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The map label for this gene is ycjG [H]

Identifier: 225852523

GI number: 225852523

Start: 1002316

End: 1003296

Strand: Reverse

Name: ycjG [H]

Synonym: BMEA_A1059

Alternate gene names: 225852523

Gene position: 1003296-1002316 (Counterclockwise)

Preceding gene: 225852528

Following gene: 225852522

Centisome position: 47.2

GC content: 57.39

Gene sequence:

>981_bases
ATGCACAATACATTGAAAATCATTCATGAACGCTATCCTATCGCGGGGAAATTCACAATCTCACGCGGCTCTAAAACAGA
AGCCGCTGTTGTGGTCTGCGTAATTGGTGACGGCATTCACGAAGGACGCGGCGAATGTGTGCCCTATGCGCGTTATGGCG
AAACCATCGAAAGCGTCAGCGGCCAGATCGAAGCAGTGCGCACGGCCATTGAAAAGGGAGCCTTACGGCATGAGCTTCAA
AAGCTTATGCCGGCGGGCGCTGCGCGCAATGCCGTGGATTGCGCGCTGTGGGATCTGGAAGCCAAACAATCCGGCAAAAG
GGTTGCCGATATGCTGGGCGGCAGTGCCGCCGCTCTTGAAACCGCAATCACTGTTTCGCTTGGAACGCCTGAGGAAATGG
CGCAATCAACTGCGAAGGTTGCGCATTATCCGCTTATAAAAGTGAAGATGGGCGGCGAAAACGACATTGAACGTATCCAT
GCCGTGGCAAAAGCGGCGCCCGGCAGCCGCATCATCATCGATGCCAATGAAGGTTGGACCGACGCCAATATCGAGGAAAA
CATGAAGGCGGCGGCAGAAGCCGGCGTCATCCTGATTGAGCAGCCTTTGCCGGCAGGCAAGGACGAAATCCTCAGCCGCA
TCGAACGGCCGGTCATCATCTGTGCGGATGAAAGTGCCCATACGTCCGAAGGGCTGGAAGAGCTTGCCCGGCGCTATGAT
GCCGTCAATATCAAGCTCGACAAGACCGGCGGCTTGACCGAAGGGCTCATCATGCGCGACAAAACCATCGATCTGGGCCT
GAAAATCATGGTAGGCTGCATGGTGGGCACGTCGCTCGGCATGGCCCCTGCCGTCCTTCTGGCGCAGAAGGCCACTGTTG
TCGACCTGGATGGCCCTCTCCTGCTTGCCCGTGACCGGGAGCCCGGCCTTCATTATGACGGCGCACTTGTCTATCCGCCG
GAGCCAGGTCTGTGGGGCTGA

Upstream 100 bases:

>100_bases
TCGTCGTAGCTCTTGCTTTGTCGGCAGCGTCGGTCAACATGGCCTACCTCATATACGAGTCGTTGAACGACCGAAATACT
GCGTCCCGAAGGGCACTTCT

Downstream 100 bases:

>100_bases
TCGGTTCCAGTGCCCATCACACCTCTCACCATATTTCATGTGACAGATTACACTCACGATCTGCCATTTTGGAAAAACTC
ATTATAGAAAATGAGCCTAC

Product: mandelate racemase/muconate lactonizing protein

Products: cis,cis-hexadienedioate

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MHNTLKIIHERYPIAGKFTISRGSKTEAAVVVCVIGDGIHEGRGECVPYARYGETIESVSGQIEAVRTAIEKGALRHELQ
KLMPAGAARNAVDCALWDLEAKQSGKRVADMLGGSAAALETAITVSLGTPEEMAQSTAKVAHYPLIKVKMGGENDIERIH
AVAKAAPGSRIIIDANEGWTDANIEENMKAAAEAGVILIEQPLPAGKDEILSRIERPVIICADESAHTSEGLEELARRYD
AVNIKLDKTGGLTEGLIMRDKTIDLGLKIMVGCMVGTSLGMAPAVLLAQKATVVDLDGPLLLARDREPGLHYDGALVYPP
EPGLWG

Sequences:

>Translated_326_residues
MHNTLKIIHERYPIAGKFTISRGSKTEAAVVVCVIGDGIHEGRGECVPYARYGETIESVSGQIEAVRTAIEKGALRHELQ
KLMPAGAARNAVDCALWDLEAKQSGKRVADMLGGSAAALETAITVSLGTPEEMAQSTAKVAHYPLIKVKMGGENDIERIH
AVAKAAPGSRIIIDANEGWTDANIEENMKAAAEAGVILIEQPLPAGKDEILSRIERPVIICADESAHTSEGLEELARRYD
AVNIKLDKTGGLTEGLIMRDKTIDLGLKIMVGCMVGTSLGMAPAVLLAQKATVVDLDGPLLLARDREPGLHYDGALVYPP
EPGLWG
>Mature_326_residues
MHNTLKIIHERYPIAGKFTISRGSKTEAAVVVCVIGDGIHEGRGECVPYARYGETIESVSGQIEAVRTAIEKGALRHELQ
KLMPAGAARNAVDCALWDLEAKQSGKRVADMLGGSAAALETAITVSLGTPEEMAQSTAKVAHYPLIKVKMGGENDIERIH
AVAKAAPGSRIIIDANEGWTDANIEENMKAAAEAGVILIEQPLPAGKDEILSRIERPVIICADESAHTSEGLEELARRYD
AVNIKLDKTGGLTEGLIMRDKTIDLGLKIMVGCMVGTSLGMAPAVLLAQKATVVDLDGPLLLARDREPGLHYDGALVYPP
EPGLWG

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has a role in the recycling of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of all the L-Ala-X dipeptides, excep

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=312, Percent_Identity=44.8717948717949, Blast_Score=255, Evalue=3e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: 5.5.1.1

Molecular weight: Translated: 34727; Mature: 34727

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNTLKIIHERYPIAGKFTISRGSKTEAAVVVCVIGDGIHEGRGECVPYARYGETIESVS
CCCHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHC
GQIEAVRTAIEKGALRHELQKLMPAGAARNAVDCALWDLEAKQSGKRVADMLGGSAAALE
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHCHHHHHHHHCCCHHHEE
TAITVSLGTPEEMAQSTAKVAHYPLIKVKMGGENDIERIHAVAKAAPGSRIIIDANEGWT
EEEEEEECCHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCC
DANIEENMKAAAEAGVILIEQPLPAGKDEILSRIERPVIICADESAHTSEGLEELARRYD
CCCHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCC
AVNIKLDKTGGLTEGLIMRDKTIDLGLKIMVGCMVGTSLGMAPAVLLAQKATVVDLDGPL
EEEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCEEEECCCCE
LLARDREPGLHYDGALVYPPEPGLWG
EEEECCCCCCCCCCEEEECCCCCCCC
>Mature Secondary Structure
MHNTLKIIHERYPIAGKFTISRGSKTEAAVVVCVIGDGIHEGRGECVPYARYGETIESVS
CCCHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHC
GQIEAVRTAIEKGALRHELQKLMPAGAARNAVDCALWDLEAKQSGKRVADMLGGSAAALE
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHCHHHHHHHHCCCHHHEE
TAITVSLGTPEEMAQSTAKVAHYPLIKVKMGGENDIERIHAVAKAAPGSRIIIDANEGWT
EEEEEEECCHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCC
DANIEENMKAAAEAGVILIEQPLPAGKDEILSRIERPVIICADESAHTSEGLEELARRYD
CCCHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCC
AVNIKLDKTGGLTEGLIMRDKTIDLGLKIMVGCMVGTSLGMAPAVLLAQKATVVDLDGPL
EEEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCEEEECCCCE
LLARDREPGLHYDGALVYPPEPGLWG
EEEECCCCCCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-dihydro-5-oxofuran-2-acetate

Specific reaction: 2,5-dihydro-5-oxofuran-2-acetate = cis,cis-hexadienedioate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7499381; 9097039; 9278503 [H]