| Definition | Brucella melitensis ATCC 23457 chromosome chromosome I, complete sequence. |
|---|---|
| Accession | NC_012441 |
| Length | 2,125,701 |
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The map label for this gene is rne [H]
Identifier: 225852424
GI number: 225852424
Start: 904850
End: 907525
Strand: Reverse
Name: rne [H]
Synonym: BMEA_A0951
Alternate gene names: 225852424
Gene position: 907525-904850 (Counterclockwise)
Preceding gene: 225852428
Following gene: 225852422
Centisome position: 42.69
GC content: 58.86
Gene sequence:
>2676_bases ATGTCCAACAAAATGCTTATAGATGCCTCCCACCCGGAGGAGACCCGCGTTATCGTCACGCGCGGCAACAAGATTGAAGA ATTCGATTTCGAATCGGAACATAAGAAACAGCTGAAAGGCAATATCTACCTCGCACGCGTCACCCGTGTCGAACCGTCGC TTCAGGCTGCATTCGTGGAATATGGCGGCAATCGCCATGGCTTCCTGGCATTTTCCGAAATTCATCCCGACTATTACCAG ATTCCGGTCGCCGACCGCCTTGCCCTGCTTGAAGCGGAAGCCAAGGCCGCACGCGCTGAAGACGACGAGGACGAACCGCG CGCCAAAAGCGACCGCAGTGAACGCAACGACCGCGCGCGCCGCAATCGCCGTCGTGGCGGCCGCCGCGATGGCCAGAATG GCGAAGCGCAGGCAAACGGTGCTGACAAGGACCTGCCGGCGGCAGAGCCGGTCGGTCCGGGCTTTGCGGATTACGTTGCG GGAACGCCCGCCGCTGCTCTCACGCAGAAAGGCGATATAATTTCCGAAGCCCTCGACAATGGCGATGACGATGATGTCGA GAACGAAGAGGACATGGTCGAATCGGTCGGCTCGGAAGATGCTCTGGAAGAGCTTCCGACCCACACCCGCACCCATCGCC GCCAGTATAATATTCAGGAAGTCATCAAGCGCCGCCAGATTCTTCTGGTGCAGGTGGTCAAGGAAGAGCGCGGCAACAAG GGCGCTGCTCTCACCACCTATCTTTCGCTTGCCGGACGTTATTCGGTACTGATGCCCAACACCGCGCGCGGTGGCGGCAT TTCGCGCAAGATCACCAATCCGCAGGACCGCAAGCGCCTGAAAGAGATCGTGAAGGAACTCGAAGTGCCGCAGGGCATGG GTGTGATCCTGCGCACGGCAGGCGCAAACCGCACCAAGGCGGAAGTCAAGCGCGACTATGAATATCTGATGCGCCTGTGG GAAAACGTGCGCACGCTGACGCTGCAATCGACAGCGCCCGCGCTCGTCTATGAAGAAGGCAGCCTCATCAAGCGCTCTAT CCGTGATATTTACAACAAGGACATCACGGAAATACTCGTTTCCGGCGAGAACGGCTATCGCGAGGCCAAGGATTTCATGC GTATGCTCATGCCGAGCCATGCGAAGGTCGTCCAGCCTTACCGCGATCATATTCCGATCTTCACGCGCAACGGCGTGGAA GCGCAGCTTGACCGTATGCTTGTGCCGCAGGTGACGCTCAAATCGGGCGGCTACCTCATCATCAACCAGACGGAAGCCCT GGTTGCCATCGACGTCAATTCGGGCCGCTCGACCCGCGAACATTCGATTGAGGACACCGCGCTTCAGACCAATCTGGAAG CAGCCGAAGAAGTTGCGCGCCAGTTGCGCCTTCGCGACCTTGCGGGCCTGATCGTCGTTGACTTCATCGACATGGAAGAA AAGCGCAACAATCGCGCTGTCGAAAAGAAGATGAAGGAATGCCTGAAGGATGACCGCGCCCGCATCCAGGTGGGCCGCAT CTCGCATTTCGGCCTTCTGGAAATGTCGCGCCAGCGCATCCGCGCATCGGTTCTGGAAAGCACCATGCAGGTCTGCCCGC ATTGCGGCGGCACCGGACATATCCGTTCGGATTCCTCCATGGCGCTCCATATCATCCGTGGTATCGAAGACTATCTGCTG CGCCATTCGGGCTTTGATATTCATGTCCGCACCCCGGCAGCGTCCGCACTTTATGTGCTCAATCACAAGCGCCAGATTCT GGCCGATCTGGAGGGGCGCTTTGGCGTCGAGATCAGCATTGATGCCGACGAAAGTGTAGGAAACCAGCACTTTGTCATTG ATAAGGGTGCGCCTTCTACCCGCCCCGTCACCCCGTCGGCAGTACAGCCGATGGTCTATGAGGACGATATCGAAGACCCT GAAATCCCGGTCGAAGAAGACGAGACCGAAGAAGAAGCAAGGACCGATGTTCAGGCTGGCGAAGAGCGCAGCGAAGAAAA CGACCGCAAGCGCCGCCGTCGTCGCCGTCGTCGCGGTGGCCGCGAACGTGACGGTCGTGATCTGTCCGCGCAGGAAGAAG CCGTTTCGGAGGAAGCATCCGAAGAGGATAATGAAACCGATCAGGCCAATGAGGCCGTGTCATCCGCTTCCATGAGCGAG GAAGATCGCCGCAAGAAGCGTCGTCGCGGCCGCCGTGGCGGACGGAAGAACCGGCGTGAAGACGACAACCGTGCGCGCAG AATTCCTGATCCGGAATTCGTCGGTTACACACCTGTTCTGCCGGTAAAGGCCGAAGAAAGCATCGGCGAAGTAGTGGAAA CGGTTGAAACCGTGGAAGCACCTGTCGTTGAGGCCGCGCCGGAAGAAAAGCCTGAAAAGCCCGCCCGCAAAACGCGCACG CGCAAGAAGGCTGTCGACGAAGCGCCTGCAGATGAAGCCGCAGCGCCCGCCGAGGAAGTGGTACCTGAAAAGCCGAAGCG CCGTACACGCAAAGCTGCCGCCAAGCCGGTTGTGGAAGCCGAAGACGTTGCCGAGGTGAAGGAAACGGTTGCGGCAGAAA CGCAGGTGGAAACCATCGCGGAACCGAAAAGCACAGAGCCGGTCGTAACCTCCAGCGAGAGCGAAGACCGGAAGCCGAAG CGTTCGGGCTGGTGGCAGAAGAAGGGCTTTTTCTAA
Upstream 100 bases:
>100_bases CCAGAATCATCCGGCAGATGCATCGCAGTCAGGACAAAAGCCTTCAGGGCTTCGTCCGGCGCACGCGGCTTGCGTCTTCG CGCCGGCAGGAAAGACAATA
Downstream 100 bases:
>100_bases GAAGCCGGGCCGCCTTTCCGCTGCCCTGTTACGGATGACAAAAAACCGGCGGAAACGCCGGTTTTTCTTTATGTTTTTGC CGCAGAACGGCTCCCTTTCA
Product: ribonuclease, Rne/Rng family protein
Products: NA
Alternate protein names: RNase E [H]
Number of amino acids: Translated: 891; Mature: 890
Protein sequence:
>891_residues MSNKMLIDASHPEETRVIVTRGNKIEEFDFESEHKKQLKGNIYLARVTRVEPSLQAAFVEYGGNRHGFLAFSEIHPDYYQ IPVADRLALLEAEAKAARAEDDEDEPRAKSDRSERNDRARRNRRRGGRRDGQNGEAQANGADKDLPAAEPVGPGFADYVA GTPAAALTQKGDIISEALDNGDDDDVENEEDMVESVGSEDALEELPTHTRTHRRQYNIQEVIKRRQILLVQVVKEERGNK GAALTTYLSLAGRYSVLMPNTARGGGISRKITNPQDRKRLKEIVKELEVPQGMGVILRTAGANRTKAEVKRDYEYLMRLW ENVRTLTLQSTAPALVYEEGSLIKRSIRDIYNKDITEILVSGENGYREAKDFMRMLMPSHAKVVQPYRDHIPIFTRNGVE AQLDRMLVPQVTLKSGGYLIINQTEALVAIDVNSGRSTREHSIEDTALQTNLEAAEEVARQLRLRDLAGLIVVDFIDMEE KRNNRAVEKKMKECLKDDRARIQVGRISHFGLLEMSRQRIRASVLESTMQVCPHCGGTGHIRSDSSMALHIIRGIEDYLL RHSGFDIHVRTPAASALYVLNHKRQILADLEGRFGVEISIDADESVGNQHFVIDKGAPSTRPVTPSAVQPMVYEDDIEDP EIPVEEDETEEEARTDVQAGEERSEENDRKRRRRRRRRGGRERDGRDLSAQEEAVSEEASEEDNETDQANEAVSSASMSE EDRRKKRRRGRRGGRKNRREDDNRARRIPDPEFVGYTPVLPVKAEESIGEVVETVETVEAPVVEAAPEEKPEKPARKTRT RKKAVDEAPADEAAAPAEEVVPEKPKRRTRKAAAKPVVEAEDVAEVKETVAAETQVETIAEPKSTEPVVTSSESEDRKPK RSGWWQKKGFF
Sequences:
>Translated_891_residues MSNKMLIDASHPEETRVIVTRGNKIEEFDFESEHKKQLKGNIYLARVTRVEPSLQAAFVEYGGNRHGFLAFSEIHPDYYQ IPVADRLALLEAEAKAARAEDDEDEPRAKSDRSERNDRARRNRRRGGRRDGQNGEAQANGADKDLPAAEPVGPGFADYVA GTPAAALTQKGDIISEALDNGDDDDVENEEDMVESVGSEDALEELPTHTRTHRRQYNIQEVIKRRQILLVQVVKEERGNK GAALTTYLSLAGRYSVLMPNTARGGGISRKITNPQDRKRLKEIVKELEVPQGMGVILRTAGANRTKAEVKRDYEYLMRLW ENVRTLTLQSTAPALVYEEGSLIKRSIRDIYNKDITEILVSGENGYREAKDFMRMLMPSHAKVVQPYRDHIPIFTRNGVE AQLDRMLVPQVTLKSGGYLIINQTEALVAIDVNSGRSTREHSIEDTALQTNLEAAEEVARQLRLRDLAGLIVVDFIDMEE KRNNRAVEKKMKECLKDDRARIQVGRISHFGLLEMSRQRIRASVLESTMQVCPHCGGTGHIRSDSSMALHIIRGIEDYLL RHSGFDIHVRTPAASALYVLNHKRQILADLEGRFGVEISIDADESVGNQHFVIDKGAPSTRPVTPSAVQPMVYEDDIEDP EIPVEEDETEEEARTDVQAGEERSEENDRKRRRRRRRRGGRERDGRDLSAQEEAVSEEASEEDNETDQANEAVSSASMSE EDRRKKRRRGRRGGRKNRREDDNRARRIPDPEFVGYTPVLPVKAEESIGEVVETVETVEAPVVEAAPEEKPEKPARKTRT RKKAVDEAPADEAAAPAEEVVPEKPKRRTRKAAAKPVVEAEDVAEVKETVAAETQVETIAEPKSTEPVVTSSESEDRKPK RSGWWQKKGFF >Mature_890_residues SNKMLIDASHPEETRVIVTRGNKIEEFDFESEHKKQLKGNIYLARVTRVEPSLQAAFVEYGGNRHGFLAFSEIHPDYYQI PVADRLALLEAEAKAARAEDDEDEPRAKSDRSERNDRARRNRRRGGRRDGQNGEAQANGADKDLPAAEPVGPGFADYVAG TPAAALTQKGDIISEALDNGDDDDVENEEDMVESVGSEDALEELPTHTRTHRRQYNIQEVIKRRQILLVQVVKEERGNKG AALTTYLSLAGRYSVLMPNTARGGGISRKITNPQDRKRLKEIVKELEVPQGMGVILRTAGANRTKAEVKRDYEYLMRLWE NVRTLTLQSTAPALVYEEGSLIKRSIRDIYNKDITEILVSGENGYREAKDFMRMLMPSHAKVVQPYRDHIPIFTRNGVEA QLDRMLVPQVTLKSGGYLIINQTEALVAIDVNSGRSTREHSIEDTALQTNLEAAEEVARQLRLRDLAGLIVVDFIDMEEK RNNRAVEKKMKECLKDDRARIQVGRISHFGLLEMSRQRIRASVLESTMQVCPHCGGTGHIRSDSSMALHIIRGIEDYLLR HSGFDIHVRTPAASALYVLNHKRQILADLEGRFGVEISIDADESVGNQHFVIDKGAPSTRPVTPSAVQPMVYEDDIEDPE IPVEEDETEEEARTDVQAGEERSEENDRKRRRRRRRRGGRERDGRDLSAQEEAVSEEASEEDNETDQANEAVSSASMSEE DRRKKRRRGRRGGRKNRREDDNRARRIPDPEFVGYTPVLPVKAEESIGEVVETVETVEAPVVEAAPEEKPEKPARKTRTR KKAVDEAPADEAAAPAEEVVPEKPKRRTRKAAAKPVVEAEDVAEVKETVAAETQVETIAEPKSTEPVVTSSESEDRKPKR SGWWQKKGFF
Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. It also cleaves RNA I, a molecule that controls the replication of colE1 plasmid DNA. It is the major endoribonuclease participating in mRNA turnover in E.coli. It initiates the decay of RNAs by
COG id: COG1530
COG function: function code J; Ribonucleases G and E
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 S1 motif domain [H]
Homologues:
Organism=Escherichia coli, GI1787325, Length=444, Percent_Identity=43.018018018018, Blast_Score=359, Evalue=1e-100, Organism=Escherichia coli, GI87082235, Length=339, Percent_Identity=37.4631268436578, Blast_Score=199, Evalue=5e-52,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012340 - InterPro: IPR016027 - InterPro: IPR021968 - InterPro: IPR003029 - InterPro: IPR019307 - InterPro: IPR022967 - InterPro: IPR004659 [H]
Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]
EC number: =3.1.26.12 [H]
Molecular weight: Translated: 100324; Mature: 100193
Theoretical pI: Translated: 5.50; Mature: 5.50
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.3 %Cys (Translated Protein) 1.9 %Met (Translated Protein) 2.2 %Cys+Met (Translated Protein) 0.3 %Cys (Mature Protein) 1.8 %Met (Mature Protein) 2.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSNKMLIDASHPEETRVIVTRGNKIEEFDFESEHKKQLKGNIYLARVTRVEPSLQAAFVE CCCCEEEECCCCCCCEEEEECCCCCCCCCCCHHHHHHHCCCEEEEEEEECCHHHHHHHHH YGGNRHGFLAFSEIHPDYYQIPVADRLALLEAEAKAARAEDDEDEPRAKSDRSERNDRAR HCCCCCCEEEECCCCCCCEECCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH RNRRRGGRRDGQNGEAQANGADKDLPAAEPVGPGFADYVAGTPAAALTQKGDIISEALDN HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHCCHHHHHHHCC GDDDDVENEEDMVESVGSEDALEELPTHTRTHRRQYNIQEVIKRRQILLVQVVKEERGNK CCCCCCCCHHHHHHHCCCHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC GAALTTYLSLAGRYSVLMPNTARGGGISRKITNPQDRKRLKEIVKELEVPQGMGVILRTA CCHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEC GANRTKAEVKRDYEYLMRLWENVRTLTLQSTAPALVYEEGSLIKRSIRDIYNKDITEILV CCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEECCCHHHHHHHHHHHCCCHHHHEE SGENGYREAKDFMRMLMPSHAKVVQPYRDHIPIFTRNGVEAQLDRMLVPQVTLKSGGYLI CCCCCHHHHHHHHHHHCCCHHHHHCCHHHCCCEEECCCCHHHHHHHHCCCEEECCCCEEE INQTEALVAIDVNSGRSTREHSIEDTALQTNLEAAEEVARQLRLRDLAGLIVVDFIDMEE EECCCEEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH KRNNRAVEKKMKECLKDDRARIQVGRISHFGLLEMSRQRIRASVLESTMQVCPHCGGTGH HHCCHHHHHHHHHHHHCCHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC IRSDSSMALHIIRGIEDYLLRHSGFDIHVRTPAASALYVLNHKRQILADLEGRFGVEISI CCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEE DADESVGNQHFVIDKGAPSTRPVTPSAVQPMVYEDDIEDPEIPVEEDETEEEARTDVQAG CCCCCCCCCEEEEECCCCCCCCCCHHHCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHCC EERSEENDRKRRRRRRRRGGRERDGRDLSAQEEAVSEEASEEDNETDQANEAVSSASMSE HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCH EDRRKKRRRGRRGGRKNRREDDNRARRIPDPEFVGYTPVLPVKAEESIGEVVETVETVEA HHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC PVVEAAPEEKPEKPARKTRTRKKAVDEAPADEAAAPAEEVVPEKPKRRTRKAAAKPVVEA CHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHCCCHHHHCCCCHHHHHHHHHHCCCCCH EDVAEVKETVAAETQVETIAEPKSTEPVVTSSESEDRKPKRSGWWQKKGFF HHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCC >Mature Secondary Structure SNKMLIDASHPEETRVIVTRGNKIEEFDFESEHKKQLKGNIYLARVTRVEPSLQAAFVE CCCEEEECCCCCCCEEEEECCCCCCCCCCCHHHHHHHCCCEEEEEEEECCHHHHHHHHH YGGNRHGFLAFSEIHPDYYQIPVADRLALLEAEAKAARAEDDEDEPRAKSDRSERNDRAR HCCCCCCEEEECCCCCCCEECCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH RNRRRGGRRDGQNGEAQANGADKDLPAAEPVGPGFADYVAGTPAAALTQKGDIISEALDN HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHCCHHHHHHHCC GDDDDVENEEDMVESVGSEDALEELPTHTRTHRRQYNIQEVIKRRQILLVQVVKEERGNK CCCCCCCCHHHHHHHCCCHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC GAALTTYLSLAGRYSVLMPNTARGGGISRKITNPQDRKRLKEIVKELEVPQGMGVILRTA CCHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEC GANRTKAEVKRDYEYLMRLWENVRTLTLQSTAPALVYEEGSLIKRSIRDIYNKDITEILV CCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEECCCHHHHHHHHHHHCCCHHHHEE SGENGYREAKDFMRMLMPSHAKVVQPYRDHIPIFTRNGVEAQLDRMLVPQVTLKSGGYLI CCCCCHHHHHHHHHHHCCCHHHHHCCHHHCCCEEECCCCHHHHHHHHCCCEEECCCCEEE INQTEALVAIDVNSGRSTREHSIEDTALQTNLEAAEEVARQLRLRDLAGLIVVDFIDMEE EECCCEEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH KRNNRAVEKKMKECLKDDRARIQVGRISHFGLLEMSRQRIRASVLESTMQVCPHCGGTGH HHCCHHHHHHHHHHHHCCHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC IRSDSSMALHIIRGIEDYLLRHSGFDIHVRTPAASALYVLNHKRQILADLEGRFGVEISI CCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEE DADESVGNQHFVIDKGAPSTRPVTPSAVQPMVYEDDIEDPEIPVEEDETEEEARTDVQAG CCCCCCCCCEEEEECCCCCCCCCCHHHCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHCC EERSEENDRKRRRRRRRRGGRERDGRDLSAQEEAVSEEASEEDNETDQANEAVSSASMSE HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCH EDRRKKRRRGRRGGRKNRREDDNRARRIPDPEFVGYTPVLPVKAEESIGEVVETVETVEA HHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC PVVEAAPEEKPEKPARKTRTRKKAVDEAPADEAAAPAEEVVPEKPKRRTRKAAAKPVVEA CHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHCCCHHHHCCCCHHHHHHHHHHCCCCCH EDVAEVKETVAAETQVETIAEPKSTEPVVTSSESEDRKPKRSGWWQKKGFF HHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8905232; 9278503; 1447789; 1704367; 2011493; 8415644 [H]