Click here to switch to the map view.

The map label for this gene is gidB

Identifier: 225853491

GI number: 225853491

Start: 2002321

End: 2002962

Strand: Reverse

Name: gidB

Synonym: BMEA_A2121

Alternate gene names: 225853491

Gene position: 2002962-2002321 (Counterclockwise)

Preceding gene: 225853492

Following gene: 225853490

Centisome position: 94.23

GC content: 54.21

Gene sequence:

>642_bases
ATGAGCGCCGATATCCGCTTTGATAGCCTGAAAACCATCGTGCCGGCTGTTTCACGTGAAACAGCCGATCGCCTGATCGC
GTTCGAGGATCTGTTCCGAAAATGGTCGAAGGCCATCAATCTGGCTTCTCCCTCGACGCTCGCCGATCTATGGAACCGGC
ATATTCTTGATAGTGCGCAGCTTTTTCCTCTCGCAAAAGAAGCCACGCGCTGGCTGGATATCGGTTCCGGTGGCGGATTT
CCGGGGATTGTAACCGCCTGTTTTCTGGCCGAACGTTCTGGCGGCTGCATTGATCTGGTGGAAAGTGCGGGTAAAAAGGC
TGCCTTTTTACGTACGGCTGCCGGGCATCTCCATGTCCCGGCACGCGTTCACTCCGCGCGCATCGAATCCATGTGGGAAA
AAATTGAAACACCTCAGGTGGTTACGGCGCGCGCGCTTGCATCGCTGGGCGATCTTTTCACCCTTGCTGAGCCATGGTTG
AGCGACGGCGCAAAGGCCCTTTTCCAAAAAGGTCGGGATTATCAGCGCGAAATCGACGAAAGCCGTGTCGGTTGGAGTTT
CGATCTGGTAAAACATCCTAGTGCTATCGATCAGGCTTCGGTCATACTCGAAATCAGTAATCTGCGCCGTAAAACCGATT
GA

Upstream 100 bases:

>100_bases
GGATAACCCCGGCCGCGGTGGCTTTATTGATCGCGCAGATCAGAAAATTCGGCGGCAGGCAGAAACTGGCTGCCGAAACG
CTGGAAGGCAAGGGCGCAGC

Downstream 100 bases:

>100_bases
TATTTCGCAAATTGTGTTTTTCGGCAGGGCGAGCAGCAGCAAGGCGACGGTAATGAACAAGATGTCCAGGATCATAACCA
TTGCGAACCAGAAGGGTGGG

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MSADIRFDSLKTIVPAVSRETADRLIAFEDLFRKWSKAINLASPSTLADLWNRHILDSAQLFPLAKEATRWLDIGSGGGF
PGIVTACFLAERSGGCIDLVESAGKKAAFLRTAAGHLHVPARVHSARIESMWEKIETPQVVTARALASLGDLFTLAEPWL
SDGAKALFQKGRDYQREIDESRVGWSFDLVKHPSAIDQASVILEISNLRRKTD

Sequences:

>Translated_213_residues
MSADIRFDSLKTIVPAVSRETADRLIAFEDLFRKWSKAINLASPSTLADLWNRHILDSAQLFPLAKEATRWLDIGSGGGF
PGIVTACFLAERSGGCIDLVESAGKKAAFLRTAAGHLHVPARVHSARIESMWEKIETPQVVTARALASLGDLFTLAEPWL
SDGAKALFQKGRDYQREIDESRVGWSFDLVKHPSAIDQASVILEISNLRRKTD
>Mature_212_residues
SADIRFDSLKTIVPAVSRETADRLIAFEDLFRKWSKAINLASPSTLADLWNRHILDSAQLFPLAKEATRWLDIGSGGGFP
GIVTACFLAERSGGCIDLVESAGKKAAFLRTAAGHLHVPARVHSARIESMWEKIETPQVVTARALASLGDLFTLAEPWLS
DGAKALFQKGRDYQREIDESRVGWSFDLVKHPSAIDQASVILEISNLRRKTD

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=145, Percent_Identity=31.0344827586207, Blast_Score=68, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_BRUA1 (B2S959)

Other databases:

- EMBL:   CP000887
- RefSeq:   YP_001935885.1
- ProteinModelPortal:   B2S959
- SMR:   B2S959
- GeneID:   6327033
- GenomeReviews:   CP000887_GR
- KEGG:   bmc:BAbS19_I19290
- HOGENOM:   HBG686577
- OMA:   GGQGWIV
- ProtClustDB:   PRK00107
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 23473; Mature: 23342

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADIRFDSLKTIVPAVSRETADRLIAFEDLFRKWSKAINLASPSTLADLWNRHILDSAQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHH
LFPLAKEATRWLDIGSGGGFPGIVTACFLAERSGGCIDLVESAGKKAAFLRTAAGHLHVP
HHHHHHHHHHHEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHCCCEECC
ARVHSARIESMWEKIETPQVVTARALASLGDLFTLAEPWLSDGAKALFQKGRDYQREIDE
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
SRVGWSFDLVKHPSAIDQASVILEISNLRRKTD
HHCCCEEHHHHCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SADIRFDSLKTIVPAVSRETADRLIAFEDLFRKWSKAINLASPSTLADLWNRHILDSAQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHH
LFPLAKEATRWLDIGSGGGFPGIVTACFLAERSGGCIDLVESAGKKAAFLRTAAGHLHVP
HHHHHHHHHHHEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHCCCEECC
ARVHSARIESMWEKIETPQVVTARALASLGDLFTLAEPWLSDGAKALFQKGRDYQREIDE
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
SRVGWSFDLVKHPSAIDQASVILEISNLRRKTD
HHCCCEEHHHHCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA