- EMBL:   CP000887
- RefSeq:   YP_001935909.1
- ProteinModelPortal:   B2S983
- SMR:   B2S983
- GeneID:   6327057
- GenomeReviews:   CP000887_GR
- KEGG:   bmc:BAbS19_I19530
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- ProtClustDB:   PRK00748
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 25590; Mature: 25590

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILFPAIDLKDGQCVRLKLGDMDQATIYNEDPAAQAKAFEDQGFEWLHVVDLNGAFAGES
CEEECEEECCCCCEEEEEECCCCCCEEECCCCCHHHHHHHCCCCEEEEEEECCCCCCCCC
VNGTAVEAILKATKNPVQLGGGIRTLAHIENWLSRGLRRVILGTVAVRDPALVMEACKAF
CCHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
PGQVAVGIDAKGGKVAVEGWAEASRLGVIELAKKFEGAGVAAIIYTDIDRDGVLAGINWD
CCCEEEEEECCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEECCCC
STLALAEAVSIPVIASGGLASMEDIRRLATPEMRKLEGAISGRALYDGRIDPAEALSVLR
HHHHHHHHHCCCEEECCCCCCHHHHHHHCCCHHHHHHHHHCCCEEECCCCCHHHHHHHHH
AAA
HCC
>Mature Secondary Structure
MILFPAIDLKDGQCVRLKLGDMDQATIYNEDPAAQAKAFEDQGFEWLHVVDLNGAFAGES
CEEECEEECCCCCEEEEEECCCCCCEEECCCCCHHHHHHHCCCCEEEEEEECCCCCCCCC
VNGTAVEAILKATKNPVQLGGGIRTLAHIENWLSRGLRRVILGTVAVRDPALVMEACKAF
CCHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
PGQVAVGIDAKGGKVAVEGWAEASRLGVIELAKKFEGAGVAAIIYTDIDRDGVLAGINWD
CCCEEEEEECCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEECCCC
STLALAEAVSIPVIASGGLASMEDIRRLATPEMRKLEGAISGRALYDGRIDPAEALSVLR
HHHHHHHHHCCCEEECCCCCCHHHHHHHCCCHHHHHHHHHCCCEEECCCCCHHHHHHHHH
AAA
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA