Click here to switch to the map view.

The map label for this gene is xth [H]

Identifier: 225630665

GI number: 225630665

Start: 1021399

End: 1022178

Strand: Direct

Name: xth [H]

Synonym: WRi_009500

Alternate gene names: 225630665

Gene position: 1021399-1022178 (Clockwise)

Preceding gene: 225630664

Following gene: 225630673

Centisome position: 70.64

GC content: 34.49

Gene sequence:

>780_bases
ATGCTAAAGATTGCAACTTGGAACGTAAACTCCATACGTAAAAGAGTTAATCAACTTTGTAGTTTTATAGTTGATAGTCA
GATAGATATAGTTTTGCTGCAAGAGATAAAATGTACGGAAGAGCAATTTCCTTATGCAGAGATAGAAAAGTTAGGATATG
AATATGCTATCTATGGACAAGTTGCAAGAAATGGCGTTTGTGTTTTATCTAAATATCCAATACTGGAAAAGTTTAAAATT
GACATTGTAGAAGGTTATCAAGAAGCGCGTTATATAGAGTGCATAATAAAGCACAATAATCAGAAGGTAAGGGTGGGAAG
TGTATACGTTCCAAACGGTCAAAGCCCGGACTCTCAAGCATTTGAATATAAACTCAAGTTTTTTGATAATCTATATGAAA
GGATGAGCACTTTGTTAAAGAATGAAGAGTTAACTATTATAGCTGGCGATTATAATGTTGCACCGGATGAAATTGATGTC
TTTGATTCAAACTTATTGAATGGCCAAGTGTGCTTTCATATAAGAGAACGTGAGAAGTTAAGAGCGATCTTGAATCTTGG
GTTTAAAGATGCGTTTAGAATATCTCACCCAAATTTGCAACAATTCACTTGGTGGCATTATCAAGGCAATTCACTCAGAA
ATAATCAAGGAATGCGAATAGATTATATGCTGTTATCACCACAAGCTGTAGATAAATTGGAAACATGCTACATAGATGAT
AGATTGCGTAAGCTAGAAAATCCGTCTGACCATACTCCTGTTGTATGTGCTATAGAATAA

Upstream 100 bases:

>100_bases
ACATTGCTGATAGTTTAAAGTTAATCAAGTGGAAAAAAATTATAACATCGAGGTTGCCTACTGGGGAGAGTTTAATTGAT
ATAATTAATAAGGATTGCTG

Downstream 100 bases:

>100_bases
TAGCAGATCTCTTACACAATTCACTTTTAGCAAACCTGAGTAGTCTTACCTTTGCTGCTCTGTGGTTCTTCCCTTCGTTT
GTTTTCCTTTCCTGTCCAAG

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MLKIATWNVNSIRKRVNQLCSFIVDSQIDIVLLQEIKCTEEQFPYAEIEKLGYEYAIYGQVARNGVCVLSKYPILEKFKI
DIVEGYQEARYIECIIKHNNQKVRVGSVYVPNGQSPDSQAFEYKLKFFDNLYERMSTLLKNEELTIIAGDYNVAPDEIDV
FDSNLLNGQVCFHIREREKLRAILNLGFKDAFRISHPNLQQFTWWHYQGNSLRNNQGMRIDYMLLSPQAVDKLETCYIDD
RLRKLENPSDHTPVVCAIE

Sequences:

>Translated_259_residues
MLKIATWNVNSIRKRVNQLCSFIVDSQIDIVLLQEIKCTEEQFPYAEIEKLGYEYAIYGQVARNGVCVLSKYPILEKFKI
DIVEGYQEARYIECIIKHNNQKVRVGSVYVPNGQSPDSQAFEYKLKFFDNLYERMSTLLKNEELTIIAGDYNVAPDEIDV
FDSNLLNGQVCFHIREREKLRAILNLGFKDAFRISHPNLQQFTWWHYQGNSLRNNQGMRIDYMLLSPQAVDKLETCYIDD
RLRKLENPSDHTPVVCAIE
>Mature_259_residues
MLKIATWNVNSIRKRVNQLCSFIVDSQIDIVLLQEIKCTEEQFPYAEIEKLGYEYAIYGQVARNGVCVLSKYPILEKFKI
DIVEGYQEARYIECIIKHNNQKVRVGSVYVPNGQSPDSQAFEYKLKFFDNLYERMSTLLKNEELTIIAGDYNVAPDEIDV
FDSNLLNGQVCFHIREREKLRAILNLGFKDAFRISHPNLQQFTWWHYQGNSLRNNQGMRIDYMLLSPQAVDKLETCYIDD
RLRKLENPSDHTPVVCAIE

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=262, Percent_Identity=29.3893129770992, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI18375503, Length=262, Percent_Identity=29.3893129770992, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI18375501, Length=262, Percent_Identity=29.3893129770992, Blast_Score=91, Evalue=9e-19,
Organism=Escherichia coli, GI1788046, Length=262, Percent_Identity=31.6793893129771, Blast_Score=134, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI71989536, Length=265, Percent_Identity=29.4339622641509, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI221330655, Length=259, Percent_Identity=30.1158301158301, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17136678, Length=259, Percent_Identity=30.1158301158301, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30232; Mature: 30232

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIATWNVNSIRKRVNQLCSFIVDSQIDIVLLQEIKCTEEQFPYAEIEKLGYEYAIYGQ
CEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHCCHHHHHHCCCEEEEEEE
VARNGVCVLSKYPILEKFKIDIVEGYQEARYIECIIKHNNQKVRVGSVYVPNGQSPDSQA
HHCCCEEEEECCCCHHHEEHHHHHHHHHHHEEEEEEECCCCEEEEEEEECCCCCCCCCHH
FEYKLKFFDNLYERMSTLLKNEELTIIAGDYNVAPDEIDVFDSNLLNGQVCFHIREREKL
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEECCCCCCCCCEEEEEEHHHHHH
RAILNLGFKDAFRISHPNLQQFTWWHYQGNSLRNNQGMRIDYMLLSPQAVDKLETCYIDD
HHHHHCCCCHHEECCCCCCCCEEEEEEECCCCCCCCCCEEEEEEECCHHHHHHHHHHHHH
RLRKLENPSDHTPVVCAIE
HHHHCCCCCCCCCEEEEEC
>Mature Secondary Structure
MLKIATWNVNSIRKRVNQLCSFIVDSQIDIVLLQEIKCTEEQFPYAEIEKLGYEYAIYGQ
CEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHCCHHHHHHCCCEEEEEEE
VARNGVCVLSKYPILEKFKIDIVEGYQEARYIECIIKHNNQKVRVGSVYVPNGQSPDSQA
HHCCCEEEEECCCCHHHEEHHHHHHHHHHHEEEEEEECCCCEEEEEEEECCCCCCCCCHH
FEYKLKFFDNLYERMSTLLKNEELTIIAGDYNVAPDEIDVFDSNLLNGQVCFHIREREKL
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEECCCCCCCCCEEEEEEHHHHHH
RAILNLGFKDAFRISHPNLQQFTWWHYQGNSLRNNQGMRIDYMLLSPQAVDKLETCYIDD
HHHHHCCCCHHEECCCCCCCCEEEEEEECCCCCCCCCCEEEEEEECCHHHHHHHHHHHHH
RLRKLENPSDHTPVVCAIE
HHHHCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]