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The map label for this gene is recF

Identifier: 225851549

GI number: 225851549

Start: 3878

End: 5032

Strand: Direct

Name: recF

Synonym: BMEA_A0003

Alternate gene names: 225851549

Gene position: 3878-5032 (Clockwise)

Preceding gene: 225851548

Following gene: 225851550

Centisome position: 0.18

GC content: 61.3

Gene sequence:

>1155_bases
GTGGAAGCAAAAGATCACATAGAACGCCGCCCGGATCGCGTTTCGATCCGGCGGCTCAAGCTTGTCAATTTCCGCAACTA
TGCAGAATTGTCGCTGCCGCTGGGACCGGGCCATGTGGTGCTGACGGGCGAAAACGGCTCCGGCAAAACCAATCTCATCG
AAGCCATTTCCTTCCTGTCGCCGGGGCGGGGCCTGCGTCGCGCCGCCTATGACGATGTGGCGCGTGCCAATGCCGAAGGC
GGTTTTGCGATCCACGCCGCACTCGACTGCATGATTTATGGCGACGCGGAAATCGGAACCGGCACGGCGGGCGGCGGCGA
AGGCGGGCGCAAGGTGCGTATCAACCGTATTGCCGCTTCTGCCGATGATCTTCTCGATTATGCGCGCATTCTCTGGGTGG
TGCCATCCATGGATGGGCTTTTCACCGGCGGCGCTTCCGACCGGCGGCGTTTTCTCGACCGTATGGTGCTCGCCATCGAT
ACCGCCCATGGCAAGCGGGTGCTTGATTATGAAAAAGCAATGCGCAGCCGCAACCGGCTGTTGAATGATGGCAGCAATGA
CGATCAATGGCTGGACGCCATCGAGAACCAGATGGCTGAACTGGGCACGGCCATTGCAGCAGCGCGTGCGCAAGCCATGC
GCCTGATTGCGGCGATGATCGAACGGCTTCCCGCCGAGGGACCATTTCCCAAAGCCGATTGCTTTCTGGAAGGGGCGCTG
GAAAGCCGCATCGGTGTGGAGGCGGCGCTTGATCTTGAAGAGGATTTTCGCCGTACCCTGCGCGATGGCCGCGCGCGCGA
CCGGGCGGCAGGCCGCACGCTCGATGGTCCGCATCGCACTGACCTGATTGTCCAGCACAGGCCCAAATCCATGCCGGCCG
CACTGTGCTCGACGGGCGAGCAGAAGGCGCTTCTGATCGGCCTCATTCTCGCCCATGCACGGCTGACGGCGGAACTTTCC
GGCATGGCGCCGATCCTCCTGCTCGATGAAATTGCGGCGCATCTCGATATGGGGCGCCGTGCAGCCCTTTTCGGCATTCT
CGACGAGCTTGGTGGGCAGGCTTTCATGACTGGCACCGATCGTGCGCTTTTTGATGCGCTTGCTGGCGATGCGCAGTTTT
TCAACGTGTCGGCCGGGCAATTGACAGGGATATAA

Upstream 100 bases:

>100_bases
TCTGCTCCGGTTTTCAAACCCCGCCATTTTTGGCTCGTCATCACCAAAATCTCGACAGCCTCCGGCGCCTCGCTGGAAAA
GCCCCGCAAAGAACCCGATT

Downstream 100 bases:

>100_bases
GGCAAAATAGTTGCTGACAGCGCGGTTCGGGCAGCCTATGTTTGCAGGCATGAATGTATCATCCAGACCATTTTCGCCCG
AAGAACTTGAACGCTATGCA

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MEAKDHIERRPDRVSIRRLKLVNFRNYAELSLPLGPGHVVLTGENGSGKTNLIEAISFLSPGRGLRRAAYDDVARANAEG
GFAIHAALDCMIYGDAEIGTGTAGGGEGGRKVRINRIAASADDLLDYARILWVVPSMDGLFTGGASDRRRFLDRMVLAID
TAHGKRVLDYEKAMRSRNRLLNDGSNDDQWLDAIENQMAELGTAIAAARAQAMRLIAAMIERLPAEGPFPKADCFLEGAL
ESRIGVEAALDLEEDFRRTLRDGRARDRAAGRTLDGPHRTDLIVQHRPKSMPAALCSTGEQKALLIGLILAHARLTAELS
GMAPILLLDEIAAHLDMGRRAALFGILDELGGQAFMTGTDRALFDALAGDAQFFNVSAGQLTGI

Sequences:

>Translated_384_residues
MEAKDHIERRPDRVSIRRLKLVNFRNYAELSLPLGPGHVVLTGENGSGKTNLIEAISFLSPGRGLRRAAYDDVARANAEG
GFAIHAALDCMIYGDAEIGTGTAGGGEGGRKVRINRIAASADDLLDYARILWVVPSMDGLFTGGASDRRRFLDRMVLAID
TAHGKRVLDYEKAMRSRNRLLNDGSNDDQWLDAIENQMAELGTAIAAARAQAMRLIAAMIERLPAEGPFPKADCFLEGAL
ESRIGVEAALDLEEDFRRTLRDGRARDRAAGRTLDGPHRTDLIVQHRPKSMPAALCSTGEQKALLIGLILAHARLTAELS
GMAPILLLDEIAAHLDMGRRAALFGILDELGGQAFMTGTDRALFDALAGDAQFFNVSAGQLTGI
>Mature_384_residues
MEAKDHIERRPDRVSIRRLKLVNFRNYAELSLPLGPGHVVLTGENGSGKTNLIEAISFLSPGRGLRRAAYDDVARANAEG
GFAIHAALDCMIYGDAEIGTGTAGGGEGGRKVRINRIAASADDLLDYARILWVVPSMDGLFTGGASDRRRFLDRMVLAID
TAHGKRVLDYEKAMRSRNRLLNDGSNDDQWLDAIENQMAELGTAIAAARAQAMRLIAAMIERLPAEGPFPKADCFLEGAL
ESRIGVEAALDLEEDFRRTLRDGRARDRAAGRTLDGPHRTDLIVQHRPKSMPAALCSTGEQKALLIGLILAHARLTAELS
GMAPILLLDEIAAHLDMGRRAALFGILDELGGQAFMTGTDRALFDALAGDAQFFNVSAGQLTGI

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=374, Percent_Identity=28.3422459893048, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_BRUA2 (Q2YPM3)

Other databases:

- EMBL:   AM040264
- RefSeq:   YP_413509.1
- ProteinModelPortal:   Q2YPM3
- STRING:   Q2YPM3
- GeneID:   3786823
- GenomeReviews:   AM040264_GR
- KEGG:   bmf:BAB1_0003
- eggNOG:   COG1195
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   PRK00064
- BioCyc:   BMEL359391:BAB1_0003-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- GO:   GO:0009295
- HAMAP:   MF_00365
- InterPro:   IPR003593
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- InterPro:   IPR007406
- Gene3D:   G3DSA:3.40.1140.10
- SMART:   SM00382
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 41495; Mature: 41495

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00617 RECF_1; PS00618 RECF_2; PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAKDHIERRPDRVSIRRLKLVNFRNYAELSLPLGPGHVVLTGENGSGKTNLIEAISFLS
CCCHHHHHCCCCHHHHHHHEECCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHC
PGRGLRRAAYDDVARANAEGGFAIHAALDCMIYGDAEIGTGTAGGGEGGRKVRINRIAAS
CCCCHHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEHHCC
ADDLLDYARILWVVPSMDGLFTGGASDRRRFLDRMVLAIDTAHGKRVLDYEKAMRSRNRL
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LNDGSNDDQWLDAIENQMAELGTAIAAARAQAMRLIAAMIERLPAEGPFPKADCFLEGAL
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
ESRIGVEAALDLEEDFRRTLRDGRARDRAAGRTLDGPHRTDLIVQHRPKSMPAALCSTGE
HHHCCCHHEECCHHHHHHHHHCCCHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHHCCCC
QKALLIGLILAHARLTAELSGMAPILLLDEIAAHLDMGRRAALFGILDELGGQAFMTGTD
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEECCCH
RALFDALAGDAQFFNVSAGQLTGI
HHHHHHHCCCCEEEECCCCCCCCC
>Mature Secondary Structure
MEAKDHIERRPDRVSIRRLKLVNFRNYAELSLPLGPGHVVLTGENGSGKTNLIEAISFLS
CCCHHHHHCCCCHHHHHHHEECCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHC
PGRGLRRAAYDDVARANAEGGFAIHAALDCMIYGDAEIGTGTAGGGEGGRKVRINRIAAS
CCCCHHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEHHCC
ADDLLDYARILWVVPSMDGLFTGGASDRRRFLDRMVLAIDTAHGKRVLDYEKAMRSRNRL
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LNDGSNDDQWLDAIENQMAELGTAIAAARAQAMRLIAAMIERLPAEGPFPKADCFLEGAL
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
ESRIGVEAALDLEEDFRRTLRDGRARDRAAGRTLDGPHRTDLIVQHRPKSMPAALCSTGE
HHHCCCHHEECCHHHHHHHHHCCCHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHHCCCC
QKALLIGLILAHARLTAELSGMAPILLLDEIAAHLDMGRRAALFGILDELGGQAFMTGTD
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEECCCH
RALFDALAGDAQFFNVSAGQLTGI
HHHHHHHCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA