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The map label for this gene is dapF

Identifier: 225630874

GI number: 225630874

Start: 1277520

End: 1278308

Strand: Direct

Name: dapF

Synonym: WRi_011860

Alternate gene names: 225630874

Gene position: 1277520-1278308 (Clockwise)

Preceding gene: 225630873

Following gene: 225630881

Centisome position: 88.36

GC content: 36.5

Gene sequence:

>789_bases
ATGAGTGGCCAAAATTCTGAAAATTTTATCAAAATGCACGGAACTGGTAACAACTTCGTTATCATAGACTCATGTTCAGC
AAATGATTTAAATTGGAACTATAAAAAAATTGCTAACCAAAACGGTTGCGATCAAGTCATAGTGATAACAAACTCCAGTT
TAGCCGATTGCTTTATGCATATCTATAATGCTGATGGTAGCAAAGCTGAAATGTGCGGAAATGCAGCACGTTGCGTTGGA
TATTTGCTAATGTCAGAAAAAGGTACTGAGTATATCACTATTGAGCTGGTAAACAAACGCATTTTAGAGTGTTTTAAAGT
GGGTGATAAATCCATAAAGGTTAACATGGGTAAACCTTTGCTAAAATGGCATAAAATTCCCTTGTCTACTGAGTGTGACA
CTCTTCACTTACCTATAGAGCTTGAAATGCTAAAAGATCCTGTTGCAGTAAATATTGGTAATCCTCACATAGTTTTTTTT
GTTGATAGTGTAAATGAAATACCATTGCAAAGTTTAGGACCAAAGCTAGAAAATCACGCATTATTTCCTCAGAAAATTAA
TATTAGTATTGCACAAGTTGAAAAGTCTGGAGAAATAGCTTTAAGAGTTTGGGAAAGAGGTACAGGCATTACTGCATCAT
GCGGTAGTGCAGCCTGTGCAGCACTTGTTACATCCACACTACGTGGATATCTGACTGTCCAACAGACTTCAGTAAATTTA
CCAGGAGGGAAGTTATTAATCGAATGGGCAAATAACGTATTCATGACCGGTGATATAGGGTTTTTGTGA

Upstream 100 bases:

>100_bases
GACTTTAACGTAATGTCAGTGATAGCGGTTGGTTACGAAGAAGAGGGTGCTGAAACAAGTAAGAAGAAAAGAAGACCAAT
AGAAGAAATATTTTTTTATG

Downstream 100 bases:

>100_bases
CCCTAATTCTAGTGATGCATTACTTCCAATACCTTCTTACCAATTACTGCAGGGGTTTCAGCAATTGCAATCCCGGCACT
CCGCATAACTTCTAGTTTTG

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MSGQNSENFIKMHGTGNNFVIIDSCSANDLNWNYKKIANQNGCDQVIVITNSSLADCFMHIYNADGSKAEMCGNAARCVG
YLLMSEKGTEYITIELVNKRILECFKVGDKSIKVNMGKPLLKWHKIPLSTECDTLHLPIELEMLKDPVAVNIGNPHIVFF
VDSVNEIPLQSLGPKLENHALFPQKINISIAQVEKSGEIALRVWERGTGITASCGSAACAALVTSTLRGYLTVQQTSVNL
PGGKLLIEWANNVFMTGDIGFL

Sequences:

>Translated_262_residues
MSGQNSENFIKMHGTGNNFVIIDSCSANDLNWNYKKIANQNGCDQVIVITNSSLADCFMHIYNADGSKAEMCGNAARCVG
YLLMSEKGTEYITIELVNKRILECFKVGDKSIKVNMGKPLLKWHKIPLSTECDTLHLPIELEMLKDPVAVNIGNPHIVFF
VDSVNEIPLQSLGPKLENHALFPQKINISIAQVEKSGEIALRVWERGTGITASCGSAACAALVTSTLRGYLTVQQTSVNL
PGGKLLIEWANNVFMTGDIGFL
>Mature_261_residues
SGQNSENFIKMHGTGNNFVIIDSCSANDLNWNYKKIANQNGCDQVIVITNSSLADCFMHIYNADGSKAEMCGNAARCVGY
LLMSEKGTEYITIELVNKRILECFKVGDKSIKVNMGKPLLKWHKIPLSTECDTLHLPIELEMLKDPVAVNIGNPHIVFFV
DSVNEIPLQSLGPKLENHALFPQKINISIAQVEKSGEIALRVWERGTGITASCGSAACAALVTSTLRGYLTVQQTSVNLP
GGKLLIEWANNVFMTGDIGFL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=263, Percent_Identity=34.6007604562738, Blast_Score=150, Evalue=9e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_WOLWR (C0R4N2)

Other databases:

- EMBL:   CP001391
- RefSeq:   YP_002727665.1
- ProteinModelPortal:   C0R4N2
- EnsemblBacteria:   EBWOLT00000002279
- GeneID:   7669898
- GenomeReviews:   CP001391_GR
- KEGG:   wri:WRi_011860
- GeneTree:   EBGT00050000031192
- OMA:   HTGIGFD
- ProtClustDB:   CLSK749319
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 28665; Mature: 28534

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 72-72 ACT_SITE 214-214

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGQNSENFIKMHGTGNNFVIIDSCSANDLNWNYKKIANQNGCDQVIVITNSSLADCFMH
CCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCHHHHCCCCCCCEEEEEECCCHHHHHHH
IYNADGSKAEMCGNAARCVGYLLMSEKGTEYITIELVNKRILECFKVGDKSIKVNMGKPL
HHCCCCCHHHHCCCHHHHHEEEEECCCCCEEEEEEEHHHHHHHHHHCCCCEEEEECCCCC
LKWHKIPLSTECDTLHLPIELEMLKDPVAVNIGNPHIVFFVDSVNEIPLQSLGPKLENHA
HHEECCCCCCCCCEEECCEEEEECCCCEEEECCCCEEEEEECCCCCCCHHHCCCCCCCCC
LFPQKINISIAQVEKSGEIALRVWERGTGITASCGSAACAALVTSTLRGYLTVQQTSVNL
CCCCEECEEEEEEECCCCEEEEEEECCCCCEECCCHHHHHHHHHHHHHEEEEEEEEEEEC
PGGKLLIEWANNVFMTGDIGFL
CCCEEEEEECCCEEEECCCCCC
>Mature Secondary Structure 
SGQNSENFIKMHGTGNNFVIIDSCSANDLNWNYKKIANQNGCDQVIVITNSSLADCFMH
CCCCCCCEEEEECCCCCEEEEECCCCCCCCCCHHHHCCCCCCCEEEEEECCCHHHHHHH
IYNADGSKAEMCGNAARCVGYLLMSEKGTEYITIELVNKRILECFKVGDKSIKVNMGKPL
HHCCCCCHHHHCCCHHHHHEEEEECCCCCEEEEEEEHHHHHHHHHHCCCCEEEEECCCCC
LKWHKIPLSTECDTLHLPIELEMLKDPVAVNIGNPHIVFFVDSVNEIPLQSLGPKLENHA
HHEECCCCCCCCCEEECCEEEEECCCCEEEECCCCEEEEEECCCCCCCHHHCCCCCCCCC
LFPQKINISIAQVEKSGEIALRVWERGTGITASCGSAACAALVTSTLRGYLTVQQTSVNL
CCCCEECEEEEEEECCCCEEEEEEECCCCCEECCCHHHHHHHHHHHHHEEEEEEEEEEEC
PGGKLLIEWANNVFMTGDIGFL
CCCEEEEEECCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA