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The map label for this gene is ispF [H]

Identifier: 225852621

GI number: 225852621

Start: 1117354

End: 1118709

Strand: Direct

Name: ispF [H]

Synonym: BMEA_A1165

Alternate gene names: 225852621

Gene position: 1117354-1118709 (Clockwise)

Preceding gene: 225852606

Following gene: 225852622

Centisome position: 52.56

GC content: 57.82

Gene sequence:

>1356_bases
TTGATATGCATTATGAAATCCGCATACAGTTTAATCACCCCGCTTTCCGAACGCAAGGAAAAGCAGCCTGAACGCACGAT
AAATCAATTGCCGTCTGGCATTTGTCGTCGCAAGGCCTTATTCGGCTTGCAGGCACAATCTTTGGCAGAGGTTACAGTCA
TTTCAAAGGCAGCAGCAGTCATCGTGGCGGCCGGTCGCGGCGAGCGGGCGGGGCAAAGCGCCGAGGGGCCGAAGCAATAT
CGGCTCATTGGCGCAGAGGCCGTGCTTGCCCGCACTTTGCGCGCTTTTACCGATTGCCCGCTCATCGGCACAATCGCCGT
CGTCATTCATCCCGACGATCATGCGCTTTATCGGCGCGCTGTGCCGGAGAAGCATGAGAACGTCATCCTCGTTACCGGTG
GCCCCACCCGACAGGAATCGACCCGCCTCGGTCTTCTGGCCTTGAAGGACGAAGCGCCGCAATATGTGTTGATCCATGAT
GGCGTGCGTCCTTTCATCGGTCAGGATTTGCTGGAACGCATCATTGCAAACCTCACCCCTGATAATGGTGTGCTTCCCGC
CCTCGCCGTCTCCGACACGTTGAAACGGGCGGCGGCAGACGGCATGGTAGAGACCACCATTTCCCGTACCGGCTTGTTCG
CGGCGCAGACGCCGCAAGCTTTTCCCTATGCGCCGATCTTGGACGCACATGAGAAAGCCTTCGCAATCAACCGCACCGAC
TTTACCGACGATGCCGCCATTGCCGAATGGCAGGAAATTGCTGTTCGCATTATCGAAGGTTCGGCGGATAATACAAAGCT
CACCTGGGCAAAGGATATTGAAATGGCAGACAAGCGCCTGCGGCAGGACCATGCCGTATTTCCCGACATTCGCACGGGCA
ATGGCTATGATGTTCATTCTTTCGAGCCGGGTGATCATGTCACGCTTTGCGGCGTGAAAATCCCCCATGAGGCAAAGCTC
AACGGTCACTCCGATGCCGATGTGGCCCTTCATGCGCTGACCGACGCCCTGCTCGCCACGCGCGGTGCGGGTGATATTGG
CACACATTTTCCGCCATCCGATCCGCAATGGAAAGGAGCTGCCTCACGCATCTTCATCGAACATGCGGCCAAAATCGTCC
GCGAGGCGGGAGGCCGGATCGCCAATGTGGACGTGACACTCATCAGTGAAGCGCCGAAGATCGGCCCCCATCGCGCCGCC
ATGACACAAGCACTCTGCGACATGCTGGGCATCGCCGCCGACCGGGTTTCCATCAAGGCTACGACGAACGAAAAGCTTGG
CTTTGTCGGCCGTCGCGAAGGCATTGCAGCCATCGCAACCGCAACCGTCATCTACCCCGGAGAAGTACCGGAATGA

Upstream 100 bases:

>100_bases
TCGGAAACACCGGACATCGGCGCAAGAAAAACGCGGTTCTTGAGCATCACACCGCGAATATTCAAAGGTTGATCGAGGGA
AGTCACCAATAAGCGCCTAT

Downstream 100 bases:

>100_bases
GCCACATCATCGCCGGACAGAAAGCCATCGCCGTGCTTGATGCCTGCCGCAAGGCAGGCATCATGATCGCAACGGCGGAA
TCCTGCACCGGCGGACTGAT

Product: bifunctional 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase/2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase protein

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MICIMKSAYSLITPLSERKEKQPERTINQLPSGICRRKALFGLQAQSLAEVTVISKAAAVIVAAGRGERAGQSAEGPKQY
RLIGAEAVLARTLRAFTDCPLIGTIAVVIHPDDHALYRRAVPEKHENVILVTGGPTRQESTRLGLLALKDEAPQYVLIHD
GVRPFIGQDLLERIIANLTPDNGVLPALAVSDTLKRAAADGMVETTISRTGLFAAQTPQAFPYAPILDAHEKAFAINRTD
FTDDAAIAEWQEIAVRIIEGSADNTKLTWAKDIEMADKRLRQDHAVFPDIRTGNGYDVHSFEPGDHVTLCGVKIPHEAKL
NGHSDADVALHALTDALLATRGAGDIGTHFPPSDPQWKGAASRIFIEHAAKIVREAGGRIANVDVTLISEAPKIGPHRAA
MTQALCDMLGIAADRVSIKATTNEKLGFVGRREGIAAIATATVIYPGEVPE

Sequences:

>Translated_451_residues
MICIMKSAYSLITPLSERKEKQPERTINQLPSGICRRKALFGLQAQSLAEVTVISKAAAVIVAAGRGERAGQSAEGPKQY
RLIGAEAVLARTLRAFTDCPLIGTIAVVIHPDDHALYRRAVPEKHENVILVTGGPTRQESTRLGLLALKDEAPQYVLIHD
GVRPFIGQDLLERIIANLTPDNGVLPALAVSDTLKRAAADGMVETTISRTGLFAAQTPQAFPYAPILDAHEKAFAINRTD
FTDDAAIAEWQEIAVRIIEGSADNTKLTWAKDIEMADKRLRQDHAVFPDIRTGNGYDVHSFEPGDHVTLCGVKIPHEAKL
NGHSDADVALHALTDALLATRGAGDIGTHFPPSDPQWKGAASRIFIEHAAKIVREAGGRIANVDVTLISEAPKIGPHRAA
MTQALCDMLGIAADRVSIKATTNEKLGFVGRREGIAAIATATVIYPGEVPE
>Mature_451_residues
MICIMKSAYSLITPLSERKEKQPERTINQLPSGICRRKALFGLQAQSLAEVTVISKAAAVIVAAGRGERAGQSAEGPKQY
RLIGAEAVLARTLRAFTDCPLIGTIAVVIHPDDHALYRRAVPEKHENVILVTGGPTRQESTRLGLLALKDEAPQYVLIHD
GVRPFIGQDLLERIIANLTPDNGVLPALAVSDTLKRAAADGMVETTISRTGLFAAQTPQAFPYAPILDAHEKAFAINRTD
FTDDAAIAEWQEIAVRIIEGSADNTKLTWAKDIEMADKRLRQDHAVFPDIRTGNGYDVHSFEPGDHVTLCGVKIPHEAKL
NGHSDADVALHALTDALLATRGAGDIGTHFPPSDPQWKGAASRIFIEHAAKIVREAGGRIANVDVTLISEAPKIGPHRAA
MTQALCDMLGIAADRVSIKATTNEKLGFVGRREGIAAIATATVIYPGEVPE

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=274, Percent_Identity=27.007299270073, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1789103, Length=155, Percent_Identity=49.6774193548387, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1789104, Length=225, Percent_Identity=34.6666666666667, Blast_Score=108, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF01128 IspD; PF02542 YgbB [H]

EC number: =2.7.7.60; =4.6.1.12 [H]

Molecular weight: Translated: 48493; Mature: 48493

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS01295 ISPD ; PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MICIMKSAYSLITPLSERKEKQPERTINQLPSGICRRKALFGLQAQSLAEVTVISKAAAV
CEEEEECHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCEEE
IVAAGRGERAGQSAEGPKQYRLIGAEAVLARTLRAFTDCPLIGTIAVVIHPDDHALYRRA
EEECCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHC
VPEKHENVILVTGGPTRQESTRLGLLALKDEAPQYVLIHDGVRPFIGQDLLERIIANLTP
CCCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHCCC
DNGVLPALAVSDTLKRAAADGMVETTISRTGLFAAQTPQAFPYAPILDAHEKAFAINRTD
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCEEEECCCC
FTDDAAIAEWQEIAVRIIEGSADNTKLTWAKDIEMADKRLRQDHAVFPDIRTGNGYDVHS
CCCCHHHHHHHHHHEEEEECCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCEECCC
FEPGDHVTLCGVKIPHEAKLNGHSDADVALHALTDALLATRGAGDIGTHFPPSDPQWKGA
CCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCH
ASRIFIEHAAKIVREAGGRIANVDVTLISEAPKIGPHRAAMTQALCDMLGIAADRVSIKA
HHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEE
TTNEKLGFVGRREGIAAIATATVIYPGEVPE
ECCCCCCCCCCCCCCEEEEEEEEEECCCCCC
>Mature Secondary Structure
MICIMKSAYSLITPLSERKEKQPERTINQLPSGICRRKALFGLQAQSLAEVTVISKAAAV
CEEEEECHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCEEE
IVAAGRGERAGQSAEGPKQYRLIGAEAVLARTLRAFTDCPLIGTIAVVIHPDDHALYRRA
EEECCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHC
VPEKHENVILVTGGPTRQESTRLGLLALKDEAPQYVLIHDGVRPFIGQDLLERIIANLTP
CCCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHCCC
DNGVLPALAVSDTLKRAAADGMVETTISRTGLFAAQTPQAFPYAPILDAHEKAFAINRTD
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCEEEECCCC
FTDDAAIAEWQEIAVRIIEGSADNTKLTWAKDIEMADKRLRQDHAVFPDIRTGNGYDVHS
CCCCHHHHHHHHHHEEEEECCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCEECCC
FEPGDHVTLCGVKIPHEAKLNGHSDADVALHALTDALLATRGAGDIGTHFPPSDPQWKGA
CCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCH
ASRIFIEHAAKIVREAGGRIANVDVTLISEAPKIGPHRAAMTQALCDMLGIAADRVSIKA
HHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEE
TTNEKLGFVGRREGIAAIATATVIYPGEVPE
ECCCCCCCCCCCCCCEEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA