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The map label for this gene is ptsN [H]

Identifier: 225851694

GI number: 225851694

Start: 177625

End: 178089

Strand: Direct

Name: ptsN [H]

Synonym: BMEA_A0167

Alternate gene names: 225851694

Gene position: 177625-178089 (Clockwise)

Preceding gene: 225851693

Following gene: 225851699

Centisome position: 8.36

GC content: 58.49

Gene sequence:

>465_bases
ATGGATCTTAGCGATCTGATTCAGCCCGGGGCGATTATCCCGGCGCTGAAAGCCAGCTCCAAAAAGCAGCTTTTGCAGAT
TCTGTCTGAGAAGGCTGCGGAACTGACCGGTCTCCCTGAGCGTGAAGTTTTTGAAACAGTTCTCCAGCGTGAGCGCCTCG
GTTCCACCGGGGTGGGGAATGGCGTCGCCATTCCGCATGGCAAGCTGGCGAGCATCAACAAAATCGCCGGAATCTTTGCG
CGCCTTGAAACGCCGGTCGATTTTGAGGCGCTGGACGATCAGCCTGTCGATCTCGTTTTTCTGCTGTTGGCCCCGGAAAA
TGCCGGTGCCGATCATCTGAAAGCACTGTCGCGCATCGCCCGTTTGCTGCGCGACCCCGATATGGTGGCCAAATTGCGCG
GCACCCATGACGCGCAGGCCCTCTATTCATTCCTGACGGCTCACCCGACGTCCAACGCCGCCTGA

Upstream 100 bases:

>100_bases
GCAAACCAGAAGCGCACGGGAGCGTAAAGCCTGAAGGCGGCTTGCGTGCGCTTCCCAAAACCGGGCCGTAACCGGCCTGA
CTGAAGAAGGAACGGAGAGA

Downstream 100 bases:

>100_bases
GCCTGGTTGATTTCAATAACAAAGGCCGCCGTCCCCACGGGATGGCGGCCTTGTTTTTGAGGTGTTTCGCGCAATGGCTG
TCAATGCAGCGTCACCGTGC

Product: PTS IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Nitrogen-metabolic PTS system EIIA component [H]

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MDLSDLIQPGAIIPALKASSKKQLLQILSEKAAELTGLPEREVFETVLQRERLGSTGVGNGVAIPHGKLASINKIAGIFA
RLETPVDFEALDDQPVDLVFLLLAPENAGADHLKALSRIARLLRDPDMVAKLRGTHDAQALYSFLTAHPTSNAA

Sequences:

>Translated_154_residues
MDLSDLIQPGAIIPALKASSKKQLLQILSEKAAELTGLPEREVFETVLQRERLGSTGVGNGVAIPHGKLASINKIAGIFA
RLETPVDFEALDDQPVDLVFLLLAPENAGADHLKALSRIARLLRDPDMVAKLRGTHDAQALYSFLTAHPTSNAA
>Mature_154_residues
MDLSDLIQPGAIIPALKASSKKQLLQILSEKAAELTGLPEREVFETVLQRERLGSTGVGNGVAIPHGKLASINKIAGIFA
RLETPVDFEALDDQPVDLVFLLLAPENAGADHLKALSRIARLLRDPDMVAKLRGTHDAQALYSFLTAHPTSNAA

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=147, Percent_Identity=42.8571428571429, Blast_Score=126, Evalue=7e-31,
Organism=Escherichia coli, GI1786951, Length=136, Percent_Identity=33.8235294117647, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI1788726, Length=134, Percent_Identity=35.0746268656716, Blast_Score=69, Evalue=1e-13,
Organism=Escherichia coli, GI2367327, Length=93, Percent_Identity=31.1827956989247, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16497; Mature: 16497

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLSDLIQPGAIIPALKASSKKQLLQILSEKAAELTGLPEREVFETVLQRERLGSTGVGN
CCHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC
GVAIPHGKLASINKIAGIFARLETPVDFEALDDQPVDLVFLLLAPENAGADHLKALSRIA
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHH
RLLRDPDMVAKLRGTHDAQALYSFLTAHPTSNAA
HHHCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDLSDLIQPGAIIPALKASSKKQLLQILSEKAAELTGLPEREVFETVLQRERLGSTGVGN
CCHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC
GVAIPHGKLASINKIAGIFARLETPVDFEALDDQPVDLVFLLLAPENAGADHLKALSRIA
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHH
RLLRDPDMVAKLRGTHDAQALYSFLTAHPTSNAA
HHHCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275; 1991712 [H]