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The map label for this gene is mdeA [H]

Identifier: 225852307

GI number: 225852307

Start: 794304

End: 795587

Strand: Direct

Name: mdeA [H]

Synonym: BMEA_A0831

Alternate gene names: 225852307

Gene position: 794304-795587 (Clockwise)

Preceding gene: 225852301

Following gene: 225852309

Centisome position: 37.37

GC content: 57.24

Gene sequence:

>1284_bases
ATGTCAAAACGTTCGCCCGGAATTGAAACGCTTGCTGTTCACGCCGGCGCAAAGCCAGATCCGGCGACAGGTGCCCGTGC
AACGCCGATCTATCAAACCACATCCTTTGTGTTCGAGGATGCCGACCATGCGGCGGCCCTGTTCGGCCTTCAGGCCTTCG
GCAATATCTATACGCGCATCACCAATCCGACGACCGCCGTTCTCGAAGAGCGCATTGCAGCACTTGAAGGCGGAACGGCC
GCGGTCGCGACGGCTTCCGGCCATGCCGCGCAGTTTCTCGCCTTCCATACGTTGTTGCAGCCGGGTGATAATTTTATCGC
CGCGCGCCAGCTTTATGGCGGTTCGATCAATCAGTTTGGTCAGTCGTTCAAATCTTTCGACTGGCAGGTGCGCTGGGCCG
ATGCGACGAACCCGGCCGATTTTGCCAAACAGATCGATGAGCGCACACGCGCCATCTTCATCGAAAGCTTCGCCAATCCG
GGCGGCATCGTGGTGGATATCGAGGCCATTGCGGAAATCGCGCATCGCAATGGCCTGCCATTGATCGTGGACAATACGCT
TGCCTCACCCTATCTGGTGCGTCCGATCGAGCACGGTGCGGATATCGTGGTCCATTCGCTGACCAAATTTATCGGCGGGC
ACGGCAATTCCATGGGCGGCATTCTGGTCGATGGCGGCACGTTCGACTGGGCCAAATCCGGCAATTATCCGCTTCTGACC
GAACCGCGCCCGGATTATGCCGGTCTGGAACTGCACAAGACTTTTGGCAATATTTCCTTTGCCATTGCGGCCCGTGTGCT
GGGGCTTCGTGATTTCGGCCCTGCCATTTCGCCTTTCAATGCGTTCCAGATTCTCACCGGCGTTGAAACGCTTGCCCTGC
GTATGCAGCGCCATAGCGACAATGCGCTGAAGGTTGCCACATGGCTGCATGGCCATGAAAAAGTATCCTGGGTCAATTAT
GCCGGTCTGCCGCATGACAAATATCATGCCTTGCAGCAGAAATATGCGCCGAAGGGTGCGGGGTCCGTTTTCACTTTCGG
CATCAAGGGCGGTTATGAAGCGGGTGTGAAGTTCGTGGATAGTCTCGAACTTTTCTCGCTGCTTGCCAATCTGGGCGATA
CGCGTTCGCTGGTCATTCATCCGGCTTCAACGACGCACCGTCAGTTGAGTGACGAGCAGAAAGTGGCTGCAGGCGCCGGG
CCGGACGTGGTTCGCCTTTCCATCGGCATTGAAGATGCCGACGATATCATCGCCGATCTGGAGCAGGCGCTCGCAAAAGC
CTGA

Upstream 100 bases:

>100_bases
AAAATCCTCCTTTGCTTTCCTGGCGGCAACGCCTAGGTTCAGGCCGGATTTAGGCCCGGAGTGGGGCTCAAATCGGGGCT
TGGGGACACAGGAGGAAATA

Downstream 100 bases:

>100_bases
AGCGGCGAGCCGAATTTTATCAAGCAATGACGAGACATGCTGCGAAGCCCTTCGCAGCATGTCTTTATGAATCAGCGCGG
GCCGCGTTCGGGGGCGTGGA

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSKRSPGIETLAVHAGAKPDPATGARATPIYQTTSFVFEDADHAAALFGLQAFGNIYTRITNPTTAVLEERIAALEGGTA
AVATASGHAAQFLAFHTLLQPGDNFIAARQLYGGSINQFGQSFKSFDWQVRWADATNPADFAKQIDERTRAIFIESFANP
GGIVVDIEAIAEIAHRNGLPLIVDNTLASPYLVRPIEHGADIVVHSLTKFIGGHGNSMGGILVDGGTFDWAKSGNYPLLT
EPRPDYAGLELHKTFGNISFAIAARVLGLRDFGPAISPFNAFQILTGVETLALRMQRHSDNALKVATWLHGHEKVSWVNY
AGLPHDKYHALQQKYAPKGAGSVFTFGIKGGYEAGVKFVDSLELFSLLANLGDTRSLVIHPASTTHRQLSDEQKVAAGAG
PDVVRLSIGIEDADDIIADLEQALAKA

Sequences:

>Translated_427_residues
MSKRSPGIETLAVHAGAKPDPATGARATPIYQTTSFVFEDADHAAALFGLQAFGNIYTRITNPTTAVLEERIAALEGGTA
AVATASGHAAQFLAFHTLLQPGDNFIAARQLYGGSINQFGQSFKSFDWQVRWADATNPADFAKQIDERTRAIFIESFANP
GGIVVDIEAIAEIAHRNGLPLIVDNTLASPYLVRPIEHGADIVVHSLTKFIGGHGNSMGGILVDGGTFDWAKSGNYPLLT
EPRPDYAGLELHKTFGNISFAIAARVLGLRDFGPAISPFNAFQILTGVETLALRMQRHSDNALKVATWLHGHEKVSWVNY
AGLPHDKYHALQQKYAPKGAGSVFTFGIKGGYEAGVKFVDSLELFSLLANLGDTRSLVIHPASTTHRQLSDEQKVAAGAG
PDVVRLSIGIEDADDIIADLEQALAKA
>Mature_426_residues
SKRSPGIETLAVHAGAKPDPATGARATPIYQTTSFVFEDADHAAALFGLQAFGNIYTRITNPTTAVLEERIAALEGGTAA
VATASGHAAQFLAFHTLLQPGDNFIAARQLYGGSINQFGQSFKSFDWQVRWADATNPADFAKQIDERTRAIFIESFANPG
GIVVDIEAIAEIAHRNGLPLIVDNTLASPYLVRPIEHGADIVVHSLTKFIGGHGNSMGGILVDGGTFDWAKSGNYPLLTE
PRPDYAGLELHKTFGNISFAIAARVLGLRDFGPAISPFNAFQILTGVETLALRMQRHSDNALKVATWLHGHEKVSWVNYA
GLPHDKYHALQQKYAPKGAGSVFTFGIKGGYEAGVKFVDSLELFSLLANLGDTRSLVIHPASTTHRQLSDEQKVAAGAGP
DVVRLSIGIEDADDIIADLEQALAKA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=421, Percent_Identity=32.7790973871734, Blast_Score=172, Evalue=7e-43,
Organism=Homo sapiens, GI299473759, Length=421, Percent_Identity=29.4536817102138, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI25453487, Length=419, Percent_Identity=28.1622911694511, Blast_Score=113, Evalue=3e-25,
Organism=Escherichia coli, GI1790375, Length=418, Percent_Identity=30.8612440191388, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI1789383, Length=436, Percent_Identity=24.5412844036697, Blast_Score=124, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71980708, Length=416, Percent_Identity=31.25, Blast_Score=188, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17538055, Length=426, Percent_Identity=31.2206572769953, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17560056, Length=426, Percent_Identity=31.924882629108, Blast_Score=176, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6323333, Length=431, Percent_Identity=47.0997679814385, Blast_Score=356, Evalue=4e-99,
Organism=Saccharomyces cerevisiae, GI6319307, Length=433, Percent_Identity=32.3325635103926, Blast_Score=182, Evalue=7e-47,
Organism=Saccharomyces cerevisiae, GI6321254, Length=445, Percent_Identity=24.9438202247191, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321170, Length=373, Percent_Identity=26.8096514745308, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6321904, Length=429, Percent_Identity=25.1748251748252, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19922554, Length=425, Percent_Identity=33.8823529411765, Blast_Score=199, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45723; Mature: 45592

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRSPGIETLAVHAGAKPDPATGARATPIYQTTSFVFEDADHAAALFGLQAFGNIYTRI
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEHHHHHCCCCCHHHHHHHHHHHCCHHEEE
TNPTTAVLEERIAALEGGTAAVATASGHAAQFLAFHTLLQPGDNFIAARQLYGGSINQFG
CCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEHHHHCCCCHHHHH
QSFKSFDWQVRWADATNPADFAKQIDERTRAIFIESFANPGGIVVDIEAIAEIAHRNGLP
HHHHCCCCEEEECCCCCHHHHHHHHHHHHHEEEEECCCCCCCEEEEHHHHHHHHHCCCCC
LIVDNTLASPYLVRPIEHGADIVVHSLTKFIGGHGNSMGGILVDGGTFDWAKSGNYPLLT
EEEECCCCCCEEECCHHCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCEEC
EPRPDYAGLELHKTFGNISFAIAARVLGLRDFGPAISPFNAFQILTGVETLALRMQRHSD
CCCCCCCCCEEEHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
NALKVATWLHGHEKVSWVNYAGLPHDKYHALQQKYAPKGAGSVFTFGIKGGYEAGVKFVD
CEEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHH
SLELFSLLANLGDTRSLVIHPASTTHRQLSDEQKVAAGAGPDVVRLSIGIEDADDIIADL
HHHHHHHHHCCCCCCEEEEECCCCHHHHCCCHHHHHCCCCCCEEEEEECCCCHHHHHHHH
EQALAKA
HHHHHCC
>Mature Secondary Structure 
SKRSPGIETLAVHAGAKPDPATGARATPIYQTTSFVFEDADHAAALFGLQAFGNIYTRI
CCCCCCCEEEEEECCCCCCCCCCCCCCCCEEHHHHHCCCCCHHHHHHHHHHHCCHHEEE
TNPTTAVLEERIAALEGGTAAVATASGHAAQFLAFHTLLQPGDNFIAARQLYGGSINQFG
CCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEHHHHCCCCHHHHH
QSFKSFDWQVRWADATNPADFAKQIDERTRAIFIESFANPGGIVVDIEAIAEIAHRNGLP
HHHHCCCCEEEECCCCCHHHHHHHHHHHHHEEEEECCCCCCCEEEEHHHHHHHHHCCCCC
LIVDNTLASPYLVRPIEHGADIVVHSLTKFIGGHGNSMGGILVDGGTFDWAKSGNYPLLT
EEEECCCCCCEEECCHHCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCEEC
EPRPDYAGLELHKTFGNISFAIAARVLGLRDFGPAISPFNAFQILTGVETLALRMQRHSD
CCCCCCCCCEEEHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
NALKVATWLHGHEKVSWVNYAGLPHDKYHALQQKYAPKGAGSVFTFGIKGGYEAGVKFVD
CEEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHH
SLELFSLLANLGDTRSLVIHPASTTHRQLSDEQKVAAGAGPDVVRLSIGIEDADDIIADL
HHHHHHHHHCCCCCCEEEEECCCCHHHHCCCHHHHHCCCCCCEEEEEECCCCHHHHHHHH
EQALAKA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]