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The map label for this gene is nuoF [H]

Identifier: 225630643

GI number: 225630643

Start: 995305

End: 996558

Strand: Direct

Name: nuoF [H]

Synonym: WRi_009240

Alternate gene names: 225630643

Gene position: 995305-996558 (Clockwise)

Preceding gene: 225630639

Following gene: 225630647

Centisome position: 68.84

GC content: 39.79

Gene sequence:

>1254_bases
ATGCTAAAAGAACAAGACAAAATATTCACCAACCTAAATGGTAAAGAAACTCCACTACTTGAGGGTGCAAAAAAACGTGG
TGGTTGGCAAAAAACAAAGGAATTACTGGATTTAGGATCAGAAAAAATCATTGATGAAGTAAAGAAATCTGGCTTGAGAG
GTCGAGGGGGTGCAGGATTTTCCACCGGCCTTAAGTGGAGCTTTATGCCCAAAAATCTCCCAAAAGCATATTTAGTAGTC
AATGCAGATGAGTCAGAACCTGGTACATGTAAAGATAGAGATATATTGCGATATGAGCCACATAAGTTACTTGAGGGAAT
TCTCCTGGCCAGCAGAGCGATCAGCGCATCAGTTGCGTATATTTATATCAGGGGTGAATTTTATAATGAATATTTAGTTC
TGAAAAAAGCACTTGAGGAAGCCTATAAAGAAAACTTAATTGGAAAAAATGCCTGCAAATCAGGTTATGATCTTGATGTG
TTTATCCATAGGGGTGCAGGAGCTTACATATGTGGAGAAGAAACAGCTCAACTTGAATCAATTGAAGGAAAAAAGGGCTT
TCCTCGCATGAAGCCTCCATTTCCCGCAGGTGTTGGACTTTTTGGCTGCCCAACCACAATAAACAACGTTGAAACTATAG
CCATGGTTCCAGATATTCTAAATCGCGGAGGAGAATGGTTTACATCTCTTGGTAAACCAAATAATACTGGTACCAAGGTC
TTTTGTATTTCAGGACATGTAAACAACCCGTGTAATGTTGAAGAAGAGCTCGGAATTCCATTACGTGAATTAATTGAAAA
ATACGCAGGTGGAGTGCGAGGAGGTTGGGATAATTTACTTGCTGTAATACCTGGTGGGTCTTCAGCGCCATTAATTCCAA
AATCTATATGCGATACTATTGAAATGGATTTTGATTCATTAAGAACTGCACAATCAGGGCTTGGTACTGCTGCTGTAATA
GTGATGGATAAATCAACTGATATAATAGCTGCAATAGAGAGGTTATCACACTTTTATATGCATGAGTCCTGTGGACAATG
CACACCATGCCGTGAAGGTACTGGATGGATGTGGAGGATTATGAAGAGGATGGTGGCAGGAGATATTAAACCTGATGAAA
TAGATAAGCTGCTTGACCTTACAACTCAAATAGAAGGACATACAATTTGCGCGCTTGGCGATGCTGCAGCTTGGCCGATT
CAAGGATTAATAAGACATTTTCGCCATGTTATCAAAGAGAGAGCGATAATTTAA

Upstream 100 bases:

>100_bases
AATGAAATGATCCCGTAGCTCAGCCGGTAGAGCAACTGACTTTTAATCAGTGGGTCACGCGTTCGAATCGCGTCGGGATC
ACTCACAAATTTTGAAAAAC

Downstream 100 bases:

>100_bases
CTTTACTTATACTTCTTAAGCGTATCCGTTCAGCGGAGTGGCAGAATAAGGTAGACAAGGTGATCTAAAAAGATAATCAT
AGAGCAAAAGTGAAATAATA

Product: NADH dehydrogenase I subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F; NDH-1 subunit F [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MLKEQDKIFTNLNGKETPLLEGAKKRGGWQKTKELLDLGSEKIIDEVKKSGLRGRGGAGFSTGLKWSFMPKNLPKAYLVV
NADESEPGTCKDRDILRYEPHKLLEGILLASRAISASVAYIYIRGEFYNEYLVLKKALEEAYKENLIGKNACKSGYDLDV
FIHRGAGAYICGEETAQLESIEGKKGFPRMKPPFPAGVGLFGCPTTINNVETIAMVPDILNRGGEWFTSLGKPNNTGTKV
FCISGHVNNPCNVEEELGIPLRELIEKYAGGVRGGWDNLLAVIPGGSSAPLIPKSICDTIEMDFDSLRTAQSGLGTAAVI
VMDKSTDIIAAIERLSHFYMHESCGQCTPCREGTGWMWRIMKRMVAGDIKPDEIDKLLDLTTQIEGHTICALGDAAAWPI
QGLIRHFRHVIKERAII

Sequences:

>Translated_417_residues
MLKEQDKIFTNLNGKETPLLEGAKKRGGWQKTKELLDLGSEKIIDEVKKSGLRGRGGAGFSTGLKWSFMPKNLPKAYLVV
NADESEPGTCKDRDILRYEPHKLLEGILLASRAISASVAYIYIRGEFYNEYLVLKKALEEAYKENLIGKNACKSGYDLDV
FIHRGAGAYICGEETAQLESIEGKKGFPRMKPPFPAGVGLFGCPTTINNVETIAMVPDILNRGGEWFTSLGKPNNTGTKV
FCISGHVNNPCNVEEELGIPLRELIEKYAGGVRGGWDNLLAVIPGGSSAPLIPKSICDTIEMDFDSLRTAQSGLGTAAVI
VMDKSTDIIAAIERLSHFYMHESCGQCTPCREGTGWMWRIMKRMVAGDIKPDEIDKLLDLTTQIEGHTICALGDAAAWPI
QGLIRHFRHVIKERAII
>Mature_417_residues
MLKEQDKIFTNLNGKETPLLEGAKKRGGWQKTKELLDLGSEKIIDEVKKSGLRGRGGAGFSTGLKWSFMPKNLPKAYLVV
NADESEPGTCKDRDILRYEPHKLLEGILLASRAISASVAYIYIRGEFYNEYLVLKKALEEAYKENLIGKNACKSGYDLDV
FIHRGAGAYICGEETAQLESIEGKKGFPRMKPPFPAGVGLFGCPTTINNVETIAMVPDILNRGGEWFTSLGKPNNTGTKV
FCISGHVNNPCNVEEELGIPLRELIEKYAGGVRGGWDNLLAVIPGGSSAPLIPKSICDTIEMDFDSLRTAQSGLGTAAVI
VMDKSTDIIAAIERLSHFYMHESCGQCTPCREGTGWMWRIMKRMVAGDIKPDEIDKLLDLTTQIEGHTICALGDAAAWPI
QGLIRHFRHVIKERAII

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=415, Percent_Identity=68.1927710843374, Blast_Score=593, Evalue=1e-169,
Organism=Homo sapiens, GI260656005, Length=415, Percent_Identity=68.1927710843374, Blast_Score=593, Evalue=1e-169,
Organism=Escherichia coli, GI1788620, Length=399, Percent_Identity=42.1052631578947, Blast_Score=322, Evalue=4e-89,
Organism=Caenorhabditis elegans, GI17531727, Length=416, Percent_Identity=64.9038461538462, Blast_Score=578, Evalue=1e-165,
Organism=Drosophila melanogaster, GI19920794, Length=415, Percent_Identity=66.5060240963855, Blast_Score=588, Evalue=1e-168,
Organism=Drosophila melanogaster, GI45550452, Length=416, Percent_Identity=62.2596153846154, Blast_Score=540, Evalue=1e-154,
Organism=Drosophila melanogaster, GI24653819, Length=420, Percent_Identity=49.047619047619, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24653817, Length=420, Percent_Identity=45.2380952380952, Blast_Score=358, Evalue=3e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 45863; Mature: 45863

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEQDKIFTNLNGKETPLLEGAKKRGGWQKTKELLDLGSEKIIDEVKKSGLRGRGGAGF
CCCCCCHHHHCCCCCCCCHHHCHHHCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
STGLKWSFMPKNLPKAYLVVNADESEPGTCKDRDILRYEPHKLLEGILLASRAISASVAY
CCCCEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHCEEEE
IYIRGEFYNEYLVLKKALEEAYKENLIGKNACKSGYDLDVFIHRGAGAYICGEETAQLES
EEEECCHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCEEEEEECCCCEEEECCCHHHHHH
IEGKKGFPRMKPPFPAGVGLFGCPTTINNVETIAMVPDILNRGGEWFTSLGKPNNTGTKV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHCCCHHHHHCCCCCCCCCEE
FCISGHVNNPCNVEEELGIPLRELIEKYAGGVRGGWDNLLAVIPGGSSAPLIPKSICDTI
EEEECCCCCCCCCHHHCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHH
EMDFDSLRTAQSGLGTAAVIVMDKSTDIIAAIERLSHFYMHESCGQCTPCREGTGWMWRI
HCCHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
MKRMVAGDIKPDEIDKLLDLTTQIEGHTICALGDAAAWPIQGLIRHFRHVIKERAII
HHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKEQDKIFTNLNGKETPLLEGAKKRGGWQKTKELLDLGSEKIIDEVKKSGLRGRGGAGF
CCCCCCHHHHCCCCCCCCHHHCHHHCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
STGLKWSFMPKNLPKAYLVVNADESEPGTCKDRDILRYEPHKLLEGILLASRAISASVAY
CCCCEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHCEEEE
IYIRGEFYNEYLVLKKALEEAYKENLIGKNACKSGYDLDVFIHRGAGAYICGEETAQLES
EEEECCHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCEEEEEECCCCEEEECCCHHHHHH
IEGKKGFPRMKPPFPAGVGLFGCPTTINNVETIAMVPDILNRGGEWFTSLGKPNNTGTKV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHCCCHHHHHCCCCCCCCCEE
FCISGHVNNPCNVEEELGIPLRELIEKYAGGVRGGWDNLLAVIPGGSSAPLIPKSICDTI
EEEECCCCCCCCCHHHCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHH
EMDFDSLRTAQSGLGTAAVIVMDKSTDIIAAIERLSHFYMHESCGQCTPCREGTGWMWRI
HCCHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
MKRMVAGDIKPDEIDKLLDLTTQIEGHTICALGDAAAWPIQGLIRHFRHVIKERAII
HHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA