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The map label for this gene is murF [H]

Identifier: 225630979

GI number: 225630979

Start: 1385838

End: 1387367

Strand: Direct

Name: murF [H]

Synonym: WRi_013010

Alternate gene names: 225630979

Gene position: 1385838-1387367 (Clockwise)

Preceding gene: 225630971

Following gene: 225630980

Centisome position: 95.85

GC content: 33.92

Gene sequence:

>1530_bases
ATGTTTAAATGGAACACAAATAATATCATTGATGCAACTGGCGGAAGAGACGTAAATGGTTGTAATTGGATACATAGCTC
AAATATTTCAACGGACACAAGAAGCATAAAAAAAGGTGATTTATTTATTGCCCTCAAGGGAAAGAATTTTGATGGCCATA
ATTTTTTGCATGAAGCGTTTTTAAAAGGGGCTGTCGCTGCAATAGTGAGTGAGGGTAAATATAAAAGTTTCCCTCTCATG
ATTGTACAAGATACCCTGAAAGCTTTGCACGATATGGCATCATACTACATTAGAAATGTTCTTATTGATGCTAAAGTTAT
TGCAGTTACAGGCAGTGTCGGGAAAACCACTACAAAGGATATGCTGCATACTGTTTTATCGCAATATGGAGTGTCCCATG
CAAGCGAAGGTAACTTAAATAATAATATAGGATTGCCTTTGACAATTCTGAAAGCTCCAAAAAACTGCCAGTACTTAATT
CTTGAAATGGGAATGAATAAAGTTGGTGAAATAAAAGAATTATCAAAAATTAGTAATCCGAATATTGCAGTCATTACCAA
CATCGAACCTGCACATACTGAAAATTTTTCATCACTATTTGACGTTGCACAAGCGAAGTTAGAAATTCTGTATGGTATGA
AAAATAACGGTACTTTGGTTTTAAGCAGAGATAATAAATATTATGATTATCTCTCATCACACGCCAATAGAAATGTAATA
AGCTTCGGTAAAGATAAAAATGCTGAAGTTTGTTTACTAAACTTAATAAGAAACGATGAATCTTCTGTCATTTCAGCACT
ATCTTCTGTCATCCCAGTGCTTGACTACTTGGATCCAGGAAGTTCAATTGCAAATGAGCATATCAGACGGTTGTATAATA
AAGACTGGATTCCAGCGTCACGCGCTGGAATGACATCAAACCGAGTTGCTAATAGATTAAGTTTAAAAATCAGGCTGAGT
GATAATCAAATTATAAACTGTAATTTACGTGTACAAGGTGAGCATTTTGCTTATTCGCTACTTGCTGTTGCAGCAGTTGT
GCAAAGCCTCGGACTTGATTTATCAAAATTACCACTTGCACTTAAGAATTTTAGTGTAGCGAAAGGTAGAGGCAATATTC
ATAAGGTCAAATGCAATAAAAAATATATACATTTAATTGATGACTCCTATAACGCCAGCCCTGCTTCGATGAAAACTGCA
ATAAAAACTTTGAGTACATATTCTAATCAGAGAAAAGTAGCTTTGCTTGGTGATATGTTGGAACTTGGTGATGAAAGCAT
AGAGTTTCATACAGATTTGGTTAAGATTATCACAGAAAATAATATAGATAAAGTTTATACAGTTGGTAAATTTATGTTAG
AATTGCATGAGCTTTTGCCAGATAATATAAGAGGCACGCATTTTAATGATTCTAATCAATTGAAAAGTGATTTAGCTAAC
ATTATTCAGAATAATGACGCAATTCTAGTTAAAGGCTCACGAGGAATGAAAATGGATCTTATTGTACGGGAATTCGTAAT
AAAATATTAA

Upstream 100 bases:

>100_bases
AGAATTATAGGGATTGCAACCATTATTTGCACATTGTCTAAAACATGTAGTATTTTCGCTACTTTTATTGTTCAATTTAT
TATAAAGAATACTCTAAATT

Downstream 100 bases:

>100_bases
GCGAAATCATTGTATTAATTTACAGTAACAAATTATTAGGAACTTTAATTGTGACAAAACGTGTAATTATTTTTGGTGGA
ACGGGGTTTATAGGGAAACA

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MFKWNTNNIIDATGGRDVNGCNWIHSSNISTDTRSIKKGDLFIALKGKNFDGHNFLHEAFLKGAVAAIVSEGKYKSFPLM
IVQDTLKALHDMASYYIRNVLIDAKVIAVTGSVGKTTTKDMLHTVLSQYGVSHASEGNLNNNIGLPLTILKAPKNCQYLI
LEMGMNKVGEIKELSKISNPNIAVITNIEPAHTENFSSLFDVAQAKLEILYGMKNNGTLVLSRDNKYYDYLSSHANRNVI
SFGKDKNAEVCLLNLIRNDESSVISALSSVIPVLDYLDPGSSIANEHIRRLYNKDWIPASRAGMTSNRVANRLSLKIRLS
DNQIINCNLRVQGEHFAYSLLAVAAVVQSLGLDLSKLPLALKNFSVAKGRGNIHKVKCNKKYIHLIDDSYNASPASMKTA
IKTLSTYSNQRKVALLGDMLELGDESIEFHTDLVKIITENNIDKVYTVGKFMLELHELLPDNIRGTHFNDSNQLKSDLAN
IIQNNDAILVKGSRGMKMDLIVREFVIKY

Sequences:

>Translated_509_residues
MFKWNTNNIIDATGGRDVNGCNWIHSSNISTDTRSIKKGDLFIALKGKNFDGHNFLHEAFLKGAVAAIVSEGKYKSFPLM
IVQDTLKALHDMASYYIRNVLIDAKVIAVTGSVGKTTTKDMLHTVLSQYGVSHASEGNLNNNIGLPLTILKAPKNCQYLI
LEMGMNKVGEIKELSKISNPNIAVITNIEPAHTENFSSLFDVAQAKLEILYGMKNNGTLVLSRDNKYYDYLSSHANRNVI
SFGKDKNAEVCLLNLIRNDESSVISALSSVIPVLDYLDPGSSIANEHIRRLYNKDWIPASRAGMTSNRVANRLSLKIRLS
DNQIINCNLRVQGEHFAYSLLAVAAVVQSLGLDLSKLPLALKNFSVAKGRGNIHKVKCNKKYIHLIDDSYNASPASMKTA
IKTLSTYSNQRKVALLGDMLELGDESIEFHTDLVKIITENNIDKVYTVGKFMLELHELLPDNIRGTHFNDSNQLKSDLAN
IIQNNDAILVKGSRGMKMDLIVREFVIKY
>Mature_509_residues
MFKWNTNNIIDATGGRDVNGCNWIHSSNISTDTRSIKKGDLFIALKGKNFDGHNFLHEAFLKGAVAAIVSEGKYKSFPLM
IVQDTLKALHDMASYYIRNVLIDAKVIAVTGSVGKTTTKDMLHTVLSQYGVSHASEGNLNNNIGLPLTILKAPKNCQYLI
LEMGMNKVGEIKELSKISNPNIAVITNIEPAHTENFSSLFDVAQAKLEILYGMKNNGTLVLSRDNKYYDYLSSHANRNVI
SFGKDKNAEVCLLNLIRNDESSVISALSSVIPVLDYLDPGSSIANEHIRRLYNKDWIPASRAGMTSNRVANRLSLKIRLS
DNQIINCNLRVQGEHFAYSLLAVAAVVQSLGLDLSKLPLALKNFSVAKGRGNIHKVKCNKKYIHLIDDSYNASPASMKTA
IKTLSTYSNQRKVALLGDMLELGDESIEFHTDLVKIITENNIDKVYTVGKFMLELHELLPDNIRGTHFNDSNQLKSDLAN
IIQNNDAILVKGSRGMKMDLIVREFVIKY

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=215, Percent_Identity=39.5348837209302, Blast_Score=150, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 56520; Mature: 56520

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKWNTNNIIDATGGRDVNGCNWIHSSNISTDTRSIKKGDLFIALKGKNFDGHNFLHEAF
CCEECCCCEEECCCCCCCCCCCCEECCCCCCCHHCCCCCCEEEEEECCCCCCHHHHHHHH
LKGAVAAIVSEGKYKSFPLMIVQDTLKALHDMASYYIRNVLIDAKVIAVTGSVGKTTTKD
HHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHH
MLHTVLSQYGVSHASEGNLNNNIGLPLTILKAPKNCQYLILEMGMNKVGEIKELSKISNP
HHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHCCCC
NIAVITNIEPAHTENFSSLFDVAQAKLEILYGMKNNGTLVLSRDNKYYDYLSSHANRNVI
CEEEEECCCCCCCCCHHHHHHHHHHHHHHEEEECCCCEEEEECCCCHHHHHHHCCCCCEE
SFGKDKNAEVCLLNLIRNDESSVISALSSVIPVLDYLDPGSSIANEHIRRLYNKDWIPAS
ECCCCCCCCEEEEEEHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCC
RAGMTSNRVANRLSLKIRLSDNQIINCNLRVQGEHFAYSLLAVAAVVQSLGLDLSKLPLA
CCCCCHHHHCCEEEEEEEECCCEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHCCHH
LKNFSVAKGRGNIHKVKCNKKYIHLIDDSYNASPASMKTAIKTLSTYSNQRKVALLGDML
HHCCEEECCCCCEEEEEECCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHH
ELGDESIEFHTDLVKIITENNIDKVYTVGKFMLELHELLPDNIRGTHFNDSNQLKSDLAN
HCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
IIQNNDAILVKGSRGMKMDLIVREFVIKY
HHCCCCEEEEECCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MFKWNTNNIIDATGGRDVNGCNWIHSSNISTDTRSIKKGDLFIALKGKNFDGHNFLHEAF
CCEECCCCEEECCCCCCCCCCCCEECCCCCCCHHCCCCCCEEEEEECCCCCCHHHHHHHH
LKGAVAAIVSEGKYKSFPLMIVQDTLKALHDMASYYIRNVLIDAKVIAVTGSVGKTTTKD
HHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHH
MLHTVLSQYGVSHASEGNLNNNIGLPLTILKAPKNCQYLILEMGMNKVGEIKELSKISNP
HHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHCCCC
NIAVITNIEPAHTENFSSLFDVAQAKLEILYGMKNNGTLVLSRDNKYYDYLSSHANRNVI
CEEEEECCCCCCCCCHHHHHHHHHHHHHHEEEECCCCEEEEECCCCHHHHHHHCCCCCEE
SFGKDKNAEVCLLNLIRNDESSVISALSSVIPVLDYLDPGSSIANEHIRRLYNKDWIPAS
ECCCCCCCCEEEEEEHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCC
RAGMTSNRVANRLSLKIRLSDNQIINCNLRVQGEHFAYSLLAVAAVVQSLGLDLSKLPLA
CCCCCHHHHCCEEEEEEEECCCEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHCCHH
LKNFSVAKGRGNIHKVKCNKKYIHLIDDSYNASPASMKTAIKTLSTYSNQRKVALLGDML
HHCCEEECCCCCEEEEEECCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHH
ELGDESIEFHTDLVKIITENNIDKVYTVGKFMLELHELLPDNIRGTHFNDSNQLKSDLAN
HCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
IIQNNDAILVKGSRGMKMDLIVREFVIKY
HHCCCCEEEEECCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]