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The map label for this gene is dme [H]

Identifier: 225852522

GI number: 225852522

Start: 999774

End: 1002098

Strand: Reverse

Name: dme [H]

Synonym: BMEA_A1058

Alternate gene names: 225852522

Gene position: 1002098-999774 (Counterclockwise)

Preceding gene: 225852523

Following gene: 225852513

Centisome position: 47.14

GC content: 59.66

Gene sequence:

>2325_bases
ATGACCAAGAAACCTTCCTCCCCTTCGACGCGCTCTGACTTCGACGAAGCGGCGCTGTTCTTCCATCGCTATCCGAAACC
GGGAAAGCTGGAAATTCAGGCGACGAAACCGCTCGGCAATCAGCGCGACCTGGCGCTTGCCTATTCGCCGGGCGTTGCAG
CGCCCTGCCTCGCCATCCATGACGATCCCGCGACCGCAGCCGAATATACGGCACGCGGCAATCTGGTTGCCGTTGTGTCC
AATGGCACGGCCGTTCTGGGCCTCGGCAATATCGGCGCACTTGCCTCCAAGCCGGTGATGGAAGGCAAGGCAGTTCTGTT
CAAGAAATTTGCCGATATCGATGTTTTCGACATTGAAATCGATGCGCATGAGATCAACCGGATTGTCGATGTGGTGTCCG
CTCTGGAACCCACTTTCGGCGGCATCAATCTGGAAGACATCAAGGCGCCGGAGTGCTTCGAGGTTGAGGAACAGCTCCGC
GAGCGGATGAATATTCCGGTCTTCCATGATGATCAGCATGGAACCGCGATCATCGTCGCCTCTGCCGTGCTCAACGGTCT
GGAGCTTGCCGGCAAGAAGATCGAGGAGGCGAAGATCGTCACCTCCGGTGCGGGTGCTGCCGCCCTTGCCTGCCTCAATC
TGCTCGTCAATCTTGGCGCGAAGCGTGAGAATATCTGGGTCTGCGATATCGAGGGCGTGGTCTATGACGGCCGCAACACG
CTCATGGACCGCTGGAAGGAAGTCTATGCGCAAAAGACCGACGCGCGCACGCTTGCCGACGTCATCGGCGGGGCAGACGT
ATTCCTCGGGCTTTCGGCTGCGGGCGTTCTCAAGCCCGAACTTTTGAAGCAGATGGCCGAAAAGCCGCTGATTATGGCGC
TTGCCAATCCAAAGCCGGAAATCATGCCGGAGGAAGCGCGCGCCGCGCGCGCGGATGCCATGATCTGCACGGGCCGTTCG
GATTTTCCCAATCAGGTCAATAATGTTCTCTGCTTCCCCTATATTTTCCGCGGCGCGCTTGACGTCGGCGCAACGGTCAT
CAACGAGGAAATGAAGCTTGCCGCAGTGCGCGCCATCGCTTCGCTTGCCCGCGAAGAGCCTTCAGATGTCGCCGCCCGCG
CCTATTCCGGCGATACGCCGACCTTCGGCCCGGATTATATCATTCCCTCGCCGTTCGATCAGCGCCTCATCCTGCGCATT
GCGCCTGCCGTCGCCAAGGCGGCGATGGAAACCGGCGTGGCAACGCGCCCGATCGAGGATATGGAAGCCTATCTCGATCG
GCTCAACCGGTTTGTCTTCCGCTCCGGCCTTATTATGAAGCCGGTCTTCGCGGCTGCCCGCACGCAAGGTGCGCCCATGC
GCGTGATCTATGCCGATGGGGAAGATGAGCGCGTGCTGCGCGCAGCACAGGTGGTGATCGAGGAGCGCATCGCTGCACCG
ATACTCGTCGGGCGACCGCAGGTCGTGGAGACTCGCCTGCGCCGTTACGGCCTCAAAATCCGCCCCGGCACCGATTTCGA
GCTTATCAACCCCGAAGACGATCCGCGCTATCGCGACTATGTCGATCTTTATCTGTCCTATACGGGCCGTCAGGGCGTTA
CGCCGGAAGCTGCGCGCACAATCGTCCGCACCAACTCGACCGCCATTGCAGCCCTTGCCGTGATGCGCGACGAGGCCGAC
GCGATGATCTGCGGCCTTGAAGGCCGTTTTGAACGCCATCTGCGCGTCGTTCGCCAGATTATCGGCAAGGTGCCCGGCAT
CCGGGATTATTCAGCCCTGAGCCTGCTCATCTCGCAGCGCGGCGCGTTGTTCCTCACCGATACCTATGTCAATGCCGAAC
CCGATGCGAACGAAATTGCCGAAATGGCAGTTCTTGCCGCCCGCGAGATCAGCCGCTTCGGTATCGAACCAAAGGCCGCG
CTCGTATCGCATTCAAATTTCGGCTCAAAGGAATCGGCAAGCGCGGAAAAAATGCGCCAGGCTGCGGCCATTCTCGCGGA
AAAGGCTCCAGATCTTGAATCCGACGGTGAAATGCATGGCGATGCCGCTCTTTCGCAGACGCTTCGTGACCGGGTCTTCC
CAAGCTCGCGCCTCAAGGGCGAAGCCAATCTTCTGGTATTCCCGAACCTCGATGCAGCAAACATCACACTTAATGTGGTC
AAGGCCGTCACGGATGCGCTGCATGTTGGCCCAATTCTTCTTGGAGCAACCCGTCCGGCCCATATTCTGACGCCTTCCGT
CACATCGCGCGGTGTCGTCAACATGACGGCGCTGGCCGTCGTCGAAGCCTTGCAAAAGAACGCCAGCAATCGTCGGCAGA
AGTAA

Upstream 100 bases:

>100_bases
GGCTAAAAACACAATAATCGGCCCGGCGTACAGCCAATACGCCTTTTACGCGCTCTATTTTTCCCGAATTAAGCGCGAAA
GAATTGGAAAGCGATGCAAG

Downstream 100 bases:

>100_bases
TTTTAGATTATGCTTTAAAAGCATATTGAAGTATTAGACGAATAAAACGCCGGGTATGACAATGGTTATACTCGGCGTTT
ACTTTTCATTAAAGCCATAC

Product: malic enzyme

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 774; Mature: 773

Protein sequence:

>774_residues
MTKKPSSPSTRSDFDEAALFFHRYPKPGKLEIQATKPLGNQRDLALAYSPGVAAPCLAIHDDPATAAEYTARGNLVAVVS
NGTAVLGLGNIGALASKPVMEGKAVLFKKFADIDVFDIEIDAHEINRIVDVVSALEPTFGGINLEDIKAPECFEVEEQLR
ERMNIPVFHDDQHGTAIIVASAVLNGLELAGKKIEEAKIVTSGAGAAALACLNLLVNLGAKRENIWVCDIEGVVYDGRNT
LMDRWKEVYAQKTDARTLADVIGGADVFLGLSAAGVLKPELLKQMAEKPLIMALANPKPEIMPEEARAARADAMICTGRS
DFPNQVNNVLCFPYIFRGALDVGATVINEEMKLAAVRAIASLAREEPSDVAARAYSGDTPTFGPDYIIPSPFDQRLILRI
APAVAKAAMETGVATRPIEDMEAYLDRLNRFVFRSGLIMKPVFAAARTQGAPMRVIYADGEDERVLRAAQVVIEERIAAP
ILVGRPQVVETRLRRYGLKIRPGTDFELINPEDDPRYRDYVDLYLSYTGRQGVTPEAARTIVRTNSTAIAALAVMRDEAD
AMICGLEGRFERHLRVVRQIIGKVPGIRDYSALSLLISQRGALFLTDTYVNAEPDANEIAEMAVLAAREISRFGIEPKAA
LVSHSNFGSKESASAEKMRQAAAILAEKAPDLESDGEMHGDAALSQTLRDRVFPSSRLKGEANLLVFPNLDAANITLNVV
KAVTDALHVGPILLGATRPAHILTPSVTSRGVVNMTALAVVEALQKNASNRRQK

Sequences:

>Translated_774_residues
MTKKPSSPSTRSDFDEAALFFHRYPKPGKLEIQATKPLGNQRDLALAYSPGVAAPCLAIHDDPATAAEYTARGNLVAVVS
NGTAVLGLGNIGALASKPVMEGKAVLFKKFADIDVFDIEIDAHEINRIVDVVSALEPTFGGINLEDIKAPECFEVEEQLR
ERMNIPVFHDDQHGTAIIVASAVLNGLELAGKKIEEAKIVTSGAGAAALACLNLLVNLGAKRENIWVCDIEGVVYDGRNT
LMDRWKEVYAQKTDARTLADVIGGADVFLGLSAAGVLKPELLKQMAEKPLIMALANPKPEIMPEEARAARADAMICTGRS
DFPNQVNNVLCFPYIFRGALDVGATVINEEMKLAAVRAIASLAREEPSDVAARAYSGDTPTFGPDYIIPSPFDQRLILRI
APAVAKAAMETGVATRPIEDMEAYLDRLNRFVFRSGLIMKPVFAAARTQGAPMRVIYADGEDERVLRAAQVVIEERIAAP
ILVGRPQVVETRLRRYGLKIRPGTDFELINPEDDPRYRDYVDLYLSYTGRQGVTPEAARTIVRTNSTAIAALAVMRDEAD
AMICGLEGRFERHLRVVRQIIGKVPGIRDYSALSLLISQRGALFLTDTYVNAEPDANEIAEMAVLAAREISRFGIEPKAA
LVSHSNFGSKESASAEKMRQAAAILAEKAPDLESDGEMHGDAALSQTLRDRVFPSSRLKGEANLLVFPNLDAANITLNVV
KAVTDALHVGPILLGATRPAHILTPSVTSRGVVNMTALAVVEALQKNASNRRQK
>Mature_773_residues
TKKPSSPSTRSDFDEAALFFHRYPKPGKLEIQATKPLGNQRDLALAYSPGVAAPCLAIHDDPATAAEYTARGNLVAVVSN
GTAVLGLGNIGALASKPVMEGKAVLFKKFADIDVFDIEIDAHEINRIVDVVSALEPTFGGINLEDIKAPECFEVEEQLRE
RMNIPVFHDDQHGTAIIVASAVLNGLELAGKKIEEAKIVTSGAGAAALACLNLLVNLGAKRENIWVCDIEGVVYDGRNTL
MDRWKEVYAQKTDARTLADVIGGADVFLGLSAAGVLKPELLKQMAEKPLIMALANPKPEIMPEEARAARADAMICTGRSD
FPNQVNNVLCFPYIFRGALDVGATVINEEMKLAAVRAIASLAREEPSDVAARAYSGDTPTFGPDYIIPSPFDQRLILRIA
PAVAKAAMETGVATRPIEDMEAYLDRLNRFVFRSGLIMKPVFAAARTQGAPMRVIYADGEDERVLRAAQVVIEERIAAPI
LVGRPQVVETRLRRYGLKIRPGTDFELINPEDDPRYRDYVDLYLSYTGRQGVTPEAARTIVRTNSTAIAALAVMRDEADA
MICGLEGRFERHLRVVRQIIGKVPGIRDYSALSLLISQRGALFLTDTYVNAEPDANEIAEMAVLAAREISRFGIEPKAAL
VSHSNFGSKESASAEKMRQAAAILAEKAPDLESDGEMHGDAALSQTLRDRVFPSSRLKGEANLLVFPNLDAANITLNVVK
AVTDALHVGPILLGATRPAHILTPSVTSRGVVNMTALAVVEALQKNASNRRQK

Specific function: Required for symbiotic nitrogen fixation. Plays a key role in the conversion of malate to acetyl-CoA for efficient tricarboxylic acid cycle function in nitrogen-fixating bacteria [H]

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=404, Percent_Identity=24.7524752475248, Blast_Score=83, Evalue=1e-15,
Organism=Homo sapiens, GI62420880, Length=404, Percent_Identity=24.7524752475248, Blast_Score=83, Evalue=1e-15,
Organism=Homo sapiens, GI239049447, Length=404, Percent_Identity=24.7524752475248, Blast_Score=83, Evalue=1e-15,
Organism=Homo sapiens, GI4505143, Length=426, Percent_Identity=22.7699530516432, Blast_Score=78, Evalue=4e-14,
Organism=Homo sapiens, GI4505145, Length=351, Percent_Identity=26.7806267806268, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI270265879, Length=318, Percent_Identity=27.6729559748428, Blast_Score=71, Evalue=4e-12,
Organism=Escherichia coli, GI1788806, Length=752, Percent_Identity=58.7765957446808, Blast_Score=911, Evalue=0.0,
Organism=Escherichia coli, GI1788800, Length=317, Percent_Identity=32.1766561514196, Blast_Score=139, Evalue=1e-33,
Organism=Escherichia coli, GI1788635, Length=347, Percent_Identity=26.8011527377522, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI87081919, Length=294, Percent_Identity=25.8503401360544, Blast_Score=74, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17537199, Length=357, Percent_Identity=25.2100840336134, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322823, Length=235, Percent_Identity=27.6595744680851, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI281362674, Length=474, Percent_Identity=25.1054852320675, Blast_Score=87, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21356279, Length=474, Percent_Identity=25.1054852320675, Blast_Score=87, Evalue=5e-17,
Organism=Drosophila melanogaster, GI281362672, Length=474, Percent_Identity=25.1054852320675, Blast_Score=87, Evalue=6e-17,
Organism=Drosophila melanogaster, GI78707242, Length=437, Percent_Identity=24.4851258581236, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI78707238, Length=437, Percent_Identity=24.4851258581236, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI78707240, Length=346, Percent_Identity=25.4335260115607, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24646388, Length=350, Percent_Identity=26.5714285714286, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24646386, Length=350, Percent_Identity=26.5714285714286, Blast_Score=72, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.39 [H]

Molecular weight: Translated: 83788; Mature: 83657

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKPSSPSTRSDFDEAALFFHRYPKPGKLEIQATKPLGNQRDLALAYSPGVAAPCLAIH
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCEEEEE
DDPATAAEYTARGNLVAVVSNGTAVLGLGNIGALASKPVMEGKAVLFKKFADIDVFDIEI
CCCCCHHHHCCCCCEEEEEECCEEEEEECCCCCHHCCCCCCCHHHHHHHHCCCEEEEEEE
DAHEINRIVDVVSALEPTFGGINLEDIKAPECFEVEEQLRERMNIPVFHDDQHGTAIIVA
CHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEEH
SAVLNGLELAGKKIEEAKIVTSGAGAAALACLNLLVNLGAKRENIWVCDIEGVVYDGRNT
HHHHHHHHHHCCHHHHHEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEEECCEEECCHHH
LMDRWKEVYAQKTDARTLADVIGGADVFLGLSAAGVLKPELLKQMAEKPLIMALANPKPE
HHHHHHHHHHCCCCHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHCCCEEEEECCCCCC
IMPEEARAARADAMICTGRSDFPNQVNNVLCFPYIFRGALDVGATVINEEMKLAAVRAIA
CCCCHHHHHHCCEEEECCCCCCCHHHHCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLAREEPSDVAARAYSGDTPTFGPDYIIPSPFDQRLILRIAPAVAKAAMETGVATRPIED
HHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHH
MEAYLDRLNRFVFRSGLIMKPVFAAARTQGAPMRVIYADGEDERVLRAAQVVIEERIAAP
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCC
ILVGRPQVVETRLRRYGLKIRPGTDFELINPEDDPRYRDYVDLYLSYTGRQGVTPEAART
EEECCCHHHHHHHHHCCCEECCCCCEEEECCCCCCCHHHHHEEEEEECCCCCCCHHHHHH
IVRTNSTAIAALAVMRDEADAMICGLEGRFERHLRVVRQIIGKVPGIRDYSALSLLISQR
HHHCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
GALFLTDTYVNAEPDANEIAEMAVLAAREISRFGIEPKAALVSHSNFGSKESASAEKMRQ
CCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHEECCCCCCCCHHHHHHHHH
AAAILAEKAPDLESDGEMHGDAALSQTLRDRVFPSSRLKGEANLLVFPNLDAANITLNVV
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHCCCCCCEEEECCCCCCHHHHHHH
KAVTDALHVGPILLGATRPAHILTPSVTSRGVVNMTALAVVEALQKNASNRRQK
HHHHHHHHHCCEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKKPSSPSTRSDFDEAALFFHRYPKPGKLEIQATKPLGNQRDLALAYSPGVAAPCLAIH
CCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCEEEEE
DDPATAAEYTARGNLVAVVSNGTAVLGLGNIGALASKPVMEGKAVLFKKFADIDVFDIEI
CCCCCHHHHCCCCCEEEEEECCEEEEEECCCCCHHCCCCCCCHHHHHHHHCCCEEEEEEE
DAHEINRIVDVVSALEPTFGGINLEDIKAPECFEVEEQLRERMNIPVFHDDQHGTAIIVA
CHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEEH
SAVLNGLELAGKKIEEAKIVTSGAGAAALACLNLLVNLGAKRENIWVCDIEGVVYDGRNT
HHHHHHHHHHCCHHHHHEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEEECCEEECCHHH
LMDRWKEVYAQKTDARTLADVIGGADVFLGLSAAGVLKPELLKQMAEKPLIMALANPKPE
HHHHHHHHHHCCCCHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHCCCEEEEECCCCCC
IMPEEARAARADAMICTGRSDFPNQVNNVLCFPYIFRGALDVGATVINEEMKLAAVRAIA
CCCCHHHHHHCCEEEECCCCCCCHHHHCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLAREEPSDVAARAYSGDTPTFGPDYIIPSPFDQRLILRIAPAVAKAAMETGVATRPIED
HHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHH
MEAYLDRLNRFVFRSGLIMKPVFAAARTQGAPMRVIYADGEDERVLRAAQVVIEERIAAP
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCC
ILVGRPQVVETRLRRYGLKIRPGTDFELINPEDDPRYRDYVDLYLSYTGRQGVTPEAART
EEECCCHHHHHHHHHCCCEECCCCCEEEECCCCCCCHHHHHEEEEEECCCCCCCHHHHHH
IVRTNSTAIAALAVMRDEADAMICGLEGRFERHLRVVRQIIGKVPGIRDYSALSLLISQR
HHHCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
GALFLTDTYVNAEPDANEIAEMAVLAAREISRFGIEPKAALVSHSNFGSKESASAEKMRQ
CCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHEECCCCCCCCHHHHHHHHH
AAAILAEKAPDLESDGEMHGDAALSQTLRDRVFPSSRLKGEANLLVFPNLDAANITLNVV
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHCCCCCCEEEECCCCCCHHHHHHH
KAVTDALHVGPILLGATRPAHILTPSVTSRGVVNMTALAVVEALQKNASNRRQK
HHHHHHHHHCCEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9535928; 11481430; 10407149 [H]