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The map label for this gene is phoA [H]

Identifier: 225852696

GI number: 225852696

Start: 1195357

End: 1196802

Strand: Direct

Name: phoA [H]

Synonym: BMEA_A1244

Alternate gene names: 225852696

Gene position: 1195357-1196802 (Clockwise)

Preceding gene: 225852695

Following gene: 225852708

Centisome position: 56.23

GC content: 58.02

Gene sequence:

>1446_bases
ATGCCCAGGAAATTCACCCGGCCACTTCTGGCAAGCGCTGCAATTATTGTCACCTTTTGCGCACCCGCAAGCACCGTGAT
GGCCCAGTCTGAAACTGCGGCTCCACTCTTCCAGCGCAGCGCAAAAGGCGACCTGTCGGCAGCAGGCGGCGCACGCCGCC
TTGATGGTGATATTACCGCAGCTTTTCGCGATCAGATCAGGAAGGGGCCTGCCAGAAACGTCATCCTGCTGATCGGAGAC
GGCATGGGCGATTCCGAGATCACGGTTGCGCGCAATTATGCAGTCGGCGCAGGCAATCATTTCAAGGGCATCGATGCCCT
TCCCGTTACCGGCCAGTACACCCATTATTCGCTCGACAAGGAAAGCGGCAAGCCAAATTACGTGACTGATTCTGCCGCCT
CCGGCACCGCCTGGGCCACTGGCGTCAAAACCTATAACGGCGCCATCGGCGTCGATATTCATGGCAAACCGCACAAAACG
CTCATTGAACTCGCCAAGGAAAAGGGCCTCGGCACCGGCAATGTCAGCACTGCCGAATTGCAGGATGCCACACCCGCCGT
GCAGGTTGCCCATGTTACCCATCGCAAGTGCTACGACCCAACCAGCACGGCGGAAAAGTGCAAGGATAATGCGCTTGAAA
ATGGCGGCCCAGGCTCGATCAGCGAACAGCTTTTGAACACCCGCCCGGACGTGACATTGGGCGGCGGCGCAAAATATTTC
GAGGACAAGGCCCGGGCTGGCACATGGAAAGGCCAGACCTTGAGCGCACAGGCCGAAGCACGCGGCTATCAACTGGTTAG
AAATGCCGACGCATTGAGCAAGGTCGAGACAGCCAATCAGGACAAACCTGTTCTCGGCCTCTTCTCACCGGGCAACATGC
CGGTCCGCTGGAAAGGGCCTCAGGCCGCCCATCACGGCAATCTCGACAGAGAGCCTGTCAAGTGCAAGGTCAATGAGAAG
CGCACTGCCGATATTCCGACACTGGCGCAAATGACCGAGAAAGCCATCGACCTGCTGAAAACCAACGAAAAAGGCTTTTT
CCTTCAGGTGGAAGGTGCCTCCATCGACAAACAGGACCATGCCGCCAATCCGTGCGGGCAGATCGGCGAAACAGTCGATC
TGGATGAAGCCGTGCAGGCCGCACTCGATTTTGCCCGCAAAGACGGCAACACGCTTGTCATCGTAACGGCCGATCACGCC
CATGCAAGCCAGATCATTGATCCCGATGCGAAGGCTCCGGGTTTGAGCCAGGCATTGAAAACCGAGGATGGCGCGATCAT
GGGCGTGACCTATGGCAATTCCGACGAAGCCGACGATCAGGGCCACACAGGCGCGCAATTGCGCATAGCCGCCTTTGGCC
CCGGCGCTGCCAATTTTACCGGCCTCACGGATCAGACGGATATGTTCTACACGATCCGCGATGCCTTGCAGTTGCAGGCG
GAATAA

Upstream 100 bases:

>100_bases
CGGCTGTCACACGCCTGCAATCTCCTTTTCGCATAAAAGCGCCATTCAGGTTTCCGGCATCACATTTTCCTGCCGGAGCG
TATGATAAGGAGAGATATGG

Downstream 100 bases:

>100_bases
TGCCAGTGCATTGGAAACGCCGTCTATAAACAGCGTTTCCATGCGGTAATCAGCGGATAAAGAACATATCCTGAAACTTG
TGCGTGCCATACACGCGGCA

Product: alkaline phosphatase

Products: NA

Alternate protein names: APase [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MPRKFTRPLLASAAIIVTFCAPASTVMAQSETAAPLFQRSAKGDLSAAGGARRLDGDITAAFRDQIRKGPARNVILLIGD
GMGDSEITVARNYAVGAGNHFKGIDALPVTGQYTHYSLDKESGKPNYVTDSAASGTAWATGVKTYNGAIGVDIHGKPHKT
LIELAKEKGLGTGNVSTAELQDATPAVQVAHVTHRKCYDPTSTAEKCKDNALENGGPGSISEQLLNTRPDVTLGGGAKYF
EDKARAGTWKGQTLSAQAEARGYQLVRNADALSKVETANQDKPVLGLFSPGNMPVRWKGPQAAHHGNLDREPVKCKVNEK
RTADIPTLAQMTEKAIDLLKTNEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQAALDFARKDGNTLVIVTADHA
HASQIIDPDAKAPGLSQALKTEDGAIMGVTYGNSDEADDQGHTGAQLRIAAFGPGAANFTGLTDQTDMFYTIRDALQLQA
E

Sequences:

>Translated_481_residues
MPRKFTRPLLASAAIIVTFCAPASTVMAQSETAAPLFQRSAKGDLSAAGGARRLDGDITAAFRDQIRKGPARNVILLIGD
GMGDSEITVARNYAVGAGNHFKGIDALPVTGQYTHYSLDKESGKPNYVTDSAASGTAWATGVKTYNGAIGVDIHGKPHKT
LIELAKEKGLGTGNVSTAELQDATPAVQVAHVTHRKCYDPTSTAEKCKDNALENGGPGSISEQLLNTRPDVTLGGGAKYF
EDKARAGTWKGQTLSAQAEARGYQLVRNADALSKVETANQDKPVLGLFSPGNMPVRWKGPQAAHHGNLDREPVKCKVNEK
RTADIPTLAQMTEKAIDLLKTNEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQAALDFARKDGNTLVIVTADHA
HASQIIDPDAKAPGLSQALKTEDGAIMGVTYGNSDEADDQGHTGAQLRIAAFGPGAANFTGLTDQTDMFYTIRDALQLQA
E
>Mature_480_residues
PRKFTRPLLASAAIIVTFCAPASTVMAQSETAAPLFQRSAKGDLSAAGGARRLDGDITAAFRDQIRKGPARNVILLIGDG
MGDSEITVARNYAVGAGNHFKGIDALPVTGQYTHYSLDKESGKPNYVTDSAASGTAWATGVKTYNGAIGVDIHGKPHKTL
IELAKEKGLGTGNVSTAELQDATPAVQVAHVTHRKCYDPTSTAEKCKDNALENGGPGSISEQLLNTRPDVTLGGGAKYFE
DKARAGTWKGQTLSAQAEARGYQLVRNADALSKVETANQDKPVLGLFSPGNMPVRWKGPQAAHHGNLDREPVKCKVNEKR
TADIPTLAQMTEKAIDLLKTNEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQAALDFARKDGNTLVIVTADHAH
ASQIIDPDAKAPGLSQALKTEDGAIMGVTYGNSDEADDQGHTGAQLRIAAFGPGAANFTGLTDQTDMFYTIRDALQLQAE

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI116734717, Length=370, Percent_Identity=30, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI157266296, Length=379, Percent_Identity=30.6068601583113, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI94721246, Length=374, Percent_Identity=30.2139037433155, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI294660770, Length=365, Percent_Identity=29.3150684931507, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI157266292, Length=396, Percent_Identity=28.5353535353535, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI294660772, Length=313, Percent_Identity=29.7124600638978, Blast_Score=107, Evalue=3e-23,
Organism=Escherichia coli, GI48994877, Length=444, Percent_Identity=71.6216216216216, Blast_Score=660, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320689, Length=350, Percent_Identity=31.7142857142857, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI21355151, Length=405, Percent_Identity=30.3703703703704, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI21355981, Length=355, Percent_Identity=29.0140845070423, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19921912, Length=459, Percent_Identity=27.4509803921569, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24585213, Length=417, Percent_Identity=29.0167865707434, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21355149, Length=471, Percent_Identity=26.5392781316348, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI85725200, Length=389, Percent_Identity=29.0488431876607, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI85815833, Length=389, Percent_Identity=29.0488431876607, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24651554, Length=399, Percent_Identity=30.5764411027569, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI18859923, Length=344, Percent_Identity=27.906976744186, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21358067, Length=473, Percent_Identity=25.5813953488372, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24657827, Length=496, Percent_Identity=27.0161290322581, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 50846; Mature: 50715

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRKFTRPLLASAAIIVTFCAPASTVMAQSETAAPLFQRSAKGDLSAAGGARRLDGDITA
CCCCHHHHHHHHHEEEEEECCCCHHHHHCCCHHHHHHHHCCCCCCCCCCCCEECCCCHHH
AFRDQIRKGPARNVILLIGDGMGDSEITVARNYAVGAGNHFKGIDALPVTGQYTHYSLDK
HHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCEEEECCC
ESGKPNYVTDSAASGTAWATGVKTYNGAIGVDIHGKPHKTLIELAKEKGLGTGNVSTAEL
CCCCCCEECCCCCCCCEEECCCEEECCEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHH
QDATPAVQVAHVTHRKCYDPTSTAEKCKDNALENGGPGSISEQLLNTRPDVTLGGGAKYF
CCCCCCEEEEHHHHHHCCCCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEECCCCHHH
EDKARAGTWKGQTLSAQAEARGYQLVRNADALSKVETANQDKPVLGLFSPGNMPVRWKGP
HHHHHCCCCCCCEEECHHHHCCHHHHHCHHHHHHHHHCCCCCCEEEEECCCCCCEEECCC
QAAHHGNLDREPVKCKVNEKRTADIPTLAQMTEKAIDLLKTNEKGFFLQVEGASIDKQDH
CCHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
AANPCGQIGETVDLDEAVQAALDFARKDGNTLVIVTADHAHASQIIDPDAKAPGLSQALK
CCCCHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHCCCCCCCCCCHHHHHH
TEDGAIMGVTYGNSDEADDQGHTGAQLRIAAFGPGAANFTGLTDQTDMFYTIRDALQLQA
CCCCEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEHHHHCCC
E
C
>Mature Secondary Structure 
PRKFTRPLLASAAIIVTFCAPASTVMAQSETAAPLFQRSAKGDLSAAGGARRLDGDITA
CCCHHHHHHHHHEEEEEECCCCHHHHHCCCHHHHHHHHCCCCCCCCCCCCEECCCCHHH
AFRDQIRKGPARNVILLIGDGMGDSEITVARNYAVGAGNHFKGIDALPVTGQYTHYSLDK
HHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCEEEECCC
ESGKPNYVTDSAASGTAWATGVKTYNGAIGVDIHGKPHKTLIELAKEKGLGTGNVSTAEL
CCCCCCEECCCCCCCCEEECCCEEECCEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHH
QDATPAVQVAHVTHRKCYDPTSTAEKCKDNALENGGPGSISEQLLNTRPDVTLGGGAKYF
CCCCCCEEEEHHHHHHCCCCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEECCCCHHH
EDKARAGTWKGQTLSAQAEARGYQLVRNADALSKVETANQDKPVLGLFSPGNMPVRWKGP
HHHHHCCCCCCCEEECHHHHCCHHHHHCHHHHHHHHHCCCCCCEEEEECCCCCCEEECCC
QAAHHGNLDREPVKCKVNEKRTADIPTLAQMTEKAIDLLKTNEKGFFLQVEGASIDKQDH
CCHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
AANPCGQIGETVDLDEAVQAALDFARKDGNTLVIVTADHAHASQIIDPDAKAPGLSQALK
CCCCHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHCCCCCCCCCCHHHHHH
TEDGAIMGVTYGNSDEADDQGHTGAQLRIAAFGPGAANFTGLTDQTDMFYTIRDALQLQA
CCCCEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEHHHHCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2129542 [H]