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The map label for this gene is trmE [H]

Identifier: 225630647

GI number: 225630647

Start: 1001429

End: 1002757

Strand: Direct

Name: trmE [H]

Synonym: WRi_009300

Alternate gene names: 225630647

Gene position: 1001429-1002757 (Clockwise)

Preceding gene: 225630643

Following gene: 225630648

Centisome position: 69.26

GC content: 35.89

Gene sequence:

>1329_bases
ATGACAAACACAAATGAAACTATTTTCGCTTTATCGACCGTATTTGGTAAGTCAGGAGTTGCAGTAATCAGAATTTCAGG
CAATTACGCGCTTAAAGCTTTAAATCATTTTCATATTAAGAAAGAAATTAAACCAAGATTTGCTACTTTAGTTGATCTAT
ATGATGATTCCAGTCAATTGATAGATAATGGAATAATCATCTATTTCCCTGCTCCAAACAGTTTCACTGGCGAGGATGTT
ATAGAGTTACAAGTGCATGGAAGCAAGGCAGTTATAAAAATCATCTTGGAGGAATTATCAAAAGTTTTCGTTATGGCCAA
GCCTGGAGAATTCTCACTTAGGGCTTTTCTAAATGGTAAATTTGACTTAACGCAAATAGAAGGGATTGCAGACTTAATTG
ATGCTGAGACGAAAATGCAAGCTAAACAAGCGATTAAGCAGATATCAGGAGAATTGGAGAGACTATACAGCAATTGGAGG
CAAAGATTGATAACGATACAATCCAAAATCGAAGCATATATAGACTTTCCAGAGGACATTTGGGCAGAAAAAAGTGAGTT
GGAAAAAATTAATAATGAAGTGCAATCTCTCGTGCGATTGATACAAGAGCATTTAAATGATAATAGACGGGGCGAAAGGT
TGCGTGAGGGTTTACATATTGTAATAACTGGTGAACCAAATGTCGGTAAATCAACTCTGTTTAATTTCTTAGCCAAGCGT
GATATTGCTATTGTTTCTGAATATGCAGGCACAACAAGAGATATACTTGAAGCTCATATTGACATTGGCGGATACCCAAT
CATTCTCTCTGATACTGCTGGAATTCGTGAAAGTTCAGATCCAATAGAATTAGAAGGTATAAGTCGAGCGAAAAAGAGGT
CTTTTGAAGCTGATTTAAGAATAGAACTATTTCCTTTTGAACAGCGTCCTAATATCAATTGCAACGTTGTAAATAGCGAT
ACTATTTATGTATTGAGCAAAGCTGACGATGTTATCAATAATCACGATATAAAAATTAATGGCATAGATCTTTTACCCGT
TTCTATTCTAAAGGGAATAGGTACAAACAAATTGATCTCTCTCATAAAAGAGAAGGCAGAGGAAAAATTTGGGCATGATA
GAGACACTCCTGTGATTACTCGGCAAAGACATAGGAATCACATGCAGAAAGCACTGGAACATTTACAACGTTTTAATATC
GATAATCCAATTGAGTTGATATCTGAAGATTTGAGGCTTGCTGCATTTGAACTTGGTGCGGTGATTGGAATTATTGATGT
TGAGGAAATATTGAGTAGTGTGTTTAGCAACTTTTGCGTGGGCAAGTAA

Upstream 100 bases:

>100_bases
AATCATATTATCAGTTTTCAAAATAAATATTTTCACCTGGGTGACTTTTTAGGTATTTTGGTTCCTTAAGTATAACATTG
CTAAGTAGTTTATATAAATA

Downstream 100 bases:

>100_bases
AGCTTTTATAGATTGCTTAAAATCAAATGTTGTGATAGAAAAGAAATGGACGGATGGCCGAGCGGTTTAAGGCACCAGTC
TTGAAAACTGACGTACGTGA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MTNTNETIFALSTVFGKSGVAVIRISGNYALKALNHFHIKKEIKPRFATLVDLYDDSSQLIDNGIIIYFPAPNSFTGEDV
IELQVHGSKAVIKIILEELSKVFVMAKPGEFSLRAFLNGKFDLTQIEGIADLIDAETKMQAKQAIKQISGELERLYSNWR
QRLITIQSKIEAYIDFPEDIWAEKSELEKINNEVQSLVRLIQEHLNDNRRGERLREGLHIVITGEPNVGKSTLFNFLAKR
DIAIVSEYAGTTRDILEAHIDIGGYPIILSDTAGIRESSDPIELEGISRAKKRSFEADLRIELFPFEQRPNINCNVVNSD
TIYVLSKADDVINNHDIKINGIDLLPVSILKGIGTNKLISLIKEKAEEKFGHDRDTPVITRQRHRNHMQKALEHLQRFNI
DNPIELISEDLRLAAFELGAVIGIIDVEEILSSVFSNFCVGK

Sequences:

>Translated_442_residues
MTNTNETIFALSTVFGKSGVAVIRISGNYALKALNHFHIKKEIKPRFATLVDLYDDSSQLIDNGIIIYFPAPNSFTGEDV
IELQVHGSKAVIKIILEELSKVFVMAKPGEFSLRAFLNGKFDLTQIEGIADLIDAETKMQAKQAIKQISGELERLYSNWR
QRLITIQSKIEAYIDFPEDIWAEKSELEKINNEVQSLVRLIQEHLNDNRRGERLREGLHIVITGEPNVGKSTLFNFLAKR
DIAIVSEYAGTTRDILEAHIDIGGYPIILSDTAGIRESSDPIELEGISRAKKRSFEADLRIELFPFEQRPNINCNVVNSD
TIYVLSKADDVINNHDIKINGIDLLPVSILKGIGTNKLISLIKEKAEEKFGHDRDTPVITRQRHRNHMQKALEHLQRFNI
DNPIELISEDLRLAAFELGAVIGIIDVEEILSSVFSNFCVGK
>Mature_441_residues
TNTNETIFALSTVFGKSGVAVIRISGNYALKALNHFHIKKEIKPRFATLVDLYDDSSQLIDNGIIIYFPAPNSFTGEDVI
ELQVHGSKAVIKIILEELSKVFVMAKPGEFSLRAFLNGKFDLTQIEGIADLIDAETKMQAKQAIKQISGELERLYSNWRQ
RLITIQSKIEAYIDFPEDIWAEKSELEKINNEVQSLVRLIQEHLNDNRRGERLREGLHIVITGEPNVGKSTLFNFLAKRD
IAIVSEYAGTTRDILEAHIDIGGYPIILSDTAGIRESSDPIELEGISRAKKRSFEADLRIELFPFEQRPNINCNVVNSDT
IYVLSKADDVINNHDIKINGIDLLPVSILKGIGTNKLISLIKEKAEEKFGHDRDTPVITRQRHRNHMQKALEHLQRFNID
NPIELISEDLRLAAFELGAVIGIIDVEEILSSVFSNFCVGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=463, Percent_Identity=39.7408207343413, Blast_Score=325, Evalue=7e-89,
Organism=Homo sapiens, GI193082993, Length=448, Percent_Identity=40.4017857142857, Blast_Score=316, Evalue=3e-86,
Organism=Homo sapiens, GI193082988, Length=495, Percent_Identity=37.1717171717172, Blast_Score=309, Evalue=4e-84,
Organism=Escherichia coli, GI2367268, Length=455, Percent_Identity=35.6043956043956, Blast_Score=259, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17507259, Length=457, Percent_Identity=33.2603938730853, Blast_Score=197, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6323665, Length=489, Percent_Identity=37.2188139059305, Blast_Score=259, Evalue=7e-70,
Organism=Drosophila melanogaster, GI45550813, Length=476, Percent_Identity=38.4453781512605, Blast_Score=307, Evalue=1e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49817; Mature: 49686

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNTNETIFALSTVFGKSGVAVIRISGNYALKALNHFHIKKEIKPRFATLVDLYDDSSQL
CCCCCCEEEEEEHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHH
IDNGIIIYFPAPNSFTGEDVIELQVHGSKAVIKIILEELSKVFVMAKPGEFSLRAFLNGK
HHCCEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHEEEEECCCCCEEEEEECCC
FDLTQIEGIADLIDAETKMQAKQAIKQISGELERLYSNWRQRLITIQSKIEAYIDFPEDI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
WAEKSELEKINNEVQSLVRLIQEHLNDNRRGERLREGLHIVITGEPNVGKSTLFNFLAKR
HCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEEEECCCCCCHHHHHHHHHHC
DIAIVSEYAGTTRDILEAHIDIGGYPIILSDTAGIRESSDPIELEGISRAKKRSFEADLR
CHHHHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEECCCCHHHHCCCCCCEE
IELFPFEQRPNINCNVVNSDTIYVLSKADDVINNHDIKINGIDLLPVSILKGIGTNKLIS
EEEEECCCCCCCEEEEECCCEEEEEECCCCCCCCCCEEEECEEEHHHHHHCCCCHHHHHH
LIKEKAEEKFGHDRDTPVITRQRHRNHMQKALEHLQRFNIDNPIELISEDLRLAAFELGA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VIGIIDVEEILSSVFSNFCVGK
HHEEECHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TNTNETIFALSTVFGKSGVAVIRISGNYALKALNHFHIKKEIKPRFATLVDLYDDSSQL
CCCCCEEEEEEHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHH
IDNGIIIYFPAPNSFTGEDVIELQVHGSKAVIKIILEELSKVFVMAKPGEFSLRAFLNGK
HHCCEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHEEEEECCCCCEEEEEECCC
FDLTQIEGIADLIDAETKMQAKQAIKQISGELERLYSNWRQRLITIQSKIEAYIDFPEDI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
WAEKSELEKINNEVQSLVRLIQEHLNDNRRGERLREGLHIVITGEPNVGKSTLFNFLAKR
HCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEEEECCCCCCHHHHHHHHHHC
DIAIVSEYAGTTRDILEAHIDIGGYPIILSDTAGIRESSDPIELEGISRAKKRSFEADLR
CHHHHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEECCCCHHHHCCCCCCEE
IELFPFEQRPNINCNVVNSDTIYVLSKADDVINNHDIKINGIDLLPVSILKGIGTNKLIS
EEEEECCCCCCCEEEEECCCEEEEEECCCCCCCCCCEEEECEEEHHHHHHCCCCHHHHHH
LIKEKAEEKFGHDRDTPVITRQRHRNHMQKALEHLQRFNIDNPIELISEDLRLAAFELGA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VIGIIDVEEILSSVFSNFCVGK
HHEEECHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA