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The map label for this gene is metG [H]

Identifier: 225852500

GI number: 225852500

Start: 977080

End: 978627

Strand: Direct

Name: metG [H]

Synonym: BMEA_A1036

Alternate gene names: 225852500

Gene position: 977080-978627 (Clockwise)

Preceding gene: 225852499

Following gene: 225852501

Centisome position: 45.97

GC content: 57.82

Gene sequence:

>1548_bases
ATGAGCCGCGAAAAATATTACATCACGACTGCGATTGCTTATCCGAACGGAAAGCCGCATATCGGCCACGCTTACGAACT
GATCGCGACCGATGCCATGGCGCGGTTTCAGCGTCTCAATGGCATGGATGTTTATTTTCTGACCGGCACGGACGAGCATG
GCATCAAGATGCTGCAAAGCGCGCGCAAGGAAGGCATTACGCCGCGCGAGCTGGCAGACCGCAACACATCCGCCTTCCGT
CGAATGGCTGAAGTGCTGAACAGCTCCAACGACGACTATATCCGCACCTCCGAAGAGCGTCATTACAAGGCCAGCCAGGT
CATTTGGCAGGCGATGGTCGCAAATGGGGATATTTACAAGGGCGGCTATGCCGGCTGGTATTCCGTGCGCGACGAAGCCT
ATTACGGCGAGGAAGAAACGGAAGTGCGCGCGGATGGCGTCCGCTACGGCCCGCAGGGAACGCCTGTGGAATGGGTGGAG
GAGGAAAGCTATTTCTTCCGTCTTTCGGCCTATCAGGACAAGCTGCTCGACCTCTACGAGAACAATCCGGGCTTTATCAT
GCCCGCCGAACGCCGCAATGAGATTGTGAGCTTCGTGAAGTCAGGTCTCAAGGACCTGTCGATTTCGCGCACGACCTTCG
ATTGGGGCATTCCGGTGCCGGGAGATGAAAAGCACGTCATGTATGTGTGGGTGGATGCGCTCACCAATTACATCACTGCG
CTCGGCTATCCTGATACGACCGATGAGCGTTGGGCTTACTGGCCCGCCAATGCCCATATCATCGGCAAGGATATTTCCCG
CTTCCATGCGGTCTATTGGCCTGCATTCCTGATGTCGGCCCAATTACCCTTGCCGAAGCGTGTTTTCGCGCATGGGTTCC
TGTTCAATCGCGGCGAGAAAATGTCGAAGTCAGTCGGCAATGTTATCGATCCGTTTGAACTGGTGGAGCGTTACGGCCTG
GACCAGTTGCGTTATTTCCTGATGCGTGAAGTGCCATTCGGCCAGGATGGCAGCTACAGCCATGAGGCGATCGTCAACCG
CACCAATGCCGATCTGGCCAACGACCTCGGCAATCTTGCCCAGCGTTCGCTATCGATGATCGCCAAGAATTGCGAAGGCA
AGGTGCCGCAGCCCGGCGCATTTTCCGAGGCTGACAAGGCCATTCTGGATCAGGCCGATGCCGCGCTCGAAACCGCGCGC
AAGGCAATGGACGATCAGGCCCTGCATCTGGCATTGGGTGCGATCTTCGCTGTTGTGGCGGAAGCCAACCGCTATTTTGC
GGGGCAGGAGCCATGGGCACTGCGCAAGACCGATCCTGCGCGCATGGGCACCGTGCTCTATGTAACAGCCGAGGTTCTGC
GCCGCGTCGGCATCATGGTGCAGCCTTTCATCCCGCAATCTGCGGAGAAGCTGCTCGATATTCTGGCTGTCCCGGCTGAC
AAGCGCCAGTTTGCCGATGTGCTGGCAAGCCCGCTTGCTGGTGGGACCGATCTGCCTGCGCCGCAGCCGGTCTTCCCGCG
CTATGTGGAAGCGGACGAACAGAACTGA

Upstream 100 bases:

>100_bases
ATGTCACCGCTGACGAATTTCTTCTCGCGCGCGTACCACGCATTGCTTCGGGGCATTGCTTCGGGCGCGCGCTTCCAAAC
ATCGAAAGACCGGGTCCATA

Downstream 100 bases:

>100_bases
GTTTGATGATGCTTGTCGATAGCCATTGCCACCTCGATTTTGCCGATTTTGAGCCGGAGCGCGATGCTGTTGTTCAGCGC
GCGCTCGATGCGGGCATCAA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MSREKYYITTAIAYPNGKPHIGHAYELIATDAMARFQRLNGMDVYFLTGTDEHGIKMLQSARKEGITPRELADRNTSAFR
RMAEVLNSSNDDYIRTSEERHYKASQVIWQAMVANGDIYKGGYAGWYSVRDEAYYGEEETEVRADGVRYGPQGTPVEWVE
EESYFFRLSAYQDKLLDLYENNPGFIMPAERRNEIVSFVKSGLKDLSISRTTFDWGIPVPGDEKHVMYVWVDALTNYITA
LGYPDTTDERWAYWPANAHIIGKDISRFHAVYWPAFLMSAQLPLPKRVFAHGFLFNRGEKMSKSVGNVIDPFELVERYGL
DQLRYFLMREVPFGQDGSYSHEAIVNRTNADLANDLGNLAQRSLSMIAKNCEGKVPQPGAFSEADKAILDQADAALETAR
KAMDDQALHLALGAIFAVVAEANRYFAGQEPWALRKTDPARMGTVLYVTAEVLRRVGIMVQPFIPQSAEKLLDILAVPAD
KRQFADVLASPLAGGTDLPAPQPVFPRYVEADEQN

Sequences:

>Translated_515_residues
MSREKYYITTAIAYPNGKPHIGHAYELIATDAMARFQRLNGMDVYFLTGTDEHGIKMLQSARKEGITPRELADRNTSAFR
RMAEVLNSSNDDYIRTSEERHYKASQVIWQAMVANGDIYKGGYAGWYSVRDEAYYGEEETEVRADGVRYGPQGTPVEWVE
EESYFFRLSAYQDKLLDLYENNPGFIMPAERRNEIVSFVKSGLKDLSISRTTFDWGIPVPGDEKHVMYVWVDALTNYITA
LGYPDTTDERWAYWPANAHIIGKDISRFHAVYWPAFLMSAQLPLPKRVFAHGFLFNRGEKMSKSVGNVIDPFELVERYGL
DQLRYFLMREVPFGQDGSYSHEAIVNRTNADLANDLGNLAQRSLSMIAKNCEGKVPQPGAFSEADKAILDQADAALETAR
KAMDDQALHLALGAIFAVVAEANRYFAGQEPWALRKTDPARMGTVLYVTAEVLRRVGIMVQPFIPQSAEKLLDILAVPAD
KRQFADVLASPLAGGTDLPAPQPVFPRYVEADEQN
>Mature_514_residues
SREKYYITTAIAYPNGKPHIGHAYELIATDAMARFQRLNGMDVYFLTGTDEHGIKMLQSARKEGITPRELADRNTSAFRR
MAEVLNSSNDDYIRTSEERHYKASQVIWQAMVANGDIYKGGYAGWYSVRDEAYYGEEETEVRADGVRYGPQGTPVEWVEE
ESYFFRLSAYQDKLLDLYENNPGFIMPAERRNEIVSFVKSGLKDLSISRTTFDWGIPVPGDEKHVMYVWVDALTNYITAL
GYPDTTDERWAYWPANAHIIGKDISRFHAVYWPAFLMSAQLPLPKRVFAHGFLFNRGEKMSKSVGNVIDPFELVERYGLD
QLRYFLMREVPFGQDGSYSHEAIVNRTNADLANDLGNLAQRSLSMIAKNCEGKVPQPGAFSEADKAILDQADAALETARK
AMDDQALHLALGAIFAVVAEANRYFAGQEPWALRKTDPARMGTVLYVTAEVLRRVGIMVQPFIPQSAEKLLDILAVPADK
RQFADVLASPLAGGTDLPAPQPVFPRYVEADEQN

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=527, Percent_Identity=37.3814041745731, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI14043022, Length=511, Percent_Identity=25.8317025440313, Blast_Score=148, Evalue=2e-35,
Organism=Homo sapiens, GI7661872, Length=186, Percent_Identity=28.494623655914, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788432, Length=510, Percent_Identity=26.4705882352941, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1786861, Length=178, Percent_Identity=26.9662921348315, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI212645096, Length=513, Percent_Identity=35.8674463937622, Blast_Score=335, Evalue=4e-92,
Organism=Caenorhabditis elegans, GI71992781, Length=403, Percent_Identity=35.4838709677419, Blast_Score=258, Evalue=6e-69,
Organism=Caenorhabditis elegans, GI17540794, Length=557, Percent_Identity=25.8527827648115, Blast_Score=147, Evalue=9e-36,
Organism=Saccharomyces cerevisiae, GI6321610, Length=529, Percent_Identity=31.5689981096408, Blast_Score=282, Evalue=7e-77,
Organism=Saccharomyces cerevisiae, GI6321704, Length=516, Percent_Identity=23.4496124031008, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21358483, Length=495, Percent_Identity=37.7777777777778, Blast_Score=340, Evalue=1e-93,
Organism=Drosophila melanogaster, GI24655311, Length=555, Percent_Identity=26.1261261261261, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI21355409, Length=183, Percent_Identity=28.9617486338798, Blast_Score=79, Evalue=8e-15,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 58026; Mature: 57895

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSREKYYITTAIAYPNGKPHIGHAYELIATDAMARFQRLNGMDVYFLTGTDEHGIKMLQS
CCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHH
ARKEGITPRELADRNTSAFRRMAEVLNSSNDDYIRTSEERHYKASQVIWQAMVANGDIYK
HHHCCCCHHHHHCCCHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHHHCCCEEC
GGYAGWYSVRDEAYYGEEETEVRADGVRYGPQGTPVEWVEEESYFFRLSAYQDKLLDLYE
CCCCCEEEECCCCCCCCCHHHHHHCCEEECCCCCCHHHEECCCEEEEEHHHHHHHHHHHC
NNPGFIMPAERRNEIVSFVKSGLKDLSISRTTFDWGIPVPGDEKHVMYVWVDALTNYITA
CCCCEEEEHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEEHHHHHHHHHH
LGYPDTTDERWAYWPANAHIIGKDISRFHAVYWPAFLMSAQLPLPKRVFAHGFLFNRGEK
CCCCCCCCCCEEECCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHH
MSKSVGNVIDPFELVERYGLDQLRYFLMREVPFGQDGSYSHEAIVNRTNADLANDLGNLA
HHHHHCCCCCHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHH
QRSLSMIAKNCEGKVPQPGAFSEADKAILDQADAALETARKAMDDQALHLALGAIFAVVA
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EANRYFAGQEPWALRKTDPARMGTVLYVTAEVLRRVGIMVQPFIPQSAEKLLDILAVPAD
HHHHHCCCCCCCEECCCCHHHHCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHCCCCC
KRQFADVLASPLAGGTDLPAPQPVFPRYVEADEQN
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SREKYYITTAIAYPNGKPHIGHAYELIATDAMARFQRLNGMDVYFLTGTDEHGIKMLQS
CCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHH
ARKEGITPRELADRNTSAFRRMAEVLNSSNDDYIRTSEERHYKASQVIWQAMVANGDIYK
HHHCCCCHHHHHCCCHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHHHCCCEEC
GGYAGWYSVRDEAYYGEEETEVRADGVRYGPQGTPVEWVEEESYFFRLSAYQDKLLDLYE
CCCCCEEEECCCCCCCCCHHHHHHCCEEECCCCCCHHHEECCCEEEEEHHHHHHHHHHHC
NNPGFIMPAERRNEIVSFVKSGLKDLSISRTTFDWGIPVPGDEKHVMYVWVDALTNYITA
CCCCEEEEHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEEHHHHHHHHHH
LGYPDTTDERWAYWPANAHIIGKDISRFHAVYWPAFLMSAQLPLPKRVFAHGFLFNRGEK
CCCCCCCCCCEEECCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHH
MSKSVGNVIDPFELVERYGLDQLRYFLMREVPFGQDGSYSHEAIVNRTNADLANDLGNLA
HHHHHCCCCCHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHH
QRSLSMIAKNCEGKVPQPGAFSEADKAILDQADAALETARKAMDDQALHLALGAIFAVVA
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EANRYFAGQEPWALRKTDPARMGTVLYVTAEVLRRVGIMVQPFIPQSAEKLLDILAVPAD
HHHHHCCCCCCCEECCCCHHHHCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHCCCCC
KRQFADVLASPLAGGTDLPAPQPVFPRYVEADEQN
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]