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The map label for this gene is rne [H]

Identifier: 225630610

GI number: 225630610

Start: 956496

End: 958268

Strand: Reverse

Name: rne [H]

Synonym: WRi_008810

Alternate gene names: 225630610

Gene position: 958268-956496 (Counterclockwise)

Preceding gene: 225630613

Following gene: 225630609

Centisome position: 66.28

GC content: 33.33

Gene sequence:

>1773_bases
GTGGTAAATAGTGGTAAAAGGTTATTATTGATAGAAAATTCCACCTGTTCTAATGAGGTAAGAGTTGCCTTGTCAGTTAA
TAATAAGGTTGTAGAATTTGAACAAGAATTCAAGGAAAAAAAACAATTAAGAGGTAATATATACGTTGCTTATATAAAGC
GTATAGAGCCTTCTTTGCAAGCCGTATTTATCGAATATGGAAAAAATAAGCAAGGTTTTTTGTCTTTTTCCGAGATATCT
CTAGATTACTTCAATATTCCAGAAAAAGAAAAGGAAACTATCTTTGAGAGCTACTCAAATGACGATTATACAGAAAAAGG
GAACGATACCATAGCAAATGTCTCTTCAGATTCTGCAGCTAGTAAGAATGTCAACGAGTCAGGTAGTGGTTTTGTAAGAG
AGGTACCTTTATATAAAAAATACAAATTACAAGATGTTATTTCAGTAAATCAAAAAGTATTGGTTCAGCTAACTAAAGAG
GAACGAGGCAATAAAGGAGCTTCATTTACAACATACATAACTTTAGTGGGTAGATACTGTGTTTTCATGCCAAATTCCAT
GAGTAAAGGTGGAGTGTCTCGCAGAATTGAAGATGCTAATGTCAGAAAGCAGTTAAAGGAGATACTGAGTTCAATAAACT
TGCCAAAAAGGTCAGGTTTAATAGTAAGAACTGTTGGTTCAGGAAAAAATAAGAAAGAAATTGAGCAGGATTACAATTAC
TTATCCTCATTATGGCAGAGCATTCAAAAAAATGCCTTCTCTGTAAACGTTCCATCATTAATTTACAATGAAGCAGATGT
AATTATGAGATCTGTTCGTGATTTTTGTAGCGATGGTATAGAAGTTATAGTGTCTGGAAAGGAAGCTTTTGAGGCAGTTA
GGCAATATGCTAACAATGCACTAAAAGGTAGTAAGTTGCGCTATAGATTGTATAGAGGTTATGCTCCGATCTTTACTTAC
TACGGAATCGAAGATCAAATTTCTGAGTTATATAGCAATAGAGTAGAATTGCCATCTGGTGGGTCTTTGATAATAACTTT
AACTGAGGCGTTTGTTTCAATAGACGTAAATTCAGGAAAGATGACAGGAGAAGATAGCATAGAAGAAACAGCTTATAGAA
CCAACATGGAAGCAGTACCTGAAATATCAAGACAGGTGAATCTTAGGGGCCTATCAGGATTAATAGTAGTCGATTTTATT
GATATGTTGAAATATCAGTATTGCAGAGCTGTTGAGTCTGCTATAAAGCAAGCGTTTAAAGATGATAAAGCTAAAGTTCA
ATTTAGCTACATAAATGACTTTGGTTTAATGGTATTTTCAAGACAAAGAATTAAACCAAACATACAAGAAATTAATACTA
CAGAGTGTTTACACTGCAAAGGTACTGGAAGAGTAAAATCAAACGAAGTAATTGTTTCATCAATACTAAGGGATTTACAA
CATATTGCTAACAAGAATCGAAATAAGTCTTTTGATTTAGTAGCACACAGTGCAGTTATAGCGCACATTTTTAATAACAA
ACGCAATACAGTTTCTACAATCGAAAAAGAGTTTAATATTACATTGAACGTTAGTATTGATAACAGTTTAGATGTAAATA
CATTTGTTTTAAAACAAGGAGATGATGTTAATTTAAACGGTTATAAGCCATTACAGAACTCTGGATACCAAGTTGGGGAT
AGCAGCAATGAAGAAGCTGATAATTCAAATAAACTACGAGGCAATTTTTGGCTGACTAAGTGGCTTTCGCGCCTTTTAAG
TTCTAATAACTAA

Upstream 100 bases:

>100_bases
AAAAGTCAACCGAATAATGCAGATCGTAAAATTCTTGACAATCAACAGTGCATTAAGTAGCATGAAGTTAACTTCAGAAT
GAAAAGCAAACGGTAAATAA

Downstream 100 bases:

>100_bases
TTAGAAACCTAAATTGGGCTTGTAATTAATGAGCCCTATACTTATATATTCTTATATCAGATAATATTTACAATGGGGTA
CTCATTATGGGAAGAGCAAT

Product: Ribonuclease, Rne/Rng family protein

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 590; Mature: 590

Protein sequence:

>590_residues
MVNSGKRLLLIENSTCSNEVRVALSVNNKVVEFEQEFKEKKQLRGNIYVAYIKRIEPSLQAVFIEYGKNKQGFLSFSEIS
LDYFNIPEKEKETIFESYSNDDYTEKGNDTIANVSSDSAASKNVNESGSGFVREVPLYKKYKLQDVISVNQKVLVQLTKE
ERGNKGASFTTYITLVGRYCVFMPNSMSKGGVSRRIEDANVRKQLKEILSSINLPKRSGLIVRTVGSGKNKKEIEQDYNY
LSSLWQSIQKNAFSVNVPSLIYNEADVIMRSVRDFCSDGIEVIVSGKEAFEAVRQYANNALKGSKLRYRLYRGYAPIFTY
YGIEDQISELYSNRVELPSGGSLIITLTEAFVSIDVNSGKMTGEDSIEETAYRTNMEAVPEISRQVNLRGLSGLIVVDFI
DMLKYQYCRAVESAIKQAFKDDKAKVQFSYINDFGLMVFSRQRIKPNIQEINTTECLHCKGTGRVKSNEVIVSSILRDLQ
HIANKNRNKSFDLVAHSAVIAHIFNNKRNTVSTIEKEFNITLNVSIDNSLDVNTFVLKQGDDVNLNGYKPLQNSGYQVGD
SSNEEADNSNKLRGNFWLTKWLSRLLSSNN

Sequences:

>Translated_590_residues
MVNSGKRLLLIENSTCSNEVRVALSVNNKVVEFEQEFKEKKQLRGNIYVAYIKRIEPSLQAVFIEYGKNKQGFLSFSEIS
LDYFNIPEKEKETIFESYSNDDYTEKGNDTIANVSSDSAASKNVNESGSGFVREVPLYKKYKLQDVISVNQKVLVQLTKE
ERGNKGASFTTYITLVGRYCVFMPNSMSKGGVSRRIEDANVRKQLKEILSSINLPKRSGLIVRTVGSGKNKKEIEQDYNY
LSSLWQSIQKNAFSVNVPSLIYNEADVIMRSVRDFCSDGIEVIVSGKEAFEAVRQYANNALKGSKLRYRLYRGYAPIFTY
YGIEDQISELYSNRVELPSGGSLIITLTEAFVSIDVNSGKMTGEDSIEETAYRTNMEAVPEISRQVNLRGLSGLIVVDFI
DMLKYQYCRAVESAIKQAFKDDKAKVQFSYINDFGLMVFSRQRIKPNIQEINTTECLHCKGTGRVKSNEVIVSSILRDLQ
HIANKNRNKSFDLVAHSAVIAHIFNNKRNTVSTIEKEFNITLNVSIDNSLDVNTFVLKQGDDVNLNGYKPLQNSGYQVGD
SSNEEADNSNKLRGNFWLTKWLSRLLSSNN
>Mature_590_residues
MVNSGKRLLLIENSTCSNEVRVALSVNNKVVEFEQEFKEKKQLRGNIYVAYIKRIEPSLQAVFIEYGKNKQGFLSFSEIS
LDYFNIPEKEKETIFESYSNDDYTEKGNDTIANVSSDSAASKNVNESGSGFVREVPLYKKYKLQDVISVNQKVLVQLTKE
ERGNKGASFTTYITLVGRYCVFMPNSMSKGGVSRRIEDANVRKQLKEILSSINLPKRSGLIVRTVGSGKNKKEIEQDYNY
LSSLWQSIQKNAFSVNVPSLIYNEADVIMRSVRDFCSDGIEVIVSGKEAFEAVRQYANNALKGSKLRYRLYRGYAPIFTY
YGIEDQISELYSNRVELPSGGSLIITLTEAFVSIDVNSGKMTGEDSIEETAYRTNMEAVPEISRQVNLRGLSGLIVVDFI
DMLKYQYCRAVESAIKQAFKDDKAKVQFSYINDFGLMVFSRQRIKPNIQEINTTECLHCKGTGRVKSNEVIVSSILRDLQ
HIANKNRNKSFDLVAHSAVIAHIFNNKRNTVSTIEKEFNITLNVSIDNSLDVNTFVLKQGDDVNLNGYKPLQNSGYQVGD
SSNEEADNSNKLRGNFWLTKWLSRLLSSNN

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. Major endoribonuclease participating in mRNA turnover. It initiates the decay of RNAs by cutting them internally near their 5'-end. It is able to remove poly(A) tails by an endonucleolytic proce

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=512, Percent_Identity=38.28125, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI87082235, Length=507, Percent_Identity=28.9940828402367, Blast_Score=207, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 66699; Mature: 66699

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNSGKRLLLIENSTCSNEVRVALSVNNKVVEFEQEFKEKKQLRGNIYVAYIKRIEPSLQ
CCCCCCEEEEEECCCCCCCEEEEEEECCCEEHHHHHHHHHHHHCCCEEEEEEECCCCCCE
AVFIEYGKNKQGFLSFSEISLDYFNIPEKEKETIFESYSNDDYTEKGNDTIANVSSDSAA
EEEEEECCCCCCCEEHHHEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCC
SKNVNESGSGFVREVPLYKKYKLQDVISVNQKVLVQLTKEERGNKGASFTTYITLVGRYC
CCCCCCCCCCCEEECCCHHHHHHHHHHHCCHHHEEEEHHHHCCCCCCCHHHHHHHHHHHH
VFMPNSMSKGGVSRRIEDANVRKQLKEILSSINLPKRSGLIVRTVGSGKNKKEIEQDYNY
EECCCCCCCCCCHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHH
LSSLWQSIQKNAFSVNVPSLIYNEADVIMRSVRDFCSDGIEVIVSGKEAFEAVRQYANNA
HHHHHHHHHCCCEEEECCHHHHCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCC
LKGSKLRYRLYRGYAPIFTYYGIEDQISELYSNRVELPSGGSLIITLTEAFVSIDVNSGK
CCCCCEEEHHHHCCCCHHEECCCHHHHHHHHHCCCCCCCCCEEEEEEEEEEEEEECCCCC
MTGEDSIEETAYRTNMEAVPEISRQVNLRGLSGLIVVDFIDMLKYQYCRAVESAIKQAFK
CCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
DDKAKVQFSYINDFGLMVFSRQRIKPNIQEINTTECLHCKGTGRVKSNEVIVSSILRDLQ
CCCCEEEEEEECCHHHHEEEHHHCCCCHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHH
HIANKNRNKSFDLVAHSAVIAHIFNNKRNTVSTIEKEFNITLNVSIDNSLDVNTFVLKQG
HHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHEEEEEEEEEECCCCCCEEEEEECC
DDVNLNGYKPLQNSGYQVGDSSNEEADNSNKLRGNFWLTKWLSRLLSSNN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVNSGKRLLLIENSTCSNEVRVALSVNNKVVEFEQEFKEKKQLRGNIYVAYIKRIEPSLQ
CCCCCCEEEEEECCCCCCCEEEEEEECCCEEHHHHHHHHHHHHCCCEEEEEEECCCCCCE
AVFIEYGKNKQGFLSFSEISLDYFNIPEKEKETIFESYSNDDYTEKGNDTIANVSSDSAA
EEEEEECCCCCCCEEHHHEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCC
SKNVNESGSGFVREVPLYKKYKLQDVISVNQKVLVQLTKEERGNKGASFTTYITLVGRYC
CCCCCCCCCCCEEECCCHHHHHHHHHHHCCHHHEEEEHHHHCCCCCCCHHHHHHHHHHHH
VFMPNSMSKGGVSRRIEDANVRKQLKEILSSINLPKRSGLIVRTVGSGKNKKEIEQDYNY
EECCCCCCCCCCHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHH
LSSLWQSIQKNAFSVNVPSLIYNEADVIMRSVRDFCSDGIEVIVSGKEAFEAVRQYANNA
HHHHHHHHHCCCEEEECCHHHHCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCC
LKGSKLRYRLYRGYAPIFTYYGIEDQISELYSNRVELPSGGSLIITLTEAFVSIDVNSGK
CCCCCEEEHHHHCCCCHHEECCCHHHHHHHHHCCCCCCCCCEEEEEEEEEEEEEECCCCC
MTGEDSIEETAYRTNMEAVPEISRQVNLRGLSGLIVVDFIDMLKYQYCRAVESAIKQAFK
CCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
DDKAKVQFSYINDFGLMVFSRQRIKPNIQEINTTECLHCKGTGRVKSNEVIVSSILRDLQ
CCCCEEEEEEECCHHHHEEEHHHCCCCHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHH
HIANKNRNKSFDLVAHSAVIAHIFNNKRNTVSTIEKEFNITLNVSIDNSLDVNTFVLKQG
HHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHEEEEEEEEEECCCCCCEEEEEECC
DDVNLNGYKPLQNSGYQVGDSSNEEADNSNKLRGNFWLTKWLSRLLSSNN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]