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The map label for this gene is gltX [H]

Identifier: 225852521

GI number: 225852521

Start: 997587

End: 998960

Strand: Direct

Name: gltX [H]

Synonym: BMEA_A1057

Alternate gene names: 225852521

Gene position: 997587-998960 (Clockwise)

Preceding gene: 225852520

Following gene: 225852524

Centisome position: 46.93

GC content: 57.35

Gene sequence:

>1374_bases
ATGACTGTTACTGTCCGTTTTGCGCCGTCTCCCACCGGCTATATTCACATCGGAAATACGCGCACTGCGCTTTCCAACTG
GCTCTATGCCAGCAAGAACAATGGCAAATTCATCCTCCGTTACGACGATACGGACGTCGAGCGATCAAAGGACGAATATG
CACAGGCTATTGCGGTCGATCTCGACTGGCTGGGCGTCCGGCCGGACCGTGTGGAATATCAGTCGAAGCGTTTCGATATC
TACGCAAAAGCGGTGGAAAAGCTCAAAACGGCGGGCCTTCTCTATGCCTGCTACGAGACGGCAGACGAGCTTGAACGCCG
GCGCAAGTTGCGGCTTGCTCGCCGTTGGCCTCCGGTCTATGGCCGCGAGGCGCTGAAACTTACAGACGCTGAAAAAGCGG
CACTGGAGGCCGAAGGCCGCAAACCGCATTGGCGTTTCCTGCTGCCGAATTTCGAGAGCGATCCTTTTGCGACGCAGCGT
ACCGAGGTCCATTGGGACGATCTGGTGCGAGGTCCGCAAACGGTTGATCTTGCGTCCATGTCCGACCCGATCCTTGTGCG
CGAGGACGGCACCTATCTCTATACGCTGCCTTCGGTGGTTGATGACATCGACATGGGTGTGACCCATATTATTCGCGGCG
ACGACCATGTGACGAATACCGGCGTACAGATTTCGATATTCAAGGCGCTGGGTGCGACGCCTCCGGTTTTCGGCCACCAT
AATCTGCTCACGACCATATCGGGCGAAGGGCTTTCAAAGCGCACCGGCGCCTTGTCCGTCGGCTCCTTGCGTGAGGCAGG
CTATGAACCTATGGCCGTGGCGTCGCTGGCCATTCTTATCGGAACATCGGAAAGTGTCACTGCCGCCCCCGATATGGCGG
CGCTGGCCGAACATTTCGACCTTGCATCGATCTCCAAATCCTCTGCCAAATTCGATCCCTCGGAGCTTGATGCGCTGAAC
CGTTCGCTGCTGCATGAAATGCCGTTTGAAAAGGCAAAGCCACGTCTGGAAGCTCTGGGTATTTGTGGCGCAAAGGCCGA
ATCCTTCTGGCTTGCGGTTCGCGGCAATCTCGACCGTTTCAGCGATGTGTCCCATTGGTGGCAGGTGGTGAGCGGAGATT
TGCCGGAAGCGCCTGATCTTTCAGGCGAAGACCGCGATTTCGTGCGCCATGCCTTCGATTTGCTTCCGCCTGAACCATGG
AATGGGCAGACATGGAAAAGCTGGACCGAAGCGGTCAAATCCGCAACGGGCCGCAAGGGCAAAAACCTGTTCATGCCCTT
GCGTCTGGCGCTTACGGGGCAGGCGCATGGGCCGGAACTGGCTGATTTGCTGGTGCTGGTTGGTCTGGAAAGAACGAAGA
GCCGACGACCCTGA

Upstream 100 bases:

>100_bases
GGCAATCCACGCCCGGCGTCAAGATTACCAAGAAGAATTTCGGGCGGGATCGCCGTTATCCAATAACAAACCGCTTCCGC
GACCGCTGAAAGCATTGACA

Downstream 100 bases:

>100_bases
CATTTGAATTGCCCACATAGGGCCTTTCCGGCGGCGGCGCCACCGTCACCGGCTGAGCCTTGTTTTCTGCCGGTGCCGGC
AGGCTGTCCGAGAGCGAATT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1 [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MTVTVRFAPSPTGYIHIGNTRTALSNWLYASKNNGKFILRYDDTDVERSKDEYAQAIAVDLDWLGVRPDRVEYQSKRFDI
YAKAVEKLKTAGLLYACYETADELERRRKLRLARRWPPVYGREALKLTDAEKAALEAEGRKPHWRFLLPNFESDPFATQR
TEVHWDDLVRGPQTVDLASMSDPILVREDGTYLYTLPSVVDDIDMGVTHIIRGDDHVTNTGVQISIFKALGATPPVFGHH
NLLTTISGEGLSKRTGALSVGSLREAGYEPMAVASLAILIGTSESVTAAPDMAALAEHFDLASISKSSAKFDPSELDALN
RSLLHEMPFEKAKPRLEALGICGAKAESFWLAVRGNLDRFSDVSHWWQVVSGDLPEAPDLSGEDRDFVRHAFDLLPPEPW
NGQTWKSWTEAVKSATGRKGKNLFMPLRLALTGQAHGPELADLLVLVGLERTKSRRP

Sequences:

>Translated_457_residues
MTVTVRFAPSPTGYIHIGNTRTALSNWLYASKNNGKFILRYDDTDVERSKDEYAQAIAVDLDWLGVRPDRVEYQSKRFDI
YAKAVEKLKTAGLLYACYETADELERRRKLRLARRWPPVYGREALKLTDAEKAALEAEGRKPHWRFLLPNFESDPFATQR
TEVHWDDLVRGPQTVDLASMSDPILVREDGTYLYTLPSVVDDIDMGVTHIIRGDDHVTNTGVQISIFKALGATPPVFGHH
NLLTTISGEGLSKRTGALSVGSLREAGYEPMAVASLAILIGTSESVTAAPDMAALAEHFDLASISKSSAKFDPSELDALN
RSLLHEMPFEKAKPRLEALGICGAKAESFWLAVRGNLDRFSDVSHWWQVVSGDLPEAPDLSGEDRDFVRHAFDLLPPEPW
NGQTWKSWTEAVKSATGRKGKNLFMPLRLALTGQAHGPELADLLVLVGLERTKSRRP
>Mature_456_residues
TVTVRFAPSPTGYIHIGNTRTALSNWLYASKNNGKFILRYDDTDVERSKDEYAQAIAVDLDWLGVRPDRVEYQSKRFDIY
AKAVEKLKTAGLLYACYETADELERRRKLRLARRWPPVYGREALKLTDAEKAALEAEGRKPHWRFLLPNFESDPFATQRT
EVHWDDLVRGPQTVDLASMSDPILVREDGTYLYTLPSVVDDIDMGVTHIIRGDDHVTNTGVQISIFKALGATPPVFGHHN
LLTTISGEGLSKRTGALSVGSLREAGYEPMAVASLAILIGTSESVTAAPDMAALAEHFDLASISKSSAKFDPSELDALNR
SLLHEMPFEKAKPRLEALGICGAKAESFWLAVRGNLDRFSDVSHWWQVVSGDLPEAPDLSGEDRDFVRHAFDLLPPEPWN
GQTWKSWTEAVKSATGRKGKNLFMPLRLALTGQAHGPELADLLVLVGLERTKSRRP

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=500, Percent_Identity=29.4, Blast_Score=182, Evalue=4e-46,
Organism=Homo sapiens, GI62241042, Length=279, Percent_Identity=26.8817204301075, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI4826960, Length=157, Percent_Identity=35.031847133758, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1788743, Length=338, Percent_Identity=36.094674556213, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI226510925, Length=281, Percent_Identity=29.5373665480427, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1786895, Length=109, Percent_Identity=36.697247706422, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71993394, Length=494, Percent_Identity=29.1497975708502, Blast_Score=164, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI17543332, Length=103, Percent_Identity=36.8932038834951, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17511007, Length=113, Percent_Identity=31.858407079646, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324540, Length=490, Percent_Identity=29.3877551020408, Blast_Score=170, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6324742, Length=109, Percent_Identity=38.5321100917431, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6321192, Length=109, Percent_Identity=39.4495412844037, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24665321, Length=334, Percent_Identity=36.5269461077844, Blast_Score=187, Evalue=9e-48,
Organism=Drosophila melanogaster, GI17864488, Length=103, Percent_Identity=35.9223300970874, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24649466, Length=115, Percent_Identity=33.0434782608696, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 50867; Mature: 50736

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTVRFAPSPTGYIHIGNTRTALSNWLYASKNNGKFILRYDDTDVERSKDEYAQAIAVD
CEEEEEECCCCCCEEEECCCHHHHHHCEEEECCCCEEEEEECCCCCCCCHHHHHHEEEEE
LDWLGVRPDRVEYQSKRFDIYAKAVEKLKTAGLLYACYETADELERRRKLRLARRWPPVY
EHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GREALKLTDAEKAALEAEGRKPHWRFLLPNFESDPFATQRTEVHWDDLVRGPQTVDLASM
CCCCEEECCHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCEEECCC
SDPILVREDGTYLYTLPSVVDDIDMGVTHIIRGDDHVTNTGVQISIFKALGATPPVFGHH
CCCEEEEECCCEEEECCHHHHHHHCCCEEEECCCCCEECCCEEEEEEHHHCCCCCCCCCC
NLLTTISGEGLSKRTGALSVGSLREAGYEPMAVASLAILIGTSESVTAAPDMAALAEHFD
CEEEEECCCCCCCCCCCEECCHHHHCCCCCHHHHEEEEEEECCCCCCCCCCHHHHHHHCC
LASISKSSAKFDPSELDALNRSLLHEMPFEKAKPRLEALGICGAKAESFWLAVRGNLDRF
HHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCHHHHCCCCCCCCEEEEEEECCCHHH
SDVSHWWQVVSGDLPEAPDLSGEDRDFVRHAFDLLPPEPWNGQTWKSWTEAVKSATGRKG
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCC
KNLFMPLRLALTGQAHGPELADLLVLVGLERTKSRRP
CCEEEEEEEEEECCCCCCHHHHHHHHHCCHHHCCCCC
>Mature Secondary Structure 
TVTVRFAPSPTGYIHIGNTRTALSNWLYASKNNGKFILRYDDTDVERSKDEYAQAIAVD
EEEEEECCCCCCEEEECCCHHHHHHCEEEECCCCEEEEEECCCCCCCCHHHHHHEEEEE
LDWLGVRPDRVEYQSKRFDIYAKAVEKLKTAGLLYACYETADELERRRKLRLARRWPPVY
EHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GREALKLTDAEKAALEAEGRKPHWRFLLPNFESDPFATQRTEVHWDDLVRGPQTVDLASM
CCCCEEECCHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCEEECCC
SDPILVREDGTYLYTLPSVVDDIDMGVTHIIRGDDHVTNTGVQISIFKALGATPPVFGHH
CCCEEEEECCCEEEECCHHHHHHHCCCEEEECCCCCEECCCEEEEEEHHHCCCCCCCCCC
NLLTTISGEGLSKRTGALSVGSLREAGYEPMAVASLAILIGTSESVTAAPDMAALAEHFD
CEEEEECCCCCCCCCCCEECCHHHHCCCCCHHHHEEEEEEECCCCCCCCCCHHHHHHHCC
LASISKSSAKFDPSELDALNRSLLHEMPFEKAKPRLEALGICGAKAESFWLAVRGNLDRF
HHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCHHHHCCCCCCCCEEEEEEECCCHHH
SDVSHWWQVVSGDLPEAPDLSGEDRDFVRHAFDLLPPEPWNGQTWKSWTEAVKSATGRKG
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCC
KNLFMPLRLALTGQAHGPELADLLVLVGLERTKSRRP
CCEEEEEEEEEECCCCCCHHHHHHHHHCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA