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The map label for this gene is ppa [H]

Identifier: 225630919

GI number: 225630919

Start: 1328795

End: 1329310

Strand: Direct

Name: ppa [H]

Synonym: WRi_012350

Alternate gene names: 225630919

Gene position: 1328795-1329310 (Clockwise)

Preceding gene: 225630918

Following gene: 225630920

Centisome position: 91.9

GC content: 36.05

Gene sequence:

>516_bases
ATGGATTTAAGTAAAATAACAGCGGGACCAAATGCGGTGAATGTGGTAATTGAAATAAGTGCGAATGCTGAGCCTGTAAA
GTATGAATTTAATAAAGAGTTTGGGTTGTTACAAGTTGACAGATTTTTGTCTACCTCAATGACTTATCCTTGCAATTATG
GGTTTATACCAAATACCTGCGCAGGTGATGGTGATCCTGTGGATGTTTTGGTGCTAACTCAATTTCCCTTAGCATCTAGT
GTTTTAATATCGGTGCGTCCAATAGGCGCATTACTCACTAAAGATGAAAAAGGAGAAGATGAGAAAATATTAGCTGTGCC
TGTTTCCAGTGTTGATAGCTATTATGACAATATAAAGGACTATTCTGACTTATCTAAAAGCCTACTAGATAAAATTACTC
ATTTCTTTTCTCATTATAAAGATCTAGAAAAGGGAAAAACGGTAGCAGTTGGAGAATGGGTTGGTGTAGAAGAAGCAAAA
CAAATTGTTGCAAGCTCTATTAAAAGCGGAAATTAG

Upstream 100 bases:

>100_bases
TTTAGGACCGCTCCATGCAAAACAAGCTTAGAACTACATAAATGTAGCAACTTAAAAGTACATATTGTATAATGCTTATT
CTTAAGTTAAGAGGTAATTT

Downstream 100 bases:

>100_bases
TTTTTGTTGATTTATAGCTAAAGTGGCTCTACGATTTGAAAAATAACAAATTCGTCATTCCGCTACGTGTTAGCGGAATC
TATACCGCGCACAACTGCAC

Product: inorganic pyrophosphatase

Products: NA

Alternate protein names: Pyrophosphate phospho-hydrolase; PPase [H]

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MDLSKITAGPNAVNVVIEISANAEPVKYEFNKEFGLLQVDRFLSTSMTYPCNYGFIPNTCAGDGDPVDVLVLTQFPLASS
VLISVRPIGALLTKDEKGEDEKILAVPVSSVDSYYDNIKDYSDLSKSLLDKITHFFSHYKDLEKGKTVAVGEWVGVEEAK
QIVASSIKSGN

Sequences:

>Translated_171_residues
MDLSKITAGPNAVNVVIEISANAEPVKYEFNKEFGLLQVDRFLSTSMTYPCNYGFIPNTCAGDGDPVDVLVLTQFPLASS
VLISVRPIGALLTKDEKGEDEKILAVPVSSVDSYYDNIKDYSDLSKSLLDKITHFFSHYKDLEKGKTVAVGEWVGVEEAK
QIVASSIKSGN
>Mature_171_residues
MDLSKITAGPNAVNVVIEISANAEPVKYEFNKEFGLLQVDRFLSTSMTYPCNYGFIPNTCAGDGDPVDVLVLTQFPLASS
VLISVRPIGALLTKDEKGEDEKILAVPVSSVDSYYDNIKDYSDLSKSLLDKITHFFSHYKDLEKGKTVAVGEWVGVEEAK
QIVASSIKSGN

Specific function: Unknown

COG id: COG0221

COG function: function code C; Inorganic pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790673, Length=175, Percent_Identity=54.2857142857143, Blast_Score=179, Evalue=8e-47,

Paralogues:

None

Copy number: 5480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008162 [H]

Pfam domain/function: PF00719 Pyrophosphatase [H]

EC number: =3.6.1.1 [H]

Molecular weight: Translated: 18642; Mature: 18642

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00387 PPASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLSKITAGPNAVNVVIEISANAEPVKYEFNKEFGLLQVDRFLSTSMTYPCNYGFIPNTC
CCCCCCCCCCCEEEEEEEECCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
AGDGDPVDVLVLTQFPLASSVLISVRPIGALLTKDEKGEDEKILAVPVSSVDSYYDNIKD
CCCCCCEEEEEEECCCCCCCEEEEECCCHHEEECCCCCCCCEEEEEEHHHHHHHHHHHHH
YSDLSKSLLDKITHFFSHYKDLEKGKTVAVGEWVGVEEAKQIVASSIKSGN
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDLSKITAGPNAVNVVIEISANAEPVKYEFNKEFGLLQVDRFLSTSMTYPCNYGFIPNTC
CCCCCCCCCCCEEEEEEEECCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
AGDGDPVDVLVLTQFPLASSVLISVRPIGALLTKDEKGEDEKILAVPVSSVDSYYDNIKD
CCCCCCEEEEEEECCCCCCCEEEEECCCHHEEECCCCCCCCEEEEEEHHHHHHHHHHHHH
YSDLSKSLLDKITHFFSHYKDLEKGKTVAVGEWVGVEEAKQIVASSIKSGN
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]