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The map label for this gene is mdh

Identifier: 225630972

GI number: 225630972

Start: 1381834

End: 1382784

Strand: Reverse

Name: mdh

Synonym: WRi_012940

Alternate gene names: 225630972

Gene position: 1382784-1381834 (Counterclockwise)

Preceding gene: 225630973

Following gene: 225630968

Centisome position: 95.64

GC content: 37.64

Gene sequence:

>951_bases
ATGACAGTACAAAGAAAAAAAATATCTTTAATCGGTGCAGGAAATATCGGTGGAACTCTCACCCATATGATTGCACTAAG
GGAACTCGGTGATGTTGTTTTACTTGATATTAGTGATGGAATACCACAAGGTAAAGCACTCGACATAGCAGAATCATCCC
CTATTGATGGATTTAATGTCAATATTACTGGCACAAATAGGTATGAAGATATAAAAAACTCCGATGCAATTATAATAACC
GCTGGCATCGCTAGAAAACCTGGAATGAGCAGGGATGACCTCCTGCAAACCAATGCTAAAGTAATGAAGGAGGTTGGCGA
AAATATTAAAAAATATTCTCCAAACGCGTTTGTAATAGTGGTTACCAACCCTTTGGATGCCATGGTTTCAGTGGTGCATA
AATTTTCAAACCTTCCAACTAATATGATTGTTGGCATGGCAGGAGTGCTTGATTCTTCCCGTTTTCGTTACTTTCTTGCA
AGTGAGTTAAATATCTCAGTTGAAGATATATCTGCTTTTGTGCTTGGAGGACATGGCGACACTATGGTACCACTAATCAA
TTGTGCTTCAGTTGCTGGAGTTCCACTTACTCAAATTATCGATATGGGTCTTATCACACAGAAAAAAGTCGATGAAATAG
TTGAACGCACTCGCAATGGCGGTAAAGAAATAGTTGATCTGCTTAAGTCTGGATCTGCATACTACGCTCCTGCATCTTCA
GCTATATGCATGTTAGAATCATATTTAAAAGATAAGAGGCGCATATTACCATGTGCTGCTTACCTTAATGGTGAATATGG
TGTAGAAGAGTTGTTTATTGGTGTTCCTGTAATTATTGGAAAAAACGGAATTGAAAAGATCCTAGAAGTTAAAATGAATG
ATAGTGAACAAGAAATGTTTAATAAGTCTGTAAACTCGGTGAGAGAGCTGGTGAAATCTTTAGGTTCATAA

Upstream 100 bases:

>100_bases
TGTGTATAATCCTATAGATTTACCGCAAGATTTTCGTATGTTTGATAGCTTATCGCCTTGGGAAGGTAAAACCATAAAAG
TAAATACAAAGGAGTAGAAA

Downstream 100 bases:

>100_bases
GCAAATTAATGGTTTTATAGAGCTTCTTTATAGCTCAAACAGCCTAAAAAAGTTGCTTGTGGTTACCTTCGCTACTTGTT
CTGGTGACTCATTCCACAAC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MTVQRKKISLIGAGNIGGTLTHMIALRELGDVVLLDISDGIPQGKALDIAESSPIDGFNVNITGTNRYEDIKNSDAIIIT
AGIARKPGMSRDDLLQTNAKVMKEVGENIKKYSPNAFVIVVTNPLDAMVSVVHKFSNLPTNMIVGMAGVLDSSRFRYFLA
SELNISVEDISAFVLGGHGDTMVPLINCASVAGVPLTQIIDMGLITQKKVDEIVERTRNGGKEIVDLLKSGSAYYAPASS
AICMLESYLKDKRRILPCAAYLNGEYGVEELFIGVPVIIGKNGIEKILEVKMNDSEQEMFNKSVNSVRELVKSLGS

Sequences:

>Translated_316_residues
MTVQRKKISLIGAGNIGGTLTHMIALRELGDVVLLDISDGIPQGKALDIAESSPIDGFNVNITGTNRYEDIKNSDAIIIT
AGIARKPGMSRDDLLQTNAKVMKEVGENIKKYSPNAFVIVVTNPLDAMVSVVHKFSNLPTNMIVGMAGVLDSSRFRYFLA
SELNISVEDISAFVLGGHGDTMVPLINCASVAGVPLTQIIDMGLITQKKVDEIVERTRNGGKEIVDLLKSGSAYYAPASS
AICMLESYLKDKRRILPCAAYLNGEYGVEELFIGVPVIIGKNGIEKILEVKMNDSEQEMFNKSVNSVRELVKSLGS
>Mature_315_residues
TVQRKKISLIGAGNIGGTLTHMIALRELGDVVLLDISDGIPQGKALDIAESSPIDGFNVNITGTNRYEDIKNSDAIIITA
GIARKPGMSRDDLLQTNAKVMKEVGENIKKYSPNAFVIVVTNPLDAMVSVVHKFSNLPTNMIVGMAGVLDSSRFRYFLAS
ELNISVEDISAFVLGGHGDTMVPLINCASVAGVPLTQIIDMGLITQKKVDEIVERTRNGGKEIVDLLKSGSAYYAPASSA
ICMLESYLKDKRRILPCAAYLNGEYGVEELFIGVPVIIGKNGIEKILEVKMNDSEQEMFNKSVNSVRELVKSLGS

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI47059044, Length=316, Percent_Identity=34.4936708860759, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI221136809, Length=316, Percent_Identity=34.4936708860759, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI15082234, Length=320, Percent_Identity=33.125, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI9257228, Length=316, Percent_Identity=31.9620253164557, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI4504973, Length=316, Percent_Identity=31.9620253164557, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI5031857, Length=314, Percent_Identity=31.2101910828025, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI260099723, Length=314, Percent_Identity=31.2101910828025, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI291575128, Length=317, Percent_Identity=31.2302839116719, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI4557032, Length=317, Percent_Identity=31.2302839116719, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI260099727, Length=224, Percent_Identity=32.1428571428571, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI260099725, Length=196, Percent_Identity=33.6734693877551, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI207028494, Length=198, Percent_Identity=30.8080808080808, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI21735621, Length=319, Percent_Identity=26.0188087774295, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI103472011, Length=315, Percent_Identity=23.4920634920635, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI103472015, Length=179, Percent_Identity=26.2569832402235, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789632, Length=296, Percent_Identity=29.0540540540541, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17535107, Length=284, Percent_Identity=25.3521126760563, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17554310, Length=317, Percent_Identity=29.3375394321767, Blast_Score=102, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6322765, Length=298, Percent_Identity=30.2013422818792, Blast_Score=96, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6320125, Length=278, Percent_Identity=25.5395683453237, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324446, Length=175, Percent_Identity=30.2857142857143, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI17136226, Length=306, Percent_Identity=27.7777777777778, Blast_Score=140, Evalue=9e-34,
Organism=Drosophila melanogaster, GI45550422, Length=314, Percent_Identity=27.3885350318471, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24647881, Length=321, Percent_Identity=28.6604361370716, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24663599, Length=322, Percent_Identity=26.7080745341615, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24663595, Length=291, Percent_Identity=26.8041237113402, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_WOLPM (Q73G44)

Other databases:

- EMBL:   AE017196
- RefSeq:   NP_966840.1
- ProteinModelPortal:   Q73G44
- SMR:   Q73G44
- EnsemblBacteria:   EBWOLT00000003732
- GeneID:   2738185
- GenomeReviews:   AE017196_GR
- KEGG:   wol:WD1121
- TIGR:   WD_1121
- GeneTree:   EBGT00050000031315
- HOGENOM:   HBG566126
- OMA:   AMTYTVF
- PhylomeDB:   Q73G44
- ProtClustDB:   PRK06223
- BioCyc:   WPIP955:WD_1121-MONOMER
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01763

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 34138; Mature: 34007

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: ACT_SITE 178-178 BINDING 36-36 BINDING 85-85 BINDING 91-91 BINDING 98-98 BINDING 123-123 BINDING 154-154

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVQRKKISLIGAGNIGGTLTHMIALRELGDVVLLDISDGIPQGKALDIAESSPIDGFNV
CCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCEEECCCCCCCCCEEE
NITGTNRYEDIKNSDAIIITAGIARKPGMSRDDLLQTNAKVMKEVGENIKKYSPNAFVIV
EEECCCCHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
VTNPLDAMVSVVHKFSNLPTNMIVGMAGVLDSSRFRYFLASELNISVEDISAFVLGGHGD
EECCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHEEEEECCCCEEHHHEEEEEECCCCC
TMVPLINCASVAGVPLTQIIDMGLITQKKVDEIVERTRNGGKEIVDLLKSGSAYYAPASS
CHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEECCCHH
AICMLESYLKDKRRILPCAAYLNGEYGVEELFIGVPVIIGKNGIEKILEVKMNDSEQEMF
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEECCHHHHHHHHHHCCCCHHHHH
NKSVNSVRELVKSLGS
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TVQRKKISLIGAGNIGGTLTHMIALRELGDVVLLDISDGIPQGKALDIAESSPIDGFNV
CCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCEEECCCCCCCCCEEE
NITGTNRYEDIKNSDAIIITAGIARKPGMSRDDLLQTNAKVMKEVGENIKKYSPNAFVIV
EEECCCCHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
VTNPLDAMVSVVHKFSNLPTNMIVGMAGVLDSSRFRYFLASELNISVEDISAFVLGGHGD
EECCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHEEEEECCCCEEHHHEEEEEECCCCC
TMVPLINCASVAGVPLTQIIDMGLITQKKVDEIVERTRNGGKEIVDLLKSGSAYYAPASS
CHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEECCCHH
AICMLESYLKDKRRILPCAAYLNGEYGVEELFIGVPVIIGKNGIEKILEVKMNDSEQEMF
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEECCHHHHHHHHHHCCCCHHHHH
NKSVNSVRELVKSLGS
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA