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The map label for this gene is thiB [H]

Identifier: 225853208

GI number: 225853208

Start: 1707963

End: 1708967

Strand: Direct

Name: thiB [H]

Synonym: BMEA_A1810

Alternate gene names: 225853208

Gene position: 1707963-1708967 (Clockwise)

Preceding gene: 225853204

Following gene: 225853209

Centisome position: 80.35

GC content: 55.82

Gene sequence:

>1005_bases
ATGCGACTTTTATCCTTGCTTACCTTTTCGCTTTTTGCAGTCATCGGCCTTGCACCAGCCGCACAGGCCAAGGACAAGCT
TACTATCTATACCTATGACAGTTTCGTTTCCGAATGGGGACCCGGCCCCAAGGTGAAGGAAAACTTCGAGAAGGAATGCG
ATTGCGAGGTGAACTTCGTTGCCTCCGCCGATGGTGTCGCGCTTCTCAACCGCCTCAAGCTCGAAGGCAGCAAAACCGCC
GCCGACATTGTTTTGGGGTTAGATACCAACCTCACCACGGAAGCACGCGCATCGGGCTTTTTCGCGCCGAGCGGGATCGA
CCAGACCAATGTGAAGGTGCCCGGCAACTTCAAGGATGATATTTTCGTGCCCTATGACTATGGCTATTTTGCCGTGGTCT
ATGATTCCGAAAAATTGCCCAATCCGCCGAAAAGCCTGAAGGAACTGGTCGAAGGCGATCCAGCGCAGAAGATCGTGCTG
CAAGATCCGCGCACCGCGACACCGGGGCTTGGCATGTTGCTGTGGATGAAGTCTGTTTATGGCGACGAGGCGGGTGCTGC
ATGGCAGAAACTGCAAAAGCGCGTCCTCACCGTCACGCCCGGCTGGTCAGAAGCTTATGGCCTGTTCACCAAGGGTGAAG
CACCGATGGTGCTGTCCTACACGACCTCTCCCGCCTATCACATGGTTGTCGAAAAGACGAACCGCTATAAGGCACTGGCC
TATCCGGAAGGCAATTATCTTCAGATTGAGCTTGCCGCCCAGACCACCACCGGCGCAAAGAACCCGCTGGCGAAAAAGTT
TCTCGCCTTCATGACTGGCCCCGGCTTTCAGGATGTGATCCCCGAAACCAACTGGATGTTTCCGGCAGGCAAGACGTCCA
AGCCGCTTCCAGCCGCCTTCGATGCCCTGCCGAAGCCGGAGAAGACCCTGCTCATCCCTCCCTACGAAGTGGCGAAAAAC
CGCAGATTGTGGGTGAATGAATGGCTGGCAGCCACCAGCAGATGA

Upstream 100 bases:

>100_bases
GCCAACCCGCTGAACCTGATCCGGTTTGCACCGGCGGAGGGATTAGACGCTCGATGAACGGCGCTCGATCCTCTTGGAAT
AAACGAAAGGAAGTGCCGTG

Downstream 100 bases:

>100_bases
CAGCCACACCGGCAAGACGCACATCCCTTGCATCTCCCGCGACAAAACCTGTCGCGGGCGGTTTGGCGCTCGCCTTTCTG
GCCACACTTGCCGGGGGCGC

Product: thiamine transporter substrate binding subunit

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MRLLSLLTFSLFAVIGLAPAAQAKDKLTIYTYDSFVSEWGPGPKVKENFEKECDCEVNFVASADGVALLNRLKLEGSKTA
ADIVLGLDTNLTTEARASGFFAPSGIDQTNVKVPGNFKDDIFVPYDYGYFAVVYDSEKLPNPPKSLKELVEGDPAQKIVL
QDPRTATPGLGMLLWMKSVYGDEAGAAWQKLQKRVLTVTPGWSEAYGLFTKGEAPMVLSYTTSPAYHMVVEKTNRYKALA
YPEGNYLQIELAAQTTTGAKNPLAKKFLAFMTGPGFQDVIPETNWMFPAGKTSKPLPAAFDALPKPEKTLLIPPYEVAKN
RRLWVNEWLAATSR

Sequences:

>Translated_334_residues
MRLLSLLTFSLFAVIGLAPAAQAKDKLTIYTYDSFVSEWGPGPKVKENFEKECDCEVNFVASADGVALLNRLKLEGSKTA
ADIVLGLDTNLTTEARASGFFAPSGIDQTNVKVPGNFKDDIFVPYDYGYFAVVYDSEKLPNPPKSLKELVEGDPAQKIVL
QDPRTATPGLGMLLWMKSVYGDEAGAAWQKLQKRVLTVTPGWSEAYGLFTKGEAPMVLSYTTSPAYHMVVEKTNRYKALA
YPEGNYLQIELAAQTTTGAKNPLAKKFLAFMTGPGFQDVIPETNWMFPAGKTSKPLPAAFDALPKPEKTLLIPPYEVAKN
RRLWVNEWLAATSR
>Mature_334_residues
MRLLSLLTFSLFAVIGLAPAAQAKDKLTIYTYDSFVSEWGPGPKVKENFEKECDCEVNFVASADGVALLNRLKLEGSKTA
ADIVLGLDTNLTTEARASGFFAPSGIDQTNVKVPGNFKDDIFVPYDYGYFAVVYDSEKLPNPPKSLKELVEGDPAQKIVL
QDPRTATPGLGMLLWMKSVYGDEAGAAWQKLQKRVLTVTPGWSEAYGLFTKGEAPMVLSYTTSPAYHMVVEKTNRYKALA
YPEGNYLQIELAAQTTTGAKNPLAKKFLAFMTGPGFQDVIPETNWMFPAGKTSKPLPAAFDALPKPEKTLLIPPYEVAKN
RRLWVNEWLAATSR

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import [H]

COG id: COG4143

COG function: function code H; ABC-type thiamine transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1786255, Length=312, Percent_Identity=57.3717948717949, Blast_Score=380, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR005948
- InterPro:   IPR005967 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36737; Mature: 36737

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLSLLTFSLFAVIGLAPAAQAKDKLTIYTYDSFVSEWGPGPKVKENFEKECDCEVNFV
CHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHCCCCCCHHHHHHHCCCCEEEEE
ASADGVALLNRLKLEGSKTAADIVLGLDTNLTTEARASGFFAPSGIDQTNVKVPGNFKDD
ECCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCEEECCCCCCC
IFVPYDYGYFAVVYDSEKLPNPPKSLKELVEGDPAQKIVLQDPRTATPGLGMLLWMKSVY
EEEEECCCEEEEEECCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHH
GDEAGAAWQKLQKRVLTVTPGWSEAYGLFTKGEAPMVLSYTTSPAYHMVVEKTNRYKALA
CCCCHHHHHHHHHCEEEECCCCHHHCCEEECCCCCEEEEEECCCEEEEEEECCCCEEEEE
YPEGNYLQIELAAQTTTGAKNPLAKKFLAFMTGPGFQDVIPETNWMFPAGKTSKPLPAAF
CCCCCEEEEEEEEECCCCCCCHHHHHHHHHHCCCCHHHHCCCCCEECCCCCCCCCCCHHH
DALPKPEKTLLIPPYEVAKNRRLWVNEWLAATSR
HCCCCCCCEEEECCHHHHCCCCEEHHHHHHHCCC
>Mature Secondary Structure
MRLLSLLTFSLFAVIGLAPAAQAKDKLTIYTYDSFVSEWGPGPKVKENFEKECDCEVNFV
CHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHCCCCCCHHHHHHHCCCCEEEEE
ASADGVALLNRLKLEGSKTAADIVLGLDTNLTTEARASGFFAPSGIDQTNVKVPGNFKDD
ECCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCEEECCCCCCC
IFVPYDYGYFAVVYDSEKLPNPPKSLKELVEGDPAQKIVLQDPRTATPGLGMLLWMKSVY
EEEEECCCEEEEEECCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHH
GDEAGAAWQKLQKRVLTVTPGWSEAYGLFTKGEAPMVLSYTTSPAYHMVVEKTNRYKALA
CCCCHHHHHHHHHCEEEECCCCHHHCCEEECCCCCEEEEEECCCEEEEEEECCCCEEEEE
YPEGNYLQIELAAQTTTGAKNPLAKKFLAFMTGPGFQDVIPETNWMFPAGKTSKPLPAAF
CCCCCEEEEEEEEECCCCCCCHHHHHHHHHHCCCCHHHHCCCCCEECCCCCCCCCCCHHH
DALPKPEKTLLIPPYEVAKNRRLWVNEWLAATSR
HCCCCCCCEEEECCHHHHCCCCEEHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA