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The map label for this gene is dnaN [H]

Identifier: 225851548

GI number: 225851548

Start: 2503

End: 3696

Strand: Direct

Name: dnaN [H]

Synonym: BMEA_A0002

Alternate gene names: 225851548

Gene position: 2503-3696 (Clockwise)

Preceding gene: 225851547

Following gene: 225851549

Centisome position: 0.12

GC content: 58.79

Gene sequence:

>1194_bases
GTGCGCCTTATCGACAAGGGCCGTCTGCCCCTCGGATGCAGTCGCCCCGGTCCTAGCGAGCGAGTGCGTTTCAAGATGCG
TGTTACCCTAGAGCGATCGAACCTTCTTAAATCTCTCAACCACGTCCATCGCGTCGTGGAGCGCCGCAACACGATTCCAA
TTCTGTCGAATGTGCTTTTGCAGGCCGAAGGCGCAAGCCTCGCCATGAAGGCAACCGACCTGGATCTCGAAGTGAACGAG
GCGACCGCCGCCATGGTAGAGCAGGCGGGCGCCACCACGGTCCCGGCGCATCTTCTCTATGATATTGTTCGCAAGCTGCC
GGATGGCGCGGAAGTGATGCTGTCCACCAATCCCGATGGCGGCTCCATGTCGGTCATTTCGGGCAAGTCTTCTTTCCGCC
TGCAATGCCTGCCGCAATCCGATTTCCCGGAACTGACCGCTGGCGCCTTCACCCATTCCTTCCGCATCGAGGCGCAGGCT
CTCAAGCGCCTCATCGACCGCACGCAGTTTGCAATCTCGACGGAAGAAACCCGTTATTACCTGAACGGTATCTTCTTCCA
TGCGATTGAAAGCGATGGCGCGTTGAAGCTTCGGGCGGTTGCAACCGACGGGCACCGTCTGGCGCGCGCCGAACTGGAGG
CGCCCTCCGGCACGGAAGGCATGCCGGGCATCATCATCCCGCGCAAGACGGTTGCTGAATTGCAGAAGCTGGTCGATGTG
CCGGATGTGGTTGTGACCGTTGAGCTTTCCGATGCCAAGATCCGCTTCACCGTCGGCTCGGTGGTGCTCACCTCCAAGCT
TATCGACGGCACTTTCCCGGATTATCAGCGCGTCATTCCATCAGGCAACGACAAGAAGCTCACCATTGACCGGCAGGATT
TTGCTGCTTCCGTTGACCGCGTTTCGACGATTTCGAGTGAGCGTGGCCGCGCGGTGAAGCTTTCGATTGCCGATGGACAA
TTGACGCTCACCGTCAACAATCCCGATTCGGGCAGTGCGACCGATGAGCTTGCCGCCGATTATGATGGCGATCCGCTCGA
TATCGGCTTCAACTCCAAATATCTGCTTGATATCACCGGCCAGCTTTCCGGTACCGATGCGGTTTTCATGCTGGCCGATG
CCGGCTCTCCGACGCTGGTGCGCGATACGGGCGATGAAGATGTGCTCTATGTCCTCATGCCGATGCGCGTCTAA

Upstream 100 bases:

>100_bases
GAAACGGTTTTCGGATAAGATGCGCGTCAAAATAAATAGTTAGAGCATGGGTCTGGTGCATTCAGATCGAAACCTGCTCT
AACGGACCTTCTGGCGCCCT

Downstream 100 bases:

>100_bases
TGGGGCTGGGATATTTCGGTTCTGACGGCCTGCAAATGGCGCGGGTTTTTGCTTCCGGTGCTCGTGTAGCCCAAACTACA
TTCTGCTCCGGTTTTCAAAC

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MRLIDKGRLPLGCSRPGPSERVRFKMRVTLERSNLLKSLNHVHRVVERRNTIPILSNVLLQAEGASLAMKATDLDLEVNE
ATAAMVEQAGATTVPAHLLYDIVRKLPDGAEVMLSTNPDGGSMSVISGKSSFRLQCLPQSDFPELTAGAFTHSFRIEAQA
LKRLIDRTQFAISTEETRYYLNGIFFHAIESDGALKLRAVATDGHRLARAELEAPSGTEGMPGIIIPRKTVAELQKLVDV
PDVVVTVELSDAKIRFTVGSVVLTSKLIDGTFPDYQRVIPSGNDKKLTIDRQDFAASVDRVSTISSERGRAVKLSIADGQ
LTLTVNNPDSGSATDELAADYDGDPLDIGFNSKYLLDITGQLSGTDAVFMLADAGSPTLVRDTGDEDVLYVLMPMRV

Sequences:

>Translated_397_residues
MRLIDKGRLPLGCSRPGPSERVRFKMRVTLERSNLLKSLNHVHRVVERRNTIPILSNVLLQAEGASLAMKATDLDLEVNE
ATAAMVEQAGATTVPAHLLYDIVRKLPDGAEVMLSTNPDGGSMSVISGKSSFRLQCLPQSDFPELTAGAFTHSFRIEAQA
LKRLIDRTQFAISTEETRYYLNGIFFHAIESDGALKLRAVATDGHRLARAELEAPSGTEGMPGIIIPRKTVAELQKLVDV
PDVVVTVELSDAKIRFTVGSVVLTSKLIDGTFPDYQRVIPSGNDKKLTIDRQDFAASVDRVSTISSERGRAVKLSIADGQ
LTLTVNNPDSGSATDELAADYDGDPLDIGFNSKYLLDITGQLSGTDAVFMLADAGSPTLVRDTGDEDVLYVLMPMRV
>Mature_397_residues
MRLIDKGRLPLGCSRPGPSERVRFKMRVTLERSNLLKSLNHVHRVVERRNTIPILSNVLLQAEGASLAMKATDLDLEVNE
ATAAMVEQAGATTVPAHLLYDIVRKLPDGAEVMLSTNPDGGSMSVISGKSSFRLQCLPQSDFPELTAGAFTHSFRIEAQA
LKRLIDRTQFAISTEETRYYLNGIFFHAIESDGALKLRAVATDGHRLARAELEAPSGTEGMPGIIIPRKTVAELQKLVDV
PDVVVTVELSDAKIRFTVGSVVLTSKLIDGTFPDYQRVIPSGNDKKLTIDRQDFAASVDRVSTISSERGRAVKLSIADGQ
LTLTVNNPDSGSATDELAADYDGDPLDIGFNSKYLLDITGQLSGTDAVFMLADAGSPTLVRDTGDEDVLYVLMPMRV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=376, Percent_Identity=40.1595744680851, Blast_Score=264, Evalue=6e-72,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 43099; Mature: 43099

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00387 PPASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLIDKGRLPLGCSRPGPSERVRFKMRVTLERSNLLKSLNHVHRVVERRNTIPILSNVLL
CCCCCCCCCCCCCCCCCCCHHEEEEEEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHE
QAEGASLAMKATDLDLEVNEATAAMVEQAGATTVPAHLLYDIVRKLPDGAEVMLSTNPDG
EECCCEEEEEEECCCEEECHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCC
GSMSVISGKSSFRLQCLPQSDFPELTAGAFTHSFRIEAQALKRLIDRTQFAISTEETRYY
CEEEEEECCCCEEEEEECCCCCCCCHHHHEEEEEEEEHHHHHHHHHHHHEEEECCCCEEE
LNGIFFHAIESDGALKLRAVATDGHRLARAELEAPSGTEGMPGIIIPRKTVAELQKLVDV
EEEEEEEEECCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCEEEECCHHHHHHHHHHCC
PDVVVTVELSDAKIRFTVGSVVLTSKLIDGTFPDYQRVIPSGNDKKLTIDRQDFAASVDR
CCEEEEEEECCCEEEEEECCEEEEHHHHCCCCCCHHHHCCCCCCCEEEEEHHHHHHHHHH
VSTISSERGRAVKLSIADGQLTLTVNNPDSGSATDELAADYDGDPLDIGFNSKYLLDITG
HHHHHCCCCCEEEEEEECCEEEEEECCCCCCCCCHHHHCCCCCCEEEECCCCEEEEEEEC
QLSGTDAVFMLADAGSPTLVRDTGDEDVLYVLMPMRV
CCCCCCEEEEEECCCCCEEEECCCCCCEEEEEEECCC
>Mature Secondary Structure
MRLIDKGRLPLGCSRPGPSERVRFKMRVTLERSNLLKSLNHVHRVVERRNTIPILSNVLL
CCCCCCCCCCCCCCCCCCCHHEEEEEEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHE
QAEGASLAMKATDLDLEVNEATAAMVEQAGATTVPAHLLYDIVRKLPDGAEVMLSTNPDG
EECCCEEEEEEECCCEEECHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCC
GSMSVISGKSSFRLQCLPQSDFPELTAGAFTHSFRIEAQALKRLIDRTQFAISTEETRYY
CEEEEEECCCCEEEEEECCCCCCCCHHHHEEEEEEEEHHHHHHHHHHHHEEEECCCCEEE
LNGIFFHAIESDGALKLRAVATDGHRLARAELEAPSGTEGMPGIIIPRKTVAELQKLVDV
EEEEEEEEECCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCEEEECCHHHHHHHHHHCC
PDVVVTVELSDAKIRFTVGSVVLTSKLIDGTFPDYQRVIPSGNDKKLTIDRQDFAASVDR
CCEEEEEEECCCEEEEEECCEEEEHHHHCCCCCCHHHHCCCCCCCEEEEEHHHHHHHHHH
VSTISSERGRAVKLSIADGQLTLTVNNPDSGSATDELAADYDGDPLDIGFNSKYLLDITG
HHHHHCCCCCEEEEEEECCEEEEEECCCCCCCCCHHHHCCCCCCEEEECCCCEEEEEEEC
QLSGTDAVFMLADAGSPTLVRDTGDEDVLYVLMPMRV
CCCCCCEEEEEECCCCCEEEECCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]