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The map label for this gene is yaeL [C]

Identifier: 225630816

GI number: 225630816

Start: 1213247

End: 1214365

Strand: Reverse

Name: yaeL [C]

Synonym: WRi_011200

Alternate gene names: 225630816

Gene position: 1214365-1213247 (Counterclockwise)

Preceding gene: 225630818

Following gene: 225630815

Centisome position: 83.99

GC content: 34.32

Gene sequence:

>1119_bases
GTGGAATTAATTTCTAATCTTATCCATCAATTTAGCGATGGAATATATTATTTTTTATCATTTTCTTTAATAATTTCTGT
CATAGTATTCGTGCATGAATATGGGCATTATGTTGTTGCCAAAGCATGCAAAGTTAAAGTTGAATCTTTTTCCATAGGTT
TTGGTCCTGAAATTTTTGGTTTTAACGATAAGTCTGGAACTAGATGGAAATTAAGCGCTGTTCCATTGGGTGGTTATGTT
AAAATGCTAGGAGATACTAATGCAGCGAGTGTCCCAGCTGATCAACAAGAATTAACGGAAGAAGAAAAATTGTATTCATT
TCATACAAAACCGCGTCATAAAAAAGCAGCAGTAGTTTTTGCAGGGCCTTTTGCAAACATGGTATTTGCTGTTATAGCTT
TTACAATATTTTTTAGCATAGCAGGTTATTATCGCACTCCACCGGTGATTGAAAATGTAATCGAAGGTAGTGCAGCTAAG
CAAGCTGGTTTGTTGCCAGGTGACACTATTACACAAATCAATGAGCATAAAATAAAATACTTTGAAGATATTTCACGTGT
GATAATGTCGAATCCTAAAACAAGAATGGAGATTGAGTATAGCAGAAATAATGAGAAGCACAGGACCAGTTTAACTCCAT
TAATAATCAAGGACAAAGACGTTTTTGGCAACACAATAGAAAGAGAAACTATAGGGATTATTTCAGTCAATACATTAAAG
CAGTCGTCTTTTCTTGGGGCTGTGAGCTTATCAGTAAGTGAAACTTACCACACTATGTGCTTAACGATCAAAGCTCTCTT
TCACATTATTGTTGGTAAAAGGAGTATCAATGAAATAGGTGGACCAATAAAAATCGCAAAATATTCAGGACAATCAGCTA
AAAAGGGATTTATTATGGTTCTATATTTTATGGCAATCATTTCAGCTAATTTAGCTGCAATTAACTTGCTGCCAATACCA
CTGCTAGATGGTGGGCATTTATTTCATTATATCATAGAAGCGGTTATACGTAGGGATTTAAGCTTGAAATGTCAAAAATA
TGCAGCTATTTTTGGTGCTTTAGTGTTGTTTTTGCTTATGGCAACTGGAATGTCAAATGATATTAGGGATCTTTTTTGA

Upstream 100 bases:

>100_bases
TTACAATTAAACTTTTTTTAATTTAAAAGTTAAACTAAATACTTGTCAAGCGAGGCGCATTTTAATATACTTAACATTTT
GTTAAGATCTAAATATACAG

Downstream 100 bases:

>100_bases
AATAAAATATGAAAAAGCTATTTTATATACTGGTAGTGGTTTTTATTTCTTTTCCTGCCCTGCTTGTTGCTTTAGGAACT
GCGGAAAAAGTGCAGGTGAA

Product: membrane-associated zinc metalloprotease, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MELISNLIHQFSDGIYYFLSFSLIISVIVFVHEYGHYVVAKACKVKVESFSIGFGPEIFGFNDKSGTRWKLSAVPLGGYV
KMLGDTNAASVPADQQELTEEEKLYSFHTKPRHKKAAVVFAGPFANMVFAVIAFTIFFSIAGYYRTPPVIENVIEGSAAK
QAGLLPGDTITQINEHKIKYFEDISRVIMSNPKTRMEIEYSRNNEKHRTSLTPLIIKDKDVFGNTIERETIGIISVNTLK
QSSFLGAVSLSVSETYHTMCLTIKALFHIIVGKRSINEIGGPIKIAKYSGQSAKKGFIMVLYFMAIISANLAAINLLPIP
LLDGGHLFHYIIEAVIRRDLSLKCQKYAAIFGALVLFLLMATGMSNDIRDLF

Sequences:

>Translated_372_residues
MELISNLIHQFSDGIYYFLSFSLIISVIVFVHEYGHYVVAKACKVKVESFSIGFGPEIFGFNDKSGTRWKLSAVPLGGYV
KMLGDTNAASVPADQQELTEEEKLYSFHTKPRHKKAAVVFAGPFANMVFAVIAFTIFFSIAGYYRTPPVIENVIEGSAAK
QAGLLPGDTITQINEHKIKYFEDISRVIMSNPKTRMEIEYSRNNEKHRTSLTPLIIKDKDVFGNTIERETIGIISVNTLK
QSSFLGAVSLSVSETYHTMCLTIKALFHIIVGKRSINEIGGPIKIAKYSGQSAKKGFIMVLYFMAIISANLAAINLLPIP
LLDGGHLFHYIIEAVIRRDLSLKCQKYAAIFGALVLFLLMATGMSNDIRDLF
>Mature_372_residues
MELISNLIHQFSDGIYYFLSFSLIISVIVFVHEYGHYVVAKACKVKVESFSIGFGPEIFGFNDKSGTRWKLSAVPLGGYV
KMLGDTNAASVPADQQELTEEEKLYSFHTKPRHKKAAVVFAGPFANMVFAVIAFTIFFSIAGYYRTPPVIENVIEGSAAK
QAGLLPGDTITQINEHKIKYFEDISRVIMSNPKTRMEIEYSRNNEKHRTSLTPLIIKDKDVFGNTIERETIGIISVNTLK
QSSFLGAVSLSVSETYHTMCLTIKALFHIIVGKRSINEIGGPIKIAKYSGQSAKKGFIMVLYFMAIISANLAAINLLPIP
LLDGGHLFHYIIEAVIRRDLSLKCQKYAAIFGALVLFLLMATGMSNDIRDLF

Specific function: Unknown

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PDZ (DHR) domain [H]

Homologues:

Organism=Escherichia coli, GI1786373, Length=160, Percent_Identity=36.25, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR004387
- InterPro:   IPR008915 [H]

Pfam domain/function: PF00595 PDZ; PF02163 Peptidase_M50 [H]

EC number: NA

Molecular weight: Translated: 41373; Mature: 41373

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELISNLIHQFSDGIYYFLSFSLIISVIVFVHEYGHYVVAKACKVKVESFSIGFGPEIFG
CHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHEEEEEEECCCCCEEEC
FNDKSGTRWKLSAVPLGGYVKMLGDTNAASVPADQQELTEEEKLYSFHTKPRHKKAAVVF
CCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEE
AGPFANMVFAVIAFTIFFSIAGYYRTPPVIENVIEGSAAKQAGLLPGDTITQINEHKIKY
ECHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHH
FEDISRVIMSNPKTRMEIEYSRNNEKHRTSLTPLIIKDKDVFGNTIERETIGIISVNTLK
HHHHHHHHHCCCCCEEEEEECCCCCCHHHCCCEEEEECCHHCCCCCCCCEEEEEEEECHH
QSSFLGAVSLSVSETYHTMCLTIKALFHIIVGKRSINEIGGPIKIAKYSGQSAKKGFIMV
HCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEEEECCCCCCCCHHHH
LYFMAIISANLAAINLLPIPLLDGGHLFHYIIEAVIRRDLSLKCQKYAAIFGALVLFLLM
HHHHHHHHCCHHEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
ATGMSNDIRDLF
HCCCCCHHHHCC
>Mature Secondary Structure
MELISNLIHQFSDGIYYFLSFSLIISVIVFVHEYGHYVVAKACKVKVESFSIGFGPEIFG
CHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHEEEEEEECCCCCEEEC
FNDKSGTRWKLSAVPLGGYVKMLGDTNAASVPADQQELTEEEKLYSFHTKPRHKKAAVVF
CCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEE
AGPFANMVFAVIAFTIFFSIAGYYRTPPVIENVIEGSAAKQAGLLPGDTITQINEHKIKY
ECHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHH
FEDISRVIMSNPKTRMEIEYSRNNEKHRTSLTPLIIKDKDVFGNTIERETIGIISVNTLK
HHHHHHHHHCCCCCEEEEEECCCCCCHHHCCCEEEEECCHHCCCCCCCCEEEEEEEECHH
QSSFLGAVSLSVSETYHTMCLTIKALFHIIVGKRSINEIGGPIKIAKYSGQSAKKGFIMV
HCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEEEECCCCCCCCHHHH
LYFMAIISANLAAINLLPIPLLDGGHLFHYIIEAVIRRDLSLKCQKYAAIFGALVLFLLM
HHHHHHHHCCHHEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
ATGMSNDIRDLF
HCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]