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The map label for this gene is uvrD [H]

Identifier: 225630630

GI number: 225630630

Start: 982483

End: 984399

Strand: Reverse

Name: uvrD [H]

Synonym: WRi_009090

Alternate gene names: 225630630

Gene position: 984399-982483 (Counterclockwise)

Preceding gene: 225630631

Following gene: 225630625

Centisome position: 68.08

GC content: 35.89

Gene sequence:

>1917_bases
ATGAACGATTATCTCTCACTGCTAAATCCAGAACAACAATCAGCTGTAACTAACATAGATGGACCGGTTTTAATATTGGC
AGGAGCCGGAACCGGAAAAACAAGAACGATTACTTCAAGAATAGCACACATAATTAGAAATGGTCACGCTTATTCTGATG
AAATATTAGCTGTTACATTCACAAATAAAGCAGCAAATGAGATGGTATCAAGGGTACTTGAGCTAACGGGCACAAACATA
CCATGGCTTGGCACTTTCCATGCAATTGCAGCAAAAATTTTGCGCCTGCACGCAGAAATTGTGGGCTTAAACCCCAATTT
TACAATCATTGGTGTGGATGACCAATTACAGGTAATAAAAAATATTATCAATGAAATAAATCCTGATTACCTATCAGAAA
AATGTAAGACCATTATGAATATTATTCAGCAATGGAAAGAGAAGTGCTTATTGCCATCTGAAGTGGAAGATATTCAATCA
TTTAGGCCAGTATATGTAACTGCACTCAAGGTCTATCACCAGTATCAGGAAAGGTTGAGATTCCTTAACTCTGTCGATTT
TGGTGACTTATTGCTATATAACATACAACTCTTTAATCAAAAGACCGAAGTTCTGTCCTACTACCAAAACAAGTTTAAAT
ATGTCATGGTAGATGAATATCAAGATACAAATGCGATACAATATCTTTGGCTAAAATACCTTGCAAAAGAGCACTCAAAT
ATTTGCTGTGTAGGAGATGATGACCAGTCGATATATAGTTGGCGTGGTGCAGAAGTTGAAAATATTTTGAAGTTTTCTGA
TGATTTTAAAAATGCAAAAACCGTTAAACTAGAGTGTAACTACAGGTCAACATCCCACATACTTGCAACTGCGTCATATG
TTATCAATCACAATAAGACTCGCTTAGAAAAAAAATTGTGGACAACAAACATTGAAGGGGAAAAGGTAAATCTAATAAAA
TTATGGGACGGAAAAGCTGAAGCAAGGTTCATAAGCGAACAGATATTAAAGCTCAATAAATACAGATTTAGTGACATTGC
AGTGCTGGTAAGGGCCACTTTTCAAACTAGAGTTCTCGAAGAGTATTTTATAAAATATTCAATTCCCTACAAGATTATAA
GTGGCGTAAAATTCTACGAACGTCAGGAAGTCAGGGATATAATTGCATATTTAAGACTCGTTACAAACAATAACGATGAC
TTAGCTTTCGAAAGGATCGTAAACCGGCCAAAAAGAAGCATAGGAGCTACAACTCTAAAGAAAATATACACGACTGCTCA
GGATAACAAAATTTCTTTTTTTGAGGCAGCAAAAATATTAGTTAATAGTAATCAAGTAACCGAAAGAATTAAACTCTCAC
TAAATGATTTTTTAAATAAAATTAAAGCTTGGGAAGAAATAGTAAGCGTAAAACCACTGCATGAATTCGTTAAAATCGTA
GCAAACCAATCAGGGTATATTGAAATGCTTGAAAATGAGGAGGTAACAGGCTTAGCACGAATAGAAAATGTCAAGGAGCT
CATCTCATCTTTAAAAAATTTCGATAATGCCACAACTTTCTTGGAGCACATAAGTTTGGTGATGGAAGTGGATAATATGA
ATAACGATGACACCGTATATGTTATGACTCTTCATGCAGCCAAAGGACTTGAATTCCCGTGCGTGTTTCTACCTGGTTGG
GAAGAGGGATTATTTCCTCATCAAAGATCTTTTGATGACAAAAGTGGTAAAGCTTTAGAAGAAGAAAGGAGACTCGCATA
TGTTGGCATAACCAGAGCCAAAGAAAGGTTAATCATTTCATGTGCAGATAGGAGGGAAATTAACAACCAGTGGCAACCGA
TGCGCACTTCTCGGTTCATTAAAGAATTGCCAAGAGAAAATGTTGAGGTAATTAAAAGCAACATTGCCTACTGTTGA

Upstream 100 bases:

>100_bases
TACTTGAGTGAAGCATTCTTTAACTGTTCTATTCAATAAAACCTTTACAATATTTCAATCATAGGTTTAACTTTACGAAG
TTATAATCTATAGGGCTACG

Downstream 100 bases:

>100_bases
AGCTATAATTGCAGATAAACATATCTTACAAATCTGAATTACTTTAACTATATGTTTCTCCTGGCAAAAATTCTGTAACC
GTTCACACTTAATTCTTCAA

Product: DNA helicase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 638; Mature: 638

Protein sequence:

>638_residues
MNDYLSLLNPEQQSAVTNIDGPVLILAGAGTGKTRTITSRIAHIIRNGHAYSDEILAVTFTNKAANEMVSRVLELTGTNI
PWLGTFHAIAAKILRLHAEIVGLNPNFTIIGVDDQLQVIKNIINEINPDYLSEKCKTIMNIIQQWKEKCLLPSEVEDIQS
FRPVYVTALKVYHQYQERLRFLNSVDFGDLLLYNIQLFNQKTEVLSYYQNKFKYVMVDEYQDTNAIQYLWLKYLAKEHSN
ICCVGDDDQSIYSWRGAEVENILKFSDDFKNAKTVKLECNYRSTSHILATASYVINHNKTRLEKKLWTTNIEGEKVNLIK
LWDGKAEARFISEQILKLNKYRFSDIAVLVRATFQTRVLEEYFIKYSIPYKIISGVKFYERQEVRDIIAYLRLVTNNNDD
LAFERIVNRPKRSIGATTLKKIYTTAQDNKISFFEAAKILVNSNQVTERIKLSLNDFLNKIKAWEEIVSVKPLHEFVKIV
ANQSGYIEMLENEEVTGLARIENVKELISSLKNFDNATTFLEHISLVMEVDNMNNDDTVYVMTLHAAKGLEFPCVFLPGW
EEGLFPHQRSFDDKSGKALEEERRLAYVGITRAKERLIISCADRREINNQWQPMRTSRFIKELPRENVEVIKSNIAYC

Sequences:

>Translated_638_residues
MNDYLSLLNPEQQSAVTNIDGPVLILAGAGTGKTRTITSRIAHIIRNGHAYSDEILAVTFTNKAANEMVSRVLELTGTNI
PWLGTFHAIAAKILRLHAEIVGLNPNFTIIGVDDQLQVIKNIINEINPDYLSEKCKTIMNIIQQWKEKCLLPSEVEDIQS
FRPVYVTALKVYHQYQERLRFLNSVDFGDLLLYNIQLFNQKTEVLSYYQNKFKYVMVDEYQDTNAIQYLWLKYLAKEHSN
ICCVGDDDQSIYSWRGAEVENILKFSDDFKNAKTVKLECNYRSTSHILATASYVINHNKTRLEKKLWTTNIEGEKVNLIK
LWDGKAEARFISEQILKLNKYRFSDIAVLVRATFQTRVLEEYFIKYSIPYKIISGVKFYERQEVRDIIAYLRLVTNNNDD
LAFERIVNRPKRSIGATTLKKIYTTAQDNKISFFEAAKILVNSNQVTERIKLSLNDFLNKIKAWEEIVSVKPLHEFVKIV
ANQSGYIEMLENEEVTGLARIENVKELISSLKNFDNATTFLEHISLVMEVDNMNNDDTVYVMTLHAAKGLEFPCVFLPGW
EEGLFPHQRSFDDKSGKALEEERRLAYVGITRAKERLIISCADRREINNQWQPMRTSRFIKELPRENVEVIKSNIAYC
>Mature_638_residues
MNDYLSLLNPEQQSAVTNIDGPVLILAGAGTGKTRTITSRIAHIIRNGHAYSDEILAVTFTNKAANEMVSRVLELTGTNI
PWLGTFHAIAAKILRLHAEIVGLNPNFTIIGVDDQLQVIKNIINEINPDYLSEKCKTIMNIIQQWKEKCLLPSEVEDIQS
FRPVYVTALKVYHQYQERLRFLNSVDFGDLLLYNIQLFNQKTEVLSYYQNKFKYVMVDEYQDTNAIQYLWLKYLAKEHSN
ICCVGDDDQSIYSWRGAEVENILKFSDDFKNAKTVKLECNYRSTSHILATASYVINHNKTRLEKKLWTTNIEGEKVNLIK
LWDGKAEARFISEQILKLNKYRFSDIAVLVRATFQTRVLEEYFIKYSIPYKIISGVKFYERQEVRDIIAYLRLVTNNNDD
LAFERIVNRPKRSIGATTLKKIYTTAQDNKISFFEAAKILVNSNQVTERIKLSLNDFLNKIKAWEEIVSVKPLHEFVKIV
ANQSGYIEMLENEEVTGLARIENVKELISSLKNFDNATTFLEHISLVMEVDNMNNDDTVYVMTLHAAKGLEFPCVFLPGW
EEGLFPHQRSFDDKSGKALEEERRLAYVGITRAKERLIISCADRREINNQWQPMRTSRFIKELPRENVEVIKSNIAYC

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=648, Percent_Identity=42.2839506172839, Blast_Score=481, Evalue=1e-137,
Organism=Escherichia coli, GI48994965, Length=645, Percent_Identity=36.8992248062016, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1787196, Length=90, Percent_Identity=41.1111111111111, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322369, Length=459, Percent_Identity=32.6797385620915, Blast_Score=182, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6324477, Length=711, Percent_Identity=23.0661040787623, Blast_Score=90, Evalue=9e-19,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 73734; Mature: 73734

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDYLSLLNPEQQSAVTNIDGPVLILAGAGTGKTRTITSRIAHIIRNGHAYSDEILAVTF
CCCCHHHCCCCHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEE
TNKAANEMVSRVLELTGTNIPWLGTFHAIAAKILRLHAEIVGLNPNFTIIGVDDQLQVIK
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCEEEEECCHHHHHHH
NIINEINPDYLSEKCKTIMNIIQQWKEKCLLPSEVEDIQSFRPVYVTALKVYHQYQERLR
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHH
FLNSVDFGDLLLYNIQLFNQKTEVLSYYQNKFKYVMVDEYQDTNAIQYLWLKYLAKEHSN
HHHCCCCCHHHEEEHHHHCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCC
ICCVGDDDQSIYSWRGAEVENILKFSDDFKNAKTVKLECNYRSTSHILATASYVINHNKT
EEEECCCCCHHHHCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCHH
RLEKKLWTTNIEGEKVNLIKLWDGKAEARFISEQILKLNKYRFSDIAVLVRATFQTRVLE
HHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
EYFIKYSIPYKIISGVKFYERQEVRDIIAYLRLVTNNNDDLAFERIVNRPKRSIGATTLK
HHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHH
KIYTTAQDNKISFFEAAKILVNSNQVTERIKLSLNDFLNKIKAWEEIVSVKPLHEFVKIV
HHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANQSGYIEMLENEEVTGLARIENVKELISSLKNFDNATTFLEHISLVMEVDNMNNDDTVY
HCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEECCCCCCCEEE
VMTLHAAKGLEFPCVFLPGWEEGLFPHQRSFDDKSGKALEEERRLAYVGITRAKERLIIS
EEEEECCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHCCEEEEEHHHHCCEEEEE
CADRREINNQWQPMRTSRFIKELPRENVEVIKSNIAYC
ECCHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MNDYLSLLNPEQQSAVTNIDGPVLILAGAGTGKTRTITSRIAHIIRNGHAYSDEILAVTF
CCCCHHHCCCCHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEE
TNKAANEMVSRVLELTGTNIPWLGTFHAIAAKILRLHAEIVGLNPNFTIIGVDDQLQVIK
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCEEEEECCHHHHHHH
NIINEINPDYLSEKCKTIMNIIQQWKEKCLLPSEVEDIQSFRPVYVTALKVYHQYQERLR
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHH
FLNSVDFGDLLLYNIQLFNQKTEVLSYYQNKFKYVMVDEYQDTNAIQYLWLKYLAKEHSN
HHHCCCCCHHHEEEHHHHCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCC
ICCVGDDDQSIYSWRGAEVENILKFSDDFKNAKTVKLECNYRSTSHILATASYVINHNKT
EEEECCCCCHHHHCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCHH
RLEKKLWTTNIEGEKVNLIKLWDGKAEARFISEQILKLNKYRFSDIAVLVRATFQTRVLE
HHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
EYFIKYSIPYKIISGVKFYERQEVRDIIAYLRLVTNNNDDLAFERIVNRPKRSIGATTLK
HHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHH
KIYTTAQDNKISFFEAAKILVNSNQVTERIKLSLNDFLNKIKAWEEIVSVKPLHEFVKIV
HHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANQSGYIEMLENEEVTGLARIENVKELISSLKNFDNATTFLEHISLVMEVDNMNNDDTVY
HCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEECCCCCCCEEE
VMTLHAAKGLEFPCVFLPGWEEGLFPHQRSFDDKSGKALEEERRLAYVGITRAKERLIIS
EEEEECCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHCCEEEEEHHHHCCEEEEE
CADRREINNQWQPMRTSRFIKELPRENVEVIKSNIAYC
ECCHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA