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The map label for this gene is gidA [H]

Identifier: 225853492

GI number: 225853492

Start: 2002959

End: 2004869

Strand: Reverse

Name: gidA [H]

Synonym: BMEA_A2122

Alternate gene names: 225853492

Gene position: 2004869-2002959 (Counterclockwise)

Preceding gene: 225853493

Following gene: 225853491

Centisome position: 94.32

GC content: 56.57

Gene sequence:

>1911_bases
ATGAGTTTGGCCGAAGCTCTTGCCTTTGATGTCATTGTAATTGGTGGTGGCCATGCGGGCTGCGAAGCCGCTTCCGCTGC
CGCGCGTGCGGGCGCGCGCACGGCGCTTGTTACGCATCGTTTTGATACGATCGGGGTCATGTCCTGTAACCCGGCGATTG
GCGGGCTGGGAAAAGGCCATCTTGTTCGTGAAATAGATGCCCTTGATGGCCTGATGGGGCGCGTGGCTGATAGAGCAGGC
ATCCAGTTTCGCTTGCTCAATCGGCGCAAGGGCCCGGCGGTGCGAGGGCCTCGTACGCAGGCTGATCGAAAGCTTTATCG
TCTGGCCATGCAGCAAATGATCACGGAACAGGAGAACCTCACCGTTGTTGAGGGTGGTGCTGCTGATCTTGTCTGCGATG
GCGAACGAATTTCCGGTGTCACTCTTGCGGATGGTCGCGTTTTGAAATGTGGTGCCGTGGTTCTCACAACAGGCACGTTT
CTTAACGGCCTTATTCATATAGGCGAAAAGAGGTTTCCAGCCGGCCGTATGGGGGAAAAGCCTGCCCTGGGTCTTTCCGA
ACGCTTGCTTTCCTTTGGCTTTACGCTTGGGCGTCTTAAAACCGGCACACCGCCGCGTCTTGATGGTCGCACAATCGACT
GGCAGAGTCTCGATATGCAATCGGCAGATGAGGAGCCTGTTCCGTTCTCGCTTATGACGGATCGCATCACCACGCCGCAG
ATCGAGTGTGGTATTACCCGCACCACGCCAGAAACGCACGATATCATCCGGGCGAATCTGCACCGCTCAGCCATGTATTC
CGGCAGTATCGAAGGGATCGGGCCGCGCTATTGTCCGTCTGTCGAGGATAAGATCGTCAAGTTCGGCGACCGTGATGGTC
ACCAGATATTCCTGGAGCCGGAAGGGCTTGATGACGATACGGTTTACCCCAACGGCATCTCGACCTCGCTGCCGGAAGAT
GTGCAGCTTGAAATTTTGAAGACCATACCCGGTCTGGAAAAGGCAGTGCTGCTCCAGCCGGGCTATGCCATTGAATATGA
CTTTATCGATCCGCGCGAGTTGAAGCGCAGCCTTGAAACGCGCAAGGTCTGCGGTCTTTTTCTGGCCGGGCAGATCAATG
GCACGACGGGATATGAGGAGGCGGGCGCTCAAGGGTTGCTGGCCGGGCTTAATGCGGCGCGCCGTGCGGCAGGGAGCGAG
CCTGTCATACTCCAGCGAACGGAAGCCTATATTGGTGTGATGGTGGATGATCTGACGTCTCGCGGCGTGAGCGAACCCTA
TCGCATGTTTACGTCGCGAGCCGAGTTCCGGCTTTCGCTGCGTGCTGATAATGCCGATCAGCGGTTGACCCCCCTTGCTG
ATGAAGTTGGAATCTTGAGCGAAGAGCGTCGTAAACGCTATTTAACGCGTGAGACTGCATTATCTCATGCGCGCATGGTG
ACGCAATCGCTGTCGATTACCCCCAATCTTGCCGGGTATTATGATCTGCGGCTCAATCAGGACGGTGTTCGCCGCTCCGC
CTATGATCTGCTTTCCTATCCCGATATCAATCTGGACCGCCTGATCGCCATCTGGCCGGAACTGGCATCGATCGATCCCG
TCACGCGGGAAGCACTTGAGATTGAAGCCCAATATGCGGTCTATATGGAGCGGCAGCAGAGTGATATTGCCGTCATGGAA
CGGGAAGAGCGGCTGCTTATACCGTCGGGGCTTGATTTTGATGCCATCTCCGGGCTTTCCAATGAATTGAAGCAAAAGCT
GAAGCAGAGGAAGCCGGAAACGATTGCGGAAGCGCAGCGCGTCGATGGGATAACCCCGGCCGCGGTGGCTTTATTGATCG
CGCAGATCAGAAAATTCGGCGGCAGGCAGAAACTGGCTGCCGAAACGCTGGAAGGCAAGGGCGCAGCATGA

Upstream 100 bases:

>100_bases
CGAATAGAAGGGCGGACATAGTTTTTCCTTCCGTTTGAAGGCCATTCATGTCAAAGAAGCTTCTTTCAGAAACGGATTCG
AATTGCGTCAGGATGAACCC

Downstream 100 bases:

>100_bases
GCGCCGATATCCGCTTTGATAGCCTGAAAACCATCGTGCCGGCTGTTTCACGTGAAACAGCCGATCGCCTGATCGCGTTC
GAGGATCTGTTCCGAAAATG

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 636; Mature: 635

Protein sequence:

>636_residues
MSLAEALAFDVIVIGGGHAGCEAASAAARAGARTALVTHRFDTIGVMSCNPAIGGLGKGHLVREIDALDGLMGRVADRAG
IQFRLLNRRKGPAVRGPRTQADRKLYRLAMQQMITEQENLTVVEGGAADLVCDGERISGVTLADGRVLKCGAVVLTTGTF
LNGLIHIGEKRFPAGRMGEKPALGLSERLLSFGFTLGRLKTGTPPRLDGRTIDWQSLDMQSADEEPVPFSLMTDRITTPQ
IECGITRTTPETHDIIRANLHRSAMYSGSIEGIGPRYCPSVEDKIVKFGDRDGHQIFLEPEGLDDDTVYPNGISTSLPED
VQLEILKTIPGLEKAVLLQPGYAIEYDFIDPRELKRSLETRKVCGLFLAGQINGTTGYEEAGAQGLLAGLNAARRAAGSE
PVILQRTEAYIGVMVDDLTSRGVSEPYRMFTSRAEFRLSLRADNADQRLTPLADEVGILSEERRKRYLTRETALSHARMV
TQSLSITPNLAGYYDLRLNQDGVRRSAYDLLSYPDINLDRLIAIWPELASIDPVTREALEIEAQYAVYMERQQSDIAVME
REERLLIPSGLDFDAISGLSNELKQKLKQRKPETIAEAQRVDGITPAAVALLIAQIRKFGGRQKLAAETLEGKGAA

Sequences:

>Translated_636_residues
MSLAEALAFDVIVIGGGHAGCEAASAAARAGARTALVTHRFDTIGVMSCNPAIGGLGKGHLVREIDALDGLMGRVADRAG
IQFRLLNRRKGPAVRGPRTQADRKLYRLAMQQMITEQENLTVVEGGAADLVCDGERISGVTLADGRVLKCGAVVLTTGTF
LNGLIHIGEKRFPAGRMGEKPALGLSERLLSFGFTLGRLKTGTPPRLDGRTIDWQSLDMQSADEEPVPFSLMTDRITTPQ
IECGITRTTPETHDIIRANLHRSAMYSGSIEGIGPRYCPSVEDKIVKFGDRDGHQIFLEPEGLDDDTVYPNGISTSLPED
VQLEILKTIPGLEKAVLLQPGYAIEYDFIDPRELKRSLETRKVCGLFLAGQINGTTGYEEAGAQGLLAGLNAARRAAGSE
PVILQRTEAYIGVMVDDLTSRGVSEPYRMFTSRAEFRLSLRADNADQRLTPLADEVGILSEERRKRYLTRETALSHARMV
TQSLSITPNLAGYYDLRLNQDGVRRSAYDLLSYPDINLDRLIAIWPELASIDPVTREALEIEAQYAVYMERQQSDIAVME
REERLLIPSGLDFDAISGLSNELKQKLKQRKPETIAEAQRVDGITPAAVALLIAQIRKFGGRQKLAAETLEGKGAA
>Mature_635_residues
SLAEALAFDVIVIGGGHAGCEAASAAARAGARTALVTHRFDTIGVMSCNPAIGGLGKGHLVREIDALDGLMGRVADRAGI
QFRLLNRRKGPAVRGPRTQADRKLYRLAMQQMITEQENLTVVEGGAADLVCDGERISGVTLADGRVLKCGAVVLTTGTFL
NGLIHIGEKRFPAGRMGEKPALGLSERLLSFGFTLGRLKTGTPPRLDGRTIDWQSLDMQSADEEPVPFSLMTDRITTPQI
ECGITRTTPETHDIIRANLHRSAMYSGSIEGIGPRYCPSVEDKIVKFGDRDGHQIFLEPEGLDDDTVYPNGISTSLPEDV
QLEILKTIPGLEKAVLLQPGYAIEYDFIDPRELKRSLETRKVCGLFLAGQINGTTGYEEAGAQGLLAGLNAARRAAGSEP
VILQRTEAYIGVMVDDLTSRGVSEPYRMFTSRAEFRLSLRADNADQRLTPLADEVGILSEERRKRYLTRETALSHARMVT
QSLSITPNLAGYYDLRLNQDGVRRSAYDLLSYPDINLDRLIAIWPELASIDPVTREALEIEAQYAVYMERQQSDIAVMER
EERLLIPSGLDFDAISGLSNELKQKLKQRKPETIAEAQRVDGITPAAVALLIAQIRKFGGRQKLAAETLEGKGAA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=644, Percent_Identity=45.4968944099379, Blast_Score=541, Evalue=1e-154,
Organism=Homo sapiens, GI19882217, Length=669, Percent_Identity=43.796711509716, Blast_Score=527, Evalue=1e-149,
Organism=Homo sapiens, GI183227703, Length=684, Percent_Identity=42.8362573099415, Blast_Score=522, Evalue=1e-148,
Organism=Escherichia coli, GI2367273, Length=618, Percent_Identity=51.7799352750809, Blast_Score=624, Evalue=1e-180,
Organism=Caenorhabditis elegans, GI17534255, Length=620, Percent_Identity=44.6774193548387, Blast_Score=517, Evalue=1e-147,
Organism=Saccharomyces cerevisiae, GI6321202, Length=627, Percent_Identity=47.6874003189793, Blast_Score=577, Evalue=1e-165,
Organism=Drosophila melanogaster, GI24658174, Length=631, Percent_Identity=48.9698890649762, Blast_Score=566, Evalue=1e-161,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 69441; Mature: 69310

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLAEALAFDVIVIGGGHAGCEAASAAARAGARTALVTHRFDTIGVMSCNPAIGGLGKGH
CCHHHHHEEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCCCCCCCH
LVREIDALDGLMGRVADRAGIQFRLLNRRKGPAVRGPRTQADRKLYRLAMQQMITEQENL
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCE
TVVEGGAADLVCDGERISGVTLADGRVLKCGAVVLTTGTFLNGLIHIGEKRFPAGRMGEK
EEEECCCCEEEECCCCCCCEEECCCCEEECCEEEEEHHHHHHHHHHHCCCCCCCCCCCCC
PALGLSERLLSFGFTLGRLKTGTPPRLDGRTIDWQSLDMQSADEEPVPFSLMTDRITTPQ
CCCCHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCC
IECGITRTTPETHDIIRANLHRSAMYSGSIEGIGPRYCPSVEDKIVKFGDRDGHQIFLEP
EEECEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEC
EGLDDDTVYPNGISTSLPEDVQLEILKTIPGLEKAVLLQPGYAIEYDFIDPRELKRSLET
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCEEEECCCCHHHHHHHHHH
RKVCGLFLAGQINGTTGYEEAGAQGLLAGLNAARRAAGSEPVILQRTEAYIGVMVDDLTS
HHHHHEEEEEEECCCCCCHHHCCHHHHHHHHHHHHHCCCCCEEEEECCHHEEHHHHHHHH
RGVSEPYRMFTSRAEFRLSLRADNADQRLTPLADEVGILSEERRKRYLTRETALSHARMV
CCCCHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHH
TQSLSITPNLAGYYDLRLNQDGVRRSAYDLLSYPDINLDRLIAIWPELASIDPVTREALE
HHHHCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHCHHHHCCCHHHHHHHH
IEAQYAVYMERQQSDIAVMEREERLLIPSGLDFDAISGLSNELKQKLKQRKPETIAEAQR
HHHHHHEEEHHCCCCHHEEECCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VDGITPAAVALLIAQIRKFGGRQKLAAETLEGKGAA
HCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
SLAEALAFDVIVIGGGHAGCEAASAAARAGARTALVTHRFDTIGVMSCNPAIGGLGKGH
CHHHHHEEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCCCCCCCH
LVREIDALDGLMGRVADRAGIQFRLLNRRKGPAVRGPRTQADRKLYRLAMQQMITEQENL
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCE
TVVEGGAADLVCDGERISGVTLADGRVLKCGAVVLTTGTFLNGLIHIGEKRFPAGRMGEK
EEEECCCCEEEECCCCCCCEEECCCCEEECCEEEEEHHHHHHHHHHHCCCCCCCCCCCCC
PALGLSERLLSFGFTLGRLKTGTPPRLDGRTIDWQSLDMQSADEEPVPFSLMTDRITTPQ
CCCCHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCC
IECGITRTTPETHDIIRANLHRSAMYSGSIEGIGPRYCPSVEDKIVKFGDRDGHQIFLEP
EEECEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEC
EGLDDDTVYPNGISTSLPEDVQLEILKTIPGLEKAVLLQPGYAIEYDFIDPRELKRSLET
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCEEEECCCCHHHHHHHHHH
RKVCGLFLAGQINGTTGYEEAGAQGLLAGLNAARRAAGSEPVILQRTEAYIGVMVDDLTS
HHHHHEEEEEEECCCCCCHHHCCHHHHHHHHHHHHHCCCCCEEEEECCHHEEHHHHHHHH
RGVSEPYRMFTSRAEFRLSLRADNADQRLTPLADEVGILSEERRKRYLTRETALSHARMV
CCCCHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHH
TQSLSITPNLAGYYDLRLNQDGVRRSAYDLLSYPDINLDRLIAIWPELASIDPVTREALE
HHHHCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHCHHHHCCCHHHHHHHH
IEAQYAVYMERQQSDIAVMEREERLLIPSGLDFDAISGLSNELKQKLKQRKPETIAEAQR
HHHHHHEEEHHCCCCHHEEECCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VDGITPAAVALLIAQIRKFGGRQKLAAETLEGKGAA
HCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA