Click here to switch to the map view.

The map label for this gene is nadE [H]

Identifier: 225852520

GI number: 225852520

Start: 995896

End: 997575

Strand: Direct

Name: nadE [H]

Synonym: BMEA_A1056

Alternate gene names: 225852520

Gene position: 995896-997575 (Clockwise)

Preceding gene: 225852519

Following gene: 225852521

Centisome position: 46.85

GC content: 56.9

Gene sequence:

>1680_bases
ATGACAGGCTCTCACGAGAATCTCACCATGCTTCGTATTGCGGTTGCGCAGCTCAATCCCACCATGGGCGATATCGCCGG
AAATCTGGCCAAAGCACGGACGGCCCGCGCCGAAGCGGCAAGGATGCAGGCCGATCTTATCCTTTTTACGGAACTTTTCA
TTTCCGGCTATCCGCCGGAAGACATGGTTTTGAAACCTGCTTTTGTTGCCGCCTGCGAGAAGGCCGTGCGTATGCTTGCC
GCCGACACAGCCGATGGCGGCCCCGGTGTCATCATCGGCACGCCGCTGCACCGCGATAGCGGTTCGCATAATGCTGTTGC
CGTTCTGGATGGCGGGGAGATTATCGCTGAGCGTTTCAAGGTCGATCTGCCAAATTATGGCGAATTCGATGAAAAGCGCG
TGTTCGAGCCCGGCCCGATGCCCGGCCCGGTTAATTTTCGAGGTGTGCGGCTCGGCATTCCCGTCTGTGAAGACATATGG
GGCGAATTGGGTGTAGCGGAAACGCTGGCCGAAAGCGGTGCGGAAATCCTGCTTGTGCCGAATGGCTCGCCTTATCATCG
CGCCAAGATGGACCGCCGCCATCAGGTGGTGCTGAAACAGGTCATCGAGACCGGACTGCCGATCGTCTATGCCAATCAGG
TCGGCGGGCAGGATGAGCTGGTCTTCGATGGCGGCTCCTTTGTTTTCAATGCGGATAAATCGCTTTGCCTGCAAATGCCG
CAATTCGCCGAACAGATTGCGTTGACTGAGTGGCAACGGGATGGTGACGGATGGCGTTGCAGGGATGGCGAAAAGGCAAG
CCTGCCGGAAGGGCTGGCAGCCGATTATGCCGCCTGTATGCTTGGCTTGCGCGATTATGTGAACAAGAACGGTTTCAAGG
ATGTCGTACTGGGCCTTTCCGGCGGCATTGACTCTGCCATCTGTGCGGCACTTGGTGTCGATGCCCTGGGCAAAGACCGT
GTTCGCTGTGTGATGCTTCCCTATCGCTATACTTCGGAGGAATCGCTCGCGGATGCCGCCGATTGCGCCAAGGCGCTTGG
CGTGCGCTACGATATCGTTCCGATTGCCGAACCGGTCGAAGGCTTCATGAATGCGCTGCAGCCGCTTTTTTCCGGAACGG
ACAGCGGCGTGACCGAAGAAAACCTTCAGAGCCGTGCGCGCGGCACGATCCTGATGGCAATCTCGAACAAATTCGGGTCG
ATGGTGGTTACGACCGGCAACAAGTCCGAAATGTCGGTTGGTTATGCCACGCTTTATGGCGACATGAATGGCGGCTTCAA
TCCGATCAAGGACGTGTACAAGATGCAGGTCTATGCGCTGTCGCAATGGCGCAACCAGAATCTGCCGCAGGGCGCACTTG
GCCCGGCGGGTGAGGTGATCCCGCAAAATATCATCTCGAAAGCGCCTTCTGCCGAATTGCGCGAAAACCAGACCGATCAG
GATTCGCTGCCGCCTTATCCTGTTCTGGATGATATTCTGGAATGCCTGGTCGAAAACGAGATGAGCAATGCTGAAATCGT
TGCGCGCGGCCATGCGCTGGAAACCGTGCAACGCATTGAACACCTTCTCTATCTCGCAGAATATAAGCGCCGGCAATCCA
CGCCCGGCGTCAAGATTACCAAGAAGAATTTCGGGCGGGATCGCCGTTATCCAATAACAAACCGCTTCCGCGACCGCTGA

Upstream 100 bases:

>100_bases
TGGGCCTATATTATTGCGACTACCTATCTGGCCTGATAAACCGCGCGGTTCCTTACAGCATCCGTTGCAATCGATTGCAT
AAGCGCGCTAAAGCTGTCGC

Downstream 100 bases:

>100_bases
AAGCATTGACAATGACTGTTACTGTCCGTTTTGCGCCGTCTCCCACCGGCTATATTCACATCGGAAATACGCGCACTGCG
CTTTCCAACTGGCTCTATGC

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 559; Mature: 558

Protein sequence:

>559_residues
MTGSHENLTMLRIAVAQLNPTMGDIAGNLAKARTARAEAARMQADLILFTELFISGYPPEDMVLKPAFVAACEKAVRMLA
ADTADGGPGVIIGTPLHRDSGSHNAVAVLDGGEIIAERFKVDLPNYGEFDEKRVFEPGPMPGPVNFRGVRLGIPVCEDIW
GELGVAETLAESGAEILLVPNGSPYHRAKMDRRHQVVLKQVIETGLPIVYANQVGGQDELVFDGGSFVFNADKSLCLQMP
QFAEQIALTEWQRDGDGWRCRDGEKASLPEGLAADYAACMLGLRDYVNKNGFKDVVLGLSGGIDSAICAALGVDALGKDR
VRCVMLPYRYTSEESLADAADCAKALGVRYDIVPIAEPVEGFMNALQPLFSGTDSGVTEENLQSRARGTILMAISNKFGS
MVVTTGNKSEMSVGYATLYGDMNGGFNPIKDVYKMQVYALSQWRNQNLPQGALGPAGEVIPQNIISKAPSAELRENQTDQ
DSLPPYPVLDDILECLVENEMSNAEIVARGHALETVQRIEHLLYLAEYKRRQSTPGVKITKKNFGRDRRYPITNRFRDR

Sequences:

>Translated_559_residues
MTGSHENLTMLRIAVAQLNPTMGDIAGNLAKARTARAEAARMQADLILFTELFISGYPPEDMVLKPAFVAACEKAVRMLA
ADTADGGPGVIIGTPLHRDSGSHNAVAVLDGGEIIAERFKVDLPNYGEFDEKRVFEPGPMPGPVNFRGVRLGIPVCEDIW
GELGVAETLAESGAEILLVPNGSPYHRAKMDRRHQVVLKQVIETGLPIVYANQVGGQDELVFDGGSFVFNADKSLCLQMP
QFAEQIALTEWQRDGDGWRCRDGEKASLPEGLAADYAACMLGLRDYVNKNGFKDVVLGLSGGIDSAICAALGVDALGKDR
VRCVMLPYRYTSEESLADAADCAKALGVRYDIVPIAEPVEGFMNALQPLFSGTDSGVTEENLQSRARGTILMAISNKFGS
MVVTTGNKSEMSVGYATLYGDMNGGFNPIKDVYKMQVYALSQWRNQNLPQGALGPAGEVIPQNIISKAPSAELRENQTDQ
DSLPPYPVLDDILECLVENEMSNAEIVARGHALETVQRIEHLLYLAEYKRRQSTPGVKITKKNFGRDRRYPITNRFRDR
>Mature_558_residues
TGSHENLTMLRIAVAQLNPTMGDIAGNLAKARTARAEAARMQADLILFTELFISGYPPEDMVLKPAFVAACEKAVRMLAA
DTADGGPGVIIGTPLHRDSGSHNAVAVLDGGEIIAERFKVDLPNYGEFDEKRVFEPGPMPGPVNFRGVRLGIPVCEDIWG
ELGVAETLAESGAEILLVPNGSPYHRAKMDRRHQVVLKQVIETGLPIVYANQVGGQDELVFDGGSFVFNADKSLCLQMPQ
FAEQIALTEWQRDGDGWRCRDGEKASLPEGLAADYAACMLGLRDYVNKNGFKDVVLGLSGGIDSAICAALGVDALGKDRV
RCVMLPYRYTSEESLADAADCAKALGVRYDIVPIAEPVEGFMNALQPLFSGTDSGVTEENLQSRARGTILMAISNKFGSM
VVTTGNKSEMSVGYATLYGDMNGGFNPIKDVYKMQVYALSQWRNQNLPQGALGPAGEVIPQNIISKAPSAELRENQTDQD
SLPPYPVLDDILECLVENEMSNAEIVARGHALETVQRIEHLLYLAEYKRRQSTPGVKITKKNFGRDRRYPITNRFRDR

Specific function: It is not known if this NAD synthase uses NH(3) or glutamine as the N donor [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 60920; Mature: 60789

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGSHENLTMLRIAVAQLNPTMGDIAGNLAKARTARAEAARMQADLILFTELFISGYPPE
CCCCCCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DMVLKPAFVAACEKAVRMLAADTADGGPGVIIGTPLHRDSGSHNAVAVLDGGEIIAERFK
CCEECHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCEEEEECCHHHHHHHHH
VDLPNYGEFDEKRVFEPGPMPGPVNFRGVRLGIPVCEDIWGELGVAETLAESGAEILLVP
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHCCHHHHHHHCCCEEEEEC
NGSPYHRAKMDRRHQVVLKQVIETGLPIVYANQVGGQDELVFDGGSFVFNADKSLCLQMP
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEECCCEEEECCHHHHHHHH
QFAEQIALTEWQRDGDGWRCRDGEKASLPEGLAADYAACMLGLRDYVNKNGFKDVVLGLS
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHEEEECC
GGIDSAICAALGVDALGKDRVRCVMLPYRYTSEESLADAADCAKALGVRYDIVPIAEPVE
CCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEECCHHHH
GFMNALQPLFSGTDSGVTEENLQSRARGTILMAISNKFGSMVVTTGNKSEMSVGYATLYG
HHHHHHHHHHCCCCCCCCHHHHHHHHCCEEEEEEECCCCCEEEEECCCCCCEEEEEEEEE
DMNGGFNPIKDVYKMQVYALSQWRNQNLPQGALGPAGEVIPQNIISKAPSAELRENQTDQ
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHCCCCCC
DSLPPYPVLDDILECLVENEMSNAEIVARGHALETVQRIEHLLYLAEYKRRQSTPGVKIT
CCCCCCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
KKNFGRDRRYPITNRFRDR
CCCCCCCCCCCCCHHCCCC
>Mature Secondary Structure 
TGSHENLTMLRIAVAQLNPTMGDIAGNLAKARTARAEAARMQADLILFTELFISGYPPE
CCCCCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DMVLKPAFVAACEKAVRMLAADTADGGPGVIIGTPLHRDSGSHNAVAVLDGGEIIAERFK
CCEECHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCEEEEECCHHHHHHHHH
VDLPNYGEFDEKRVFEPGPMPGPVNFRGVRLGIPVCEDIWGELGVAETLAESGAEILLVP
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHCCHHHHHHHCCCEEEEEC
NGSPYHRAKMDRRHQVVLKQVIETGLPIVYANQVGGQDELVFDGGSFVFNADKSLCLQMP
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEECCCEEEECCHHHHHHHH
QFAEQIALTEWQRDGDGWRCRDGEKASLPEGLAADYAACMLGLRDYVNKNGFKDVVLGLS
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHEEEECC
GGIDSAICAALGVDALGKDRVRCVMLPYRYTSEESLADAADCAKALGVRYDIVPIAEPVE
CCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEECCHHHH
GFMNALQPLFSGTDSGVTEENLQSRARGTILMAISNKFGSMVVTTGNKSEMSVGYATLYG
HHHHHHHHHHCCCCCCCCHHHHHHHHCCEEEEEEECCCCCEEEEECCCCCCEEEEEEEEE
DMNGGFNPIKDVYKMQVYALSQWRNQNLPQGALGPAGEVIPQNIISKAPSAELRENQTDQ
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHCCCCCC
DSLPPYPVLDDILECLVENEMSNAEIVARGHALETVQRIEHLLYLAEYKRRQSTPGVKIT
CCCCCCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
KKNFGRDRRYPITNRFRDR
CCCCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8431308; 8195100 [H]